Claims
- 1. An isolated polynucleotide with C-type lectin receptor activity comprising a nucleotide sequence of SEQ ID NO: 2, 3, or 12, the mature protein coding portion thereof.
- 2. An isolated polynucleotide encoding a polypeptide with biological activity, wherein said polynucleotide hybridizes to the complement of a polynucleotide of claim 1 under stringent hybridization conditions.
- 3. An isolated polynucleotide encoding a polypeptide with biological activity, said polynucleotide having greater than about 99% sequence identity with the polynucleotide of claim 1.
- 4. The polynucleotide of claim 1 which is a DNA.
- 5. An isolated polynucleotide which comprises the complement of the polynucleotide of claim 1.
- 6. A vector comprising the polynucleotide of claim 1.
- 7. An expression vector comprising the polynucleotide of claim 1.
- 8. A host cell genetically engineered to express the polynucleotide of claim 1.
- 9. A host cell genetically engineered to contain the polynucleotide of claim 1 in operative association with a regulatory sequence that controls expression of the polynucleotide in the host cell.
- 10. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample with a compound that binds to and forms a complex with the polynucleotide of claim 1 for a period sufficient to form the complex; and b) detecting the complex, so that if a complex is detected, the polynucleotide of claim 1 is detected.
- 11. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample under stringent hybridization conditions with nucleic acid primers that anneal to the polynucleotide of claim 1 under such conditions; b) amplifying a product comprising at least a portion of the polynucleotide of claim 1; and c) detecting said product and thereby the polynucleotide of claim 1 in the sample.
- 12. The method of claim 11, wherein the polynucleotide is an RNA molecule that encodes a polypeptide selected from the group of SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 13, SEQ ID NO: 14, SEQ ID NO: 15, and the method further comprises reverse transcribing an annealed RNA molecule into a cDNA polynucleotide.
- 13. A nucleic acid array comprising the polynucleotide of claim 1 or a unique segment of the polynucleotide of claim 1 attached to a surface.
- 14. The array of claim 13, wherein the array detects full-matches to the polynucleotide or a unique segment of the polynucleotide of claim 1.
- 15. The array of claim 13, wherein the array detects mismatches to the polynucleotide or a unique segment of the polynucleotide of claim 1.
1. CROSS REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part application of U.S. application Ser. No. 09/545,283, filed Apr. 07, 2000, entitled “Methods and Materials Relating to Novel C-type Lectin Receptor-like Polypeptides and Polynucleotides,” Attorney Docket No. HYS-5, which in turn is a continuation-in-part of U.S. application Ser. No. 09/496,914, filed Feb. 03, 2000, entitled “Novel Contigs Obtained from Various Libraries,” Attorney Docket No. 787, both of which are incorporated herein by reference in their entirety.
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
09545283 |
Apr 2000 |
US |
Child |
10212198 |
Aug 2002 |
US |
Parent |
09496914 |
Feb 2000 |
US |
Child |
09545283 |
Apr 2000 |
US |