Patent Grant
8715654
A long-standing dilemma in tumor immunology is the ability of solid tumor cells to escape immune surveillance despite demonstrable antitumor T-cell response. Primarily, the immune evasion mechanism of tumor has been evaluated in the context of expression of immunosuppressive bio-molecules viz., IL-10, transforming growth factor-b (TGF-b), indoleamine-2,3-deoxygenase (IDO), macrophage colony stimulating factor (M-CSF), arginase, prostaglandin E2 (PGE2), cyclooxygenase-2 (COX2) and nitric-oxide synthase 2 (NOS2), IL-6, chemokine CXCL12 and the like, that inhibit the function of dendritic cells (DC) and T cells. The increased expression of death inducing molecules (FasL & TRAIL), which induces apoptosis in tumor infiltrating T cells, has also been elucidated to explain the mechanism by which tumors evade the immune system.
The migration of immune cells to a target site is a major step in eliciting the immune response against tumor cell. Chemotaxis, or the oriented movement of a cell in response to a chemical agent, is a complex and highly integrated process. The movement can be positive (toward) or negative (away) from a chemical gradient. Movement toward an agent or stimulus is termed positive chemotaxis (i.e., the agent or stimulus is chemoattractive for the cell), while movement away from an agent or stimulus is termed negative chemotaxis (i.e., the agent or stimulus is chemorepulsive for the cell). It is believed that for both prokaryotes and eukaryotes, cells undergoing chemotaxis sense a change in agent concentration and, thereby, move in response to the concentration gradient. Chemoattraction (CA) and chemorepulsion (CR) are therefore properties of the agent or stimulus, while chemotaxis is a property of cells.
The present inventors have discovered proteins which are expressed (secreted) by tumor cells which keep anti-tumor T cells (CD4 & CD8), neutrophils, NK cells at the bay while concomitantly recruiting regulatory T cells at tumor sites and thus mediating evasion of the immune response. It would be advantageous to identify these proteins released from cancer cells that induce negative chemotaxis of immune cells and/or inhibit the activity of these proteins in order to induce positive chemotaxis of immune cells toward cancer cells.
The present invention provides methods of inducing the migration of an immune cell toward a cancer cell comprising inhibiting the activity of a chemorepellant released from the cancer cell.
In some embodiments, the activity of a chemorepellant released from a human cancer cell is inhibited. In other embodiments, the human cancer cell is selected from the group consisting of a renal adenocarcinoma cell, renal carcinoma cell, a glioblastoma cell a colon carcinoma cell, a hepatocellular carcinoma cell, an ovarian carcinoma cell and a prostate cancer cell.
In one embodiment, the activity of a chemorepellant released from the cancer cell is inhibited, wherein the chemorepellant comprises a sequence that has substantial identity to a protein isolated from ovarian cancer cystic fluid or to a biologically active fragment thereof, wherein the isolated protein or fragment thereof is capable of inducing chemorepulsion of an immune cell. In another embodiment, the chemorepellant comprises a sequence that has substantial identity to a protein isolated from a supernatant of a cell line or to a biologically active fragment thereof, wherein the cell line is selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell.
In another embodiment, the chemorepellant has substantial identity to the protein isolated from an ovarian cystic fluid, or to a biologically active fragment thereof. In another embodiment, the chemorepellant has substantial identity to of a protein isolated from a supernatant of a cell line, or a biologically active fragment thereof, wherein the cell line is selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell.
In one embodiment, the chemorepellant has substantial identity to a protein selected from a chemorepellant protein set forth in Tables 1 to 9, or a biologically active fragment of thereof. In an additional embodiment, the chemorepellant has substantial identity to a protein selected from a protein set forth in Table 10 to 11, or a biologically active fragment thereof. In another embodiment, the chemorepellant has substantial identity to a protein selected from the group selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein, or a biologically active fragment of any of thereof.
In yet another embodiment, the invention is a method of treating cancer in a patient in need thereof comprising inhibiting the activity of a chemorepellant released from a cancer cell.
In a further embodiment, the invention is a method of inducing negative chemotaxis of a human immune cell comprising administering an inventive chemorepellant. In some embodiments, the chemorepellant comprises a sequence that has substantial identity to a protein isolated from ovarian cancer cystic fluid or to a biologically active fragment thereof, wherein the isolated protein or fragment thereof is capable of inducing chemorepulsion of an immune cell. In another embodiment, the invention is a method of inducing negative chemotaxis of a human immune cell comprising administering a chemorepellant, wherein the chemorepellant comprises a sequence that has substantial identity to a protein isolated from a supernatant of a cell line selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell, or a biologically active fragment of said isolated protein, wherein said protein or fragment thereof is capable of inducing negative chemotaxis. In an additional embodiment, the administered chemorepellant comprises a sequence that has substantial identity to a protein listed in Tables 1 to 9, or to a biologically active fragment thereof. In an additional embodiment, the administered chemorepellant has substantial identity to a protein listed in Tables 10 to 11, or to a biologically active fragment thereof. In yet another embodiment, the administered chemorepellant comprises a sequence that has substantial identity to a protein selected from the group selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein, or a biologically active fragment thereof.
In yet another embodiment, the invention is a method of treating a condition mediated by migration of a human migratory cell toward a chemotactic site comprising administering to said patient a therapeutically effective amount of an inventive chemorepellant. In some embodiments, the chemorepellant comprises a sequence that has substantial identity to a protein isolated from ovarian cancer cystic fluid, or to a biologically active fragment thereof, wherein the isolated protein or fragment thereof is capable of inducing chemorepulsion of an immune cell. In a further embodiment, the invention is a method of treating a condition mediated by migration of a human migratory cell toward a chemotactic site comprising administering to said patient a therapeutically effective amount of a chemorepellant, wherein said chemorepellant comprises a sequence that has substantial identity to a protein isolated from a supernatant of a cell line selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell, or to a biologically active fragment of any of thereof, wherein the protein or fragment thereof is capable of inducing negative chemotaxis of an immune cell.
These and other aspects of the invention, as well as various advantages and utilities, will be more apparent with reference to the drawings and the detailed description of the embodiments of the invention.
A description of the embodiments of the invention follows.
As used herein, “a” or “an” are taken to mean one or more unless otherwise specified.
The present invention is based on the surprising discovery that one or more proteins isolated from ovarian cancer cystic fluid and/or from the supernatants of human cancer cell cultures induce negative chemotaxis of neutrophils. For example, as shown in Example 1, neutrophils contacted with certain chromatographic fractions of ovarian cancer cystic fluid showed greater than 9-fold induction of chemotaxis than that in response to media.
In one embodiment, the invention is a method of inducing migration of an immune cell toward a cancer cell comprising inhibiting the activity of a chemorepellant released from the cancer cell. In some embodiments, the cancer cell is selected from the group consisting of colon carcinoma cell, prostate cancer cell, breast cancer cell, lung cancer cell, skin cancer cell, liver cancer cell, bone cancer cell, pancreas cancer cell, ovarian cancer cell, testicular cancer cell, bladder cancer cell, kidney cancer cell, brain cancer cell, glioma cell, head and neck cancer cell. In another embodiment, the cancer cell is a renal adenocarcinoma cell, renal carcinoma cell, a glioblastoma cell a colon carcinoma cell, a hepatocellular carcinoma cell, an ovarian carcinoma cell and a prostate cancer cell.
According to the present method, migration of an immune cell toward a cancer cell can be induced by inhibiting the activity of a chemorepellant released from the cancer cell. The chemorepellant released from the cancer cell is a protein that induces negative chemotaxis of an immune cell. The inventive methods also encompass a method of inducing negative chemotaxis of an immune cell comprising administering a chemorepellant, wherein the chemorepellant comprises a sequence that has substantial identity to a protein release from a cancer cell, or a biologically active fragment thereof.
A “chemorepellant” is an agent or stimulus that induces, elicits or triggers negative chemotaxis of a migratory cell (movement away from an agent or stimulus). In one embodiment, the chemorepellant comprises an amino acid sequence that has substantial identity to a protein isolated from ovarian cancer cystic fluid, or to a biologically active fragment thereof, wherein the isolated protein or fragment thereof is capable of inducing chemorepulsion of an immune cell. In another embodiment, the chemorepellant has substantial identity to a protein isolated from ovarian cancer cystic fluid or to a biologically active fragment thereof. In an additional embodiment, the chemorepellant has substantial identity to a protein isolated from ovarian cancer cystic fluid.
In another embodiment, the chemorepellant comprises a sequence that has substantial identity to a protein isolated from a supernatant of a cell line selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell, or a biologically active fragment of said isolated protein, wherein said protein or fragment thereof is capable of inducing negative chemotaxis. In yet another embodiment, the chemorepellant has substantial identity to a protein isolated from a supernatant of a cell line selected from the group consisting of a human renal adenocarcinoma cell, a human renal carcinoma cell, a human glioblastoma cell, a human colon carcinoma cell, a human hepatocellular carcinoma cell, a human ovarian carcinoma cell and a human prostate cancer cell, or a biologically active fragment of said isolated protein.
In yet another embodiment, the chemorepellant comprises a sequence that has substantial identity to a protein set forth in Tables 1 through 9 (shown below in Examples 1 to 3), or to a biologically active fragment thereof. In a further embodiment, the chemorepellant has substantial identity to a protein set forth in Tables 1 through 9, or a biologically active fragment thereof. In yet another embodiment, the chemorepellant is a protein set forth in Tables 1 through 9. In another embodiment, the chemorepellant is a protein set forth in Tables 10 to 11.
In an additional embodiment, the chemorepellant protein is a protein that is released by at least two distinct cancer cells. Cancer cells are distinct when they are of different origin or different cancer cell types. For example, liver cancer cells and ovarian cancer cells are distinct cancer cells. Similarly, a cancer cell of the kidney cancer cell line, ACHN, is distinct from the kidney cancer cell line 786-O. In a further embodiment, the chemorepellant protein has substantial identity to a protein set forth in Tables 10-11, or to a biologically active fragment thereof.
In another embodiment, the chemorepellant comprises a sequence that has substantial identity to the amino acid sequence of a protein selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein, or a biologically active fragment of any of thereof. In an additional embodiment, the chemorepellant has substantial identity to a protein selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein. In a further embodiment, the chemorepellant is a protein selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein. Accession Numbers for these proteins are shown below in Tables 1 through 9.
A biologically active fragment is a peptide fragment of a naturally occurring protein or the full-length protein that retains at least some of the biological activity of the naturally occurring protein or the full-length protein. In some embodiments, the biological activity is the ability to induce chemorepulsion of a human migratory cell.
Ovarian cancer cystic fluid refers to cystic fluid from patients with ovarian carcinomas.
In some embodiments, the chemorepellant comprises a sequence that has substantial identity to a protein isolated from the supernatant of a cancer cell culture, wherein the culture is of a human cancer cell selected from the group consisting of a renal adenocarcinoma cell, renal carcinoma cell, a glioblastoma cell a colon carcinoma cell, a hepatocellular carcinoma cell, an ovarian carcinoma cell and a prostate cancer cell. In one embodiment, the human renal adenocarcinoma cell line is ACHN. In another embodiment, the human renal carcinoma cell line is 786-O. In another embodiment, the human glioblastoma cell line is SF539 or U251. In an additional embodiment, the human colon carcinoma cell line is HCC-2998. In a further embodiment, the human hepatocellular carcinoma cell line is HepG2 (ATCC No. HB-8065). In yet another embodiment, the human ovary clear cell carcinoma cell line is ATCC No. CRL-1978. In an additional embodiment, the human prostate cancer cell line is PC3 (ATCC No. CRL-1435).
In certain embodiments of the invention, the chemorepellant comprises a sequence that has substantial identity to the amino acid sequence of a protein isolated from ovarian cancer cystic fluid or the supernatant of a cancer cell line. In these embodiments, the ovarian cancer cystic fluid or supernatant is fractionated and the protein is isolated from a chemorepulsive fraction. A chemorepulsive fraction is a fraction that induces chemorepulsion of a human migratory cell. The ovarian cystic fluid or supernatant can be fractionated, for example, by size exclusion and anion exchange chromatography.
Exemplary amino acid sequences for actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein are shown below:
As used herein, a chemorepellant has “substantial identity” to another protein when the chemorepellant has an amino acid sequence that has at least about 60 percent sequence identity, at least about 70 percent sequence identity, at least about 80 percent sequence identity, at least about 85 percent sequence identity, at least about 85 to 95 percent sequence identity, at least about 90 to about 95 percent sequence identity, at least about 98 percent sequence identity, or at least about 99 percent sequence identity to the amino acid sequence of the other protein. The terms “sequence identity” or “identity” in reference to a sequence refers to sequence identity between two amino acid sequences or between two nucleotide sequences. Identity can be determined by comparing a position in each sequence which may be aligned for purposes of comparison. The terms “sequence homology” or “homology” in reference to a sequence refers to sequence homology between two amino acid sequences or two nucleotide sequences. When an equivalent position in the compared sequences is occupied by the same base or amino acid, then the molecules are identical at that position; when the equivalent site occupied by the same or a similar amino acid residue (e.g., similar in steric and/or electronic nature), then the molecules can be referred to as homologous (similar) at that position. Expression as a percentage of homology, similarity, or identity refers to a function of the number of identical or similar amino acids at positions shared by the compared sequences. Expression as a percentage of homology, similarity, or identity refers to a function of the number of identical or similar amino acids at positions shared by the compared sequences. Various alignment algorithms and/or programs may be used, including FASTA, BLAST, or ENTREZ. FASTA and BLAST are available as a part of the GCG sequence analysis package (University of Wisconsin, Madison, Wis.), and can be used with, e.g., default settings. ENTREZ is available through the National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, Md. In one embodiment, the percent identity of two sequences can be determined by the GCG program with a gap weight of 1, e.g., each amino acid gap is weighted as if it were a single amino acid or nucleotide mismatch between the two sequences.
A “chemoattractant” is an agent or stimulus that induces, elicits or triggers positive chemotaxis (movement towards an agent or stimulus) by a migratory cell.
As used herein the terms “induce,” “elicit,” and “trigger,” when referring to the activity of a chemorepellant or chemoattractant with respect to negative or positive chemotaxis, carry the same meaning.
The activity of the chemorepellant released from a cancer cell is inhibited when the ability of the chemorepellant to induce negative chemotaxis of the immune cell is suppressed or decreased. According to the current invention, the activity of the chemorepellant released from the cancer cell can be inhibited by any means that suppresses negative chemotaxis of the immune cell or that induces positive chemotaxis of the immune cell toward the cancer cell. For example, the activity of the chemorepellant can be inhibited by administering an agent that inhibits the activity of the chemorepellants. Such agents, include, but are not limited to, small molecules, proteins, antibodies, and antisense nucleic acids.
In one embodiment, the activity of the chemorepellant released from a cancer cell is inhibited when the release of the chemorepellant is suppressed or decreased. In another embodiment, the activity of the chemorepellant released from a cancer cell is inhibited by administering an agent that binds to the chemorepellant and inhibits its activity. In some embodiments, the activity of the chemorepellant is inhibited by administering an antibody that binds the chemorepellant and inhibits chemorepellant activity. The term “antibody” as used herein refers to a polypeptide comprising a framework region from an immunoglobulin gene or fragments thereof that specifically binds and recognizes an antigen. The term antibody, as used herein, includes antibody fragments either produced by modification of whole antibodies, or those synthesized de novo using recombinant DNA methodologies (e.g., single chain Fv) (scFv) or those identified using phase display libraries (see, for example, McCafferty et al. (1990) Nature 348:552-554). The term antibody also encompasses both monoclonal and polyclonal antibodies. The terms polyclonal and monoclonal refer to the degree of homogeneity of an antibody preparation, and are not intended to be limited to particular methods of production. In one embodiment, the antibody does not bind other proteins or molecules other than the chemorepellant.
Antibodies can be raised against an appropriate immunogen, including a chemorepellant released from a cancer cell or a fragment thereof. Preparation of immunizing antigen, and polyclonal and monoclonal antibody production can be performed using any suitable technique. A variety of methods have been described (see e.g., Kohler et al., Nature, 256:495-497 (1975)) and Eur. J. Immunol. 6:511-519 (1976)); Milstein et al., Nature 266:550-552 (1977)); Koprowski et al., U.S. Pat. No. 4,172,124; Harlow, E. and D. Lane, 1988, Antibodies: A Laboratory Manual, (Cold Spring Harbor Laboratory: Cold Spring Harbor, N.Y.); and Current Protocols In Molecular Biology, Vol. 2 (Supplement 27, Summer '94), Ausubel, F. M. et al., Eds., (John Wiley & Sons: New York, N.Y.), Chapter 11, 1991); the teachings of each of which are incorporated herein by reference). Other suitable methods of producing or isolating antibodies of the requisite specificity can used, including, for example, methods which select recombinant antibody from a library, or which rely upon immunization of transgenic animals (e.g., mice) capable of producing a full repertoire of human antibodies (see e.g., Jakobovits et al., Proc. Natl. Acad. Sci. USA, 90:2551 2555 (1993)); Jakobovits et al., Nature, 362:255 258 (1993)); Lonberg et al., U.S. Pat. No. 5,545,806; and Surani et al., U.S. Pat. No. 5,545,807; the teachings of which are each incorporated herein by reference). Single-chain antibodies, and chimeric, humanized or primatized (CDR-grafted), or veneered antibodies, as well as chimeric, CDR-grafted or veneered single-chain antibodies, comprising portions derived from different species, and the like are also encompassed by the present invention and the term “antibody.” The various portions of these antibodies can be joined together chemically by conventional techniques, or can be prepared as a contiguous protein using genetic engineering techniques. For example, nucleic acids encoding a chimeric or humanized chain can be expressed to produce a contiguous protein. See, e.g., Cabilly et al., U.S. Pat. No. 4,816,567; Cabilly et al., European Patent No. 0,125,023 B1; Boss et al., U.S. Pat. No. 4,816,397; Boss et al., European Patent No. 0,120,694 B1; Neuberger, M. S. et al., WO 86/01533; Neuberger, M. S. et al., European Patent No. 0,194,276 B1; Winter, U.S. Pat. No. 5,225,539; Winter, European Patent No. 0,239,400 B1; Queen et al., European Patent No. 0 451 216 B1; and Padlan et al., EP 0 519 596 A1. See also, Newman et al., BioTechnology, 10:1455 1460 (1992), regarding primatized antibody, and Ladner et al., U.S. Pat. No. 4,946,778 and Bird et al., Science, 242:423 426 (1988) regarding single-chain antibodies. In addition, antigen-binding fragments of antibodies, including fragments of chimeric, humanized, primatized, veneered or single-chain antibodies, can also be produced, including, but not limited to, Fv, Fab, Fab′ and F(ab′)2 fragments are encompassed by the invention.
In another embodiment, the activity of the chemorepellant is inhibited by administering an antisense nucleic acid. In this context, the chemorepellant antisense nucleic acid comprises at least six nucleotides that are antisense to a gene or cDNA encoding the chemorepellant released from a cancer cell or a portion thereof. The antisense nucleic acid is capable of hybridizing to a portion of an RNA encoding the chemorepellant. The antisense nucleic acid is a double-stranded or single-stranded oligonucleotide, RNA or DNA or a modification or derivative thereof, and can be directly administered to a cell or produced intracellularly by transcription of exogenous, introduced sequences. In one embodiment, the antisense nucleic acid has from about 6 to about 50 nucleotides. In other embodiment, the antisense nucleic acid has at least 10 nucleotides, at least 15 nucleotides, at least 100 nucleotides, or at least 200 nucleotides. The antisense nucleic acid can be DNA or RNA or chimeric mixtures or derivatives or modified versions thereof and can be single-stranded or double-stranded. In addition, the antisense molecules can be polymers that are nucleic acid mimics, such as PNA, morpholino oligos, and LNA. Other types of antisense molecules include short double-stranded RNAs, known as siRNAs, and short hairpin RNAs, and long dsRNA (greater than 50 base pairs).
In yet another embodiment, the activity of the chemorepellant is inhibited by administering a ribozyme molecule that is designed to catalytically cleave gene mRNA transcripts encoding the chemorepellant. Ribozymes thus prevents translation of the target mRNA and prevents expression of the gene product. Ribozymes are enzymatic RNA molecules capable of catalyzing the specific cleavage of RNA. The mechanism of ribozyme action involves sequence-specific hybridization of the ribozyme molecule to complementary target RNA, followed by an endonucleolytic cleavage event. The composition of ribozyme molecules must include one or more sequences complementary to the target gene mRNA, and must include the well known catalytic sequence responsible for mRNA cleavage.
In another embodiment, the invention is a method of treating cancer in a patient suffering therefrom comprising inducing migration of an immune cell toward a cancer cell by inhibiting the activity of a chemorepellant released from a cancer cell. “Treating” or “treatment” includes preventing or delaying the onset of the symptoms, complications, or biochemical indicia of a disease, alleviating or ameliorating the symptoms or arresting or inhibiting further development of the disease, condition, or disorder. A “patient” refers to a human subject in need of treatment. In specific embodiments, the cancer is a solid tumor. In one embodiment, the solid tumor is selected from the group consisting of colon, prostate, breast, lung, skin, liver, bone, pancreas, ovary, testis, bladder, kidney, brain, head and neck cancer. As used herein, a “therapeutically effective amount” in reference to inhibition of a chemorepellant is an amount sufficient to inhibit negative migration of an immune cell and ameliorate a disease or condition of a patient or achieve a desired outcome. In reference to inducing chemotaxis, a “therapeutically effective amount” is an amount sufficient to induce negative migration of a migratory cell and ameliorate a disease or condition of a patient or achieve a desired outcome.
As used herein, “migratory cells” are those cells which are capable of movement from one place to another in response to a stimulus. Human migratory cells include those involved in the processes of cancer, immunity, angiogenesis or inflammation and also include those identified to play a role in other disease states or conditions. Migratory cells include, but are not limited to, immune cells, hematopoietic cells, neural cells, epithelial cells, mesenchymal cells, stem cells, germ cells and cells involved in angiogenesis.
Immune cells include, but are not limited to, monocytes, Natural Killer (NK) cells, dendritic cells (which could be immature or mature), subsets of dendritic cells including myeloid, plasmacytoid (also called lymphoid) or Langerhans; macrophages such as histiocytes, Kupffer's cells, alveolar macrophages or peritoneal macrophages; neutrophils, eosinophils, mast cells, basophils; B cells including plasma B cells, memory B cells, B-1 cells, B-2 cells; CD45RO (naive T), CD45RA (memory T); CD4 Helper T Cells including Th1, Th2 and Tr1/Th3; CD8 Cytotoxic T Cells, Regulatory T Cells and Gamma Delta T Cells.
Hematopoietic cells include, but are not limited to, pluripotent stem cells, multipotent progenitor cells and/or progenitor cells committed to specific hematopoietic lineages. The progenitor cells committed to specific hematopoietic lineages can be of T cell lineage, B cell lineage, dendritic cell lineage, neutrophil lineage, Langerhans cell lineage and/or lymphoid tissue-specific macrophage cell lineage. The hematopoietic cells can be derived from a tissue such as bone marrow, peripheral blood (including mobilized peripheral blood), umbilical cord blood, placental blood, fetal liver, embryonic cells (including embryonic stem cells), aortal-gonadal-mesonephros derived cells, and lymphoid soft tissue. Lymphoid soft tissue includes the thymus, spleen, liver, lymph node, skin, tonsil and Peyer's patches. In other embodiments, hematopoietic cells can be derived from in vitro cultures of any of the foregoing cells, and in particular in vitro cultures of progenitor cells.
Neural cells are cells of neural origin and include neurons and glia and/or cells of both central and peripheral nervous tissue.
Epithelial cells include cells of a tissue that covers and lines the free surfaces of the body. Such epithelial tissue includes cells of the skin and sensory organs, as well as the specialized cells lining the blood vessels, gastrointestinal tract, air passages, lungs, ducts of the kidneys and endocrine organs.
Mesenchymal cells include, but are not limited to, cells that express typical fibroblast markers such as collagen, vimentin and fibronectin.
Cells involved in angiogenesis are cells that are involved in blood vessel formation and include cells of endothelial origin and cells of mesenchymal origin.
Germ cells are cells specialized to produce haploid gametes.
In certain embodiment, the human migratory cell is an immune cell. In other embodiments, the immune cell is selected from the group consisting of lymphocytes, monocytes, neutrophils, eosinophils and mast cells. In a further embodiment, the immune cell is a neutrophil or an eosinophil.
As used herein, the terms “contact” or “contacting” means the act of touching or bringing together two entities or things in such proximity as will allow an influence of at least one on the other. The definition, while inclusive of physical contact is not so limited.
Based on their ability to induce negative chemotaxis, the chemorepellant proteins or biologically active fragments thereof as described herein are useful for inhibiting the induction of chemotaxis of migratory cells toward a chemotactic site. In one embodiment, the chemorepellant comprises a sequence that has substantial identity to the amino acid sequence of a protein selected from the proteins set forth in Tables 1 to 9, or to a biologically active fragment thereof. In some embodiment, the chemorepellant protein comprises a sequence that has substantial identity to a protein selected from the proteins set forth in Tables 10 to 11, or to a biologically active fragment thereof. In another embodiment, the protein comprises a sequence that has substantial identity to the sequence of a protein selected from the group consisting of actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-2, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein, or to a biologically active fragment of any of thereof. As used herein, a “chemotactic site” is a site that induces positive chemotaxis of migratory cells. Chemotactic sites include sites of inflammation, medical implants, transplants and angiogenesis.
The chemorepellants described herein are useful for inhibiting the induction of chemotaxis of migratory cells toward a site of inflammation. Inhibiting migratory cell chemotaxis toward a site of inflammation can result in a reduction or amelioration of an inflammatory response in situations such as bacterial infection, tissue injury-induced inflammation (e.g., ischemia-reperfusion injury), complement-induced inflammation, oxidative stress (e.g., hemodialysis), immune complex-induced inflammation (e.g., antibody-mediated glomerunephritis), cytokine-induced inflammation (e.g., rheumatoid arthritis), antineutrophil cytoplasmic antibodies and vasculitis (e.g, autoimmunity against neutrophil components), genetic disorders of neutrophil regulations (e.g., hereditary periodic fever syndromes), implant related inflammation, and cystic fibrosis.
In certain embodiments, the invention is a method of treating an inflammatory condition in a patient suffering therefrom comprising administering to said patient a therapeutically effective amount of a chemorepellant described herein. In certain other embodiments, the invention is a method of treating an inflammatory condition in a patient suffering therefrom comprising administering to said patient a therapeutically effective amount of a chemorepellant described herein. Inflammatory conditions include, but are not limited to, appendicitis, peptic, gastric or duodenal ulcers, peritonitis, pancreatitis, acute or ischemic colitis, diverticulitis, epiglottitis, achalasia, cholangitis, cholecystitis, hepatitis, inflammatory bowel disease (including, for example, Crohn's disease and ulcerative colitis), enteritis, Whipple's disease, asthma, chronic obstructive pulmonary disease, acute lung injury, ileus (including, for example, post-operative ileus), allergy, anaphylactic shock, immune complex disease, organ ischemia, reperfusion injury, organ necrosis, hay fever, sepsis, septicemia, endotoxic shock, cachexia, hyperpyrexia, eosinophilic granuloma, granulomatosis, sarcoidosis, septic abortion, epididymitis, vaginitis, prostatitis, urethritis, bronchitis, emphysema, rhinitis, cystic fibrosis, pneumonitis, pneumoultramicroscopic silicovolcanoconiosis, alvealitis, bronchiolitis, pharyngitis, pleurisy, sinusitis, influenza, respiratory syncytial virus, herpes, disseminated bacteremia, Dengue fever, candidiasis, malaria, filariasis, amebiasis, hydatid cysts, burns, dermatitis, dermatomyositis, urticaria, acne, vasulitis, angiitis, endocarditis, arteritis, atherosclerosis, thrombophlebitis, pericarditis, myocarditis, myocardial ischemia, periarteritis nodosa, rheumatic fever, Alzheimer's disease, celiac disease, congestive heart failure, adult respiratory distress syndrome, meningitis, encephalitis, multiple sclerosis, cerebral infarction, cerebral embolism, Guillan-Barre syndrome, neuritis, neuralgia, uveitis, arthritides, arthralgias, osteomyelitis, fasciitis, Paget's disease, gout, periodontal disease, rheumatoid arthritis, synovitis, myasthenia gravis, thryoiditis, systemic lupus erythematosus, Goodpasture's syndrome, Behcet's syndrome, allograft rejection, graft-versus-host disease, Type I diabetes, ankylosing spondylitis, Berger's disease, Type II diabetes, Retier's syndrome, Hodgkins disease and injection site reaction.
Injection site reaction is a term generally used to describe inflammation in and around a site of injection. Injection site reaction has been observed with the injection of numerous pharmaceutical agents including, but not limited, chemotherapeutic drugs, immunomodulator drugs, and vaccines. The present invention encompasses a method for the treatment or reduction of injection site reaction comprising administration of a chemorepellant described herein to the injection site. The chemorepellant can, for example, be administered before, during or after injection. In some embodiments, exenatide or analog thereof can be administered topically at the site of the injection.
In another embodiment, the invention is a method of inhibiting positive chemotaxis toward a medical implant. The medical implant can be contacted or coated with a chemorepellant described herein. The proteins can also be administered locally at the site of the medical implant. A medical implant is defined as a device or entity implanted into a surgically or naturally formed cavity of the body. Medical implants include, but are not limited to, stents, pacemakers, pacemaker leads, defibrillators, drug delivery devices, sensors, pumps, embolization coils, sutures, electrodes, cardiovascular implants, arterial stents, heart valves, orthopedic implants, dental implants, bone screws, plates, catheters, cannulas, plugs, fillers, constrictors, sheets, bone anchors, plates, rods, seeds, tubes, or portions thereof. In addition to the chemorepellant, the medical implant can be coated with a cell-growth potentiating agent, an anti-infective agent and/or an anti-inflammatory agent.
In yet another embodiment, the invention is a method of inhibiting positive chemotaxis toward an organ transplant or tissue graft. Organ transplants and tissue grants include, but are not limited to, renal, pancreatic, hepatic, lymphoid and cardiac grafts and organs. Lymphoid grafts include a splenic graft, a lymph node derived graft, a Peyer's patch derived graft, a thymic graft and a bone marrow derived graft. In an additional embodiment, the invention is a method of treating a patient suffering from transplant or graft rejection comprising administering an inventive chemorepellant.
As discussed above, the inventive chemorepellants can be used to inhibit chemotaxis toward a site of angiogenesis. A site of angiogenesis is a site where blood vessels are being formed. In one embodiment, the invention is a method of inducing negative chemotaxis of endothelial cells away from a site of angiogenesis. The invention also encompasses a method of inhibiting angiogenesis in a patient in need thereof comprising administering an inventive chemorepellant In a further embodiment, the invention is a method of treating cancer or a tumor comprising administering an inventive chemorepellant in an amount effective to inhibit angiogenesis. According to another aspect of the invention, a method of inhibiting endothelial cell migration to a tumor site in a subject is provided. The method involves locally administering to or contacting an area surrounding a tumor site in need of such treatment an inventive chemorepellant in an amount effective to inhibit endothelial cell migration into the tumor site in the subject.
Exemplary cancers and tumors that can be treated according to the methods of the invention include, for example, biliary tract cancer; brain cancer including glioblastomas and medulloblastomas; breast cancer; cervical cancer; choriocarcinoma; colon cancer; endometrial cancer; esophageal cancer, gastric cancer; hematological neoplasms, including acute lymphocytic and myelogenous leukemia; multiple myeloma; AIDS associated leukemias and adult T-cell leukemia lymphoma; intraepithelial neoplasms, including Bowen's disease and Paget's disease; liver cancer (hepatocarcinoma); lung cancer; lymphomas, including Hodgkin's disease and lymphocytic lymphomas; neuroblastomas; oral cancer, including squamous cell carcinoma; ovarian cancer, including those arising from epithelial cells, stromal cells, germ cells and mesenchymal cells; pancreas cancer; prostate cancer; rectal cancer; sarcomas, including leiomyosarcoma, rhabdomyosarcoma, liposarcoma, fibrosarcoma and osteosarcoma; skin cancer, including melanoma, Kaposi's sarcoma, basocellular cancer and squamous cell cancer; testicular cancer, including germinal tumors (seminoma, non-seminoma [teratomas, choriocarcinomas]), stromal tumors and germ cell tumors; thyroid cancer, including thyroid adenocarcinoma and medullar carcinoma; and renal cancer including adenocarcinoma and Wilms tumor.
The invention also encompasses a method of contraception in a patient in need thereof comprising administering an inventive chemorepellant in an amount effective to inhibit migration of germ cells in the subject. According to another aspect of the invention, a method of treating infertililty and premature labor is provided. The method comprises administering a compound described above in an amount effective to inhibit immune cells from migrating close to a germ cell in the subject.
The treatment methods disclosed herein involve administering, either locally or systemically, to a selected site in a subject in need of such a treatment a chemorepellant of the invention in an amount effective to induce negative chemotaxis of a human migratory cell or an inhibitor of a chemorepellant in an amount effect to suppress negative chemotaxis of an immune cell. For example, a “therapeutically effective amount” in reference to the treatment of an inflammatory condition encompasses an amount sufficient to induce negative chemotaxis of an immune cell and/or ameliorate a symptom of the inflammatory condition.
In certain embodiments, the chemorepellant can be co-administered with a second agent (e.g., another chemoattractant or with any drug or agent which is not itself a chemoattractant). Co-administered agents, compounds, chemoattractants or therapeutics need not be administered at exactly the same time. In certain embodiments, however, the chemorepellant is administered substantially simultaneously as the second agent. By “substantially simultaneously,” it is meant that the chemorepellant is administered before, at the same time, and/or after the administration of the second agent. Second agents include, for example, anti-inflammatory agents, anti-cancer agents, anti-infective agents, immune therapeutics (immunosuppresants) and other therapeutic compounds. A second agent can be chosen based on the condition or disease to be treated. For example, in a method of treating cancer or a tumor, the chemorepellant can be administered with an anti-cancer agent. Similarly, in a method of treating an inflammatory condition, the chemorepellant can be administered with an anti-inflammatory agent, an anti-infective agent or an immunosuppressant.
An anti-infective agent is an agent which reduces the activity of or kills a microorganism and includes: Aztreonam; Chlorhexidine Gluconate; Imidurea; Lycetamine; Nibroxane; Pirazmonam Sodium; Propionic Acid; Pyrithione Sodium; Sanguinarium Chloride; Tigemonam Dicholine; Acedapsone; Acetosulfone Sodium; Alamecin; Alexidine; Amdinocillin; Amdinocillin Pivoxil; Amicycline; Amifloxacin; Amifloxacin Mesylate; Amikacin; Amikacin Sulfate; Aminosalicylic acid; Aminosalicylate sodium; Amoxicillin; Amphomycin; Ampicillin; Ampicillin Sodium; Apalcillin Sodium; Apramycin; Aspartocin; Astromicin Sulfate; Avilamycin; Avoparcin; Azithromycin; Azlocillin; Azlocillin Sodium; Bacampicillin Hydrochloride; Bacitracin; Bacitracin Methylene Disalicylate; Bacitracin Zinc; Bambermycins; Benzoylpas Calcium; Berythromycin; Betamicin Sulfate; Biapenem; Biniramycin; Biphenamine Hydrochloride; Bispyrithione Magsulfex; Butikacin; Butirosin Sulfate; Capreomycin Sulfate; Carbadox; Carbenicillin Disodium; Carbenicillin Indanyl Sodium; Carbenicillin Phenyl Sodium; Carbenicillin Potassium; Carumonam Sodium; Cefaclor; Cefadroxil; Cefamandole; Cefamandole Nafate; Cefamandole Sodium; Cefaparole; Cefatrizine; Cefazaflur Sodium; Cefazolin; Cefazolin Sodium; Cefbuperazone; Cefdinir; Cefepime; Cefepime Hydrochloride; Cefetecol; Cefixime; Cefinenoxime Hydrochloride; Cefmetazole; Cefmetazole Sodium; Cefonicid Monosodium; Cefonicid Sodium; Cefoperazone Sodium; Ceforanide; Cefotaxime Sodium; Cefotetan; Cefotetan Disodium; Cefotiam Hydrochloride; Cefoxitin; Cefoxitin Sodium; Cefpimizole; Cefpimizole Sodium; Cefpiramide; Cefpiramide Sodium; Cefpirome Sulfate; Cefpodoxime Proxetil; Cefprozil; Cefroxadine; Cefsulodin Sodium; Ceftazidime; Ceftibuten; Ceftizoxime Sodium; Ceftriaxone Sodium; Cefuroxime; Cefuroxime Axetil; Cefuroxime Pivoxetil; Cefuroxime Sodium; Cephacetrile Sodium; Cephalexin; Cephalexin Hydrochloride; Cephaloglycin; Cephaloridine; Cephalothin Sodium; Cephapirin Sodium; Cephradine; Cetocycline Hydrochloride; Cetophenicol; Chloramphenicol; Chloramphenicol Palmitate; Chloramphenicol Pantothenate Complex; Chloramphenicol Sodium Succinate; Chlorhexidine Phosphanilate; Chloroxylenol; Chlortetracycline Bisulfate; Chlortetracycline Hydrochloride; Cinoxacin; Ciprofloxacin; Ciprofloxacin Hydrochloride; Cirolemycin; Clarithromycin; Clinafloxacin Hydrochloride; Clindamycin; Clindamycin Hydrochloride; Clindamycin Palmitate Hydrochloride; Clindamycin Phosphate; Clofazimine; Cloxacillin Benzathine; Cloxacillin Sodium; Cloxyquin; Colistimethate Sodium; Colistin Sulfate; Coumermycin; Coumermycin Sodium; Cyclacillin; Cycloserine; Dalfopristin; Dapsone; Daptomycin; Demeclocycline; Demeclocycline Hydrochloride; Demecycline; Denofungin; Diaveridine; Dicloxacillin; Dicloxacillin Sodium; Dihydrostreptomycin Sulfate; Dipyrithione; Dirithromycin; Doxycycline; Doxycycline Calcium; Doxycycline Fosfatex; Doxycycline Hyclate; Droxacin Sodium; Enoxacin; Epicillin; Epitetracycline Hydrochloride; Erythromycin; Erythromycin Acistrate; Erythromycin Estolate; Erythromycin Ethylsuccinate; Erythromycin Gluceptate; Erythromycin Lactobionate; Erythromycin Propionate; Erythromycin Stearate; Ethambutol Hydrochloride; Ethionamide; Fleroxacin; Floxacillin; Fludalanine; Flumequine; Fosfomycin; Fosfomycin Tromethamine; Fumoxicillin; Furazolium Chloride; Furazolium Tartrate; Fusidate Sodium; Fusidic Acid; Gentamicin Sulfate; Gloximonam; Gramicidin; Haloprogin; Hetacillin; Hetacillin Potassium; Hexedine; Ibafloxacin; Imipenem; Isoconazole; Isepamicin; Isoniazid; Josamycin; Kanamycin Sulfate; Kitasamycin; Levofuraltadone; Levopropylcillin Potassium; Lexithromycin; Lincomycin; Lincomycin Hydrochloride; Lomefloxacin; Lomefloxacin Hydrochloride; Lomefloxacin Mesylate; Loracarbef; Mafenide; Meclocycline; Meclocycline Sulfosalicylate; Megalomicin Potassium Phosphate; Mequidox; Meropenem; Methacycline; Methacycline Hydrochloride; Methenamine; Methenamine Hippurate; Methenamine Mandelate; Methicillin Sodium; Metioprim; Metronidazole Hydrochloride; Metronidazole Phosphate; Mezlocillin; Mezlocillin Sodium; Minocycline; Minocycline Hydrochloride; Mirincamycin lydrochloride; Monensin; Monensin Sodium; Nafcillin Sodium; Nalidixate Sodium; Nalidixic Acid; Natamycin; Nebramycin; Neomycin Palmitate; Neomycin Sulfate; Neomycin Undecylenate; Netilmicin Sulfate; Neutramycin; Nifuradene; Nifuraldezone; Nifuratel; Nifuratrone; Nifurdazil; Nifurimide; Nifurpirinol; Nifurquinazol; Nifurthiazole; Nitrocycline; Nitrofurantoin; Nitromide; Norfloxacin; Novobiocin Sodium; Ofloxacin; Ormetoprim; Oxacillin Sodium; Oximonam; Oximonam Sodium; Oxolinic Acid; Oxytetracycline; Oxytetracycline Calcium; Oxytetracycline Hydrochloride; Paldimycin; Parachlorophenol; Paulomycin; Pefloxacin; Pefloxacin Mesylate; Penamecillin; Penicillin G Benzathine; Penicillin G Potassium; Penicillin G Procaine; Penicillin G Sodium; Penicillin V; Penicillin V Benzathine; Penicillin V Hydrabamine; Penicillin V Potassium; Pentizidone Sodium; Phenyl Aminosalicylate; Piperacillin Sodium; Pirbenicillin Sodium; Piridicillin Sodium; Pirlimycin Hydrochloride; Pivampicillin Hydrochloride; Pivampicillin Pamoate; Pivampicillin Probenate; Polymyxin B Sulfate; Porfiromycin; Propikacin; Pyrazinamide; Pyrithione Zinc; Quindecamine Acetate; Quinupristin; Racephenicol; Ramoplanin; Ranimycin; Relomycin; Repromicin; Rifabutin; Rifametane; Rifamexil; Rifamide; Rifampin; Rifapentine; Rifaximin; Rolitetracycline; Rolitetracycline Nitrate; Rosaramicin; Rosaramicin Butyrate; Rosaramicin Propionate; Rosaramicin Sodium Phosphate; Rosaramicin Stearate; Rosoxacil; Roxarsone; Roxithromycin; Sancycline; Sanfetrinem Sodium; Sarmoxicillin; Sarpicillin; Scopafungin; Sisomicin; Sisomicin Sulfate; Sparfloxacin; Spectinomycin Hydrochloride; Spiramycin; Stallimycin Hydrochloride; Steffimycin; Streptomycin Sulfate; Streptonicozid; Sulfabenz: Sulfabenzamide; Sulfacetamide; Sulfacetamide Sodium; Sulfacytine; Sulfadiazine; Sulfadiazine Sodium; Sulfadoxine; Sulfalene; Sulfamerazine; Sulfameter; Sulfamethazine; Sulfamethizole; Sulfamethoxazole; Sulfamonomethoxine; Sulfamoxole; Sulfanilate Zinc; Sulfanitran; Sulfasalazine; Sulfasomizole; Sulfathiazole; Sulfazamet; Sulfisoxazole; Sulfisoxazole Acetyl; Sulfisoxazole Diolamine; Sulfomyxin; Sulopenem; Sultamicillin; Suncillin Sodium; Talampicillin Hydrochloride; Teicoplanin; Temafloxacin Hydrochloride; Temocillin; Tetracycline; Tetracycline Hydrochloride; Tetracycline Phosphate Complex; Tetroxoprim; Thiamphenicol; Thiphencillin Potassium; Ticarcillin Cresyl Sodium; Ticarcillin Disodium; Ticarcillin Monosodium; Ticlatone; Tiodonium Chloride; Tobramycin; Tobramycin Sulfate; Tosufloxacin; Trimethoprim; Trimethoprim Sulfate; Trisulfapyrimidines; Troleandomycin; Trospectomycin Sulfate; Tyrothricin; Vancomycin; Vancomycin Hydrochloride; Virginiamycin; Zorbamycin; Difloxacin Hydrochloride; Lauryl Isoquinolinium Bromide; Moxalactam Disodium; Ornidazole; Pentisomicin; and Sarafloxacin Hydrochloride.
Exemplary anti-cancer agents include Acivicin; Aclarubicin; Acodazole Hydrochloride; Acronine; Adozelesin; Aldesleukin; Altretamine; Ambomycin; Ametantrone Acetate; Aminoglutethimide; Amsacrine; Anastrozole; Anthramycin; Asparaginase; Asperlin; Azacitidine; Azetepa; Azotomycin; Batimastat; Benzodepa; Bicalutamide; Bisantrene Hydrochloride; Bisnafide Dimesylate; Bizelesin; Bleomycin Sulfate; Brequinar Sodium; Bropirimine; Busulfan; Cactinomycin; Calusterone; Caracemide; Carbetimer; Carboplatin; Carmustine; Carubicin Hydrochloride; Carzelesin; Cedefingol; Chlorambucil; Cirolemycin; Cisplatin; Cladribine; Crisnatol Mesylate; Cyclophosphamide; Cytarabine; Dacarbazine; Dactinomycin; Daunorubicin Hydrochloride; Decitabine; Dexormaplatin; Dezaguanine; Dezaguanine Mesylate; Diaziquone; Docetaxel; Doxorubicin; Doxorubicin Hydrochloride; Droloxifene; Droloxifene Citrate; Dromostanolone Propionate; Duazomycin; Edatrexatc; Eflorithine Hydrochloride; Elsamitrucin; Enloplatin; Enpromate; Epipropidine; Epirubicin Hydrochloride; Erbulozole; Esorubicin Hydrochloride; Estramustine; Estramustine Phosphate Sodium; Etanidazole; Etoposide; Etoposide Phosphate; Etoprine; Fadrozole Hydrochloride; Fazarabine; Fenretinide; Floxuridine; Fludarabine Phosphate; Fluorouracil; Flurocitabine; Fosquidone; Fostriecin Sodium; Gemcitabine; Gemcitabine Hydrochloride; Hydroxyurea; Idarubicin Hydrochloride; Ifosfamide; Ilmofosine; Interferon Alfa-2a; Interferon Alfa-2b; Interferon Alfa-n1; Interferon Alfa-n3; Interferon Beta-I a; Interferon Gamma-I b; Iproplatini; Irinotecan Hydrochloride; Lanreotide Acetate; Letrozole; Leuprolide Acetate; Liarozole Hydrochloride; Lometrexol Sodium; Lomustine; Losoxantrone Hydrochloride; Masoprocol; Maytansine; Mechlorethamine Hydrochloride; Megestrol Acetate; Melengestrol Acetate; Melphalan; Menogaril; Mercaptopurine; Methotrexate; Methotrexate Sodium; Metoprine; Meturedepa; Mitindomide; Mitocarcin; Mitocromin; Mitogillin; Mitomalcin; Mitomycin; Mitosper; Mitotane; Mitoxantrone Hydrochloride; Mycophenolic Acid; Nocodazole; Nogalamycin; Ormaplatin; Oxisuran; Paclitaxel; Pegaspargase; Peliomycin; Pentamustine; Peplomycin Sulfate; Perfosfamide; Pipobroman; Piposulfan; Piroxantrone Hydrochloride; Plicamycin; Plomestane; Podofilox; Porfimer Sodium; Porfiromycin; Prednimustine; Procarbazine Hydrochloride; Puromycin; Puromycin Hydrochloride; Pyrazofurin; Riboprine; Rogletimide; Safingol; Safingol Hydrochloride; Semustine; Simtrazene; Sparfosate Sodium; Sparsomycin; Spirogermanium Hydrochloride; Spiromustine; Spiroplatin; Streptonigrin; Streptozocin; Sulofenur; Talisomycin; Taxotere; Tecogalan Sodium; Tegafur; Teloxantrone Hydrochloride; Temoporfin; Teniposide; Teroxirone; Testolactone; Thiamiprine; Thioguanine; Thiotepa; Tiazofurin; Tirapazamine; Topotecan Hydrochloride; Toremifene Citrate; Trestolone Acetate; Triciribine Phosphate; Trimetrexate; Trimetrexate Glucuronate; Triptorelin; Tubulozole Hydrochloride; Uracil Mustard; Uredepa; Vapreotide; Verteporlin; Vinblastine Sulfate; Vincristine Sulfate; Vindesine; Vindesine Sulfate; Vinepidine Sulfate; Vinglycinate Sulfate; Vinleurosine Sulfate; Vinorelbine Tartrate Virlrosidine Sulfate; Vinzolidine Sulfate; Vorozole; Zeniplatin; Zinostatin; and Zorubicin Hydrochloride. Exemplary immunosuppressants include Azathioprine; Azathioprine Sodium; Cyclosporine; Daltroban; Gusperimus Trihydrochloride; Sirolimus; and Tacrolimus. Exemplary anti-inflammatory agents include Alclofenac; Alclometasone Dipropionate; Algestone Acetonide; Alpha Amylase; Amcinafal; Amcinafide; Amfenac Sodium; Amiprilose Hydrochloride; Anakinra; Anirolac; Anitrazafen; Apazone; Balsalazide Disodium; Bendazac; Benoxaprofen; Benzydamine Hydrochloride; Bromelains; Broperamole; Budesonide; Carprofen; Cicloprofen; Cintazone; Cliprofen; Clobetasol Propionate; Clobetasone Butyrate; Clopirac; Cloticasone Propionate; Cormethasone Acetate; Cortodoxone; Deflazacort; Desonide; Desoximetasone; Dexamethasone Dipropionate; Diclofenac Potassium; Diclofenac Sodium; Diflorasone Diacetate; Diflumidone Sodium; Diflunisal; Difluprednate; Diftalone; Dimethyl Sulfoxide; Drocinonide; Endrysone; Enlimomab; Enolicam Sodium; Epirizole; Etodolac; Etofenamate; Felbinac; Fenamole; Fenbufen; Fenclofenac; Fenclorac; Fendosal; Fenpipalone; Fentiazac; Flazalone; Fluazacort; Flufenamic Acid; Flumizole; Flunisolide Acetate; Flunixin; Flunixin Meglumine; Fluocortin Butyl; Fluorometholone Acetate; Fluquazone; Flurbiprofen; Fluretofen; Fluticasone Propionate; Furaprofen; Furobufen; Halcinonide; Halobetasol Propionate; Halopredone Acetate; Ibufenac; Ibuprofen; Ibuprofen Aluminum; Ibuprofen Piconol; Ilonidap; Indomethacin; Indomethacin Sodium; Indoprofen; Indoxole; Intrazole; Isoflupredone Acetate; Isoxepac; Isoxicam; Ketoprofen; Lofemizole Hydrochloride; Lornoxicam; Loteprednol Etabonate; Meclofenamate Sodium; Meclofenamic Acid; Meclorisone Dibutyrate; Mefenamic Acid; Mesalamine; Meseclazone; Methylprednisolone Suleptanate; Morniflumate; Nabumetone; Naproxen; Naproxen Sodium; Naproxol; Nimazone; Olsalazine Sodium; Orgotein; Orpanoxin; Oxaprozin; Oxyphenbutazone; Paranyline Hydrochloride; Pentosan Polysulfate Sodium; Phenbutazone Sodium Glycerate; Pirfenidone; Piroxicam; Piroxicam Cinnamate; Piroxicam Olamine; Pirprofen; Prednazate; Prifelone; Prodolic Acid; Proquazone; Proxazole; Proxazole Citrate; Rimexolone; Romazarit; Salcolex; Salnacedin; Salsalate; Sanguinarium Chloride; Seclazone; Sermetacin; Sudoxicam; Sulindac; Suprofen; Talmetacin; Talniflumate; Talosalate; Tebufelone; Tenidap; Tenidap Sodium; Tenoxicam; Tesicam; Tesimide; Tetrydamine; Tiopinac; Tixocortol Pivalate; Tolmetin; Tolmetin Sodium; Triclonide; Triflumidate; Zidometacin; and Zomepirac Sodium.
As used herein, “treatment” and/or “treating” refer to therapeutic treatment as well as prophylactic treatment or preventative measures. The chemorepellant and/or other therapeutic (such as an antibody to the chemorepellant) can be administered in pharmaceutical compositions comprising a pharmaceutically acceptable carrier or excipient. The excipient can be chosen based on the expected route of administration of the composition in therapeutic applications. The route of administration of the composition depends on the condition to be treated. Routes of administration include, but are not limited to, parenteral, topic, oral, intramuscular, intravenous administration. The route of administration and the dosage of the composition to be administered can be determined by the skilled artisan without undue experimentation in conjunction with standard dose-response studies. Relevant circumstances to be considered in making those determinations include the condition or conditions to be treated, the choice of composition to be administered, the age, weight, and response of the individual patient, and the severity of the patient's symptoms. In one embodiment, the chemorepellant or a composition thereof is administered locally.
The therapeutic compositions used in the inventive methods can be administered parenterally such as, for example, by intravenous, intramuscular, intrathecal, or subcutaneous injection. Parenteral administration can be accomplished by incorporating the therapeutic compositions of the present invention into a solution or suspension. Such solutions or suspensions may also include sterile diluents such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol, or other synthetic solvents. Parenteral formulations may also include antibacterial agents such as, for example, benzyl alcohol or methyl parabens, antioxidants such as, for example, ascorbic acid or sodium bisulfite and chelating agents such as EDTA. Buffers such as acetates, citrates or phosphates and agents for the adjustment of tonicity such as sodium chloride or dextrose may also be added. The parenteral preparation can be enclosed in ampules, disposable syringes, or multiple dose vials made of glass or plastic.
The invention is illustrated by the following examples which are not meant to be limiting in any way.
Objective: To identify the agents present in tumor microenvironments that have the ability to modulate the migration of immune cell subsets.
Materials and Methods:
Cystic fluid samples: Fluids from ovarian carcinoma patients were collected during surgical procedures under a signed informed consent. Fluids were centrifuged to remove the debris. The supernatants were supplemented with cocktail of protease inhibitors and divided into aliquots and stored at −80 C till further processing. Samples were evaluated to study their effects on migration of neutrophils in transwell migration assays in Boyden chambers for their chemoattraction (CA) and chemorepulsion (CR) activities as described below.
Chromatographic separation: Cystic fluid (0.2 ml at 65 mg/ml) was loaded on a Superdex 200 10/300 GL column (GE Healthcare) and fractionated at the rate of 0.5 ml/min. Fractions (1 ml) were collected in tubes preloaded with 10 μl of 100× concentration Complete EDTA-free Protease Inhibitor Cocktail (Roche). These fractions were evaluated for CA CR activities in transwell migration assays described below.
One and two dimensional SDS-PAGE analysis: Fractions collected from S-200 chromatography with CR activity and the adjacent fractions without CR activity were further fractionated by one and two dimensional SDS-PAGE. Proteins band and/or spots differentially present in S-200 fractions with CR activity were excised manually, digested with trypsin, and subjected to either LC-MS/MS (1-D bands) or MALDI (2-D spots) analysis.
The chemorepulsive activity of the cystic fluid, fractions collected from S-200 chromatography and the proteins listed below was determined as follows:
Prior to beginning the assay, the following were prepared:
The plate was covered with the supplied lid and incubated for the desired time at 37° C. in 5% CO2. Unless otherwise indicated, the incubation time was 1 hour for neutrophils and 3 hours for T cells. For monocytes and B cells, the incubation time was 2 hours. After the desired assay time, the liquid was removed from the top of the plate using a Kimwipe.
The membrane was carefully removed from the top of the plate and discarded. The plate was examined under a microscope to look for ligand crystallization, contamination and overall migration.
From mass spectrometry (MS) analysis, 86 proteins in the chemorepulsion active chromatography fraction have been identified which are represented in the following table.
Some of these proteins were evaluated individually and in combinations for their effects on CA and CR activity. Of these proteins, actin, 14-3-3 zeta/delta, apolipoprotein A1 and hemopexin showed the greatest CA and/or CR activities.
Legends for the Figures:
Objective: To identify the agents present in mammalian cancer cell lines that have the ability to modulate the migration of immune cell subsets.
Materials and Methods:
Mammalian Cancer Cell Lines:
Cancer cell lines were cultured in serum containing media until desired confluence is reached. Culture conditions were switched to serum-free media and supernatants collected everyday up to certain number of days. The supernatants were supplemented with cocktail of protease inhibitors and divided into aliquots and stored at −80 C until further processing. Depending on the volume of culture supernatant, they were either concentrated 10 times or evaluated unconcentrated to study their effects on neutrophil migration Boyden chamber transwell migration assays.
Chromatographic Separation:
Supernatants were further concentrated and loaded on a Superdex 200 10/300 GL column (GE Healthcare) and fractionated at the rate of 0.5 ml/min. Fractions (1 ml) were collected in tubes preloaded with 10 ul of 100× concentration Complete EDTA-free Protease Inhibitor Cocktail (Roche). These fractions were evaluated for chemoattraction (CA) and chemorepulsion (CR) activities in transwell migration assays described below.
Supernatants for the breast cancer cell line, SK-BR-3 were first dialyzed overnight and then loaded on a HiTrap-Q Fast Flow anion exchange column and fractionated at a rate of 1 mL/min. 3 mL fractions were desalted and evaluated for chemoattraction (CA) and chemorepulsion (CR) activities in transwell migration assays as described below.
One Dimensional SDS-PAGE Analysis:
Fractions collected from S-200 and anion exchange chromatography with CR activity and the adjacent fractions without CR activity were further fractionated by one dimensional SDS-PAGE. Proteins bands differentially present in S-200 fractions with CR activity were excised manually, digested with trypsin, and subjected to LC-MS/MS.
The chemorepulsive activity of the supernatants, fractions collected from S-200 and anion exchange chromatography and the proteins listed below were determined as follows:
Transwell Migration Assay:
Bands from supernatant fractions that exhibited chemorepulsive activity were sent out for MS (Liquid chromatography/Mass Spectrometry/Mass Spectrometry) analysis (outsourced). Commercially available proteins corresponding to proteins identified in Mass Spectrometry were then tested in cell migration assay.
Protein identification was performed by outside sources using nano LC/MS/MS (Liquid Chromatography/Mass Spectrometry/Mass Spectrometry) on an LTQ (“linear trap quadrupole”) mass spectrometer. Protein samples were submitted in a gel or solution and were first digested robotically using trypsin to create a peptide mixture (alternate enzymes may be employed if necessary). Peptides were then injected on a custom-designed LC column set-up and eluted into the mass spectrometer where MS and MS/MS were performed. Product ion data was searched using forward and reversed database searching methods to allow assessment of false discovery rates and ensure only correct protein identifications were reported. Search results were parsed into the Scaffold™ visualization software to allow further validation of protein assignments through the ProteinProphet™ and PeptideProphet™1 tools.
The methods used for In-gel digestion are as below:
Samples were subjected to proteolytic digestion on a ProGest workstation as follows:
The samples were reduced with DTT at 60° C., allowed to cool to room temperature, alkylated with iodoacetamide, incubated at 37° C. for 4 h in the presence trypsin and formic acid was added to stop the reaction.
The method used for Mass Spectrometry—Solution Based are below:
Samples were subjected to C18 capture using ZipTips. They were aspirated across equilibrated C18 ZipTip, washed in 0.1% formic acid, eluted in 80% acetonitrile in 0.1% formic acid, concentrated by vacuum centrifugation and resuspended in 0.1% formic acid for injection.
The methods used for LC/MS/MS (data-dependent) are as below:
NOTE: Detailed protocols for each of these methods can be found in the technical information section of http://www.prsproteomics.com.
NOTE: SK-BR-3 was outsourced using LC/MS/MS performed at University of Georgia, Proteomics Resource Facility.
Results:
The chemorepulsive activity of supernatants, fractions collected from chromatography and commercially available proteins are shown in
Proteins identified in the chemorepulsive supernatant fractions by LC/MS/MS (mass spectrometry) are shown in the Tables below:
As shown in the figures, the following proteins were identified in chemorepulsive fractions of supernatants from cell lines and/or ovarian cystic fluid were shown to induce negative chemotaxis of neutrophils:
actin, 14-3-3 zeta/delta, apolipoprotein A1, hemopexin, PARK7, cofilin-1, 14-3-3 epsilon, 14-3-3-gamma, phosphoserine phosphatase, superoxide dismutase, profilin-1, beta-2 microglobulin, cytochrome c, cystatin B, macrophage migration inhibitory factor (MIF), FK506 binding protein, thioredoxin, galectin 3, human transferrin, human EF-1-gamma and human galectin 3 binding protein.
Profilin-1 was identified in chemorepulsive supernatant fractions. As shown in the figures, profilin-2 was shown to induce negative chemotaxis.
Table 10 shows chemorepellant proteins that were isolated from chemorepellant fractions of at least two cells or from a cell line and ovarian cystic fluid (as indicated by an “X”) and were shown to induce chemorepulsion of neutrophils in their purified form (as described in Examples 1 and 2). For example, Actin was identified in the chemorepulsive fractions isolated from the supernatant of SF-539 cells and from ovarian cystic fluid sample (described in Example 1).
Table 11 lists proteins identified in chemorepellant fractions of at least two cell lines or at least one cell line and ovarian cyst fluid.
While this invention has been particularly shown and described with references to preferred embodiments thereof, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention encompassed by the appended claims.
This application claims the benefit of U.S. Provisional Application No. 61/102,177, filed Oct. 2, 2008 and U.S. Provisional Application No. 61/222,217 filed Jul. 1, 2009. The entire teachings of the above applications are incorporated herein by reference.
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