Claims
- 1. A purified and isolated non-naturally occurring RNA ligand to transforming growth factor β2 (TGFβ2) wherein said ligand is selected from the group consisting of the sequences set forth in Tables 5, 7, 8, 11, 13, 14, 16-19, and FIG. 9 (SEQ ID NOS: 21-121 and 128-193).
- 2. The purified and isolated non-naturally occurring RNA ligand of claim 1 comprising SEQ ID NO:115 conjugated to polyethylene glycol (PEG), having the following structure: whereinX=PEG, and LIGAND=rGrGrArGrGfUfUrAfUfUrAfCrArGrArGfUfCfUrGfUfUrArGfCfUrGfUrAfCfUfCfC-3′-3′-dT.
- 3. The purified and isolated non-naturally occurring RNA ligand of claim 2 wherein said PEG has a molecular weight of about between 10-80 K.
- 4. The purified and isolated non-naturally occurring RNA ligand of claim 2 wherein said PEG has a molecular weight of about 20-45 K.
- 5. The purified and isolated non-naturally occurring RNA ligand of claim 2, wherein said ligand is LIGAND=rGrGrArGrGfUfUrAfUfUrAfCrArGrArGfUfCfUrGfUfUrArGfCfUrGfUrAfCfUfCfC-3′-3′-dT (SEQ ID NO:115), wherein rG is 2′OH G, rA is 2′OH A, fU is 2′F U and fC is 2′F C.
RELATED APPLICATIONS
This application is a Continuation-in-Part of U.S. patent application Ser. No. 09/046,247, filed Mar. 23, 1998, U.S. Pat. No. 6,124,449 entitled “High Affinity TGFβ Nucleic Acid Ligands and Inhibitors,” which is a Continuation-in-Part of U.S. patent application Ser. No. 08/458,424, filed Jun. 2, 1995, entitled “High Affinity TGFβ Nucleic Acid Ligands and Inhibitors”, now issued as U.S. Pat. No. 5,731,424, which is a Continuation-in-Part of U.S. patent application Ser. No. 07/714,131, filed Jun. 10, 1991, entitled “Nucleic Acid Ligands”, now issued as U.S. Pat. No. 5,475,096, U.S. patent application Ser. No. 07/931,473, filed Aug. 17, 1992, entitled “Methods for Identifying Nucleic Acid Ligands”, now issued as U.S. Pat. No. 5,270,163, U.S. patent application Ser. No. 07/964,624, filed Oct. 21, 1992, entitled “Nucleic Acid Ligands to HIV-RT and HIV-1 Rev”, now issued as U.S. Pat. No. 5,496,938, and U.S. patent application Ser. No. 08/117,991, filed Sep. 8, 1993, entitled “High Affinity Nucleic Acid Ligands Containing Modified Nucleotides”, now abandoned. U.S. patent application Ser. No. 07/714,131 is a Continuation-in-Part of U.S. patent application Ser. No. 07/536,428, filed Jun. 11, 1990, entitled Systematic Evolution of Ligands by EXponential Enrichment, now abandoned. This application is also a Continuation-in Part of U.S. patent application Ser. No. 08/434,465, filed May 4, 1995, now U.S. Pat. No. 6,011,020, entitled “Nucleic Acid Ligand Complexes,” issued Jan. 4, 2000.
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Continuation in Parts (10)
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Date |
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Parent |
09/046247 |
Mar 1998 |
US |
Child |
09/363939 |
|
US |
Parent |
08/458424 |
Jun 1995 |
US |
Child |
09/046247 |
|
US |
Parent |
07/714131 |
Jun 1991 |
US |
Child |
08/458424 |
|
US |
Parent |
09/363939 |
|
US |
Child |
08/458424 |
|
US |
Parent |
08/117991 |
Sep 1993 |
US |
Child |
09/363939 |
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US |
Parent |
07/964624 |
Oct 1992 |
US |
Child |
08/117991 |
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US |
Parent |
07/931473 |
Aug 1992 |
US |
Child |
07/964624 |
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US |
Parent |
07/536428 |
Jun 1990 |
US |
Child |
07/714131 |
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US |
Parent |
09/363939 |
|
US |
Child |
07/714131 |
|
US |
Parent |
08/434465 |
May 1995 |
US |
Child |
09/363939 |
|
US |