Claims
- 1. An antibody capable of enhancing the rate of hydrolysis of a primary amide bond.
- 2. An antibody capable of enhancing the rate of formation of a primary amide bond.
- 3. An antibody of claim 1 or 2 wherein the antibody is produced by the method comprising the steps of:
- (i) generating a gene library of antibody-derived domains;
- (ii) inserting coding DNA for said domains into a phage expression vector; and
- (iii) isolating said catalytic antibodies.
- 4. An antibody of claim 1 or 2 wherein the antibody is produced by the method comprising the steps of:
- (i) immunizing an animal with a composition comprising an analog of a reactant, reaction intermediate, or product of the hydrolysis reaction;
- (ii) removing antibody-producing lymphocytes from said animal; and,
- (iii) fusing the lymphocytes with myeloma cells and thereby producing hybridoma cells producing the antibody.
- 5. An antibody of claim 1 or 2 wherein said primary amide bond is contained in a polypeptide.
- 6. An antibody of claim 1 or 2 wherein said primary amide bond is contained in a C-terminal carboxamide.
- 7. An antibody of claim 1 or 2 wherein said primary amide bond is contained in a side chain of arginine or glutamine.
- 8. An antibody of claim 1 or 2 wherein said primary amide bond is contained in calcitonin.
- 9. An antibody of claim 1 or 2 wherein said primary amide bond is contained in a calcitonin gene-related peptide.
- 10. An antibody of claim 1 or 2 wherein said primary amide bond is contained in big gastrin.
- 11. An antibody of claim 1 or 2 wherein said primary amide bond is contained in bombesin-like peptide.
- 12. An antibody capable of enhancing the rate of hydrolysis or formation of a primary amide bond, said antibody being obtained by the method comprising:
- (i) raising antibodies against a hapten,
- (ii) immobilizing said antibodies,
- (iii) adding a substrate to said antibodies, and
- (iv) identifying antibodies capable of catalyzing the conversion of said substrate to a product.
- 13. The antibody of claim 12 wherein the method further comprises, after step (i),
- (i) concentrating said antibodies and
- (ii) purifying said antibodies.
- 14. A method for providing therapy in an individual in need of said therapy comprising administering to said individual an antibody capable of enhancing the rate of hydrolysis of a primary amide bond.
- 15. A method for providing therapy in an individual in need of said therapy comprising administering to said individual an antibody capable of enhancing the rate of formation of a primary amide bond.
CROSS REFERENCE TO RELATED APPL ICATIONS
This application is the U.S. national stage of PCT/US94/04437, filed Apr. 22, 1994, which is a continuation-in-part of U.S. patent application Ser. No. 08/052,490, filed Apr. 23, 1993, which is a continuation-in-part of U.S. patent application Ser. No. 07/190,271, filed May 4, 1988. U.S. Ser. No. 08/052,490 is also a continuation-in-part of U.S. patent application Ser. No. 07/773,042, filed Oct. 10, 1991, which is a continuation-in-part of U.S. patent application Ser. No. 07/740,501, filed Aug. 5, 1991. U.S. Ser. No. 08/052,490 is also a continuation-in-part of U.S. Ser. No. 07/776,186, filed Sep. 3, 1991 as a continuation-in-part of U.S. Ser. No. 07/190,271, filed May 4, 1988, which is a continuation-in-part of U.S. Ser. No. 06/674,253, filed Nov. 27, 1984, now U.S. Pat. No. 4,888,821, which is a continuation-in-part of U.S. Ser. No. 06/556,016, now abandoned. U.S. Ser. No. 07/740,501 is also a continuation-in-part of U.S. Ser. No. 07/190,271, the lineage of which is set forth hereinabove, as well as a continuation-in-part of U.S. Ser. No. 07/700,210, filed Jun. 12, 1991, and U.S. Ser. No. 07/498,225, filed Mar. 23, 1990.
PCT Information
Filing Document |
Filing Date |
Country |
Kind |
102e Date |
371c Date |
PCT/US94/04437 |
4/22/1994 |
|
|
5/4/1996 |
5/4/1996 |
Publishing Document |
Publishing Date |
Country |
Kind |
WO94/25573 |
|
|
|
US Referenced Citations (1)
Number |
Name |
Date |
Kind |
5126258 |
Lerner et al. |
Jun 1992 |
|
Non-Patent Literature Citations (2)
Entry |
Janda, et. al. (1988)Science 241, 1188-1191. |
Gallacher, G, et. al. (1992) Biochem. J. 284, 675-680. |
Related Publications (4)
|
Number |
Date |
Country |
|
773042 |
Oct 1991 |
|
|
776186 |
Sep 1991 |
|
|
700210 |
Jun 1991 |
|
|
498225 |
Mar 1990 |
|
Continuation in Parts (7)
|
Number |
Date |
Country |
Parent |
052490 |
Apr 1993 |
|
Parent |
190271 |
May 1988 |
|
Parent |
190271 |
May 1988 |
|
Parent |
674253 |
Nov 1984 |
|
Parent |
556016 |
Nov 1983 |
|
Parent |
740501 |
Aug 1991 |
|
Parent |
190271 |
|
|