Claims
- 1. An isolated polynucleotide with human growth regulatory protein precursor-like polypeptide activity comprising a nucleotide sequence of SEQ ID NO: 2 or 3, the mature protein coding portion thereof, or the active domain thereof.
- 2. An isolated polynucleotide encoding a polypeptide with biological activity, said polynucleotide which hybridizes to the complement of a polynucleotide of claim 1 under stringent hybridization conditions.
- 3. An isolated polynucleotide encloding a polypeptide with biological activity, said polynucleotide having greater than about 90% sequence identity with the polynucleotide of claim 1.
- 4. The polynucleotide of claim 1 which is a DNA sequence.
- 5. An isolated polynucleotide which comprises the complement of the polynucleotide of claim 1.
- 6. A vector comprising the polynucleotide of claim 1.
- 7. An expression vector comprising the polynucleotide of claim 1.
- 8. A host cell genetically engineered to express the polynucleotide of claim 1.
- 9. A host cell genetically engineered to contain the polynucleotide of claim 1 in operative association with a regulatory sequence that controls expression of the polynucleotide in the host cell.
- 10. An isolated polypeptide comprising an amino acid selected from the group consisting of SEQ ID NO: 4, SEQ ID NO: 5, the mature protein portion thereof, and active domain thereof.
- 11. A composition comprising the polypeptide of claim 10 and a carrier.
- 12. An antibody directed against the polypeptide of claim 10.
- 13. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample with a compound that binds to and forms a complex with the polynucleotide of claim 1 for a period sufficient to form the complex; and b) detecting the complex, so that if a complex is detected, the polynucleotide of claim 1 is detected.
- 14. A method for detecting the polynucleotide of claim 1 in a sample, comprising:
a) contacting the sample under stringent hybridization conditions with nucleic acid primers that anneal to the polynucleotide of claim 1 under such conditions; b) amplifying a product comprising at least a portion of the polynucleotide of claim 1; and c) detecting said product and thereby the polynucleotide of claim 1 in the sample.
- 15. The method of claim 14, wherein the polynucleotide is an RNA molecule that encodes a polypeptide of claim 10, and the method further comprises reverse transcribing an annealed RNA molecule into a cDNA polynucleotide.
- 16. A method for detecting the polypeptide of claim 10 in a sample, comprising:
a) contacting the sample with a compound that binds to and forms a complex with the polypeptide under conditions and for a period sufficient to form the complex; and b) detecting formation of the complex, so that if a complex formation is detected, the polypeptide of claim 10 is detected.
- 17. A method for identifying a compound that binds to the polypeptide of claim 10, comprising:
a) contacting the compound with the polypeptide of claim 10 under conditions and for a time sufficient to form a polypeptide/compound complex; and b) detecting the complex, so that if the polypeptide/compound complex is detected, a compound that binds to the polypeptide of claim 10 is identified.
- 18. A method for identifying a compound that binds to the polypeptide of claim 10, comprising:
a) contacting the compound with the polypeptide of claim 10, in a cell, for a time sufficient to form a polypeptide/compound complex, wherein the complex drives expression of a reporter gene sequence in the cell; and b) detecting the complex by detecting reporter gene sequence expression, so that if the polypeptide/compound complex is detected, a compound that binds to the polypeptide of claim 10 is identified.
- 19. A method of producing the polypeptide of claim 10, comprising,
a) culturing the host cell of claim 8 for a period of time sufficient to express the polypeptide in said cell; and b) isolating the polypeptide from the cell culture or cells of step (a).
- 20. A kit comprising the polypeptide of claim 10.
- 21. A nucleic acid array comprising the polynucleotide of claim 1 or a unique segment of the polynucleotide of claim 1 attached to a surface.
- 22. The array of claim 21, wherein the array detects full-matches to the polynucleotide or a unique segment of the polynucleotide of claim 1.
- 23. The array of claim 21, wherein the array detects mismatches to the polynucleotide or a unique segment of the polynucleotide of claim 1.
- 24. A method of treatment comprising administering to a mammalian subject in need thereof a therapeutic amount of a composition comprising a polypeptide of claim 10 and a pharmaceutically acceptable carrier.
- 25. A polypeptide having growth regulatory protein activity comprising at least ten consecutive amino acids from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 5.
- 26. The polypeptide of claim 26, comprising at least five consecutive amino acids from the group consisting of SEQ ID NO: 4 and SEQ ID NO: 5.
- 27. A polynucleotide encoding a polypeptide according to claim 25.
- 28. A polynucleotide encoding a polypeptide according to claim 26.
- 29. A polynucleotide encoding a polypeptide according to claim 10.
1. CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation-in-part application of U.S. application Ser. No. 09/563,786, filed on May 2, 2000, entitled “Methods and Materials Relating to Novel Growth Regulatory-like Polypeptides and Polynucleotides,” Attorney Docket No. HYS-7, which is a continuation-in-part application of U.S. application Ser. No. 09/491,404, filed on Jan. 25, 2000, entitled “Novel Contigs Obtained from Various Libraries,” Attorney Docket No. 785, both of which are herein incorporated by reference in their entirety.
Continuation in Parts (3)
|
Number |
Date |
Country |
Parent |
PCT/US01/02455 |
Jan 2001 |
US |
Child |
10216038 |
Aug 2002 |
US |
Parent |
09563786 |
May 2000 |
US |
Child |
PCT/US01/02455 |
Jan 2001 |
US |
Parent |
09491404 |
Jan 2000 |
US |
Child |
09563786 |
May 2000 |
US |