Claims
- 1. A ribozyme having the formula: ##STR4##
- 2. A DNA fragment comprising:
- (a) a first DNA sequence encoding hammerhead ribozyme RNA, and
- (b) a second DNA sequence covalently linked to the 3'-terminus of said first DNA sequence, said second DNA sequence encoding
- (b1) a cleavage site at the 3'-terminus site of RNA transcribed from the said first DNA sequence,
- (b2) binding site ribozyme RNA which recognizes and binds to said 3'-terminus site of RNA transcribed from said first DNA sequence, and
- (b3) catalytic hammerhead ribozyme RNA capable of cleaving said transcribed RNA at said 3'-terminus site, wherein said first DNA sequence encodes hammerhead ribozyme RNA which acquires a hairpin configuration due to formation of a nucleotide pair on the 3'-terminus end of said hammerhead ribozyme RNA.
- 3. The DNA fragment of claim 2, comprising the following sequence: ##STR5## wherein the symbol .dwnarw. identifies said cleavage site.
- 4. The DNA fragment of claim 2, having an RNA polymerase promoter sequence operably covalently linked to the 5'-terminus thereof.
- 5. A recombinant vector comprising the DNA fragment of claim 2.
- 6. A recombinant vector comprising an RNA polymerase promoter operably covalently linked to the DNA fragment of claim 2.
- 7. The recombinant vector of claim 5, wherein said DNA has the sequence: ##STR6## wherein the symbol .dwnarw. identifies said cleavage site.
- 8. The recombinant vector of claim 7, further comprising an RNA polymerase promoter sequence operably covalently linked to the 5'-terminus of said DNA sequence.
Priority Claims (1)
Number |
Date |
Country |
Kind |
1-329831 |
Dec 1989 |
JPX |
|
Parent Case Info
This is a continuation of application Ser. No. 07/499,787, filed on Mar. 27, 1990, now abandoned.
Non-Patent Literature Citations (1)
Entry |
Dzianott et al. (1989), Proc. Natl. Acad. Sci., vol. 86, pp. 4823-4827. |
Continuations (1)
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Number |
Date |
Country |
Parent |
499787 |
Mar 1990 |
|