Method for Inspecting a Grating Biochip

Information

  • Patent Application
  • 20070156349
  • Publication Number
    20070156349
  • Date Filed
    December 22, 2006
    17 years ago
  • Date Published
    July 05, 2007
    17 years ago
Abstract
A method for inspecting a grating biochip comprises the steps of irradiating a grating biochip using a light beam, measuring a diffracted light using a photodetector, selecting a plurality of parameters of the grating biochip, and optimizing the parameters to enhance the detection sensitivity, wherein the diffracted light is generated by the light beam passing the grating biochip. The grating biochip comprises a grating structure including a semiconductor substrate, a grating positioned on the semiconductor substrate and a dielectric layer covering the grating and the semiconductor substrate. The sample of the biochip is positioned on the grating structure.
Description

BRIEF DESCRIPTION OF THE DRAWINGS

The objectives and advantages of the present invention will become apparent upon reading the following description and upon reference to the accompanying drawings in which:



FIG. 1 shows a conventional prism coupled surface plasma resonance inspection;



FIG. 2 illustrates a biochip according to one embodiment of the present invention;



FIG. 3(
a) illustrates a biochip inspection system according to one embodiment of the present invention;



FIG. 3(
b) illustrates a signature obtained by inspecting a biochip sample using the inspection system shown in FIG. 3(a);



FIG. 4 illustrates a diffraction light spectrum of the angular scatterometer as the variation of the sample refractive index is 0.01;



FIG. 5 illustrates a diffraction light spectrum of the prism coupled surface plasma resonance as the variation of the sample refractive index is 0.01;



FIG. 6 illustrates a flow chart showing an optimization method for the biochip according to one embodiment of the present invention;



FIG. 7 illustrates a sensitivity distribution diagram of each subordinate parameter combination obtained by RCWA at fixed primary parameters;



FIG. 8 illustrates a distribution diagram of the sensitivity corresponding to the primary parameter obtained by integrating multiple sensitivity distribution diagrams; and



FIG. 9 illustrates a comparison result for measurements of the biochip sample by the angular scatterometer and the prism coupled surface resonance.


Claims
  • 1. A method for inspecting a grating biochip, comprising: irradiating a light beam to a grating biochip;measuring a diffracted light by using a photodetector, wherein the diffracted light is generated by the light beam passing the grating biochip;selecting a plurality of parameters of the grating biochip; andoptimizing the parameters to enhance detection sensitivity.
  • 2. The method for inspecting a grating biochip as claimed in claim 1, wherein the light beam is generated by a focused laser source, and a measurement signal generated by the photodetector is a function of an incident angle of the light beam and structural parameters of the grating biochip.
  • 3. The method for inspecting a grating biochip as claimed in claim 1, wherein the light beam is generated by a linear laser source, and a measurement signal generated by the photodetector is a function of an incident angle of the light beam and structural parameters of the grating biochip.
  • 4. The method for inspecting a grating biochip as claimed in claim 1, wherein the light beam is generated by a planar laser source, and a measurement signal generated by the photodetector is a function of an incident angle of the light beam and structural parameters of the grating biochip.
  • 5. The method for inspecting a grating biochip as claimed in claim 1, wherein the grating biochip comprises a grating structure including: a semiconductor substrate;a grating positioned on the semiconductor substrate; anda dielectric layer covering the grating and the semiconductor substrate.
  • 6. The method for inspecting a grating biochip as claimed in claim 5, wherein the dielectric constant of the dielectric layer is higher than that of the grating.
  • 7. The method for inspecting a grating biochip as claimed in claim 5, wherein the grating biochip further comprises a single sample positioned on the grating structure.
  • 8. The method for inspecting a grating biochip as claimed in claim 5, wherein the grating biochip comprises a plurality of grating structures and samples arranged in a one-dimensional array.
  • 9. The method for inspecting a grating biochip as claimed in claim 5, wherein the grating biochip comprises a plurality of grating structures and samples arranged in a two-dimensional array.
  • 10. The method for inspecting a grating biochip as claimed in claim 5, wherein the grating is made of silicon-oxygen compound.
  • 11. The method for inspecting a grating biochip as claimed in claim 5, wherein the grating is made of silicon-nitrogen compound.
  • 12. The method for inspecting a grating biochip as claimed in claim 5, wherein the dielectric layer is made of silicon-nitrogen compound.
  • 13. The method for inspecting a grating biochip as claimed in claim 5, wherein the dielectric layer is made of poly-silicon material.
  • 14. The method for inspecting a grating biochip as claimed in claim 1, wherein the step of optimizing the parameters comprises: determining a primary parameter and a plurality of subordinate parameters from the parameters;using a rigorous coupled wave algorithm to find a first subordinate parameter combination enabling the grating biochip to have the first optimal sensitivity as the primary parameter is a first default value;using the rigorous coupled wave algorithm to find a second subordinate parameter combination enabling the grating biochip to have a second optimal sensitivity as the primary parameter is a second default value; andcomparing the first optimal sensitivity with the second optimal sensitivity to determine an optimal subordinate parameter combination.
  • 15. The method for inspecting a grating biochip as claimed in claim 14, wherein the grating biochip includes a grating, and one of the parameters is the period of the grating.
  • 16. The method for inspecting a grating biochip as claimed in claim 14, wherein the grating biochip includes a grating, and one of the parameters is the line/space ratio of the grating.
  • 17. The method for inspecting a grating biochip as claimed in claim 14, wherein the grating biochip includes a grating, and one of the parameters is the thickness of the grating.
  • 18. The method for inspecting a grating biochip as claimed in claim 14, wherein the grating biochip includes a dielectric layer, and one of the parameters is the thickness of the dielectric layer.
Priority Claims (1)
Number Date Country Kind
094147872 Dec 2005 TW national