Claims
- 1. An assay for detection of CD30 or CD30-L in a sample, comprising contacting the sample with a substantially homogeneous purified CD30-L polypeptide capable of binding CD30, wherein said CD30-L polypeptide is encoded by a DNA sequence that will hybridize to the nucleotide sequence presented in SEQ ID NO:19 or SEQ ID NO:23 under severely stringent conditions, and detecting binding of the sample thereto.
- 2. The assay of claim 1 which is a competitive binding assay.
- 3. The assay of claim 2, in which the CD30-L polypeptide is conjugated to a detectable moiety and used to detect binding of the sample to intact cells expressing cell surface CD30.
- 4. The assay of claim 3, in the detectable moiety is selected from the group consisting of radionuclides, chromophores, an enzyme that can catalyze a colorometric reaction, an enzyme that can catalyze a fluorometric reaction, biotin, and avidin.
- 5. The assay of claim 2, in which the CD30-L polypeptide is conjugated to a detectable moiety and used to detect binding of the sample to a CD30 polypeptide bound to a solid phase.
- 6. The assay of claim 5, in the detectable moiety is selected from the group consisting of radionuclides, chromophores, an enzyme that can catalyze a colorometric reaction, an enzyme that can catalyze a fluorometric reaction, biotin, and avidin.
- 7. An assay to detect cells expressing CD30 in a sample, comprising contacting the sample with a substantially homogeneous purified CD30-L polypeptide capable of binding CD30, wherein said CD30-L polypeptide is encoded by a DNA sequence that will hybridize to the nucleotide sequence presented in SEQ ID NO:19 or SEQ ID NO:23 under severely stringent conditions, and detecting binding of the CD30-L polypeptide the cells.
- 8. The assay of claim 7, wherein the CD30-L polypeptide is conjugated to a detectable moiety selected from the group consisting of radionuclides, chromophores, an enzyme that can catalyze a colorometric reaction, an enzyme that can catalyze a fluorometric reaction, biotin, and avidin.
- 9. An assay to detect soluble CD30 in a sample, comprising contacting the sample with a substantially homogeneous purified CD30-L polypeptide capable of binding CD30, wherein said CD30-L polypeptide is encoded by a DNA sequence that will hybridize to the nucleotide sequence presented in SEQ ID NO:19 or SEQ ID NO:23 under severely stringent conditions, and detecting binding of the CD30-L polypeptide to the soluble CD30.
- 10. The assay of claim 9, wherein the CD30-L polypeptide is conjugated to a detectable moiety selected from the group consisting of radionuclides, chromophores, an enzyme that can catalyze a colorometric reaction, an enzyme that can catalyze a fluorometric reaction, biotin, and avidin.
- 11. An assay for detection of CD30-L in a sample, comprising contacting the sample with an antibody immunoreactive with a CD30-L polypeptide encoded by a DNA sequence that will hybridize to the nucleotide sequence presented in SEQ ID NO:19 or SEQ ID NO:23 under severely stringent conditions, and detecting binding of the antibody to the CD30-L.
- 12. The assay of claim 11 wherein the CD30-L is present on cells.
- 13. The assay of claim 12, wherein the antibody is a monoclonal antibody.
Priority Claims (1)
Number |
Date |
Country |
Kind |
PCT/US93/04926 |
May 1993 |
WO |
|
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a divisional of U.S. Ser. No. 09/628,126, filed Jul. 28, 2000, now allowed; which is a divisional of 09/079,785 filed May 15, 1998 and issued as U.S. Pat. No. 6,143,869, on Nov. 7, 2000; which is a divisional 08/580,014 filed Dec. 20, 1995 and issued as U.S Pat. No. 5,753,203 on May 19, 1998; which is a divisional of 08/225,989 filed Apr. 12, 1994 and issued as U.S Pat. No. 5,480,981 on Jan. 2, 1996; which is a continuation-in-part of U.S. Application Ser. No. 07/966,775, filed on Oct. 27, 1992, now abandoned; which is a continuation-in-part of U.S. Application Ser. No. 07/907,224, filed on Jul. 1, 1992, now abandoned; which is a continuation-in-part of U.S. application Ser. No. 07/899,660, filed on Jun. 15, 1992, now abandoned; which is a continuation-in-part of U.S. application Ser. No. 07/892,459, filed on Jun. 2, 1992, now abandoned; which is a continuation-in-part of U.S. application Ser. No. 07/889,717, filed on May 26, 1992, now abandoned. Priority is also claimed from International Application PCT/US93/04926 filed May 25, 1993.
Divisions (4)
|
Number |
Date |
Country |
Parent |
09628126 |
Jul 2000 |
US |
Child |
10677593 |
Oct 2003 |
US |
Parent |
09079785 |
May 1998 |
US |
Child |
09628126 |
Jul 2000 |
US |
Parent |
08580014 |
Dec 1995 |
US |
Child |
09079785 |
May 1998 |
US |
Parent |
08225989 |
Apr 1994 |
US |
Child |
08580014 |
Dec 1995 |
US |
Continuation in Parts (5)
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Number |
Date |
Country |
Parent |
07966775 |
Oct 1992 |
US |
Child |
08225989 |
Apr 1994 |
US |
Parent |
07907224 |
Jul 1992 |
US |
Child |
07966775 |
Oct 1992 |
US |
Parent |
07899660 |
Jun 1992 |
US |
Child |
07907224 |
Jul 1992 |
US |
Parent |
07892459 |
Jun 1992 |
US |
Child |
07899660 |
Jun 1992 |
US |
Parent |
07889717 |
May 1992 |
US |
Child |
07892459 |
Jun 1992 |
US |