Claims
- 1. A modified plant zinc finger protein (ZFP) that binds to a target sequence.
- 2. The modified plant zinc finger protein of claim 1, wherein the target sequence is a nucleic acid sequence.
- 3. The modified plant zinc finger protein of claim 2, wherein the nucleic acid is DNA.
- 4. The modified plant zinc finger protein of claim 2, wherein the target sequence is 3 or more contiguous nucleotides.
- 5. The modified plant zinc finger protein of claim 1 comprising a tandem array of zinc fingers.
- 6. The modified plant zinc finger protein of claim 5, wherein one or more of the zinc fingers of the ZFP are obtained by rational design.
- 7. The modified plant zinc finger protein of claim 5, wherein one or more of the zinc fingers of the ZFP are obtained by selection.
- 8. The modified plant zinc finger protein of claim 7, wherein selection is phage display, interaction trap, ribosome display and RNA-peptide fusion.
- 9. The modified plant zinc finger protein of claim 5, wherein one or more of the zinc fingers comprise canonical C2H2 zinc fingers.
- 10. The modified plant zinc finger protein of claim 5, wherein one or more of the zinc fingers comprise non-canonical zinc fingers.
- 11. The modified plant zinc finger protein of claim 5, wherein one or more of the zinc fingers are derived from two or more plant species.
- 12. The modified plant zinc finger protein of claim 5, wherein one or more amino acid residues are deleted or substituted as compared to a naturally occurring plant ZFP.
- 13. The modified plant zinc finger protein of claim 12, wherein one or more amino acid residues are deleted between one or more of the zinc fingers.
- 14. A fusion polypeptide comprising (i) a modified plant ZFP as described herein and (ii) at least one functional domain.
- 15. The fusion polypeptide of claim 14, wherein the functional domain is a repressive domain.
- 16. The fusion polypeptide of claim 14, wherein the functional domain is a activation domain.
- 17. An isolated polynucleotide encoding the modified plant zinc finger protein of claim 1.
- 18. An expression vector comprising the isolated polynucleotide of claim 17.
- 19. A host cell comprising the isolated polynucleotide of claim 17.
- 20. A method for modulating gene expression in a plant cell; the method comprising contacting the cell with a modified plant zinc finger protein (ZFP) comprising a tandem array of zinc fingers.
CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application claims the benefit of U.S. provisional patent application Serial No. 60/263,445 filed Jan. 22, 2001 and also claims the benefit of U.S. provisional patent application Serial No. 60/290,716 filed May 11, 2001; both of which disclosures are hereby incorporated by reference in their entireties.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60263445 |
Jan 2001 |
US |
|
60290716 |
May 2001 |
US |