NUCLEIC ACID PRODUCTS AND METHODS OF ADMINISTRATION THEREOF

FIELD OF THE INVENTION

The present invention relates, in part, to methods, compositions, and products for producing and delivering nucleic acids to cells, tissues, organs, and patients, methods for expressing proteins in cells, tissues, organs, and patients, and cells, therapeutics, and cosmetics produced using these methods, compositions, and products.

DESCRIPTION OF THE TEXT FILE SUBMITTED ELECTRONICALLY

The instant application contains a Sequence Listing which has been submitted electronically in XML format and is hereby incorporated by reference in its entirety. Said XML copy, created on Aug. 17, 2022, is named 61057-701310-SL.xml and is 904,648 bytes in size.

BACKGROUND
Synthetic RNA and Nucleic-Acid Therapeutics

Ribonucleic acid (RNA) is ubiquitous in both prokaryotic and eukaryotic cells, where it encodes genetic information in the form of messenger RNA, binds and transports amino acids in the form of transfer RNA, assembles amino acids into proteins in the form of ribosomal RNA, and performs numerous other functions including gene expression regulation in the forms of microRNA and long non-coding RNA. RNA can be produced synthetically by methods including direct chemical synthesis and in vitro transcription, and can be administered to patients for therapeutic use. However, previously described synthetic RNA molecules are unstable and trigger a potent innate-immune response in human cells. In addition, methods for efficient non-viral delivery of nucleic acids to patients, organs, tissues, and cells in vivo have not been previously described. The many drawbacks of existing synthetic RNA technologies and methods for delivery of nucleic acids make them undesirable for therapeutic or cosmetic use.

Cell Reprogramming and Cell-Based Therapies

Cells can be reprogrammed by exposing them to specific extracellular cues and/or by ectopic expression of specific proteins, microRNAs, etc. While several reprogramming methods have been previously described, most that rely on ectopic expression require the introduction of exogenous DNA, which can carry mutation risks. DNA-free reprogramming methods based on direct delivery of reprogramming proteins have been reported. However, these methods are too inefficient and unreliable for commercial use. In addition, RNA-based reprogramming methods have been described (see, e.g., Angel. MIT Thesis. 2008. 1-56; Angel et al. PLoS ONE. 2010. 5, 107; Warren et al. Cell Stem Cell. 2010. 7, 618-630; Angel. MIT Thesis. 2011. 1-89; and Lee et al. Cell. 2012. 151, 547-558; the contents of all of which are hereby incorporated by reference). However, existing RNA-based reprogramming methods are slow, unreliable, and inefficient when performed on adult cells, require many transfections (resulting in significant expense and opportunity for error), can reprogram only a limited number of cell types, can reprogram cells to only a limited number of cell types, require the use of immunosuppressants, and require the use of multiple human-derived components, including blood-derived HSA and human fibroblast feeders. The many drawbacks of previously disclosed RNA-based reprogramming methods make them undesirable for research, therapeutic or cosmetic use.

Gene Editing

Several naturally occurring proteins contain DNA-binding domains that can recognize specific DNA sequences, for example, zinc fingers (ZFs) and transcription activator-like effectors (TALEs). Fusion proteins containing one or more of these DNA-binding domains and the cleavage domain of FokI endonuclease can be used to create a double-strand break in a desired region of DNA in a cell (see, e.g., US Patent Appl. Pub. No. US 2012/0064620, US Patent Appl. Pub. No. US 2011/0239315, U.S. Pat. No. 8,470,973, US Patent Appl. Pub. No. US 2013/0217119, U.S. Pat. No. 8,420,782, US Patent Appl. Pub. No. US 2011/0301073, US Patent Appl. Pub. No. US 2011/0145940, U.S. Pat. Nos. 8,450,471, 8,440,431, 8,440,432, and US Patent Appl. Pub. No. 2013/0122581, the contents of all of which are hereby incorporated by reference). Other gene-editing proteins include clustered regularly interspaced short palindromic repeat (CRISPR)-associated proteins. However, current methods for gene editing cells are inefficient and carry a risk of uncontrolled mutagenesis, making them undesirable for research, therapeutic or cosmetic use. Methods for DNA-free gene editing of somatic cells have not been previously explored, nor have methods for simultaneous or sequential gene editing and reprogramming of somatic cells. In addition, methods for directly gene editing cells in patients (i.e., in vivo) have not been previously explored, and the development of such methods has been limited by a lack of acceptable targets, inefficient delivery, inefficient expression of the gene-editing protein/proteins, inefficient gene editing by the expressed gene-editing protein/proteins, due in part to poor binding of DNA-binding domains, excessive off-target effects, due in part to non-directed dimerization of the FokI cleavage domain and poor specificity of DNA-binding domains, and other factors. Finally, the use of gene editing in anti-bacterial, anti-viral, and anti-cancer treatments has not been previously explored.

Accordingly, there remains a need for improved methods and compositions for the production and delivery of nucleic acids to cells, tissues, organs, and patients.

SUMMARY OF THE INVENTION

The present invention provides, in part, compositions, methods, articles, and devices for delivering nucleic acids to cells, tissues, organs, and patients, methods for inducing cells to express proteins, methods, articles, and devices for producing these compositions, methods, articles, and devices, and compositions and articles, including cells, organisms, cosmetics and therapeutics, produced using these compositions, methods, articles, and devices. Unlike previously reported methods, certain embodiments of the present invention provide small doses of nucleic acids to achieve significant and lasting protein expression in humans.

In some aspects, the present invention relates to a method for treating a metabolic disorder. The method comprises a step of administering an effective amount of a synthetic RNA encoding growth differentiation factor 15 (GDF15) to a subject in need thereof or endothelial cell specific molecule 1 (ESM1), wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the metabolic disorder is selected from Type I diabetes, Type II diabetes, insulin resistance, obesity, dyslipidemia, hypercholesterolemia, hyperglycemia, hyperinsulinemia, hypertension, hepatosteaotosis such as non-alcoholic steatohepatitis (NASH) and non-alcoholic fatty acid liver disease (NAFLD), cancer, a disease or disorder associated with impaired lipid metabolism, a disease or disorder associated with impaired renal function, a disease or disorder associated with impaired hepatic function, a disease or disorder associated with impaired lung function, a vascular or cardiovascular disease or disorder, muscle wasting, inflammation, and a respiratory disease.

In various embodiments, the disease or disorder associated with impaired renal function is selected from chronic kidney diseases, acute kidney injury, nephropathy, diabetic nephropathy, kidney failure, and kidney fibrosis.

In various embodiments, the vascular or cardiovascular disease or disorder is selected from coronary artery disease, cardiomyopathy, hypertension, atrial fibrillation, preeclampsia, peripheral artery disease, atherosclerosis, heart failure, acute myocardial infarction, acute coronary syndrome, muscle wasting, hypertensive ventricular hypertrophy, hypertensive cardiomyopathy, ischemic heart disease, myocardial infarction, abdominal aortic aneurysm, a blood clot, deep vein thrombosis, venous stasis disease, phlebitis, and varicose veins.

In various embodiments, the treatment results in reduction in one or more of aspartate transaminase (AST), alanine transaminase (ALT), or glucose levels in the subject, as compared to untreated subjects.

In various embodiments, the treatment results in increased lipid mobilization in the subject, as compared to untreated subjects.

In various embodiments, the administration is intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, oral, sublingual, intracerebral, intravaginal, transdermal, intraportal, rectally, by inhalation, or topically.

In some aspects, the present invention relates to a method for modulating GDF15. The method comprises a step of administering an effective amount of a synthetic RNA encoding GDF15 to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the modulating results in reduction in one or more of ALT, AST, or glucose levels in the subject, as compared to untreated subjects.

In various embodiments, the modulating results in increased lipid mobilization in the subject, as compared to untreated subjects.

In various embodiments, the modulating results in an increase in the quantity of GDF15 in the subject.

In various embodiments, the modulating results in a decrease in the quantity of GDF15 in the subject.

In some aspects, the present invention relates to a method for modulating ESM1. The method comprises a step of administering an effective amount of a synthetic RNA encoding ESM1 to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the modulating results in one or more of reduced ALT, AST, or glucose levels in the subject, as compared to untreated subjects.

In various embodiments, the modulating results in increased lipid mobilization in the subject, as compared to untreated subjects.

In various embodiments, the modulating results in an increase in the quantity of ESM1 in the subject.

In various embodiments, the modulating results in a decrease in the quantity of ESM1 in the subject.

In some aspects, the present invention relates to a method for treating a liver disorder. The method comprises a step of administering an effective amount of a synthetic RNA encoding growth differentiation factor 15 (GDF15) or endothelial cell specific molecule 1 (ESM1) to a subject in need thereof, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity; and the treatment reduces one or more of fatty liver, NAFLD, NASH, inflammation, hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma in the subject.

In various embodiments, the treatment results in reduction in one or more of AST, ALT, or glucose levels in the subject, as compared to untreated subjects.

In various embodiments, the treatment results in increased lipid mobilization in the subject, as compared to untreated subjects.

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In some aspects, the present invention relates to a method for treating Friedrich's Ataxia. The method comprises a step of administering to a subject in need thereof an effective amount of (a) a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in FXNA (SEQ ID NO: 582), and/or (b) a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in FXNB (SEQ ID NO: 583), wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity. In various embodiments, the treatment comprises administering to a subject in need thereof an effective amount of (a) the synthetic RNA encoding the gene-editing protein capable of creating a double strand break in FXNA (SEQ ID NO: 582), and (b) the synthetic RNA encoding the gene-editing protein capable of creating a double strand break in FXNB (SEQ ID NO: 583).

In various embodiments, the administration is directed to the heart.

In various embodiments, the administration is via a catheter.

In various embodiments, the treatment ameliorates one or more of muscle weakness, loss of coordination, vision impairment, hearing impairment, slurred speech, scoliosis, pes cavus deformity of the foot, diabetes, and heart disorders, selected from one or more atrial fibrillation, tachycardia and hypertrophic cardiomyopathy.

In various embodiments, the gene-editing protein is selected from a CRISPR/Cas9, TALEN and a zinc finger nuclease.

In various embodiments, the gene-editing protein comprises: (a) a DNA-binding domain and (b) a nuclease domain. The DNA-binding domain comprises a plurality of repeat sequences and at least one of the repeat sequences comprises the amino acid sequence: LTPvQWAIAwxyzGHGG (SEQ ID NO: 629) and is between 36 and 39 amino acids long, wherein: “v” is Q, D or E; “w” is S or N; “x” is H, N, or I; “y” is D, A, I, N, G, H, K, S, or null; and “z” is GGKQALETVQRLLPVLCQD (SEQ ID NO: 630) or GGKQALETVQRLLPVLCQA (SEQ ID NO: 631). The nuclease domain comprises a catalytic domain of a nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In some aspects, the present invention relates to a method for reducing inflammation. The method comprises a step of administering an effective amount of a synthetic RNA encoding superoxide dismutase 3 (SOD3) or IFKB, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity to a subject in need thereof.

In various embodiments, the inflammation is associated with a lung disease or disorder.

In various embodiments, the lung disease or disorder is selected from Asbestosis, Asthma, Bronchiectasis, Bronchitis, Chronic Cough, Chronic Obstructive Pulmonary Disease (COPD), Common Cold, Croup, Cystic Fibrosis, Hantavirus, Idiopathic Pulmonary Fibrosis, Influenza, Lung Cancer, Pandemic Flu, Pertussis, Pleurisy, Pneumonia, Pulmonary Embolism, Pulmonary Hypertension, Respiratory Syncytial Virus (RSV), Sarcoidosis, Sleep Apnea, Spirometry, Sudden Infant Death Syndrome (SIDS), and Tuberculosis.

In various embodiments, the inflammation is associated with a lung infection.

In various embodiments, the lung infection is cause by a bacterium, a fungus, a protozoa, a multi-cellular organism, a particulate, or a virus.

In various embodiments, the synthetic RNA is administered by inhalation.

In various embodiments, the inflammation is associated with sepsis.

In some aspects, the present invention relates to a method for treating alpha-1 antitrypsin (A1AT) deficiency. The method comprises a step of administering to a subject in need thereof an effective amount of (a) a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in A1AT_A (SEQ ID NO: 584) and/or (b) a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in A1AT_B (SEQ ID NO: 585), wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity. In various embodiments, the treatment comprises administering to a subject in need thereof an effective amount of (a) the synthetic RNA encoding the gene-editing protein capable of creating a double strand break in A1AT_A (SEQ ID NO: 584) and (b) the synthetic RNA encoding the a gene-editing protein capable of creating a double strand break in A1AT_B (SEQ ID NO: 585).

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In various embodiments, the treatment corrects the Z mutation in the subject's liver cells.

In various embodiments, the treatment reduces polymerized Z protein accumulation in the subject's liver cells.

In various embodiments, the treatment increases secretion and/or serum levels of functional A1AT.

In various embodiments, the treatment ameliorates one or more of chronic cough, emphysema, COPD, liver failure, hepatitis, hepatomegaly, jaundice, and cirrhosis.

In various embodiments, the gene-editing protein is selected from a CRISPR/Cas9, TALEN, and a zinc finger nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In some aspects, the present invention relates to a method for treating alpha-1 antitrypsin (A1AT) deficiency. The method comprises a step of administering an effective amount of a synthetic RNA encoding A1AT to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In various embodiments, the treatment corrects the Z mutation in the subject's liver cells.

In various embodiments, the treatment reduces polymerized Z protein accumulation in the subject's liver cells.

In various embodiments, the treatment increases secretion and/or serum levels of functional A1AT.

In various embodiments, the treatment ameliorates one or more of the subject's symptoms.

In various embodiments, the method further comprises steps of (c) obtaining the cell contacted in step (b); and (d) administering the cell to a subject in need thereof.

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In some aspects, the present invention relates to a method for reversing an alpha-1 antitrypsin (A1AT) deficiency in a cell. The method comprises steps of (a) obtaining a cell comprising a defective A1AT gene and (b) contacting the cell with one or both of a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in A1AT_A (SEQ ID NO: 584) and a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in A1AT_B (SEQ ID NO: 585), wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the method further comprises steps of (c) obtaining the cell contacted in step (b); and (d) administering the cell to a subject in need thereof.

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In various embodiments, the gene-editing protein is selected from a CRISPR/Cas9, TALEN and a zinc finger nuclease.

In various embodiments, the gene-editing protein comprises: (a) a DNA-binding domain and (b) a nuclease domain.

The DNA-binding domain comprises a plurality of repeat sequences and at least one of the repeat sequences comprises the amino acid sequence: LTPvQWAIAwxyzGHGG (SEQ ID NO: 629) and is between 36 and 39 amino acids long, wherein: “v” is Q, D or E; “w” is S or N; “x” is H, N, or I; “y” is D, A, I, N, G, H, K, S, or null; and “z” is GGKQALETVQRLLPVLCQD (SEQ ID NO: 630) or GGKQALETVQRLLPVLCQA (SEQ ID NO: 631). The nuclease domain comprises a catalytic domain of a nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In some aspects, the present invention relates to a method for modulating BMP7. The method comprises a step of administering an effective amount of a synthetic RNA encoding BMP7 to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the modulating results in an increase in the quantity of BMP7 in the subject.

In various embodiments, the modulating results in a decrease in the quantity of BMP7 in the subject.

In various embodiments, the modulating results in an increase in intracellular alkaline phosphatase activity.

In various embodiments, the synthetic RNA encoding BMP7 comprises a sequence encoding the BMP7 signal peptide.

In various embodiments, the BMP7 signal peptide comprises the sequence of SEQ ID NO: 597.

In various embodiments, the administration is directed to the liver.

In various embodiments, the administration is intraportal injection.

In various embodiments, the administration treats diabetic nephropathy.

In various embodiments, the administration treats liver fibrosis.

In various embodiments, the administration is directed to the kidney.

In various embodiments, the administration is intravenous or intradermal.

In various embodiments, the administration treats kidney disease.

In various embodiments, the kidney disease is diabetic nephropathy.

In various embodiments, the modulating results in reduced levels of urine albumin in the subject.

In some aspects, the present invention relates to a method for modulating an immune checkpoint molecule. The method comprises a step of administering an effective amount of a synthetic RNA encoding the immune checkpoint molecule to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In some aspects, the present invention relates to a method for treating a subject comprising administering a step of a synthetic RNA encoding a gene-editing protein targeting an immune checkpoint molecule gene.

In some aspects, the present invention relates to a method for treating a subject comprising steps of (a) obtaining a cell comprising an immune checkpoint molecule gene and (b) contacting the cell with a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in the immune checkpoint molecule gene, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the method further comprises steps of (c) obtaining the cell contacted in step (b); and (d) administering the cell to a subject in need thereof.

In various embodiments, the gene-editing protein is selected from a CRISPR/Cas9, TALEN and a zinc finger nuclease.

“v” is Q, D or E; “w” is S or N; “x” is H, N, or I; “y” is D, A, I, N, G, H, K, S, or null; and “z” is GGKQALETVQRLLPVLCQD (SEQ ID NO: 630) or GGKQALETVQRLLPVLCQA (SEQ ID NO: 631). The nuclease domain comprises a catalytic domain of a nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In various embodiments, the immune checkpoint molecule is selected from PD-1, PD-L1, PD-L2, CTLA-4, ICOS, LAG3, OX40, OX40L, and TIM3.

In various embodiments, the immune checkpoint molecule is PD-1.

In various embodiments, the administering stimulates or enhances an immune response in the subject.

In various embodiments, the administering inhibits or reduces an immune response in the subject.

In various embodiments, the subject is afflicted with a cancer.

In various embodiments, the subject is afflicted with an autoimmune disease.

In various embodiments, the modulating results in an increase in the quantity of PD-1 in the subject.

In various embodiments, the modulating results in a decrease in the quantity of PD-1 in the subject.

In some aspects, the present invention relates to a method for treating a cancer comprising steps of (a) isolating an cell from a subject, the cell being an immune cell or hematopoietic cell; (b) contacting the isolated cell with an effective amount of a synthetic RNA encoding a chimeric antigen receptor (CAR), wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity; and (c) administering the cell to the subject.

In various embodiments, the immune cell is a T cell.

In some aspects, the present invention relates to a method for making a chimeric antigen receptor (CAR) T cell comprising steps of (a) obtaining a cell from a subject and (b) contacting the cell with a synthetic RNA encoding a gene-editing protein capable of creating a double strand break to yield a safe harbor locus for CAR insertion, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the wherein the gene-editing protein is selected from a CRISPR/Cas9, TALEN and a zinc finger nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In some aspects, the present invention relates to a method for increasing the persistence of a chimeric antigen receptor (CAR) T cell, comprising a step of contacting the chimeric antigen receptor (CAR) T cell with a synthetic RNA encoding a encoding a telomerase, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In some aspects, the present invention relates to a method for treating a skin wound, comprising a step of administering an effective amount of a synthetic RNA encoding interleukin 22 (IL22) to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the administration is directed to a population of cells of the integumentary system.

In various embodiments, the population of cells comprises one or more of cells of the epidermis, cells of the basement membrane, cells of the dermis, and/or cells of the subcutis.

In various embodiments, the population of cells is cells of the epidermis, and comprises one more of cells of the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and/or stratum germinativum.

In various embodiments, the population of cells is cells of the dermis, and comprises one or more of cells from the papillary region and the reticular region.

In various embodiments, the administration is by subcutaneous injection, intradermal injection, subdermal injection, intramuscular injection, or topical administration.

In various embodiments, the administration is intradermal injection to one or more of the dermis or epidermis.

In various embodiments, the an effective amount is from about 10 ng to about 5000 ng per treatment area of about 10 cm²or less, or about 5 cm²or less, or about 1 cm²or less, or about 0.5 cm²or less, or about 0.2 cm²or less.

In various embodiments, about 10 ng, or about 20 ng, or about 50 ng, or about 100 ng, or about 200 ng, or about 300 ng, or about 400 ng, or about 500 ng, or about 600 ng, or about 700 ng, or about 800 ng, or about 900 ng, or about 1000 ng, or about 1100 ng, or about 1200 ng, or about 1300 ng, or about 1400 ng, or about 1500 ng, or about 1600 ng, or about 1700 ng, or about 1800 ng, or about 1900 ng, or about 2000 ng, or about 3000 ng, or about 4000 ng, or about 5000 ng of the synthetic RNA is administered per treatment area of about 10 cm²or less, or about 5 cm²or less, or about 1 cm²or less, or about 0.5 cm²or less, or about 0.2 cm²or less.

In various embodiments, the modulating results in an increase in the quantity of IL22 in the subject.

In various embodiments, the modulating results in a decrease in the quantity of IL22 in the subject.

In various embodiments, the effective amount of synthetic RNA is administered using an array of needles covering an affected area of the subject.

In various embodiments, the treatment area is mechanically massaged after administration.

In various embodiments, the treatment area is exposed to electric pulses after administration.

In various embodiments, the electric pulses are between about 10V and about 200V for from about 50 microseconds to about 1 second.

In various embodiments, the electric pulses are generated around the treatment area by a multielectrode array.

In some aspects, the present invention relates to a method for treating a metabolic disorder, comprising a step of administering a synthetic RNA encoding a gene-editing protein targeting a defective gene, wherein the administration is by intraportal injection.

In some aspects, the present invention relates to a method for treating a metabolic disorder comprising a step of (a) obtaining a cell comprising a defective gene and (b) contacting the cell with a synthetic RNA encoding a gene-editing protein capable of creating a double strand break in the defective gene, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity. In various embodiments, the method further comprises steps of (c) obtaining the cell contacted in step (b); and (d) administering the cell to a subject in need thereof by intraportal injection.

In various embodiments, the gene-editing protein is selected from a CRISPR/Cas9, TALEN and a zinc finger nuclease.

In various embodiments, the nuclease domain is capable of forming a dimer with another nuclease domain.

In various embodiments, the nuclease domain comprises the catalytic domain of a protein comprising the amino acid sequence of SEQ ID NO: 632.

In various embodiments, the metabolic disorder is selected from a disorder of carbohydrate metabolism, a disorder of amino acid metabolism, a disorder of the urea cycle, a disorder of fatty acid metabolism, a disorder of porphyrin metabolism, a disorder of lysosomal storage, a disorder of peroxisome biogenesis, and a disorder of purine or pyrimidine metabolism.

In various embodiments, the metabolic disorder is a disorder of carbohydrate metabolism and wherein the disease is galactosemia and the defective gene is optionally GALT, GALK1, or GALE; wherein the disease is essential fructosuria and the defective gene is optionally KHK; wherein the disease is Hereditary fructose intolerance and the defective gene is optionally ALDOB; wherein the disease is glycogen storage disease type I and the defective gene is optionally G6PC, SLC37A4, or SLC17A3; wherein the disease is glycogen storage disease type II and the defective gene is optionally GAA; wherein the disease is glycogen storage disease type III and the defective gene is optionally AGL; wherein the disease is glycogen storage disease type IV and the defective gene is optionally GBE1; wherein the disease is glycogen storage disease type V and the defective gene is optionally PYGM; wherein the disease is glycogen storage disease type VI and the defective gene is optionally PYGL; wherein the disease is glycogen storage disease type VII and the defective gene is optionally PYGM; wherein the disease is glycogen storage disease type IX and the defective gene is optionally PHKA1, PHKA2, PHKB, PHKG1, or PHKG2; wherein the disease is glycogen storage disease type XI and the defective gene is optionally SLC2A2; wherein the disease is glycogen storage disease type XII and the defective gene is optionally ALDOA; wherein the disease is glycogen storage disease type XIII and the defective gene is optionally ENO1, ENO2, or ENO3; wherein the disease is glycogen storage disease type 0 and the defective gene is optionally GYS1 or GYS2; wherein the disease is pyruvate carboxylase deficiency and the defective gene is optionally PC; wherein the disease is pyruvate kinase deficiency and the defective gene is optionally PKLR; wherein the disease is transaldolase deficiency and the defective gene is optionally TALDO1; wherein the disease is triosephosphate isomerase deficiency and the defective gene is optionally TPI1; wherein the disease is fructose bisphosphatase deficiency and the defective gene is optionally FBP1; wherein the disease is hyperoxaluria and the defective gene is optionally AGXT or GRHPR; wherein the disease is hexokinase deficiency and the defective gene is optionally HK1; wherein the disease is glucose-galactose malabsorption and the defective gene is optionally SLC5A1; or wherein the disease is glucose-6-phosphate dehydrogenase deficiency and the defective gene is optionally G6PD.

In various embodiments, the metabolic disorder is a disorder of amino acid metabolism wherein the disease is alkaptonuria and the defective gene is optionally HGD; wherein the disease is aspartylglucosaminuria and the defective gene is optionally AGA; wherein the disease is methylmalonic acidemia and the defective gene is optionally MUT, MCEE, MMAA, MMAB, MMACHC, MMADHC, or LMBRD1; wherein the disease is maple syrup urine disease and the defective gene is optionally BCKDHA, BCKDHB, DBT, or DLD; wherein the disease is homocystinuria and the defective gene is optionally CBS; wherein the disease is tyrosinemia and the defective gene is optionally FAH, TAT, or HPD; wherein the disease is trimethylaminuria and the defective gene is optionally FMO3; wherein the disease is Hartnup disease and the defective gene is optionally SLC6A19; wherein the disease is biotinidase deficiency and the defective gene is optionally BTD; wherein the disease is ornithine carbamoyltransferase deficiency and the defective gene is optionally OTC; wherein the disease is carbamoyl-phosphate synthase I deficiency disease and the defective gene is optionally CPS1; wherein the disease is citrullinemia and the defective gene is optionally ASS or SLC25A13; wherein the disease is hyperargininemia and the defective gene is optionally ARG1; wherein the disease is hyperhomocysteinemia and the defective gene is optionally MTHFR; wherein the disease is hypermethioninemia and the defective gene is optionally MAT1A, GNMT, or AHOY; wherein the disease is hyperlysinemias and the defective gene is optionally AASS; wherein the disease is nonketotic hyperglycinemia and the defective gene is optionally GLDC, AMT, or GCSH; wherein the disease is Propionic acidemia and the defective gene is optionally PCCA or PCCB; wherein the disease is hyperprolinemia and the defective gene is optionally ALDH4A1 or PRODH; wherein the disease is cystinuria and the defective gene is optionally SLC3A1 or SLC7A9; wherein the disease is dicarboxylic aminoaciduria and the defective gene is optionally SLC1A1; wherein the disease is glutaric acidemia type 2 and the defective gene is optionally ETFA, ETFB, or ETFDH; wherein the disease is isovaleric acidemia and the defective gene is optionally IVD; or wherein the disease is 2-hydroxyglutaric aciduria and the defective gene is optionally L2HGDH or D2HGDH.

In various embodiments, the metabolic disorder is a disorder of the urea cycle wherein the disease is N-acetylglutamate synthase deficiency and the defective gene is optionally NAGS; wherein the disease is argininosuccinic aciduria and the defective gene is optionally ASL; or wherein the disease is argininemia and the defective gene is optionally ARG1.

In various embodiments, the metabolic disorder is a disorder of fatty acid metabolism wherein the disease is very long-chain acyl-coenzyme A dehydrogenase deficiency and the defective gene is optionally ACADVL; wherein the disease is long-chain 3-hydroxyacyl-coenzyme A dehydrogenase deficiency and the defective gene is optionally HADHA; wherein the disease is medium-chain acyl-coenzyme A dehydrogenase deficiency and the defective gene is optionally ACADM; wherein the disease is short-chain acyl-coenzyme A dehydrogenase deficiency and the defective gene is optionally ACADS; wherein the disease is 3-hydroxyacyl-coenzyme A dehydrogenase deficiency and the defective gene is optionally HADH; wherein the disease is 2,4 dienoyl-CoA reductase deficiency and the defective gene is optionally NADK2; wherein the disease is 3-hydroxy-3-methylglutaryl-CoA lyase deficiency and the defective gene is optionally HMGCL; wherein the disease is malonyl-CoA decarboxylase deficiency and the defective gene is optionally MLYCD; wherein the disease is systemic primary carnitine deficiency and the defective gene is optionally SLC22A5; wherein the disease is carnitine-acylcarnitine translocase deficiency and the defective gene is optionally SLC25A20; wherein the disease is carnitine palmitoyltransferase I deficiency and the defective gene is optionally CPT1A; wherein the disease is carnitine palmitoyltransferase II deficiency and the defective gene is optionally CPT2; wherein the disease is lysosomal acid lipase deficiency and the defective gene is optionally LIPA; or wherein the disease is Gaucher's disease and the defective gene is optionally GBA.

In various embodiments, the metabolic disorder is a disorder of porphyrin metabolism wherein the disease is acute intermittent porphyria and the defective gene is optionally HMBS; wherein the disease is Gunther disease and the defective gene is optionally UROS; wherein the disease is porphyria cutanea tarda and the defective gene is optionally UROD; wherein the disease is hepatoerythropoietic porphyria and the defective gene is optionally UROD; wherein the disease is hereditary coproporphyria and the defective gene is optionally CPOX; wherein the disease is variegate porphyria and the defective gene is optionally PPOX; wherein the disease is erythropoietic protoporphyria and the defective gene is optionally FECH; or wherein the disease is aminolevulinic acid dehydratase deficiency porphyria and the defective gene is optionally ALAD.

In various embodiments, the metabolic disorder is a disorder of lysosomal storage wherein the disease is Farber disease and the defective gene is optionally ASAH1; wherein the disease is Krabbe disease and the defective gene is optionally GALC; wherein the disease is galactosialidosis and the defective gene is optionally CTSA; wherein the disease is fabry disease and the defective gene is optionally GLA; wherein the disease is Schindler disease and the defective gene is optionally NAGA; wherein the disease is GM1 gangliosidosis and the defective gene is optionally GLB1; wherein the disease is Tay-Sachs disease and the defective gene is optionally HEXA; wherein the disease is Sandhoff disease and the defective gene is optionally HEXB; wherein the disease is GM2-gangliosidosis, AB variant and the defective gene is optionally GM2A; wherein the disease is Niemann-Pick disease and the defective gene is optionally SMPD1, NPC1, or NPC2; wherein the disease is metachromatic leukodystrophy and the defective gene is optionally ARSA or PSAP; wherein the disease is multiple sulfatase deficiency and the defective gene is optionally SUMF1; wherein the disease is Hurler syndrome and the defective gene is optionally IDUA; wherein the disease is Hunter syndrome and the defective gene is optionally IDS; wherein the disease is Sanfilippo syndrome and the defective gene is optionally SGSH, NAGLU, HGSNAT, or GNS; wherein the disease is Morquio syndrome and the defective gene is optionally GALNS or GLB1; wherein the disease is Maroteaux-Lamy syndrome and the defective gene is optionally ARSB; wherein the disease is Sly syndrome and the defective gene is optionally GUSB; wherein the disease is sialidosis and the defective gene is optionally NEU1, NEU2, NEU3, or NEU4; wherein the disease is I-cell disease and the defective gene is optionally GNPTAB or GNPTG; wherein the disease is mucolipidosis type IV and the defective gene is optionally MCOLN1; wherein the disease is infantile neuronal ceroid lipofuscinosis and the defective gene is optionally PPT1 or PPT2; wherein the disease is Jansky-Bielschowsky disease and the defective gene is optionally TPP1; wherein the disease is Batten disease and the defective gene is optionally CLN1, CLN2, CLN3, CLN5, CLN6, MFSD8, CLN8, or CTSD; wherein the disease is Kufs disease, Type A and the defective gene is optionally CLN6 or PPT1; wherein the disease is Kufs disease, Type B and the defective gene is optionally DNAJC5 or CTSF; wherein the disease is alpha-mannosidosis and the defective gene is optionally MAN2B1, MAN2B2, or MAN2C1; wherein the disease is beta-mannosidosis and the defective gene is optionally MANBA; wherein the disease is fucosidosis and the defective gene is optionally FUCA1; wherein the disease is cystinosis and the defective gene is optionally CTNS; wherein the disease is pycnodysostosis and the defective gene is optionally CTSK; wherein the disease is Salla disease and the defective gene is optionally SLC17A5; wherein the disease is Infantile free sialic acid storage disease and the defective gene is optionally SLC17A5; or wherein the disease is Danon disease and the defective gene is optionally LAMP2.

In various embodiments, the metabolic disorder is a disorder of peroxisome biogenesis wherein the disease is Zellweger syndrome and the defective gene is optionally PEX1, PEX2, PEX3, PEX5, PEX6, PEX12, PEX14, or PEX26; wherein the disease is Infantile Refsum disease and the defective gene is optionally PEX1, PEX2, or PEX26; wherein the disease is neonatal adrenoleukodystrophy and the defective gene is optionally PEX5, PEX1, PEX10, PEX13, or PEX26; wherein the disease is RCDP Type 1 and the defective gene is optionally PEX7; wherein the disease is pipecolic acidemia and the defective gene is optionally PAHX; wherein the disease is acatalasia and the defective gene is optionally CAT; wherein the disease is hyperoxaluria type 1 and the defective gene is optionally AGXT; wherein the disease is Acyl-CoA oxidase deficiency and the defective gene is optionally ACOX1; wherein the disease is D-bifunctional protein deficiency and the defective gene is optionally HSD17B4; wherein the disease is dihydroxyacetonephosphate acyltransferase deficiency and the defective gene is optionally GNPAT; wherein the disease is X-linked adrenoleukodystrophy and the defective gene is optionally ABCD1; wherein the disease is α-methylacyl-CoA racemase deficiency and the defective gene is optionally AMACR; wherein the disease is RCDP Type 2 and the defective gene is optionally DHAPAT; wherein the disease is RCDP Type 3 and the defective gene is optionally AGPS; wherein the disease is adult refsum disease-1 and the defective gene is optionally PHYH; or wherein the disease is mulibrey nanism and the defective gene is optionally TRIM37.

In various embodiments, the metabolic disorder is a disorder of purine or pyrimidine metabolism wherein the disease is Lesch-Nyhan syndrome and the defective gene is optionally HPRT; wherein the disease is adenine phosphoribosyltransferase deficiency and the defective gene is optionally APRT; wherein the disease is adenosine deaminase deficiency and the defective gene is optionally ADA; wherein the disease is Adenosine monophosphate deaminase deficiency type 1 and the defective gene is optionally AMPD1; wherein the disease is adenylosuccinate lyase deficiency and the defective gene is optionally ADSL; wherein the disease is dihydropyrimidine dehydrogenase deficiency and the defective gene is optionally DPYD; wherein the disease is Miller syndrome and the defective gene is optionally DHODH; wherein the disease is orotic aciduria and the defective gene is optionally UMPS; wherein the disease is purine nucleoside phosphorylase deficiency and the defective gene is optionally PNP; or wherein the disease is xanthinuria and the defective gene is optionally XDH, MOCS1, or MOCS2, GEPH.

In some aspects, the present invention relates to a method for modulating transthyretin (TTR). The method comprising a step of administering an effective amount of a synthetic RNA encoding TTR to a subject, wherein the synthetic RNA comprises one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In various embodiments, the modulating results in an increase in the quantity of TTR in the subject.

In various embodiments, the modulating results in a decrease in the quantity of TTR in the subject.

In various embodiments, the modulating results in treatment of one or more of an amyloid disease, senile systemic amyloidosis (SSA), familial amyloid polyneuropathy (FAP), and familial amyloid cardiomyopathy (FAC).

In various embodiments, the non-canonical nucleotides have one or more substitutions at positions selected from the 2C, 4C, and 5C positions for a pyrimidine, or selected from the 6C, 7N and 8C positions for a purine.

In various embodiments, the non-canonical nucleotides comprise one or more of 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, pseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine, optionally at an amount of at least 50%, or at least 60%, or at least 70%, or at least 80%, or at least 90%, or 100% of the non-canonical nucleotides.

In various embodiments, the at least about 50% of cytidine residues are non-canonical nucleotides, and which are selected from 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, and 5-methoxycytidine.

In various embodiments, the at least about 75% or at least about 90% of cytidine residues are non-canonical nucleotides, and the non-canonical nucleotides are selected from 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, and 5-methoxycytidine.

In various embodiments, the at least about 20% of uridine, or at least about 40%, or at least about 50%, or at least about 75%, or at about least 90% of uridine residues are non-canonical nucleotides, and the non-canonical are selected from pseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine.

In various embodiments, the at least about 40%, or at least about 50%, or at least about 75%, or at about least 90% of uridine residues are non-canonical nucleotides, and the non-canonical nucleotides are selected from pseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine.

In various embodiments, the at least about 10% of guanine residues are non-canonical nucleotides, and the non-canonical nucleotide is optionally 7-deazaguanosine.

In various embodiments, the synthetic RNA contains no more than about 50% 7-deazaguanosine in place of guanosine residues.

In various embodiments, the synthetic RNA does not contain non-canonical nucleotides in place of adenosine residues.

In various embodiments, the synthetic RNA comprises a 5′ cap structure.

In various embodiments, the synthetic RNA 5′-UTR comprises a Kozak consensus sequence.

In various embodiments, the synthetic RNA 5′-UTR comprises a sequence that increases RNA stability in vivo, and the 5′-UTR may comprise an alpha-globin or beta-globin 5′-UTR.

In various embodiments, the synthetic RNA 3′-UTR comprises a sequence that increases RNA stability in vivo, and the 3′-UTR may comprise an alpha-globin or beta-globin 3′-UTR.

In various embodiments, the synthetic RNA comprises a 3′ poly(A) tail.

In various embodiments, the synthetic RNA 3′ poly(A) tail is from about 20 nucleotides to about 250 nucleotides in length.

In various embodiments, the synthetic RNA is from about 200 nucleotides to about 5000 nucleotides in length.

In various embodiments, the synthetic RNA is from about 500 to about 2000 nucleotides in length, or about 500 to about 1500 nucleotides in length, or about 500 to about 1000 nucleotides in length.

In various embodiments, the synthetic RNA is prepared by in vitro transcription.

In various embodiments, the effective amount of the synthetic RNA is administered as one or more injections containing about 10 ng to about 5000 ng of RNA.

In various embodiments, the effective amount of the synthetic RNA is administered as one or more injections each containing no more than about 10 ng, or no more than about 20 ng, or no more than about 50 ng, or no more than about 100 ng, or no more than about 200 ng, or no more than about 300 ng, or no more than about 400 ng, or no more than about 500 ng, or no more than about 600 ng, or no more than about 700 ng, or no more than about 800 ng, or no more than about 900 ng, or no more than about 1000 ng, or no more than about 1100 ng, or no more than about 1200 ng, or no more than about 1300 ng, or no more than about 1400 ng, or no more than about 1500 ng, or no more than about 1600 ng, or no more than about 1700 ng, or no more than about 1800 ng, or no more than about 1900 ng, or no more than about 2000 ng, or no more than about 3000 ng, or no more than about 4000 ng, or no more than about 5000 ng.

In various embodiments, the effective amount of the synthetic RNA is administered as one or more injections each containing about 10 ng, or about 20 ng, or about 50 ng, or about 100 ng, or about 200 ng, or about 300 ng, or about 400 ng, or about 500 ng, or about 600 ng, or about 700 ng, or about 800 ng, or about 900 ng, or about 1000 ng, or about 1100 ng, or about 1200 ng, or about 1300 ng, or about 1400 ng, or about 1500 ng, or about 1600 ng, or about 1700 ng, or about 1800 ng, or about 1900 ng, or about 2000 ng, or about 3000 ng, or about 4000 ng, or about 5000 ng.

In various embodiments, the effective amount of the synthetic RNA comprises one or more lipids to enhance uptake of RNA by cells.

In various embodiments, the effective amount of the synthetic RNA comprises a cationic liposome formulation and the lipids are optionally selected from Table 1.

In various embodiments, the subject is a human.

In various embodiments, the effective amount of the synthetic RNA is administered about weekly, for at least 2 weeks.

In various embodiments, the effective amount of the synthetic RNA is administered about every other week for at least one month.

In various embodiments, the effective amount of the synthetic RNA is administered monthly or about every other month.

In various embodiments, the effective amount of the synthetic RNA is administered for at least two months, or at least 4 months, or at least 6 months, or at least 9 months, or at least one year.

In some aspects, the present invention relates to a composition comprising an effective amount of the synthetic RNA used in any herein-disclosed aspect or embodiment.

In some aspects, the present invention relates to a pharmaceutical composition, comprising the composition of any herein-disclosed aspect or embodiment and a pharmaceutically acceptable excipient.

In some aspects, the present invention relates to the use of a composition or pharmaceutical composition of any herein-disclosed aspect or embodiment in the treatment of a disease or disorder described herein.

In some aspects, the present invention relates to the use of a composition or pharmaceutical composition of any herein-disclosed aspect or embodiment in the manufacture of a medicament for the treatment of a disease or disorder described herein.

In various aspects, the present invention is based on the surprising discovery of safe and effective doses and administration parameters for nucleic acid drugs in human subjects. In some aspects, there is provided a method for delivering a nucleic acid drug, comprising administering an effective dose of a nucleic acid drug to a human subject in need thereof. In various embodiments, the nucleic acid drug comprises a RNA comprising one or more non-canonical nucleotides (a/k/a “modified RNA”). In other embodiments, the effective dose is an amount sufficient to substantially increase an amount of a protein encoded by the nucleic acid drug in the human subject and/or substantially avoid an immune reaction in a human subject, wherein the immune reaction is optionally mediated by the innate immune system.

In some aspects, there is provided a method for expressing a protein of interest in a population of cells in a mammalian subject, comprising administering a non-viral transfection composition comprising an effective dose of a RNA encoding the protein of interest to said cells, where the transfection composition is administered in an amount that allows for expression of said protein in said cells for at least about six hours to about five days without substantial cellular toxicity. In some embodiments, the RNA contains one or more non-canonical nucleotides that avoid substantial cellular toxicity.

In some embodiments, the effective dose is about 100 ng to about 5000 ng (e.g., about, or no more than about, 100 ng, or 200 ng, or 300 ng, or 400 ng, or 500 ng, or 600 ng, or 700 ng, or 800 ng, or 900 ng, or 1000 ng, or 1100 ng, or 1200 ng, or 1300 ng, or 1400 ng, or 1500 ng, or 1600 ng, or 1700 ng, or 1800 ng, or 1900 ng, or 2000 ng, or 3000 ng, or 4000 ng, or 5000 ng). In other embodiments, the effective dose is less than about 100 ng. In certain embodiments, the effective dose is about 10 ng to about 100 ng (e.g., about, or no more than about, 10 ng, or 20 ng, or 30 ng, or 40 ng, or 50 ng, or 60 ng, or 70 ng, or 80 ng, or 90 ng, or 100 ng).

In some embodiments, the effective dose is about 1.4 ng/kg to about 30 ng/kg (e.g., about, or no more than about, 1.4 ng/kg, or 2.5 ng/kg, or 5 ng/kg, or 10 ng/kg, or 15 ng/kg, or 20 ng/kg, or 25 ng/kg, or 30 ng/kg. In other embodiments, the effective dose is less than about 1.5 ng/kg. In certain embodiments, the effective dose is about 0.14 ng/kg to about 1.4 ng/kg (e.g., about, or no more than about, 0.14 ng/kg, or 0.25 ng/kg, or 0.5 ng/kg, or 0.75 ng/kg, or 1 ng/kg, or 1.25 ng/kg, or 1.4 ng/kg).

In some embodiments, the effective dose is about 350 ng/cm²to about 7000 ng/cm²(e.g., about, or no more than about, 350 ng/cm², or 500 ng/cm², or 750 ng/cm², or 1000 ng/cm², or 2000 ng/cm², or 3000 ng/cm², or 4000 ng/cm², or 5000 ng/cm², or 6000 ng/cm², or 7000 ng/cm²). In other embodiments, the effective dose is less than about 350 ng/cm². In certain embodiments, the effective dose is about 35 ng/cm²to about 350 ng/cm²(e.g., about, or no more than about, 35 ng/cm², or 50 ng/cm², or 75 ng/cm², or 100 ng/cm², or 150 ng/cm², or 200 ng/cm², or 250 ng/cm², or 300 ng/cm², or 350 ng/cm².

In some embodiments, the effective dose is about 0.28 picomoles to about 5.7 picomoles (e.g., about, or no more than about, 0.28 picomoles, or 0.5 picomoles, or 0.75 picomoles, or 1 picomole, or 2 picomoles, or 3 picomoles, or 4 picomoles, or 5 picomoles, or 5.7 picomoles). In other embodiments, the effective dose is less than about 0.28 picomoles. In certain embodiments, the effective dose is about 0.028 picomoles to about 0.28 picomoles (e.g., about, or no more than about, 0.028 picomoles, or 0.05 picomoles, or 0.075 picomoles, or 0.1 picomoles, or 0.15 picomoles, or 0.2 picomoles, or 0.25 picomoles, or 0.28 picomoles).

In some embodiments, the effective dose is about 0.004 picomoles/kg to about 0.082 picomoles/kg (e.g., about, or no more than about, 0.004 picomoles/kg, or 0.01 picomoles/kg, or 0.02 picomoles/kg, or 0.03 picomoles/kg, or 0.04 picomoles/kg, or 0.05 picomoles/kg, or 0.06 picomoles/kg, or 0.07 picomoles/kg, or 0.08 picomoles/kg, or 0.082 picomoles/kg). In other embodiments, the effective dose is less than about 0.004 picomoles/kg. In certain embodiments, the effective dose is about 0.0004 picomoles/kg to about 0.004 picomoles/kg (e.g., about, or no more than about, 0.0004 picomoles/kg, or 0.001 picomoles/kg, or 0.002 picomoles/kg, or 0.003 picomoles/kg, or 0.004 picomoles/kg).

In some embodiments, the effective dose is about 1 picomole/cm²to about 20 picomoles/cm²(e.g., about, or no more than about, 1 picomole/cm², or 2 picomoles/cm², or 3 picomoles/cm², or 4 picomoles/cm², or 5 picomoles/cm², or 6 picomoles/cm², or 7 picomoles/cm², or 8 picomoles/cm², or 9 picomoles/cm², or 10 picomoles/cm², or 12 picomoles/cm², or 14 picomoles/cm², or 16 picomoles/cm², or 18 picomoles/cm², or 20 picomoles/cm²). In other embodiments, the effective dose is less than about 1 picomole/cm². In certain embodiments, the effective dose is about 0.1 picomoles/cm²to about 1 picomole/cm²(e.g., about, or no more than about, 0.1 picomoles/cm², or 0.2 picomoles/cm², or 0.3 picomoles/cm², or 0.4 picomoles/cm², or 0.5 picomoles/cm², or 0.6 picomoles/cm², or 0.7 picomoles/cm², or 0.8 picomoles/cm², or 0.9 picomoles/cm², or 1 picomole/cm²).

In various embodiments, the nucleic acid drug is administered in a pharmaceutically acceptable formulation, including a formulation suitable for one or more of injection (e.g. subcutaneous injection, intradermal injection (including to the dermis or epidermis), subdermal injection, intramuscular injection, intraocular injection, intravitreal injection, intra-articular injection, intracardiac injection, intravenous injection, epidural injection, intrathecal injection, intraportal injection, intratumoral injection) and topical administration and/or for administration to the integumentary system (e.g. to one or more of the epidermis (optionally selected from the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum germinativum), basement membrane, dermis (optionally selected from the papillary region and the reticular region), subcutis, and conjunctiva) and/or for administration to the eye (e.g., to one or more of the cornea, sclera, iris, lens, corneal limbus, optic nerve, choroid, ciliary body, anterior segment, anterior chamber, and retina).

In various embodiments, the nucleic acid drug is formulated with one or more lipids to enhance uptake of the nucleic acid drug by cells, the lipids optionally selected from Table 1. In other embodiments, the nucleic acid drug is formulated with one or more nanoparticles, optionally lipid or polymeric nanoparticles, to enhance uptake of the nucleic acid drug by cells, to enhance duration of protein expression, or to otherwise enhance the safety and/or efficacy of the nucleic acid drug.

In various embodiments, the nucleic acid drug is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose is administered to a surface area of about 4 mm²to about 1000 mm²(e.g. about, or no more than about, 4 mm², or 5 mm², or 10 mm², or 25 mm², or 50 mm², or 75 mm², or 100 mm², or 125 mm², or 150 mm², or 200 mm², or 500 mm², or 1000 mm²).

In various embodiments, the nucleic acid drug is administered in a treatment regimen, optionally with an additional agent or adjuvant therapy described herein, and the administration is about weekly to about once every 24 weeks (e.g. about, or not more than about, weekly, or once every 2 weeks, or once every 3 weeks, or once every 4 weeks, or once every 5 weeks, or once every 6 weeks, or once every 7 weeks, or once every 8 weeks, or once every 9 weeks, or once every 9 weeks, or once every 9 weeks, or once every 9 weeks, or once every 10 weeks, or once every 11 weeks, or once every 12 weeks, or once every 13 weeks, or once every 14 weeks, or once every 15 weeks, or once every 20 weeks, or once every 24 weeks). In other embodiments, the nucleic acid drug is administered in a treatment regimen, optionally with an additional agent or adjuvant therapy described herein, and the administration is about daily to about weekly (e.g., about, or not more than about, daily, or once every 2 days, or once every 3 days, or once every 4 days, or once every 5 days, or once every 6 days, or weekly).

In various embodiments the nucleic acid drug comprises RNA comprising one or more non-canonical nucleotides, optionally having one or more substitutions at the 2C and/or 4C and/or 5C positions for a pyrimidine or the 6C and/or 7N and/or 8C positions for a purine. In various embodiments, the non-canonical nucleotide is one or more of the non-canonical nucleotides described herein, including, for example, 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, pseudouridine, 5-hydroxyuridine, 5-hydroxypseudouridine, 5-methyluridine, 5-methylpseudouridine, 5-hydroxymethyluridine, 5-hydroxymethylpseudouridine, 5-carboxyuridine, 5-carboxypseudouridine, 5-formyluridine, 5-formylpseudouridine, 5-methoxyuridine, and 5-methoxypseudouridine. Further, the RNA comprising one or more non-canonical nucleotides may have one or more of a 5′-UTR comprising a Kozak consensus sequence, a 5′-UTR or 3′-UTR comprising a sequence that increases RNA stability in vivo (e.g. an alpha-globin or beta-globin 5′-UTR or an alpha-globin or beta-globin 3′-UTR), and a 3′ poly(A) tail from about 20 nucleotides to about 250 nucleotides in length (e.g. about 20, or about 30, or about 40, or about 50, or about 60, or about 70, or about 80, or about 90, or about 100, or about 110, or about 120, or about 130, or about 140, or about 150, or about 160, or about 170, or about 180, or about 190, or about 200, or about 210, or about 220, or about 230, or about 240, or about 250 nucleotides in length).

Further, some aspects of the methods described herein find use in various medical treatments, including, by way of illustration, treating a disease, disorder and/or condition of the integumentary system or altering, modifying and/or changing the integumentary system (e.g. cosmetically).

Also contemplated are kits suitable for use in human therapy, comprising the nucleic acid drug described herein in a unit dosage form of about 10 ng to about 5000 ng (e.g. about, or no more than about, 10 ng, or 20 ng, or 50 ng, or 100 ng, or 200 ng, or 300 ng, or 400 ng, or 500 ng, or 600 ng, or 700 ng, or 800 ng, or 900 ng, or 1000 ng, or 1100 ng, or 1200 ng, or 1300 ng, or 1400 ng, or 1500 ng, or 1600 ng, or 1700 ng, or 1800 ng, or 1900 ng, or 2000 ng, or 3000 ng, or 4000 ng, or 5000 ng) and an injection needle.

Further, in some aspects, the present invention provides a pharmaceutical formulation comprising the nucleic acid drug described herein and one or more of the vehicles (a/k/a “transfection reagents”, e.g., lipids) described herein, the formulation optionally being suitable for one or more of subcutaneous injection, intradermal injection, subdermal injection, intramuscular injection, intraocular injection, intravitreal injection, intra-articular injection, intracardiac injection, intravenous injection, epidural injection, intrathecal injection, intraportal injection, intratumoral injection, and topical administration. As used herein, the term “injection” can refer to injection, for example, with a syringe, and to other methods of administering liquids, for example, infusion, perfusion, administration using a pen injector, cartridge system needle-array or patch and/or administration by a catheter system.

In some aspects, nucleic acid delivery patches are provided. In one aspect, devices for delivering nucleic acids using electric fields are provided. Other aspects pertain to methods and compositions for delivery of nucleic acids to the skin. Still further aspects pertain to methods and compositions for expression of proteins in the skin.

In one aspect, the invention provides methods and compositions for treating diseases and conditions in humans, including, but not limited to, prophylactic treatments, treatments for rare diseases, including, but not limited to, dermatologic rare diseases, and treatments for use in medical dermatology and aesthetic medicine. In another aspect, the invention provides cosmetics comprising nucleic acids. Still further aspects relate to methods and compositions for altering pigmentation, for example, for the treatment of pigmentation disorders. Still further aspects relate to methods and compositions for enhancing healing, including, but not limited to, healing in response to a wound or surgery. The compositions of the present invention may alter, modify and/or change the appearance of a member of the integumentary system of a subject such as, but not limited, to skin, hair and nails. Such alteration, modification and/or change may be in the context of treatment methods and/or therapeutic uses as described herein including, by way of non-limiting example, dermatological treatments and cosmetics procedures.

Further, in various embodiments, the present invention relates to the targeting of various therapeutic proteins that are not limited to dermatological applications. For example, in various embodiments, the present compositions and methods find use in methods of treatment that are mediated by the increased expression of, for example, various soluble proteins as illustrated herein. In various embodiments, the nucleic acid drug encodes and/or increases the expression of one or more of a circulating protein, an extracellular matrix protein, an engineered protein, a gene-editing protein, a protein or peptide hormone, an enzyme, erythropoietin, darbepoetin alfa, NOVEPOETIN, elastin, collagen, an antibody or antibody fragment (e.g., a neutralizing antibody or antibody fragment), an intracellular protein, telomerase reverse transcriptase, a membrane protein, a fusion protein, a receptor, a ligand binding domain, a protein inhibitor, or a biologically active fragment, analogue or variant thereof. In other embodiments, administration of the nucleic acid drug results in one or more of an increase in hematocrit, an increase in tissue elasticity, an increase in tissue strength, and increase in skin hydration and/or water retention, hair growth, fat reduction, an insertion, deletion or mutation in DNA, conversion of a prodrug to an active drug, a decrease in tumor size and/or number, a decrease in plaque size and/or number, an increase in vascularization, a decrease in vascularization, an increase in visual acuity, a decrease in pain, an increase in cardiac output (e.g., ejection fraction and stroke volume), a decrease in abnormal heart rhythm, a decrease in fibrosis, a decrease in one or more adverse neurological symptoms, conditions, or disorders (e.g., depression, dysregulation of appetite, polyphagia, anorexia, dementia, headache, fatigue, numbness, tremors, and dizziness), a decrease in erectile dysfunction, an increase in vitality, an increase in pulmonary function, an increase in kidney function, an increase in liver function, an increase in insulin sensitivity, a decrease in insulin sensitivity, a decrease in inflammation, an increase in tear production, an improvement in hearing, an increase in auditory perception, a decrease in tinnitus, a reduction in perspiration, partial or total clearance of an infection, an increase in fertility, a decrease in fertility, inhibition or neutralization of a protein, recruitment or stimulation of one or more components of the immune system, lengthening of telomeres, inhibition of cellular senescence, an increase in replicative potential, reprogramming, proliferation, differentiation, and an increase in differentiation potential.

In some aspects, RNA molecules with low toxicity and high translation efficiency are provided. In one aspect, a cell-culture medium for high-efficiency in vivo transfection, reprogramming, and gene editing of cells is provided. Other aspects pertain to methods for producing RNA molecules encoding reprogramming proteins. Still further aspects pertain to methods for producing RNA molecules encoding gene-editing proteins.

In one aspect, the invention provides high-efficiency gene-editing proteins comprising engineered nuclease cleavage domains. In another aspect, the invention provides high-fidelity gene-editing proteins comprising engineered nuclease cleavage domains. Other aspects relate to high-efficiency gene-editing proteins comprising engineered DNA-binding domains. Still further aspects pertain to high-fidelity gene-editing proteins comprising engineered DNA-binding domains. Still further aspects relate to gene-editing proteins comprising engineered repeat sequences. Still further aspects relate to gene-editing proteins comprising DNA-modification domains. Some aspects relate to methods for altering the DNA sequence of a cell by transfecting the cell with or inducing the cell to express a gene-editing protein. Other aspects relate to methods for altering the DNA sequence of a cell that is present in an in vitro culture. Still further aspects relate to methods for altering the DNA sequence of a cell that is present in vivo.

In some aspects, the invention provides methods for treating cancer comprising administering to a patient a therapeutically effective amount of a gene-editing protein or a nucleic-acid encoding a gene-editing protein. In one aspect, the gene-editing protein is capable of altering the DNA sequence of a cancer associated gene. In another aspect, the cancer-associated gene is the BIRC5 gene. In other aspects, the gene-editing protein is capable of altering the DNA sequence of a cell to cause the cell to express an antigen or antigen receptor. In one aspect, the antigen is a tumor antigen. In another aspect, the antigen receptor binds to a tumor antigen. In yet another aspect, the antigen receptor is a chimeric antigen receptor. Still other aspects relate to therapeutics comprising nucleic acids and/or cells and methods of using therapeutics comprising nucleic acids and/or cells for the treatment of, for example, type 1 diabetes, heart disease, including ischemic and dilated cardiomyopathy, macular degeneration, Parkinson's disease, cystic fibrosis, sickle-cell anemia, thalassemia, Fanconi anemia, severe combined immunodeficiency, hereditary sensory neuropathy, xeroderma pigmentosum, Huntington's disease, muscular dystrophy, amyotrophic lateral sclerosis, Alzheimer's disease, cancer, and infectious diseases including hepatitis and HIV/AIDS. In some aspects, the nucleic acids comprise RNA. In other aspects, the RNA comprises one or more non-canonical nucleotides. In still other aspects, the nucleic acids are delivered to cells using a virus. In some aspects, the virus is a replication-competent virus. In other aspects, the virus is a replication-incompetent virus.

The details of the invention are set forth in the accompanying description below. Although methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention, illustrative methods and materials are now described. Other features, objects, and advantages of the invention will be apparent from the description and from the claims. In the specification and the appended claims, the singular forms also include the plural unless the context clearly dictates otherwise. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

Any aspect or embodiment disclosed herein can be combined with any other aspect or embodiment as disclosed herein.

DETAILED DESCRIPTION OF THE FIGURES

FIG. 1 depicts primary adult human dermal fibroblasts transfected with RNA encoding green fluorescent protein (“GFP”) and comprising the indicated nucleotides.

FIG. 2 depicts the result of a gene-expression analysis of the primary adult human dermal fibroblasts of FIG. 1 using a one-step real-time RT-PCR and primers designed to amplify human interferon beta mRNA. Data were normalized to the untransfected sample (“Neg.”). GAPDH was used as a loading control.

FIG. 3 depicts the results of a gene-expression analysis of cells transfected with RNA comprising the indicated nucleotides, conducted as in FIG. 2. Data were normalized to the untransfected sample (“Neg.”). GAPDH was used as a loading control.

FIG. 4 depicts the results of a gene-expression analysis of cells transfected with RNA comprising the indicated nucleotides, conducted as in FIG. 2, and using primers designed to amplify the indicated transcripts. Data were normalized to the untransfected sample (“Neg.”). GAPDH was used as a loading control.

FIG. 5 depicts the results of a gene-expression analysis of primary human epidermal keratinocytes transfected with RNA encoding NOVEPOETIN, and comprising the indicated nucleotides, conducted as in FIG. 2. Data were normalized to the untransfected sample (“Neg.”). GAPDH was used as a loading control.

FIG. 6 depicts intradermal injection of a solution comprising RNA encoding GFP into the ventral forearm of a healthy, 33 year-old, 70 kg, male human subject.

FIG. 7 depicts a region of the ventral forearm of the subject shown in FIG. 6 after treatment with RNA comprising 5-methoxyuridine and encoding GFP (injection sites 1-3) or COL7 (injection site 4). The image was taken immediately following the final injection.

FIG. 8 depicts the region of FIG. 7, 24 hours after injection.

FIG. 9 depicts the results of fluorescent imaging of the region of FIG. 7, using the indicated fluorescent channels. The dose at each injection site is also indicated. Images were taken 24 hours after injection.

FIG. 10 depicts the results of fluorescent imaging of the region of FIG. 7, using the FITC fluorescent channel. The dose at each injection site is indicated. Images were taken 48 hours after injection.

FIG. 11 depicts the results of quantitative fluorescent imaging of the region of FIG. 7, using the FITC fluorescent channel. The horizontal axis indicates time after injection.

FIG. 12 depicts the results of fluorescent imaging of an independent experiment in which a region of the ventral forearm of as the subject shown in FIG. 6 treated with RNA comprising 5-methoxyuridine and encoding GFP. The image was taken 24 hours after injection.

FIG. 13 depicts the results of an ELISA designed to detect darbepoetin alfa in culture media of primary human epidermal keratinocytes transfected with RNA comprising the indicated nucleotides and encoding NOVEPOETIN.

FIG. 14 depicts the results of an ELISA designed to detect darbepoetin alfa in culture media of primary human epidermal keratinocytes transfected with RNA comprising the indicated nucleotides and encoding NOVEPOETIN.

FIG. 15 depicts the results of an ELISA designed to detect darbepoetin alfa in culture media of primary human epidermal keratinocytes transfected with RNA comprising the indicated nucleotides and encoding NOVEPOETIN.

FIG. 16 depicts primary human dermal fibroblasts transfected with RNA comprising 5-methoxyuridine and encoding hTERT. Cells were fixed and stained using an antibody targeting hTERT 24 hours after transfection.

FIG. 17 depicts primary adult human dermal fibroblasts transfected with RNA encoding green fluorescent protein (“GFP”), prepared and stored as indicated.

FIG. 18 depicts single administration of NOVECRIT induced a rapid increase and sustained level of NOVEPOIETIN in serum. The Y axis shows concentration of NOVEPOIETIN protein (mU/mL).

FIG. 19 depicts a single administration of NOVECRIT stimulated erythropoiesis, yielding elevated hematocrit for at least 14 days. The left panel shows % hematocrit on the Y axis, while the right panel shows % reticulocytes.

FIG. 20 depicts a table summarizing TNFα, IL-6, and IFNα cytokine levels in plasma samples collected from a maximum tolerated dose of NOVECRIT in male Sprague Dawley rats study of Example 35.

FIG. 21 depicts a SURVEYOR assay using the DNA of primary adult human dermal fibroblasts transfected with RNA TALENs targeting the sequence TGAGCAGAAGTGGCTCAGTG (SEQ ID NO: 467) and TGGCTGTACAGCTACACCCC (SEQ ID NO: 468), located within the COL7A1 gene. The bands present in the +RNA lane indicate editing of a region of the gene that is frequently involved in dystrophic epidermolysis bullosa.

FIG. 22 depicts a SURVEYOR assay using the DNA of primary adult human dermal fibroblasts transfected with RNA TALENs targeting the sequence TTCCACTCCTGCAGGGCCCC (SEQ ID NO: 469) and TCGCCCTTCAGCCCGCGTTC (SEQ ID NO:470), located within the COL7A1 gene. The bands present in the +RNA lane indicate editing of a region of the gene that is frequently involved in dystrophic epidermolysis bullosa.

FIG. 23 shows the immunogenicity of various synthetic RNA constructs in the context of a gene-editing (i.e. unmodified nucleotides “A,G,U,C”; pseudouridine only “psU”; 5-methylcytidine only “5mC”; both pseudouridine and 5-methylcytidine “psU+5mC”; and a negative control “neg”).

FIG. 24 shows the gene-editing activity in cells transfected with various synthetic RNA constructs (i.e. unmodified nucleotides “A,G,U,C”; psuedouridine only “psU”; 5-methylcytidine only “5mC”; both psuedouridine and 5-methylcytidine “psU+5mC”; and a negative control “neg”).

FIG. 25 depicts gene editing of the COL7A1 gene in primary human epidermal keratinocytes transfected with RNA encoding TALENs and a single-stranded DNA repair template (“RT”) of the indicated length. The presence of bands at the locations shown by asterisks (“*”) indicates successful gene editing.

FIG. 26 depicts gene editing of the COL7A1 gene in primary human epidermal keratinocytes transfected with RNA encoding TALENs and an 80 nt single-stranded DNA repair template (“RT”) at the indicated ratios of RNA to repair template. The presence of bands at the locations shown by asterisks (“*”) indicates successful gene editing.

FIG. 27 depicts gene correction of the COL7A1 gene in primary human epidermal keratinocytes transfected with RNA encoding TALENs and a single-stranded DNA repair template (“RT”) of the indicated length. The presence of bands at the locations shown by asterisks (“*”) indicates successful gene correction.

FIG. 28 depicts gene correction of the COL7A1 gene in primary human epidermal keratinocytes transfected with RNA encoding TALENs and an 80 nt single-stranded DNA repair template (“RT”) at the indicated ratios of RNA to repair template. The presence of bands at the locations shown by asterisks (“*”) indicates successful gene correction.

FIG. 29 depicts gene editing (“T7E1”) and correction (“Digestion”) of the COL7A1 gene in primary human epidermal keratinocytes transfected with RNA encoding TALENs and an 80 nt single-stranded DNA repair template (“RT”). The presence of bands at the locations shown by asterisks (“*”) indicates successful gene editing (“T7E1”) and correction (“Digestion”).

FIG. 30 depicts the amount of BMP7 protein secreted by primary human dermal fibroblasts and primary human epidermal keratinocytes transfected with RNA encoding the indicated BMP7 variant, measured by ELISA. Asterisks (“*”) indicate saturation of the ELISA assay.

FIG. 31 depicts the amount of parathyroid hormone secreted by primary human epidermal keratinocytes transfected with RNA encoding PTH, measured by ELISA.

FIG. 32 demonstrates that repeated administration of NOVECRIT stimulated erythropoiesis, yielding elevated hematocrit for at least 14 days. For each data set, the order of histograms from left to right is: Group 1, Group 2, Group 3, Group 4, and Group 5.

FIG. 33 provides an illustrative experimental design for studying the effects of RNAs encoding BMP7 variants for the prevention and treatment of diabetic nephropathy.

FIG. 34 depicts BMP7 protein levels in rats treated with RNAs encoding BMP7 variants as described in Example 42. Error bars indicate SEM (n=6).

FIG. 35 depicts the urine volume, urine albumin, and urine creatinine levels in rats treated with RNAs encoding BMP7 variants as described in Example 42. For each data set, the order of histograms from left to right is: urine volume, urine creatinine, and urine albumin.

FIG. 36 depicts the effect of RNAs encoding BMP7 variants in treating diabetic nephropathy as described in Example 42. For each data set, the order of histograms from left to right is: Group 6—vehicle and Group 7—FTB-2 (BMP7 variant A).

FIG. 37, panels A-I, depict the results of gene-expression analysis of human epidermal keratinocytes transfected with RNA encoding BDNF, BMP-2, BMP-6, IL-2, IL-6, IL-15, IL-22, LIF or FGF-21.

FIG. 38 depicts the results of gene-expression analysis of human epidermal keratinocytes transfected with RNA encoding IL-15 or IL-15 and IL-15RA.

FIG. 39 depicts the serum levels of FGF21, IL15, IL6, IL22, and Novepoietin following a single intradermal injection of various RNAs encoding these proteins as described in Example 45. Three rats were analyzed for each RNA tested.

FIG. 40 depicts the results of the wound-healing assay described in Example 46. Four scratch locations were measured for each well, and two wells were measured for each condition. Error bars indicate standard deviation.

FIG. 41 depicts gene editing of primary human epidermal keratinocytes, as described in Example 47.

FIG. 42 depicts gene correction of primary human epidermal keratinocytes, as described in Example 47.

FIG. 43 depicts gene editing of primary human epidermal keratinocytes, as described in Example 47.

FIG. 44 depicts IL10 production in COLO 205 human colorectal adenocarcinoma cells. COLO 205 cells were plated at a density of 20,000 cells/well in a 24 well plate in 0.5 mL RPMI1640+10% FBS per well. The next day, cells were either (i) transfected with 0.4 μg RNA encoding GFP, as described in example (Example 3), (ii) transfected with 0.4 μg RNA encoding IL22, as described in example (Example 3), (iii) exposed to cell culture medium taken from human epidermal keratinocytes transfected with RNA encoding IL22, (iv) exposed to cell culture medium taken from untransfected human epidermal keratinocytes, (v) exposed to recombinant human IL22 (782-IL-010; R&D Systems). 48h later, IL10 levels in the culture medium were measured via ELISA (D1000B; R&D Systems). IL22 levels for sample (iii) were determined via ELISA (D2200; R&D Systems) prior to the experiment, and the same volumes of medium were used for condition (iv).

FIG. 45 depicts a BMP7 activity assay involving the measurement of alkaline phosphatase activity in ATDC 5 cells following transfection with RNA encoding BMP7 (variant A) or exposure to recombinant human BMP7. ATDC 5 cells were plated at a density of 20,000 cells/well in a 24 well plate in DMEM/F12+5% FBS. The next day, the cells were serum starved by changing the medium to DMEM/F12+2% FBS. The following day, the cells were either (i) transfected with RNA encoding BMP7 or (ii) exposed to recombinant human BMP7 (54-BP-010, R&D Systems). Three days later, intracellular alkaline phosphatase activity was measured (ab83369; Abcam).

FIG. 46 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were transfected with 2 μg RNA encoding the indicated gene-editing proteins (1 μg L and 1 μg R). DNA was harvested after 48h and analyzed for gene editing (A/B indicates RIBOSLICE_A L, RIBOSLICE_B R; RIBOSLICE A indicates repeat sequences comprising the sequence: GHGG, HG, GHGG, HG, etc.; RIBOSLICE B indicates repeat sequences comprising the sequence: HG, GHGG, HG, GHGG, etc.; L targets the sequence: TGCCTGGTCCCTGTCTCCCT (SEQ ID NO: 615); R targets the sequence: TGTCTTCTGGGCAGCATCTC (SEQ ID NO: 616); a target sequence is approximately 75 bp from A1AT [SERPINA1] start codon). “TAL” indicates a control TALEN directed to the target sequence.

FIG. 47 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were transfected with 2 μg RNA encoding the indicated gene-editing proteins (1 μg L and 1 μg R). DNA was harvested after 48h and analyzed for gene editing (NB indicates RIBOSLICE_A L, RIBOSLICE_B R; RIBOSLICE A indicates repeat sequences comprising the sequence: GHGG, HG, GHGG, HG, etc.; RIBOSLICE B indicates repeat sequences comprising the sequence: HG, GHGG, HG, GHGG, etc.; L targets the sequence: TATTCCCGGGCTCCCAGGCA (SEQ ID NO: 622); R targets the sequence: TCTCCTGGCCTTCCTGCCTC (SEQ ID NO: 612); a target sequence is near the end of exon 73 of COL7A1). “TAL” indicates a control TALEN directed to the target sequence.

FIG. 48 depicts the results of an experiment in which 50,000 primary human neonatal epidermal keratinocytes (HEKn) (animal-protein free) were transfected with 2 μg RNA encoding the indicated gene-editing proteins. DNA was harvested after 48h and analyzed for gene editing (“Neg” indicates untreated HEKn DNA; “WT” indicates wild type FokI; “EA” indicates enhanced activity FokI (S35P and K58E); “Het” indicates heterodimer (L: Q103E, N113D, I116L, R: E107K, H154R, I155K); “EA/Het” indicates both EA and Het; L targets the sequence: TATTCCCGGGCTCCCAGGCA (SEQ ID NO: 622); R targets the sequence: TCTCCTGGCCTTCCTGCCTC (SEQ ID NO: 612); a target sequence is near the end of exon 73 of C0L7A1).

FIG. 49 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) per well of a 6-well plate were transfected with 2 μg RNA encoding hGDF15. The culture medium was analyzed for GDF15 at the indicated time after transfection by ELISA (R&D DGD150).

FIG. 50 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) per well of a 6-well plate were transfected with 2 ug RNA encoding the indicated protein. The culture medium was analyzed for IFKB at the indicated time after transfection by ELISA (Abcam ab176644). For each time point, the histograms indicate (left to right): IFKB WT, IFKB S32A, S36A, and Neg.

FIG. 51 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) per well of a 6-well plate were transfected with 2 μg RNA encoding the indicated protein. Images were taken 24 h after transfection.

FIG. 52 depicts the results of an experiment in which 20,000 primary human neonatal epidermal keratinocytes (animal-protein free) per well of a 24-well plate were transfected with 0.2 μg RNA encoding SOD3. Cells were fixed and stained 24 h after transfection with a 1:100 dilution of NBP2-38493 (Novus) rabbit anti human SOD3 primary antibody and a 1:1000 dilution of 488 donkey anti-rabbit secondary antibody. “BF” indicates brightfield and “FL” indicates fluorescence.

FIG. 53 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4, grey curve (bottom)) or with vehicle only (Group 3, black curve (top)). ALT serum levels were measured at the indicated times.

FIG. 54 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4, grey curve (bottom)) or with vehicle only (Group 3, black curve (top)). AST serum levels were measured at the indicated times.

FIG. 55 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4, grey curve (top)) or with vehicle only (Group 3, black curve (bottom)). Total cholesterol serum levels were measured at the indicated times.

FIG. 56 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4, grey (bottom at day 7)) or with vehicle only (Group 3, black (top at day 7)). Glucose serum levels were measured at the indicated times.

FIG. 57 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4, grey (top)) or with vehicle only (Group 3, black (bottom)). Triglycerides serum levels were measured at the indicated times.

FIG. 58 depicts the results of an experiment in which ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (treatment group) or with vehicle only (control group). % change of ALT, AST, Total cholesterol, Glucose, and Triglycerides serum levels in treatment group vs control group are shown.

FIG. 59 depicts the results of an experiment in which lean Sprague-Dawley rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 2) or with vehicle only (Group 1) and ZDF rats were administered RNA encoding hGDF15 by intradermal injection on Days 1, 8, and 15 (Group 4) or with vehicle only (Group 3). GDF15 serum levels were measured at the indicated times. For each timepoint, the histograms indicate (left to right) Group 1, Group 2, Group 3, and Group 4.

FIG. 60 depicts the results of an experiment in which 200,000 primary human neonatal epidermal keratinocytes (animal-protein free) per well of a 6-well plate were transfected with 2 μg RNA encoding hESM1. The culture medium was analyzed for ESM1 52 hours after transfection by ELISA (Abcam ab213776).

FIG. 61 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were transfected with 2 μg RNA encoding the HBB exon 1 TALEN L and HBB exon 1 TALEN R gene-editing proteins (1 μg each). DNA was harvested after 48h and analyzed for gene editing (T7E1 assay; forward primer: gccaaggacaggtacggctgtcatc (SEQ ID NO: 627); reverse primer: cttgccatgagccttcaccttagggttg (SEQ ID NO: 628); product size: 518nt; predicted band sizes: 300nt, 218nt).

FIG. 62 depicts the results of an experiment in which 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were transfected with 2 μg RNA encoding the PD1 exon 1 TALEN L and PD1 exon 1 TALEN R gene-editing proteins (1 μg each). DNA was harvested after 48h and analyzed for gene editing (T7E1 assay; forward primer: tcctctgtctccctgtctctgtctctctctc (SEQ ID NO: 594); reverse primer: ggacttgggccaggggaggag (SEQ ID NO: 595); product size: 612nt; predicted band sizes: 349nt, 263nt).

DETAILED DESCRIPTION OF THE INVENTION

The present invention is based, in part, on the discovery of a safe and effective dosing strategy for nucleic acid drugs, including RNA, such as RNA comprising non-canonical (or “modified”) nucleotides, in humans. The inventors believe this to be the first report of safe and effective dosing of RNA molecules, including those comprising non-canonical nucleotides, in humans. Despite reports in the art that very large doses of RNA molecules are needed for mammalian dosing, and minimal therapeutic effect is achieved despite high dosing (see, e.g. US Patent Publication No. 2013/0245103), the present inventors have surprisingly managed to dose synthetic RNA in a human and achieve significant target protein expression with minimal immunological or other side effects.

In various embodiments, the present invention provides improved doses, formulations, administration and methods of use of nucleic acid drugs, which include RNA, which may contain non-canonical nucleotides (e.g. a residue other than adenine, guanine, thymine, uracil, and cytosine or the standard nucleoside, nucleotide, deoxynucleoside or deoxynucleotide derivatives thereof). In various embodiments, the RNA comprising non-canonical nucleotides leads to the expression of a protein encoded by the RNA, the protein often being one of therapeutic benefit (sometimes called the “target” or “protein of interest”). Further, this expression of therapeutic protein is achieved with minimal or negligible toxicity.

In various aspects, the present invention is based on the surprising discovery of safe and effective doses and administration parameters of nucleic acid drugs for human subjects. Nucleic acid drugs include a dsDNA molecule, a ssDNA molecule, a RNA molecule, a dsRNA molecule, a ssRNA molecule, a plasmid, an oligonucleotide, a synthetic RNA molecule, a miRNA molecule, an mRNA molecule, and an siRNA molecule. In various embodiments, the RNA comprises non-canonical nucleotides.

In some aspects, there is provided a method for delivering a nucleic acid drug, comprising administering an effective dose of a nucleic acid drug to a human subject in need thereof, wherein the nucleic acid drug comprises a synthetic RNA. In various embodiments, the effective dose is an amount sufficient to substantially increase an amount of a protein encoded by the nucleic acid drug in the human subject. For example, when the nucleic acid drug is a synthetic RNA comprising one or more modified nucleotides, the nucleic acid drug may result in higher protein expression than levels obtainable with a nucleic acid drug that does not comprise one or more modified nucleotides (e.g., RNA comprising the canonical nucleotides A, G, U, and C). In some embodiments, the nucleic acid drug results in about a 2-fold, or about a 3-fold, or about a 4-fold, or about a 5-fold, or about a 10-fold, or about a 15-fold, or about a 20-fold, or about a 25-fold, or about a 30-fold, or about a 35-fold, or about a 40-fold, or about a 45-fold, or about a 50-fold, or about a 100-fold increase in protein expression as compared to levels obtainable with a nucleic acid drug that does not comprise one or more modified nucleotides.

In some embodiments, the nucleic acid drug provides a sustained therapeutic effect that is optionally mediated by a sustained expression of target protein. For instance, in some embodiments, the therapeutic effect is present for over about 1 day, or over about 2 days, or over about 3 days, or over about 4 days, or over about 5 days, or over about 6 days, or over about 7 days, or over about 8 days, or over about 9 days, or over about 10 days, or over about 14 days after administration. In some embodiments, this sustained effect obviates the need for, or reduces the amount of, maintenance doses.

In some embodiments, the nucleic acid drug provides a sustained target protein level. For instance, in some embodiments, the target protein is present (e.g. in measurable amounts, e.g. in the serum of a patient to whom the nucleic acid drug has been administered) for over about 1 day, or over about 2 days, or over about 3 days, or over about 4 days, or over about 5 days, or over about 6 days, or over about 7 days, or over about 8 days, or over about 9 days, or over about 10 days, or over about 14 days after administration. In some embodiments, this sustained effect obviates the need for, or reduces the amount of, maintenance doses.

In various embodiments, the nucleic acid drug provides therapeutic action without sustained presence of the nucleic acid drug itself. In some embodiments, the nucleic acid drug is rapidly metabolized, for instance, within about 6 hours, or about 12 hours, or about 18 hours, or about 24 hours, or about 2 days, or about 3 days, or about 4 days, or about 5 days, or about 1 week from administration.

In various embodiments, the effective dose is an amount that substantially avoids cell toxicity in vivo. In various embodiments, the effective dose is an amount that substantially avoids an immune reaction in a human subject. For example, the immune reaction may be an immune response mediated by the innate immune system. Immune response can be monitored using markers known in the art (e.g. cytokines, interferons, TLRs). In some embodiments, the effective dose obviates the need for treatment of the human subject with immune suppressants agents (e.g. B18R) used to moderate the residual toxicity. Accordingly, in some embodiments, the present methods allow for dosing that provides increased protein expression and reduces toxicity.

In some embodiments, the immune response is reduced by about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 99%, about 99.9%, or greater than about 99.9% as compared to the immune response induced by a corresponding unmodified nucleic acid. In some embodiments, upregulation of one or more immune response markers is reduced by about 10%, about 20%, about 30%, about 40%, about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 99%, about 99.9%, or greater than about 99.9% as compared to the upregulation of the one or more immune response markers induced by a corresponding unmodified nucleic acid. In some embodiments, the immune response marker comprises an mRNA or protein product of an interferon gene, including an interferon alpha gene, IFNB1, TLR3, RARRES3, EIF2AK2, STAT1, STAT2, IFIT1, IFIT2, IFIT3, IFIT5, OAS1, OAS2, OAS3, OASL, ISG20 or a fragment, variant, analogue, or family-member thereof. In some embodiments, the immune response marker comprises an mRNA or protein product of an TNF gene, including an TNF alpha gene, TNFRSF1A; TNFRSF1B; LTBR; TNFRSF4; CD40; FAS; TNFRSF6B; CD27; TNFRSF8; TNFRSF9; TNFRSF10A; TNFRSF10B; TNFRSF10C; TNFRSF10D; TNFRSF11A; TNFRSF11B; TNFRSF12A; TNFRSF13B; TNFRSF13C; TNFRSF14; NGFR; TNFRSF17; TNFRSF18; TNFRSF19; TNFRSF21; TNFRSF25; and EDA2R or a fragment, variant, analogue, or family-member thereof. In some embodiments, the immune response marker comprises an mRNA or protein product of an interleukin gene, including an IL-6 gene, IL-1; IL-2; IL-3; IL-4; IL-5; IL-6; IL-7; IL-8 or CXCL8; IL-9; IL-10; IL-11; IL-12; IL-13; IL-14; IL-15; IL-16; IL-17; IL-18; IL-19; IL-20; IL-21; IL-22; IL-23; IL-24; IL-25; IL-26; IL-27; IL-28; IL-29; IL-30; IL-31; IL-32; IL-33; IL-35; IL-36 or a fragment, variant, analogue, or family-member thereof.

In some embodiments, cell death is about 10%, about 25%, about 50%, about 75%, about 85%, about 90%, about 95%, or over about 95% less than the cell death observed with a corresponding unmodified nucleic acid. Moreover, cell death may affect fewer than about 50%, about 40%, about 30%, about 20%, about 10%, about 5%, about 1%, about 0.1%, about 0.01% or fewer than about 0.01% of cells contacted with the modified nucleic acids.

In some embodiments, there is provided a method for expressing a protein of interest in a population of cells in a mammalian subject, comprising administering a non-viral transfection composition comprising an effective dose of a RNA encoding the protein of interest to said cells, the RNA containing one or more non-canonical nucleotides that avoid substantial cellular toxicity, where the transfection composition is administered in an amount that allows for expression of said protein in said cells for at least about five days (e.g. about 5, or about 6, or about 7, about 8, or about 9, or about 10, or about 14 days) without substantial cellular toxicity. In some embodiments, there is provided a method for expressing a protein of interest in a population of cells in a mammalian subject, comprising administering a non-viral transfection composition comprising an effective dose of a RNA encoding the protein of interest to said cells, the RNA containing one or more non-canonical nucleotides that avoid substantial cellular toxicity, where the transfection composition is administered in an amount that allows for expression of said protein in said cells for at least about six hours (e.g. about six hours, or about 12 hours, or about 1 day, or about 2 days, or about 3 days, or about 4 days, or about 5 days) without substantial cellular toxicity.

In some embodiments, the effective dose of the nucleic acid drug, including synthetic RNA, is about 100 ng to about 2000 ng, or about 200 ng to about 1900 ng, or about 300 ng to about 1800 ng, or about 400 ng to about 1700 ng, or about 500 ng to about 1600 ng, or about 600 ng to about 1500 ng, or about 700 ng to about 1400 ng, or about 800 ng to about 1300 ng, or about 900 ng to about 1200 ng, or about 1000 ng to about 1100 ng, or about 500 ng to about 2000 ng, or about 500 ng to about 1500 ng, or about 500 ng to about 1000 ng, or about 1000 ng to about 1500 ng, or about 1000 ng to about 2000 ng, or about 1500 ng to about 2000 ng, or about 100 ng to about 500 ng, or about 200 ng to about 400 ng, or about 10 ng to about 100 ng, or about 20 ng to about 90 ng, or about 30 ng to about 80 ng, or about 40 ng to about 70 ng, or about 50 ng to about 60 ng.

In some embodiments, the effective dose of the nucleic acid drug, including synthetic RNA, is no more than about 50 ng, or about 100 ng, or about 200 ng, or about 300 ng, or about 400 ng, or about 500 ng, or about 600 ng, or about 700 ng, or about 800 ng, or about 900 ng, or about 1000 ng, or about 1100 ng, or about 1200 ng, or about 1300 ng, or about 1400 ng, or about 1500 ng, or about 1600 ng, or about 1700 ng, or about 1800 ng, or about 1900 ng, or about 2000 ng, or about 3000 ng, or about 4000 ng, or about 5000 ng.

In some embodiments, the effective dose of the nucleic acid drug, including synthetic RNA, is about 50 ng, or about 100 ng, or about 200 ng, or about 300 ng, or about 400 ng, or about 500 ng, or about 600 ng, or about 700 ng, or about 800 ng, or about 900 ng, or about 1000 ng, or about 1100 ng, or about 1200 ng, or about 1300 ng, or about 1400 ng, or about 1500 ng, or about 1600 ng, or about 1700 ng, or about 1800 ng, or about 1900 ng, or about 2000 ng, or about 3000 ng, or about 4000 ng, or about 5000 ng.

In some embodiments, the effective dose of the nucleic acid drug, including synthetic RNA, is about 0.028 pmol, or about 0.05 pmol, or about 0.1 μmol, or about 0.2 pmol, or about 0.3 pmol, or about 0.4 μmol, or about 0.5 pmol, or about 0.6 pmol, or about 0.7 μmol, or about 0.8 μmol, or about 0.9 pmol, or about 1.0 pmol, or about 1.2 pmol, or about 1.4 μmol, or about 1.6 pmol, or about 1.8 μmol, or about 2.0 pmol, or about 2.2 pmol, or about 2.4 pmol, or about 2.6 pmol, or about 2.8 μmol, or about 3.0 pmol, or about 3.2 pmol, or about 3.4 μmol, or about 3.6 pmol, or about 3.8 μmol, or about 4.0 pmol, or about 4.2 pmol, or about 4.4 μmol, or about 4.6 pmol, or about 4.8 pmol, or about 5.0 pmol, or about 5.5 pmol, or about 5.7 pmol.

In some embodiments, the nucleic acid drug, including synthetic RNA, is administered at a concentration of about 0.1 nM, or about 0.25 nM, or about 0.5 nM, or about 0.75 nM, or about 1 nM, or about 2.5 nM, or about 5 nM, or about 7.5 nM, or about 10 nM, or about 20 nM, or about 30 nM, or about 40 nM, or about 50 nM, or about 60 nM, or about 70 nM, or about 80 nM, or about 90 nM, or about 100 nM, or about 110 nM, or about 120 nM, or about 150 nM, or about 175 nM, or about 200 nM.

In some embodiments, the effective dose of the nucleic acid drug is about 350 ng/cm², or about 500 ng/cm², or about 750 ng/cm², or about 1000 ng/cm², or about 2000 ng/cm², or about 3000 ng/cm², or about 4000 ng/cm², or about 5000 ng/cm², or about 6000 ng/cm², or about 7000 ng/cm². In other embodiments, the effective dose is less than about 350 ng/cm². In certain embodiments, the effective dose is about 35 ng/cm², or about 50 ng/cm², or about 75 ng/cm², or about 100 ng/cm², or about 150 ng/cm², or about 200 ng/cm², or about 250 ng/cm², or about 300 ng/cm², or about 350 ng/cm².

In some embodiments, the effective dose of the nucleic acid drug is about 35 ng/cm²to about 7000 ng/cm², or about 50 ng/cm²to about 5000 ng/cm², or about 100 ng/cm²to about 3000 ng/cm², or about 500 ng/cm²to about 2000 ng/cm², or about 750 ng/cm²to about 1500 ng/cm², or about 800 ng/cm²to about 1200 ng/cm², or about 900 ng/cm²to about 1100 ng/cm².

In some embodiments, the effective dose of the nucleic acid drug is about 1 picomole/cm², or about 2 picomoles/cm², or about 3 picomoles/cm², or about 4 picomoles/cm², or about 5 picomoles/cm², or about 6 picomoles/cm², or about 7 picomoles/cm², or about 8 picomoles/cm², or about 9 picomoles/cm², or about 10 picomoles/cm², or about 12 picomoles/cm², or about 14 picomoles/cm², or about 16 picomoles/cm², or about 18 picomoles/cm², or about 20 picomoles/cm². In other embodiments, the effective dose is less than about 1 picomole/cm². In certain embodiments, the effective dose is about 0.1 picomoles/cm², or about 0.2 picomoles/cm², or about 0.3 picomoles/cm², or about 0.4 picomoles/cm², or about 0.5 picomoles/cm², or about 0.6 picomoles/cm², or about 0.7 picomoles/cm², or about 0.8 picomoles/cm², or about 0.9 picomoles/cm², or about 1 picomole/cm².

In some embodiments, the effective dose of the nucleic acid drug is about 0.1 picomoles/cm²to about 20 picomoles/cm², or about 0.2 picomoles/cm²to about 15 picomoles/cm², or about 0.5 picomoles/cm²to about 10 picomoles/cm², or about 0.8 picomoles/cm²to about 8 picomoles/cm², or about 1 picomole/cm²to about 5 picomoles/cm², or about 2 picomoles/cm²to about 4 picomoles/cm².

In various embodiments, the nucleic acid drug, including synthetic RNA, is administered in a pharmaceutically acceptable formulation. In various embodiments, the nucleic acid drug, including synthetic RNA, is formulated for one or more of injection and topical administration. By way of example, the nucleic acid drug, including synthetic RNA, may be formulated for injection to a tissue of interest, e.g. a disease site (by way of non-limiting example, a tumor). In various embodiments, injection includes delivery via a patch. In some embodiments, the delivery is mediated by electrical stimulation. In various embodiments, the nucleic acid drug, including synthetic RNA, is formulated for administration to one or more of the epidermis (optionally selected from the stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum, and stratum germinativum), basement membrane, dermis (optionally selected from the papillary region and the reticular region), subcutis, conjunctiva cornea, sclera, iris, lens, corneal limbus, optic nerve, choroid, ciliary body, anterior segment, anterior chamber, and retina. In various embodiments, the nucleic acid drug, including synthetic RNA, is formulated for one or more of subcutaneous injection, intradermal injection, subdermal injection, intramuscular injection, intraocular injection, intravitreal injection, intra-articular injection, intracardiac injection, intravenous injection, epidural injection, intrathecal injection, intraportal injection, intratumoral injection, and topical administration. In various embodiments, the nucleic acid drug, including synthetic RNA, is formulated for intradermal (ID) injection to one or more of the dermis or epidermis. In various embodiments, the nucleic acid drug, including synthetic RNA, is administered in a manner such that it effects one or more of keratinocytes and fibroblasts (e.g. causes these cells to express one or more therapeutic proteins).

Accordingly, the present invention provides various formulations as described herein. Further, in some embodiments, the formulations described herein find use in the various delivery and/or treatment methods of the present invention. For instance, formulations can comprise a vesicle, for instance, a liposome (see Langer, 1990, Science 249:1527-1533; Treat et al., in Liposomes in the Therapy of Infectious Disease and Cancer, Lopez-Berestein and Fidler (eds.), Liss, New York, pp. 353-365 (1989). In various embodiments, the formulation comprises an aqueous suspension of liposomes. Illustrative liposome components are set forth in Table 1, and are given by way of example, and not by way of limitation. In various embodiments, one or more, or two or more, or three or more, or four or more, or five or more of the lipids of Table 1 are combined in a formulation.

TABLE 1

Illustrative Biocompatible Lipids

1
3β-[N-(N′,N′-dimethylaminoethane)-carbamoyl]cholesterol

(DC-Cholesterol)

2
1,2-dioleoyl-3-trimethylammonium-propane (DOTAP/18:1 TAP)

3
N-(4-carboxybenzyl)-N,N-dimethyl-2,3-bis(oleoyloxy)propan-

1-aminium (DOBAQ)

4
1,2-dimyristoyl-3-trimethylammonium-propane (14:0 TAP)

5
1,2-dipalmitoyl-3-trimethylammonium-propane (16:0 TAP)

6
1,2-stearoyl-3-trimethylammonium-propane (18:0 TAP)

7
1,2-dioleoyl-3-dimethylammonium-propane (DODAP/18:1 DAP)

8
1,2-dimyristoyl-3-dimethylammonium-propane (14:0 DAP)

9
1,2-dipalmitoyl-3-dimethylammonium-propane (16:0 DAP)

10
1,2-distearoyl-3-dimethylammonium-propane (18:0 DAP)

11
dimethyldioctadecylammonium (18:0 DDAB)

12
1,2-dilauroyl-sn-glycero-3-ethylphosphocholine (12:0 EthylPC)

13
1,2-dimyristoyl-sn-glycero-3-ethylphosphocholine (14:0 EthylPC)

14
1,2-dimyristoleoyl-sn-glycero-3-ethylphosphocholine (14:1 EthylPC)

15
1,2-dipalmitoyl-sn-glycero-3-ethylphosphocholine (16:0 EthylPC)

16
1,2-distearoyl-sn-glycero-3-ethylphosphocholine (18:0 EthylPC)

17
1,2-dioleoyl-sn-glycero-3-ethylphosphocholine (18:1 EthylPC)

18
1-palmitoyl-2-oleoyl-sn-glycero-3-ethylphosphocholine

(16:1-18:1 EthylPC)

19
1,2-di-O-octadecenyl-3-trimethylammonium propane (DOTMA)

20
N1-[2-((1S)-1-[(3-aminopropyl)amino]-4-[di(3-amino-

propyl)amino]butylcarboxamido)ethyl]-3,4-di[oleyloxy]-benzamide

(MVL5)

21
2,3-dioleyloxy-N-[2-spermine carboxamide]ethyl-N,N-dimethyl-1-

propanammonium trifluoroacetate (DOSPA)

22
1,3-di-oleoyloxy-2-(6-carboxy-spermyl)-propylamid (DOSPER)

23
N-[1-(2,3-dimyristyloxy)propyl]-N,N-dimethyl-N-(2-

hydroxyethyl)ammonium bromide (DMRIE)

24
LIPOFECTAMINE, LIPOFECTAMINE 2000, LIPOFECTAMINE

RNAiMAX, LIPOFECTAMINE 3000, LIPOFECTAMINE

MessengerMAX, TransIT mRNA

25
dioctadecyl amidoglyceryl spermine (DOGS)

26
dioleoyl phosphatidyl ethanolamine (DOPE)

In some embodiments, the liposomes include LIPOFECTAMINE 3000. In some embodiments, the liposomes include one or more lipids described in U.S. Pat. Nos. 4,897,355 or 7,479,573 or in International Patent Publication No. WO/2015/089487, or in Feigner, P. L. et al. (1987) Proc. Natl. Acad. Sci. USA 84:7413-7417, the entire contents of each is incorporated by reference in their entireties).

In some embodiments, the liposome comprises N-[1-(2,3-dioleoyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA). In some embodiments, the liposome comprises dioleoylphosphatidylethanolamine (DOPE).

In one embodiment, the liposomes include one or more polyethylene glycol (PEG) chains, optionally selected from PEG200, PEG300, PEG400, PEG600, PEG800, PEG1000, PEG1500, PEG2000, PEG3000, and PEG4000. In some embodiments, the PEG is PEG2000. In some embodiments, the liposomes include 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) or a derivative thereof.

In some embodiments, the formulation comprises one or more of N-(carbonyl-ethoxypolyethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (MPEG2000-DSPE), fully hydrogenated phosphatidylcholine, cholesterol, LIPOFECTAMINE 3000, a cationic lipid, a polycationic lipid, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-5000] (FA-MPEG5000-DSPE).

In one embodiment, the formulation comprises about 3.2 mg/mL N-(carbonyl-ethoxypolyethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (MPEG2000-DSPE), about 9.6 mg/mL fully hydrogenated phosphatidylcholine, about 3.2 mg/mL cholesterol, about 2 mg/mL ammonium sulfate, and histidine as a buffer, with about 0.27 mg/mL 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-5000] (FA-MPEG5000-DSPE) added to the lipid mixture. In another embodiment, the nucleic acids are complexed by combining 1 μL of LIPOFECTAMINE 3000 per about 1 μg of nucleic acid and incubating at room temperature for at least about 5 minutes. In one embodiment, the LIPOFECTAMINE 3000 is a solution comprising a lipid at a concentration of about 1 mg/mL. In some embodiments, nucleic acids are encapsulated by combining about 10 μg of the liposome formulation per about 1 μg of nucleic acid and incubating at room temperature for about 5 minutes.

In some embodiments, the formulation comprises one or more nanoparticles. In one embodiment, the nanoparticle is a polymeric nanoparticle. In various embodiments, the formulation comprises one or more of a diblock copolymer, a triblock copolymer, a tetrablock copolymer, and a multiblock copolymer. In various embodiments, the formulation comprises one or more of polymeric nanoparticles comprising a polyethylene glycol (PEG)-modified polylactic acid (PLA) diblock copolymer (PLA-PEG) and PEG-polypropylene glycol-PEG-modified PLA-tetrablock copolymer (PLA-PEG-PPG-PEG).

In some embodiments, the formulation comprises one or more lipids that are described in WO/2000/027795, the entire contents of which are hereby incorporated by reference.

In one embodiment, the therapeutic comprises one or more ligands. In another embodiment, the therapeutic comprises at least one of: androgen, CD30 (TNFRSF8), a cell-penetrating peptide, CXCR, estrogen, epidermal growth factor, EGFR, HER2, folate, insulin, insulin-like growth factor-I, interleukin-13, integrin, progesterone, stromal-derived-factor-1, thrombin, vitamin D, and transferrin or a biologically active fragment or variant thereof.

The active compositions of the present invention may include classic pharmaceutical preparations. Administration of these compositions according to the present invention may be via any common route so long as the target tissue is available via that route. This includes oral, nasal, or buccal. Alternatively, administration may be by intradermal, subcutaneous, intramuscular, intraperitoneal, intraportall or intravenous injection, or by direct injection into diseased, e.g. cancer, tissue. The agents disclosed herein may also be administered by catheter systems. Such compositions would normally be administered as pharmaceutically acceptable compositions as described herein.

Administration of the compositions described herein may be, for example, by injection, topical administration, ophthalmic administration and intranasal administration. The injection, in some embodiments, may be linked to an electrical force (e.g. electroporation, including with devices that find use in electrochemotherapy (e.g. CLINIPORATOR, IGEA Srl, Carpi [MO], Italy)). The topical administration may be, but is not limited to, a cream, lotion, ointment, gel, spray, solution and the like. The topical administration may further include a penetration enhancer such as, but not limited to, surfactants, fatty acids, bile salts, chelating agents, non-chelating non-surfactants, polyoxyethylene-9-lauryl ether, polyoxyethylene-20-cetyl ether, fatty acids and/or salts in combination with bile acids and/or salts, sodium salt in combination with lauric acid, capric acid and UDCA, and the like. The topical administration may also include a fragrance, a colorant, a sunscreen, an antibacterial and/or a moisturizer. The compositions described herein may be administered to at least one site such as, but not limited to, forehead, scalp, hair follicles, hair, upper eyelids, lower eyelids, eyebrows, eyelashes, infraorbital area, periorbital areas, temple, nose, nose bridge, cheeks, tongue, nasolabial folds, lips, periobicular areas, jaw line, ears, neck, breast, forearm, upper arm, palm, hand, finger, nails, back, abdomen, sides, buttocks, thigh, calf, feet, toes and the like.

Routes of administration include, for example: intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, oral, sublingual, intracerebral, intravaginal, transdermal, intraportal, rectally, by inhalation, or topically, particularly to the ears, nose, eyes, or skin. In some embodiments, the administering is effected orally or by parenteral injection.

Upon formulation, solutions may be administered in a manner compatible with the dosage formulation and in such amount as is therapeutically effective, as described herein. The formulations may easily be administered in a variety of dosage forms such as injectable solutions, drug release capsules and the like. For parenteral administration in an aqueous solution, for example, the solution generally is suitably buffered and the liquid diluent first rendered isotonic with, for example, sufficient saline or glucose. Such aqueous solutions may be used, for example, for intravenous, intramuscular, subcutaneous and intraperitoneal administration. Preferably, sterile aqueous media are employed as is known to those of skill in the art, particularly in light of the present disclosure.

In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose is administered to a surface area of about 4 mm²to about 150 mm²(e.g. about, or no more than about, 4 mm², or about 5 mm², or about 6 mm², or about 7 mm², or about 8 mm², or about 10 mm², or about 20 mm², or about 50 mm², or about 100 mm², or about 150 mm²). In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose administered to a surface area of no more than about 4 mm², or about 5 mm², or about 6 mm², or about 7 mm², or about 8 mm², or about 10 mm², or about 20 mm², or about 50 mm², or about 100 mm², or about 150 mm². In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose administered to a surface area of about 4 mm², or about 5 mm², or about 6 mm², or about 7 mm², or about 8 mm², or about 10 mm², or about 20 mm², or about 50 mm², or about 100 mm², or about 150 mm².

In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose (weight RNA/surface area of injection) is about 35 ng/cm²to about 7000 ng/cm². In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose (weight RNA/surface area of injection) is no more than about 35 ng/cm², or about 50 ng/cm², or about 75 ng/cm², or about 100 ng/cm², or about 125 ng/cm², or about 150 ng/cm², or about 175 ng/cm², or about 200 ng/cm², or about 225 ng/cm², or about 250 ng/cm², or about 500 ng/cm², or about 1000 ng/cm², or about 2000 ng/cm², or about 5000 ng/cm², or about 7000 ng/cm². In various embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, and/or formulations comprising the same, is administered locally, optionally by one or more of subcutaneous injection, intradermal injection, subdermal injection and intramuscular injection, and the effective dose (weight RNA/surface area of injection) is about 35 ng/cm², or about 50 ng/cm², or about 75 ng/cm², or about 100 ng/cm², or about 125 ng/cm², or about 150 ng/cm², or about 175 ng/cm², or about 200 ng/cm², or about 225 ng/cm², or about 250 ng/cm², or about 500 ng/cm², or about 1000 ng/cm², or about 2000 ng/cm², or about 5000 ng/cm², or about 7000 ng/cm².

Pharmaceutical preparations may additionally comprise delivery reagents (a.k.a. “transfection reagents”, a.k.a. “vehicles”, a.k.a. “delivery vehicles”) and/or excipients. Pharmaceutically acceptable delivery reagents, excipients, and methods of preparation and use thereof, including methods for preparing and administering pharmaceutical preparations to patients (a.k.a. “subjects”) are well known in the art, and are set forth in numerous publications, including, for example, in US Patent Appl. Pub. No. US 2008/0213377, the entirety of which is incorporated herein by reference.

For example, the present compositions can be in the form of pharmaceutically acceptable salts. Such salts include those listed in, for example, J. Pharma. Sci. 66, 2-19 (1977) and The Handbook of Pharmaceutical Salts; Properties, Selection, and Use. P. H. Stahl and C. G. Wermuth (eds.), Verlag, Zurich (Switzerland) 2002, which are hereby incorporated by reference in their entirety. Non-limiting examples of pharmaceutically acceptable salts include: sulfate, citrate, acetate, oxalate, chloride, bromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, camphorsulfonate, pamoate, phenylacetate, trifluoroacetate, acrylate, chlorobenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, methylbenzoate, o-acetoxybenzoate, naphthalene-2-benzoate, isobutyrate, phenylbutyrate, α-hydroxybutyrate, butyne-1,4-dicarboxylate, hexyne-1,4-dicarboxylate, caprate, caprylate, cinnamate, glycollate, heptanoate, hippurate, malate, hydroxymaleate, malonate, mandelate, mesylate, nicotinate, phthalate, teraphthalate, propiolate, propionate, phenylpropionate, sebacate, suberate, p-bromobenzenesulfonate, chlorobenzenesulfonate, ethylsulfonate, 2-hydroxyethylsulfonate, methylsulfonate, naphthalene-1-sulfonate, naphthalene-2-sulfonate, naphthalene-1,5-sulfonate, xylenesulfonate, tartarate salts, hydroxides of alkali metals such as sodium, potassium, and lithium; hydroxides of alkaline earth metal such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, and organic amines, such as unsubstituted or hydroxy-substituted mono-, di-, or tri-alkylamines, dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-OH-lower alkylamines), such as mono-; bis-, or tris-(2-hydroxyethyl)amine, 2-hydroxy-tert-butylamine, or tris-(hydroxymethyl)methylamine, N,N-di-lower alkyl-N-(hydroxyl-lower alkyl)-amines, such as N,N-dimethyl-N-(2-hydroxyethyl)amine or tri-(2-hydroxyethyl)amine; N-methyl-D-glucamine; and amino acids such as arginine, lysine, and the like.

The present pharmaceutical compositions can comprises excipients, including liquids such as water and oils, including those of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like. The pharmaceutical excipients can be, for example, saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea and the like. In addition, auxiliary, stabilizing, thickening, lubricating, and coloring agents can be used. In one embodiment, the pharmaceutically acceptable excipients are sterile when administered to a subject. Suitable pharmaceutical excipients also include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like. Any agent described herein, if desired, can also comprise minor amounts of wetting or emulsifying agents, or pH buffering agents.

Dosage forms suitable for parenteral administration (e.g. subcutaneous, intradermal, subdermal, intramuscular, intravenous, intraperitoneal, intra-articular, and infusion) include, for example, solutions, suspensions, dispersions, emulsions, and the like. They may also be manufactured in the form of sterile solid compositions (e.g. lyophilized composition), which can be dissolved or suspended in sterile injectable medium immediately before use. They may contain, for example, suspending or dispersing agents known in the art.

In some embodiments, the formulations described herein may comprise albumin and a nucleic acid molecule.

In some embodiments, the invention relates to a cosmetic composition. In one embodiment, the cosmetic composition comprises albumin. In another embodiment, the albumin is treated with an ion-exchange resin or charcoal. In yet another embodiment, the cosmetic composition comprises a nucleic acid molecule. In a further embodiment, the cosmetic composition comprises both albumin and a nucleic acid molecule. Still other embodiments are directed to a cosmetic treatment article comprising a cosmetic composition contained in a device configured to deliver the composition to a patient. Still other embodiments are directed to a device configured to deliver a cosmetic composition to a patient. In one embodiment, the nucleic acid molecule encodes a member of the group: elastin, collagen, tyrosinase, melanocortin 1 receptor, keratin, filaggren, an antibody, and hyaluronan synthase or a biologically active fragment, variant, analogue or family member thereof.

In some embodiments, the present invention provides treatment regimens. The inventors have discovered that the doses and administration described herein can produce a substantial protein expression effect quickly (e.g. in about 6, or about 12, or about 24, or about 36, or about 48 hours). Further, these effects can be sustained for about 7 days, or longer. In some embodiments, the present methods provide for administration of a nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, about weekly to about once every 20 weeks.

In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered about weekly, for at least 2 weeks (e.g. 3, or 4, or 5, or 6, or 7, or 8, or 9, or 10 weeks). In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered about every other week for at least one month (e.g. 1, or 2, or 3, or 4, or 5, or 6, or 12 months). In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered monthly or about every other month. In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered is administered for at least two months, or at least 4 months, or at least 6 months, or at least 9 months, or at least one year.

In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered about weekly, or about once every 2 weeks, or about once every 3 weeks, or about once every 4 weeks, or about once every 5 weeks, or about once every 6 weeks, or about once every 7 weeks, or about once every 8 weeks, or about once every 9 weeks, or about once every 10 weeks, or about once every 11 weeks, or about once every 12 weeks, or about once every 13 weeks, or about once every 14 weeks, or about once every 15 weeks, or about once every 20 weeks, or about once every 24 weeks.

In some embodiments, the nucleic acid drug, including RNA comprising one or more non-canonical nucleotides, is administered no more than about weekly, or about once every 2 weeks, or about once every 3 weeks, or about once every 4 weeks, or about once every 5 weeks, or about once every 6 weeks, or about once every 7 weeks, or about once every 8 weeks, or about once every 9 weeks, or about once every 10 weeks, or about once every 11 weeks, or about once every 12 weeks, or about once every 13 weeks, or about once every 14 weeks, or about once every 15 weeks, or about once every 20 weeks, or about 24 weeks.

Certain proteins have long half-lives, and can persist in tissues for several hours, days, weeks, months or years. It has now been discovered that certain methods of treating a patient can result in accumulation of one or more proteins, including, for example, one or more beneficial proteins. Certain embodiments are therefore directed to a method for treating a patient comprising delivering to a patient in a series of doses a nucleic acid encoding one or more proteins. In one embodiment the nucleic acid comprises RNA comprising one or more non-canonical nucleotides. In another embodiment, a first dose is given at a first time-point. In yet another embodiment, a second dose is given at a second time-point. In a further embodiment, the amount of at least one of the one or more proteins in the patient at the second time-point is greater than the amount of said protein at the first time-point. In a still further embodiment, the method results in the accumulation of said protein in the patient.

In various embodiments, the present invention relates to nucleic acid drugs, which, in various embodiments are RNA comprising one or more non-canonical nucleotides. Certain non-canonical nucleotides, when incorporated into RNA molecules, can reduce the toxicity of the RNA molecules, in part, without wishing to be bound by theory, by interfering with binding of proteins that detect exogenous nucleic acids, for example, protein kinase R, Rig-1 and the oligoadenylate synthetase family of proteins. Non-canonical nucleotides that have been reported to reduce the toxicity of RNA molecules when incorporated therein include: pseudouridine, 5-methyluridine, 2-thiouridine, 5-methylcytidine, N6-methyladenosine, and certain combinations thereof. However, the chemical characteristics of non-canonical nucleotides that can enable them to lower the in vivo toxicity of RNA molecules have, until this point, remained unknown. Furthermore, incorporation of large amounts of most non-canonical nucleotides, for example, 5-methyluridine, 2-thiouridine, 5-methylcytidine, and N6-methyladenosine, can reduce the efficiency with which RNA molecules can be translated into protein, limiting the utility of RNA molecules containing these nucleotides in applications that require protein expression. In addition, while pseudouridine can be completely substituted for uridine in RNA molecules without reducing the efficiency with which the synthetic RNA molecules can be translated into protein, in certain situations, for example, when performing frequent, repeated transfections, synthetic RNA molecules containing only adenosine, guanosine, cytidine, and pseudouridine can exhibit excessive toxicity.

It has now been discovered that, and in some embodiments the invention pertains to, RNA molecules containing one or more non-canonical nucleotides that include one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine can be less toxic than synthetic RNA molecules containing only canonical nucleotides, due in part to the ability of substitutions at these positions to interfere with recognition of synthetic RNA molecules by proteins that detect exogenous nucleic acids, and furthermore, that substitutions at these positions can have minimal impact on the efficiency with which the synthetic RNA molecules can be translated into protein, due in part to the lack of interference of substitutions at these positions with base-pairing and base-stacking interactions.

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Examples of non-canonical nucleotides that include one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine include, but are not limited to: 2-thiouridine, 5-azauridine, pseudouridine, 4-thiouridine, 5-methyluridine, 5-methylpseudouridine, 5-aminouridine, 5-aminopseudouridine, 5-hydroxyuridine, 5-hydroxypseudouridine, 5-methoxyuridine, 5-methoxypseudouridine, 5-hydroxymethyluridine, 5-hydroxymethylpseudouridine, 5-carboxyuridine, 5-carboxypseudouridine, 5-formyluridine, 5-formylpseudouridine, 5-methyl-5-azauridine, 5-amino-5-azauridine, 5-hydroxy-5-azauridine, 5-methylpseudouridine, 5-aminopseudouridine, 5-hydroxypseudouridine, 4-thio-5-azauridine, 4-thiopseudouridine, 4-thio-5-methyluridine, 4-thio-5-aminouridine, 4-thio-5-hydroxyuridine, 4-thio-5-methyl-5-azauridine, 4-thio-5-amino-5-azauridine, 4-thio-5-hydroxy-5-azauridine, 4-thio-5-methylpseudouridine, 4-thio-5-aminopseudouridine, 4-thio-5-hydroxypseudouridine, 2-thiocytidine, 5-azacytidine, pseudoisocytidine, N4-methylcytidine, N4-aminocytidine, N4-hydroxycytidine, 5-methylcytidine, 5-aminocytidine, 5-hydroxycytidine, 5-methoxycytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytydine, 5-methyl-5-azacytidine, 5-amino-5-azacytidine, 5-hydroxy-5-azacytidine, 5-methylpseudoisocytidine, 5-aminopseudoisocytidine, 5-hydroxypseudoisocytidine, N4-methyl-5-azacytidine, N4-methylpseudoisocytidine, 2-thio-5-azacytidine, 2-thiopseudoisocytidine, 2-thio-N4-methylcytidine, 2-thio-N4-aminocytidine, 2-thio-N4-hydroxycytidine, 2-thio-5-methylcytidine, 2-thio-5-aminocytidine, 2-thio-5-hydroxycytidine, 2-thio-5-methyl-5-azacytidine, 2-thio-5-amino-5-azacytidine, 2-thio-5-hydroxy-5-azacytidine, 2-thio-5-methylpseudoisocytidine, 2-thio-5-aminopseudoisocytidine, 2-thio-5-hydroxypseudoisocytidine, 2-thio-N4-methyl-5-azacytidine, 2-thio-N4-methylpseudoisocytidine, N4-methyl-5-methylcytidine, N4-methyl-5-aminocytidine, N4-methyl-5-hydroxycytidine, N4-methyl-5-methyl-5-azacytidine, N4-methyl-5-amino-5-azacytidine, N4-methyl-5-hydroxy-5-azacytidine, N4-methyl-5-methylpseudoisocytidine, N4-methyl-5-aminopseudoisocytidine, N4-methyl-5-hydroxypseudoisocytidine, N4-amino-5-azacytidine, N4-aminopseudoisocytidine, N4-amino-5-methylcytidine, N4-amino-5-aminocytidine, N4-amino-5-hydroxycytidine, N4-amino-5-methyl-5-azacytidine, N4-amino-5-amino-5-azacytidine, N4-amino-5-hydroxy-5-azacytidine, N4-amino-5-methylpseudoisocytidine, N4-amino-5-aminopseudoisocytidine, N4-amino-5-hydroxypseudoisocytidine, N4-hydroxy-5-azacytidine, N4-hydroxypseudoisocytidine, N4-hydroxy-5-methylcytidine, N4-hydroxy-5-aminocytidine, N4-hydroxy-5-hydroxycytidine, N4-hydroxy-5-methyl-5-azacytidine, N4-hydroxy-5-amino-5-azacytidine, N4-hydroxy-5-hydroxy-5-azacytidine, N4-hydroxy-5-methylpseudoisocytidine, N4-hydroxy-5-aminopseudoisocytidine, N4-hydroxy-5-hydroxypseudoisocytidine, 2-thio-N4-methyl-5-methylcytidine, 2-thio-N4-methyl-5-aminocytidine, 2-thio-N4-methyl-5-hydroxycytidine, 2-thio-N4-methyl-5-methyl-5-azacytidine, 2-thio-N4-methyl-5-amino-5-azacytidine, 2-thio-N4-methyl-5-hydroxy-5-azacytidine, 2-thio-N4-methyl-5-methylpseudoisocytidine, 2-thio-N4-methyl-5-aminopseudoisocytidine, 2-thio-N4-methyl-5-hydroxypseudoisocytidine, 2-thio-N4-amino-5-azacytidine, 2-thio-N4-aminopseudoisocytidine, 2-thio-N4-amino-5-methylcytidine, 2-thio-N4-amino-5-aminocytidine, 2-thio-N4-amino-5-hydroxycytidine, 2-thio-N4-amino-5-methyl-5-azacytidine, 2-thio-N4-amino-5-amino-5-azacytidine, 2-thio-N4-amino-5-hydroxy-5-azacytidine, 2-thio-N4-amino-5-methylpseudoisocytidine, 2-thio-N4-amino-5-aminopseudoisocytidine, 2-thio-N4-amino-5-hydroxypseudoisocytidine, 2-thio-N4-hydroxy-5-azacytidine, 2-thio-N4-hydroxypseudoisocytidine, 2-thio-N4-hydroxy-5-methylcytidine, N4-hydroxy-5-aminocytidine, 2-thio-N4-hydroxy-5-hydroxycytidine, 2-thio-N4-hydroxy-5-methyl-5-azacytidine, 2-thio-N4-hydroxy-5-amino-5-azacytidine, 2-thio-N4-hydroxy-5-hydroxy-5-azacytidine, 2-thio-N4-hydroxy-5-methylpseudoisocytidine, 2-thio-N4-hydroxy-5-aminopseudoisocytidine, 2-thio-N4-hydroxy-5-hydroxypseudoisocytidine, N6-methyladenosine, N6-aminoadenosine, N6-hydroxyadenosine, 7-deazaadenosine, 8-azaadenosine, N6-methyl-7-deazaadenosine, N6-methyl-8-azaadenosine, 7-deaza-8-azaadenosine, N6-methyl-7-deaza-8-azaadenosine, N6-amino-7-deazaadenosine, N6-amino-8-azaadenosine, N6-amino-7-deaza-8-azaadenosine, N6-hydroxyadenosine, N6-hydroxy-7-deazaadenosine, N6-hydroxy-8-azaadenosine, N6-hydroxy-7-deaza-8-azaadenosine, 6-thioguanosine, 7-deazaguanosine, 8-azaguanosine, 6-thio-7-deazaguanosine, 6-thio-8-azaguanosine, 7-deaza-8-azaguanosine, 6-thio-7-deaza-8-azaguanosine, and 5-methoxyuridine.

In some embodiments, the invention relates to one or more non-canonical nucleotides selected from 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, 5-hydroxyuridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, pseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine. In some embodiments, at least 50%, or at least 55%, or at least 60%, or at least 65%, or at least 70%, or at least 75%, or at least 80%, or at least 85%, or at least 90%, or at least 95%, or 100% of the non-canonical nucleotides are one or more of 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, pseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine.

In some embodiments, at least about 50%, or at least about 55%%, or at least 60%, or at least 65%, or at least 70%, or at least 75%, or at least 80%, or at least 85%, or at least 90%, or at least 95%, or 100% of cytidine residues are non-canonical nucleotides selected from 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine.

In some embodiments, at least about 20%, or about 30%, or about 40%, or about 50%, or at least about 55%, or at least 60%, or at least 65%, or at least 70%, or at least 75%, or at least 80%, or at least 85%, or at least 90%, or at least 95%, or 100% of uridine residues are non-canonical nucleotides selected from 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, pseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridine.

In some embodiments, at least about 10% (e.g. 10%, or about 20%, or about 30%, or about 40%, or about 50%) of guanosine residues are non-canonical nucleotides, and the non-canonical nucleotide is optionally 7-deazaguanosine. In some embodiments, the RNA contains no more than about 50% 7-deazaguanosine in place of guanosine residues.

In some embodiments, the RNA does not contain non-canonical nucleotides in place of adenosine residues.

Note that alternative naming schemes exist for certain non-canonical nucleotides. For example, in certain situations, 5-methylpseudouridine can be referred to as “3-methylpseudouridine” or “N3-methylpseudouridine” or “1-methylpseudouridine” or “N1-methylpseudouridine”.

Nucleotides that contain the prefix “amino” can refer to any nucleotide that contains a nitrogen atom bound to the atom at the stated position of the nucleotide, for example, 5-aminocytidine can refer to 5-aminocytidine, 5-methylaminocytidine, and 5-nitrocytidine. Similarly, nucleotides that contain the prefix “methyl” can refer to any nucleotide that contains a carbon atom bound to the atom at the stated position of the nucleotide, for example, 5-methylcytidine can refer to 5-methylcytidine, 5-ethylcytidine, and 5-hydroxymethylcytidine, nucleotides that contain the prefix “thio” can refer to any nucleotide that contains a sulfur atom bound to the atom at the given position of the nucleotide, and nucleotides that contain the prefix “hydroxy” can refer to any nucleotide that contains an oxygen atom bound to the atom at the given position of the nucleotide, for example, 5-hydroxyuridine can refer to 5-hydroxyuridine and uridine with a methyl group bound to an oxygen atom, wherein the oxygen atom is bound to the atom at the 5C position of the uridine.

Certain embodiments are therefore directed to RNA comprising one or more non-canonical nucleotides, wherein the RNA molecule contains one or more nucleotides that includes one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. Other embodiments are directed to a therapeutic, wherein the therapeutic contains one or more RNA molecules comprising one or more non-canonical nucleotides, and wherein the one or more RNA molecules comprising one or more non-canonical nucleotides contains one or more nucleotides that includes one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. In one embodiment, the therapeutic comprises a transfection reagent. In another embodiment, the transfection reagent comprises a cationic lipid, liposome or micelle. In still another embodiment, the liposome or micelle comprises folate and the therapeutic composition has anti-cancer activity. In another embodiment, the one or more nucleotides includes at least one of pseudouridine, 2-thiouridine, 4-thiouridine, 5-azauridine, 5-hydroxyuridine, 5-methyluridine, 5-aminouridine, 2-thiopseudouridine, 4-thiopseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-aminopseudouridine, pseudoisocytidine, N4-methylcytidine, 2-thiocytidine, 5-azacytidine, 5-hydroxycytidine, 5-aminocytidine, 5-methylcytidine, N4-methylpseudoisocytidine, 2-thiopseudoisocytidine, 5-hydroxypseudoisocytidine, 5-aminopseudoisocytidine, 5-methylpseudoisocytidine, 7-deazaadenosine, 7-deazaguanosine, 6-thioguanosine, and 6-thio-7-deazaguanosine. In another embodiment, the one or more nucleotides includes at least one of pseudouridine, 2-thiouridine, 4-thiouridine, 5-azauridine, 5-hydroxyuridine, 5-methyluridine, 5-aminouridine, 2-thiopseudouridine, 4-thiopseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, and 5-aminopseudouridine and at least one of pseudoisocytidine, N4-methylcytidine, 2-thiocytidine, 5-azacytidine, 5-hydroxycytidine, 5-aminocytidine, 5-methylcytidine, N4-methylpseudoisocytidine, 2-thiopseudoisocytidine, 5-hydroxypseudoisocytidine, 5-aminopseudoisocytidine, and 5-methylpseudoisocytidine. In still another embodiment, the one or more nucleotides include at least one of pseudouridine, 2-thiouridine, 4-thiouridine, 5-azauridine, 5-hydroxyuridine, 5-methyluridine, 5-aminouridine, 2-thiopseudouridine, 4-thiopseudouridine, 5-hydroxypseudouridine, and 5-methylpseudouridine, 5-aminopseudouridine and at least one of pseudoisocytidine, N4-methylcytidine, 2-thiocytidine, 5-azacytidine, 5-hydroxycytidine, 5-aminocytidine, 5-methylcytidine, N4-methylpseudoisocytidine, 2-thiopseudoisocytidine, 5-hydroxypseudoisocytidine, 5-aminopseudoisocytidine, and 5-methylpseudoisocytidine and at least one of 7-deazaguanosine, 6-thioguanosine, 6-thio-7-deazaguanosine, and 5-methoxyuridine. In yet another embodiment, the one or more nucleotides includes: 5-methylcytidine and 7-deazaguanosine. In another embodiment, the one or more nucleotides also includes pseudouridine or 4-thiouridine or 5-methyluridine or 5-aminouridine or 4-thiopseudouridine or 5-methylpseudouridine or 5-aminopseudouridine. In a still another embodiment, the one or more nucleotides also includes 7-deazaadenosine. In another embodiment, the one or more nucleotides includes: pseudoisocytidine and 7-deazaguanosine and 4-thiouridine. In yet another embodiment, the one or more nucleotides includes: pseudoisocytidine or 7-deazaguanosine and pseudouridine. In still another embodiment, the one or more nucleotides includes: 5-methyluridine and 5-methylcytidine and 7-deazaguanosine. In a further embodiment, the one or more nucleotides includes: pseudouridine or 5-methylpseudouridine and 5-methylcytidine and 7-deazaguanosine. In another embodiment, the one or more nucleotides includes: pseudoisocytidine and 7-deazaguanosine and pseudouridine. In one embodiment, the RNA comprising one or more non-canonical nucleotides is present in vivo.

Certain non-canonical nucleotides can be incorporated more efficiently than other non-canonical nucleotides into RNA molecules by RNA polymerases that are commonly used for in vitro transcription, due in part to the tendency of these certain non-canonical nucleotides to participate in standard base-pairing interactions and base-stacking interactions, and to interact with the RNA polymerase in a manner similar to that in which the corresponding canonical nucleotide interacts with the RNA polymerase. As a result, certain nucleotide mixtures containing one or more non-canonical nucleotides can be beneficial in part because in vitro-transcription reactions containing these nucleotide mixtures can yield a large quantity of RNA. Certain embodiments are therefore directed to a nucleotide mixture containing one or more nucleotides that includes one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. Nucleotide mixtures include, but are not limited to (numbers preceding each nucleotide indicate an exemplary fraction of the non-canonical nucleotide triphosphate in an in vitro-transcription reaction, for example, 0.2 pseudoisocytidine refers to a reaction containing adenosine-5′-triphosphate, guanosine-5′-triphosphate, uridine-5′-triphosphate, cytidine-5′-triphosphate, and pseudoisocytidine-5′-triphosphate, wherein pseudoisocytidine-5′-triphosphate is present in the reaction at an amount approximately equal to 0.2 times the total amount of pseudoisocytidine-5′-triphosphate+cytidine-5′-triphosphate that is present in the reaction, with amounts measured either on a molar or mass basis, and wherein more than one number preceding a nucleoside indicates a range of exemplary fractions): 1.0 pseudouridine, 0.1-0.8 2-thiouridine, 0.1-0.8 5-methyluridine, 0.2-1.0 5-hydroxyuridine, 0.2-1.0 5-methoxyuridine, 0.1-1.0 5-aminouridine, 0.1-1.0 4-thiouridine, 0.1-1.0 2-thiopseudouridine, 0.1-1.0 4-thiopseudouridine, 0.1-1.0 5-hydroxypseudouridine, 0.2-1 5-methylpseudouridine, 0.2-1.0 5-methoxypseudouridine, 0.1-1.0 5-aminopseudouridine, 0.2-1.0 2-thiocytidine, 0.1-0.8 pseudoisocytidine, 0.2-1.0 5-methylcytidine, 0.2-1.0 5-hydroxycytidine, 0.2-1.0 5-hydroxymethylcytidine, 0.2-1.0 5-methoxycytidine, 0.1-1.0 5-aminocytidine, 0.2-1.0 N4-methylcytidine, 0.2-1.0 5-methylpseudoisocytidine, 0.2-1.0 5-hydroxypseudoisocytidine, 0.2-1.0 5-aminopseudoisocytidine, 0.2-1.0 N4-methylpseudoisocytidine, 0.2-1.0 2-thiopseudoisocytidine, 0.2-1.0 7-deazaguanosine, 0.2-1.0 6-thioguanosine, 0.2-1.0 6-thio-7-deazaguanosine, 0.2-1.0 8-azaguanosine, 0.2-1.0 7-deaza-8-azaguanosine, 0.2-1.0 6-thio-8-azaguanosine, 0.1-0.5 7-deazaadenosine, and 0.1-0.5 N6-methyladenosine.

In various embodiments, the RNA comprising one or more non-canonical nucleotides composition or synthetic polynucleotide composition (e.g., which may be prepared by in vitro transcription) contains substantially or entirely the canonical nucleotide at positions having adenine or “A” in the genetic code. The term “substantially” in this context refers to at least 90%. In these embodiments, the RNA composition or synthetic polynucleotide composition may further contain (e.g., consist of) 7-deazaguanosine at positions with “G” in the genetic code as well as the corresponding canonical nucleotide “G”, and the canonical and non-canonical nucleotide at positions with G may be in the range of 5:1 to 1:5, or in some embodiments in the range of 2:1 to 1:2. In these embodiments, the RNA composition or synthetic polynucleotide composition may further contain (e.g., consist of) one or more (e.g., two, three or four) of 5-hydroxycytidine, 5-methylcytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine at positions with “C” in the genetic code as well as the canonical nucleotide “C”, and the canonical and non-canonical nucleotide at positions with C may be in the range of 5:1 to 1:5, or in some embodiments in the range of 2:1 to 1:2. In some embodiments, the level of non-canonical nucleotide at positions of “C” are as described in the preceding paragraph. In these embodiments, the RNA composition or synthetic polynucleotide composition may further contain (e.g., consist of) one or more (e.g., two, three, or four) of 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, 5-methoxyuridine, pseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-formylpseudouridine, and 5-methoxypseudouridineat positions with “U” in the genetic code as well as the canonical nucleotide “U”, and the canonical and non-canonical nucleotide at positions with “U” may be in the range of 5:1 to 1:5, or in some embodiments in the range of 2:1 to 1:2. In some embodiments, the level of non-canonical nucleotide at positions of “U” are as described in the preceding paragraph.

It has now been discovered that combining certain non-canonical nucleotides can be beneficial in part because the contribution of non-canonical nucleotides to lowering the toxicity of RNA molecules can be additive. Certain embodiments are therefore directed to a nucleotide mixture, wherein the nucleotide mixture contains more than one of the non-canonical nucleotides listed above, for example, the nucleotide mixture contains both pseudoisocytidine and 7-deazaguanosine or the nucleotide mixture contains both N4-methylcytidine and 7-deazaguanosine, etc. In one embodiment, the nucleotide mixture contains more than one of the non-canonical nucleotides listed above, and each of the non-canonical nucleotides is present in the mixture at the fraction listed above, for example, the nucleotide mixture contains 0.1-0.8 pseudoisocytidine and 0.2-1.0 7-deazaguanosine or the nucleotide mixture contains 0.2-1.0 N4-methylcytidine and 0.2-1.0 7-deazaguanosine, etc.

In certain situations, for example, when it may not be necessary or desirable to maximize the yield of an in vitro-transcription reaction, nucleotide fractions other than those given above may be used. The exemplary fractions and ranges of fractions listed above relate to nucleotide-triphosphate solutions of typical purity (greater than 90% purity). Larger fractions of these and other nucleotides can be used by using nucleotide-triphosphate solutions of greater purity, for example, greater than about 95% purity or greater than about 98% purity or greater than about 99% purity or greater than about 99.5% purity, which can be achieved, for example, by purifying the nucleotide triphosphate solution using existing chemical-purification technologies such as high-pressure liquid chromatography (HPLC) or by other means. In one embodiment, nucleotides with multiple isomers are purified to enrich the desired isomer.

Other embodiments are directed to a method for inducing a cell in vivo to express a protein of interest by contacting the cell with a RNA molecule that contains one or more non-canonical nucleotides that includes one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. Still other embodiments are directed to a method for transfecting, reprogramming, and/or gene-editing a cell in vivo by contacting the cell with a RNA molecule that contains one or more non-canonical nucleotides that includes one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. In one embodiment, the RNA molecule is produced by in vitro transcription. In one embodiment, the RNA molecule encodes one or more reprogramming factors. In another embodiment, the one or more reprogramming factors includes Oct4 protein. In another embodiment, the cell is also contacted with a RNA molecule that encodes Sox2 protein. In yet another embodiment, the cell is also contacted with a RNA molecule that encodes Klf4 protein. In yet another embodiment, the cell is also contacted with a RNA molecule that encodes c-Myc protein. In yet another embodiment, the cell is also contacted with a RNA molecule that encodes Lin28 protein.

Enzymes such as T7 RNA polymerase may preferentially incorporate canonical nucleotides in an in vitro-transcription reaction containing both canonical and non-canonical nucleotides. As a result, an in vitro-transcription reaction containing a certain fraction of a non-canonical nucleotide may yield RNA containing a different, often lower, fraction of the non-canonical nucleotide than the fraction at which the non-canonical nucleotide was present in the reaction. In certain embodiments, references to nucleotide incorporation fractions (for example, “a synthetic RNA molecule containing 50% pseudoisocytidine” or “0.1-0.8 pseudoisocytidine”) therefore can refer both to RNA molecules containing the stated fraction of the nucleotide, and to RNA molecules synthesized in a reaction containing the stated fraction of the nucleotide (or nucleotide derivative, for example, nucleotide-triphosphate), even though such a reaction may yield RNA containing a different fraction of the nucleotide than the fraction at which the non-canonical nucleotide was present in the reaction.

Different nucleotide sequences can encode the same protein by utilizing alternative codons. In certain embodiments, references to nucleotide incorporation fractions therefore can refer both to RNA molecules containing the stated fraction of the nucleotide, and to RNA molecules encoding the same protein as a different RNA molecule, wherein the different RNA molecule contains the stated fraction of the nucleotide.

Certain embodiments are directed to a kit containing one or more materials needed to practice the present invention. In one embodiment, the kit contains one or more synthetic RNA molecules. In one embodiment, the kit contains one or more synthetic RNA molecules that encode one or more reprogramming factors and/or gene-editing proteins. In another embodiment, the one or more synthetic RNA molecules contain one or more non-canonical nucleotides that include one or more substitutions at the 2C and/or 4C and/or 5C positions in the case of a pyrimidine or the 6C and/or 7N and/or 8C positions in the case of a purine. In another embodiment, the kit contains one or more of: a transfection medium, a transfection reagent, a complexation medium, and a matrix solution. In one embodiment, the matrix solution contains fibronectin and/or vitronectin or recombinant fibronectin and/or recombinant vitronectin. In one embodiment, one or more of the components of the kit are present as a plurality of aliquots. In one embodiment, the kit contains aliquots of nucleic acid transfection-reagent complexes. In another embodiment, the kit contains aliquots of nucleic acid transfection-reagent complexes that are provided in a solid form, for example, as frozen or freeze-dried pellets. In yet another embodiment, the kit contains aliquots of medium, wherein each aliquot contains transfection reagent-nucleic acid complexes that are stabilized either by chemical treatment or by freezing.

Transfection, in general, and reprogramming, in particular, can be difficult and time-consuming techniques that can be repetitive and prone to error. However, these techniques are often performed manually due to the lack of automated transfection equipment. Certain embodiments are therefore directed to a system that can transfect, reprogram, and/or gene-edit cells in vivo in an automated or semi-automated manner.

It has now been discovered that the non-canonical nucleotide members of the 5-methylcytidine de-methylation pathway, when incorporated into synthetic RNA, can increase the efficiency with which the synthetic RNA can be translated into protein in vivo, and can decrease the toxicity of the synthetic RNA in vivo. These non-canonical nucleotides include, for example: 5-methylcytidine, 5-hydroxymethylcytidine, 5-formylcytidine, and 5-carboxycytidine (a.k.a. “cytidine-5-carboxylic acid”). Certain embodiments are therefore directed to a nucleic acid. In some embodiments, the nucleic acid is present in vivo. In one embodiment, the nucleic acid is a synthetic RNA molecule. In another embodiment, the nucleic acid comprises one or more non-canonical nucleotides. In one embodiment, the nucleic acid comprises one or more non-canonical nucleotide members of the 5-methylcytidine de-methylation pathway. In another embodiment, the nucleic acid comprises at least one of: 5-methylcytidine, 5-hydroxymethylcytidine, 5-formylcytidine, and 5-carboxycytidine or a derivative thereof. In a further embodiment, the nucleic acid comprises at least one of: pseudouridine, 5-methylpseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-methylcytidine, 5-hydroxymethylcytidine, N4-methylcytidine, N4-acetylcytidine, and 7-deazaguanosine or a derivative thereof.

5-Methylcytidine De-Methylation Pathway

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Certain embodiments are directed to a protein. Other embodiments are directed to a nucleic acid that encodes a protein. In one embodiment, the protein is a protein of interest. In another embodiment, the protein is selected from: a reprogramming protein and a gene-editing protein. In one embodiment, the nucleic acid is a plasmid. In another embodiment, the nucleic acid is present in a virus or viral vector. In a further embodiment, the virus or viral vector is replication incompetent. In a still further embodiment, the virus or viral vector is replication competent. In one embodiment, the virus or viral vector includes at least one of: an adenovirus, a retrovirus, a lentivirus, a herpes virus, an adeno-associated virus or a natural or engineered variant thereof, and an engineered virus.

It has also been discovered that certain combinations of non-canonical nucleotides can be particularly effective at increasing the efficiency with which synthetic RNA can be translated into protein in vivo, and decreasing the toxicity of synthetic RNA in vivo, for example, the combinations: 5-methyluridine and 5-methylcytidine, 5-hydroxyuridine and 5-methylcytidine, 5-hydroxyuridine and 5-hydroxymethylcytidine, 5-methyluridine and 7-deazaguanosine, 5-methylcytidine and 7-deazaguanosine, 5-methyluridine, 5-methylcytidine, and 7-deazaguanosine, and 5-methyluridine, 5-hydroxymethylcytidine, and 7-deazaguanosine. Certain embodiments are therefore directed to a nucleic acid comprising at least two of: 5-methyluridine, 5-methylcytidine, 5-hydroxymethylcytidine, and 7-deazaguanosine or one or more derivatives thereof. Other embodiments are directed to a nucleic acid comprising at least three of: 5-methyluridine, 5-methylcytidine, 5-hydroxymethylcytidine, and 7-deazaguanosine or one or more derivatives thereof. Other embodiments are directed to a nucleic acid comprising all of: 5-methyluridine, 5-methylcytidine, 5-hydroxymethylcytidine, and 7-deazaguanosine or one or more derivatives thereof. In one embodiment, the nucleic acid comprises one or more 5-methyluridine residues, one or more 5-methylcytidine residues, and one or more 7-deazaguanosine residues or one or more 5-methyluridine residues, one or more 5-hydroxymethylcytidine residues, and one or more 7-deazaguanosine residues.

It has been further discovered that synthetic RNA molecules containing certain fractions of certain non-canonical nucleotides and combinations thereof can exhibit particularly high translation efficiency and low toxicity in vivo. Certain embodiments are therefore directed to a nucleic acid comprising at least one of: one or more uridine residues, one or more cytidine residues, and one or more guanosine residues, and comprising one or more non-canonical nucleotides. In one embodiment, between about 20% and about 80% of the uridine residues are 5-methyluridine residues. In another embodiment, between about 30% and about 50% of the uridine residues are 5-methyluridine residues. In a further embodiment, about 40% of the uridine residues are 5-methyluridine residues. In one embodiment, between about 60% and about 80% of the cytidine residues are 5-methylcytidine residues. In another embodiment, between about 80% and about 100% of the cytidine residues are 5-methylcytidine residues. In a further embodiment, about 100% of the cytidine residues are 5-methylcytidine residues. In a still further embodiment, between about 20% and about 100% of the cytidine residues are 5-hydroxymethylcytidine residues. In one embodiment, between about 20% and about 80% of the guanosine residues are 7-deazaguanosine residues. In another embodiment, between about 40% and about 60% of the guanosine residues are 7-deazaguanosine residues. In a further embodiment, about 50% of the guanosine residues are 7-deazaguanosine residues. In one embodiment, between about 20% and about 80% or between about 30% and about 60% or about 40% of the cytidine residues are N4-methylcytidine and/or N4-acetylcytidine residues. In another embodiment, each cytidine residue is a 5-methylcytidine residue. In a further embodiment, about 100% of the cytidine residues are 5-methylcytidine residues and/or 5-hydroxymethylcytidine residues and/or N4-methylcytidine residues and/or N4-acetylcytidine residues and/or one or more derivatives thereof. In a still further embodiment, about 40% of the uridine residues are 5-methyluridine residues, between about 20% and about 100% of the cytidine residues are N4-methylcytidine and/or N4-acetylcytidine residues, and about 50% of the guanosine residues are 7-deazaguanosine residues. In one embodiment, about 40% of the uridine residues are 5-methyluridine residues and about 100% of the cytidine residues are 5-methylcytidine residues. In another embodiment, about 40% of the uridine residues are 5-methyluridine residues and about 50% of the guanosine residues are 7-deazaguanosine residues. In a further embodiment, about 100% of the cytidine residues are 5-methylcytidine residues and about 50% of the guanosine residues are 7-deazaguanosine residues. In a further embodiment, about 100% of the uridine residues are 5-hydroxyuridine residues. In one embodiment, about 40% of the uridine residues are 5-methyluridine residues, about 100% of the cytidine residues are 5-methylcytidine residues, and about 50% of the guanosine residues are 7-deazaguanosine residues. In another embodiment, about 40% of the uridine residues are 5-methyluridine residues, between about 20% and about 100% of the cytidine residues are 5-hydroxymethylcytidine residues, and about 50% of the guanosine residues are 7-deazaguanosine residues. In some embodiments, less than 100% of the cytidine residues are 5-methylcytidine residues. In other embodiments, less than 100% of the cytidine residues are 5-hydroxymethylcytidine residues. In one embodiment, each uridine residue in the synthetic RNA molecule is a pseudouridine residue or a 5-methylpseudouridine residue. In another embodiment, about 100% of the uridine residues are pseudouridine residues and/or 5-methylpseudouridine residues. In a further embodiment, about 100% of the uridine residues are pseudouridine residues and/or 5-methylpseudouridine residues, about 100% of the cytidine residues are 5-methylcytidine residues, and about 50% of the guanosine residues are 7-deazaguanosine residues.

Other non-canonical nucleotides that can be used in place of or in combination with 5-methyluridine include, but are not limited to: pseudouridine, 5-hydroxyuridine, 5-hydroxypseudouridine, 5-methoxyuridine, 5-methoxypseudouridine, 5-carboxyuridine, 5-carboxypseudouridine, 5-formyluridine, 5-formylpseudouridine, 5-hydroxymethyluridine, 5-hydroxymethylpseudouridine, and 5-methylpseudouridine (a.k.a. “1-methylpseudouridine”, a.k.a. “N1-methylpseudouridine”) or one or more derivatives thereof. Other non-canonical nucleotides that can be used in place of or in combination with 5-methylcytidine and/or 5-hydroxymethylcytidine include, but are not limited to: pseudoisocytidine, 5-methylpseudoisocytidine, 5-hydroxymethylcytidine, 5-formylcytidine, 5-carboxycytidine, 5-methoxycytidine, N4-methylcytidine, N4-acetylcytidine or one or more derivatives thereof. In certain embodiments, for example, when performing only a single transfection, injection or delivery or when the cells, tissue, organ or patient being transfected, injected or delivered to are not particularly sensitive to transfection-associated toxicity or innate-immune signaling, the fractions of non-canonical nucleotides can be reduced. Reducing the fraction of non-canonical nucleotides can be beneficial, in part, because reducing the fraction of non-canonical nucleotides can reduce the cost of the nucleic acid. In certain situations, for example, when minimal immunogenicity of the nucleic acid is desired, the fractions of non-canonical nucleotides can be increased.

Enzymes such as T7 RNA polymerase may preferentially incorporate canonical nucleotides in an in vitro-transcription reaction containing both canonical and non-canonical nucleotides. As a result, an in vitro-transcription reaction containing a certain fraction of a non-canonical nucleotide may yield RNA containing a different, often lower, fraction of the non-canonical nucleotide than the fraction at which the non-canonical nucleotide was present in the reaction. In certain embodiments, references to nucleotide incorporation fractions (for example, “50% 5-methyluridine”) therefore can refer both to nucleic acids containing the stated fraction of the nucleotide, and to nucleic acids synthesized in a reaction containing the stated fraction of the nucleotide (or nucleotide derivative, for example, nucleotide-triphosphate), even though such a reaction may yield a nucleic acid containing a different fraction of the nucleotide than the fraction at which the non-canonical nucleotide was present in the reaction. In addition, different nucleotide sequences can encode the same protein by utilizing alternative codons. In certain embodiments, references to nucleotide incorporation fractions therefore can refer both to nucleic acids containing the stated fraction of the nucleotide, and to nucleic acids encoding the same protein as a different nucleic acid, wherein the different nucleic acid contains the stated fraction of the nucleotide.

Certain embodiments are directed to a nucleic acid comprising a 5′-cap structure selected from Cap 0, Cap 1, Cap 2, and Cap 3 or a derivative thereof. In one embodiment, the nucleic acid comprises one or more UTRs. In another embodiment, the one or more UTRs increase the stability of the nucleic acid. In a further embodiment, the one or more UTRs comprise an alpha-globin or beta-globin 5′-UTR. In a still further embodiment, the one or more UTRs comprise an alpha-globin or beta-globin 3′-UTR. In a still further embodiment, the synthetic RNA molecule comprises an alpha-globin or beta-globin 5′-UTR and an alpha-globin or beta-globin 3′-UTR. In one embodiment, the 5′-UTR comprises a Kozak sequence that is substantially similar to the Kozak consensus sequence. In another embodiment, the nucleic acid comprises a 3′-poly(A) tail. In a further embodiment, the 3′-poly(A) tail is between about 20 nt and about 250 nt or between about 120 nt and about 150 nt long. In a further embodiment, the 3′-poly(A) tail is about 20 nt, or about 30 nt, or about 40 nt, or about 50 nt, or about 60 nt, or about 70 nt, or about 80 nt, or about 90 nt, or about 100 nt, or about 110 nt, or about 120 nt, or about 130 nt, or about 140 nt, or about 150 nt, or about 160 nt, or about 170 nt, or about 180 nt, or about 190 nt, or about 200 nt, or about 210 nt, or about 220 nt, or about 230 nt, or about 240 nt, or about 250 nt long.

Certain embodiments are directed to methods of making nucleic acid drugs, including RNA comprising one or more non-canonical nucleotides. Such methods yield substantially stable RNA.

In various embodiments, the present methods and compositions find use in methods of treating, preventing or ameliorating a disease, disorder and/or condition. For instance, in some embodiments, the described methods of in vivo delivery, including various effective doses, administration strategies and formulations are used in a method of treatment.

In various embodiments, the present methods and compositions find use in methods of altering, modifying and/or changing a tissue (e.g. cosmetically).

In various embodiments, the present methods and compositions include using a nucleic acid drug, including a synthetic RNA, in the diagnosing, treating, preventing or ameliorating of a disease, disorder and/or condition described herein. In various embodiments, the present methods and compositions include using a nucleic acid drug, including a synthetic RNA, in the altering, modifying and/or changing of a tissue (e.g. cosmetically).

Generally speaking, in various embodiments, a synthetic RNA as described herein is administered to a human at specific doses described herein and the synthetic RNA comprises a sequence, sometimes referred to as a target sequence that encodes a protein of interest, which may be a therapeutic protein.

Synthetic RNA comprising only canonical nucleotides can bind to pattern recognition receptors, can be recognized as a pathogen-associated molecular pattern, and can trigger a potent immune response in cells, which can result in translation block, the secretion of inflammatory cytokines, and cell death. It has now been discovered that synthetic RNA comprising certain non-canonical nucleotides can evade detection by the innate immune system, and can be translated at high efficiency into protein, including in humans. It has been further discovered that synthetic RNA comprising at least one of the non-canonical nucleotides described herein, including, for example, a member of the group: 5-methylcytidine, 5-hydroxycytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, pseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-methoxyuridine, 5-formyluridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-methoxypseudouridine, and 5-formylpseudouridine can evade detection by the innate immune system, and can be translated at high efficiency into protein, including in humans. Certain embodiments are therefore directed to a method for inducing a cell to express a protein of interest comprising contacting a cell with synthetic RNA. Other embodiments are directed to a method for transfecting a cell with synthetic RNA comprising contacting a cell with a solution comprising one or more synthetic RNA molecules. Still other embodiments are directed to a method for treating a patient comprising administering to the patient synthetic RNA. In one embodiment, the synthetic RNA comprises at least one of the non-canonical nucleotides described herein, including, for example, a member of the group: 5-methylcytidine, 5-hydroxycytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-methoxycytidine, pseudouridine, 5-hydroxyuridine, 5-methyluridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-methoxyuridine, 5-formyluridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-hydroxymethylpseudouridine, 5-carboxypseudouridine, 5-methoxypseudouridine, and 5-formylpseudouridine. In another embodiment, the synthetic RNA encodes a protein of interest. Exemplary RNAs may contain combinations and levels of non-canonical and non-canonical nucleotides as described elsewhere herein, including with respect to the expression of any protein of interest described herein. In yet another embodiment, the method results in the expression of the protein of interest. In a further embodiment, the method results in the expression of the protein of interest in the patient's skin.

Other embodiments are directed to a method for delivering a nucleic acid to a cell in vivo. Still other embodiments are directed to a method for inducing a cell in vivo to express a protein of interest. Still other embodiments are directed to a method for treating a patient. In one embodiment, the method comprises disrupting the stratum corneum. In another embodiment, the method comprises contacting a cell with a nucleic acid. In yet another embodiment, the method results in the cell internalizing the nucleic acid. In a further embodiment, the method results in the cell expressing the protein of interest. In a still further embodiment, the method results in the expression of the protein of interest in the patient. In a still further embodiment, the method results in the amelioration of one or more of the patient's symptoms. In a still further embodiment, the patient is in need of the protein of interest. In a still further embodiment, the patient is deficient in the protein of interest.

Still other embodiments are directed to a method for treating a patient comprising delivering to a patient a composition. In one embodiment, the composition comprises albumin that is treated with an ion-exchange resin or charcoal. In another embodiment, the composition comprises one or more nucleic acid molecules. In yet another embodiment, at least one of the one or more nucleic acid molecules encodes a protein of interest. In one embodiment, the method results in the expression of the protein in the patient's skin. In another embodiment, the method results in the expression of a therapeutically or cosmetically effective amount of the protein of interest in the patient. In yet another embodiment, the method comprises administering a steroid. In a further embodiment, the steroid is a member of the group: hydrocortisone and dexamethasone.

Some embodiments are directed to a therapeutic composition and/or methods of treatment comprising a nucleic acid molecule encoding one or more proteins, wherein at least one of the one or more proteins is an extracellular matrix protein. Still other embodiments are directed to a cosmetic composition comprising a nucleic acid molecule encoding one or more proteins, wherein at least one of the one or more proteins is an extracellular matrix protein.

Pigmentation disorders can cause severe symptoms in patients. It has now been discovered that pigmentation disorders can be treated by delivering to a patient a nucleic acid encoding tyrosinase. Certain embodiments are therefore directed to a method for treating a pigmentation disorder. Other embodiments are directed to a method for altering the pigmentation of a patient. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding tyrosinase. Other embodiments are directed to a cosmetic composition comprising a nucleic acid encoding tyrosinase. Still other embodiments are directed to a therapeutic composition comprising a nucleic acid encoding tyrosinase. Still other embodiments are directed to a method for increasing the ultraviolet absorption of a patient's skin. In one embodiment the method comprises delivering to a patient a nucleic acid encoding tyrosinase. In another embodiment, the method results in an increase in the ultraviolet absorption of the patient's skin. Still other embodiments are directed to a method for reducing photodamage to a person's skin upon exposure to ultraviolet light. In one embodiment, the method results in the reduction of photodamage to the person's skin upon exposure to ultraviolet light. Still other embodiments are directed to a method for treating xeroderma pigmentosum. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding tyrosinase. Still other embodiments are directed to a method for treating epidermolysis bullosa. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding one or more of keratin 5, keratin 14, plectin, an integrin family member, laminin, a laminin subunit, collagen XVII, collagen VII or a biologically active fragment, variant, analogue or family-member thereof. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding collagen type VII. In another embodiment, the method comprises delivering to a patient a nucleic acid encoding melanocortin 1 receptor. Still other embodiments are directed to a method for treating xerosis. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding a hyaluronan synthase. In another embodiment, the patient is diagnosed with atopic dermatitis. In yet another embodiment, the patient is diagnosed with ichthyosis. Certain embodiments are directed to a method for treating a cosmetic condition. Other embodiments are directed to a method for inducing tissue healing. In one embodiment, the method comprises delivering to a patient a nucleic acid encoding a hyaluronan synthase. In another embodiment, the cosmetic condition is a member of the group: wrinkles, sagging skin, thin skin, discoloration, and dry skin. In yet another embodiment, the patient has had cataract surgery. In some embodiments, the nucleic acid is synthetic RNA. In other embodiments, the method results in the amelioration of one or more of the patient's symptoms. Other embodiments are directed to a method for treating an indication by delivering to a cell or a patient a nucleic acid encoding a protein or a peptide. Still other embodiments are directed to a composition comprising a nucleic acid encoding a protein or a peptide. Indications that can be treated using the methods and compositions of the present invention and proteins and peptides that can be encoded by compositions of the present invention are set forth in Table 2A and/or Table 2B, and are given by way of example, and not by way of limitation. In one embodiment, the indication is selected from Table 2A and/or Table 2B. In another embodiment the protein or peptide is selected from Table 2A and/or Table 2B. In yet another embodiment, the indication and the protein or peptide are selected from the same row of Table 2A and/or Table 2B. In a further embodiment, the protein of interest is a member of the group: UCP1, UCP2, and UCP3. Other embodiments are directed to methods for inducing a cell to express a plurality of proteins of interest. In one embodiment, the proteins of interest include at least two members of the group: a lipase, UCP1, UCP2, and UCP3. In another embodiment, the proteins of interest include a lipase and a member of the group: UCP1, UCP2, and UCP3. In another embodiment, the protein is a gene-editing protein. In yet another embodiment, the gene-editing protein targets a gene that is at least partly responsible for a disease phenotype. In yet another embodiment, the gene-editing protein targets a gene that encodes a protein selected from Table 2A and/or Table 2B. In still another embodiment, the gene-editing protein corrects or eliminates, either alone or in combination with one or more other molecules or gene-editing proteins, a mutation that is at least partly responsible for a disease phenotype.

In various embodiments, the present invention contemplates the targeting of the precursor forms and/or mature forms and/or isoforms and/or mutants of any of the proteins disclosed in Table 2A and/or Table 2B and such proteins. In some embodiments, any of the precursor forms and/or mature forms and/or isoforms and/or mutants have enhanced secretion relative to the corresponding wild type proteins. In some embodiments, any of the precursor forms and/or mature forms and/or isoforms and/or mutants have altered half-lives (e.g. serum, plasma, intracellular)—for instance, longer or shorter half-lives. In some embodiments, this is relative to wild type.

TABLE 2A

Illustrative Indications

Illustrative Indication
Illustrative Protein/Peptide

Acne
Retinol Dehydrogenase 10

Aging
Elastin

sp|P15502|ELN_HUMAN Elastin

(isoform 3)

(SEQ ID NO: 486)

Aging
Collagen Type I

P02452|CO1A1_HUMAN Collagen alpha-1(I) chain

(SEQ ID NO: 487)

P08123|CO1A2_HUMAN Collagen alpha-2(I) chain

(SEQ ID NO: 488)

Aging
Collagen Type III

P02461|CO3A1_HUMAN Collagen alpha-1(III) chain

(isoform 1)

(SEQ ID NO: 489)

Aging
Collagen Type VII

Q02388|CO7A1_HUMAN Collagen alpha-1(VII) chain

(SEQ ID NO: 490)

Aging
Hyaluronan Synthase

Aging
Telomerase Reverse Transcriptase

Albinism
Tyrosinase

P14679|TYRO_HUMAN Tyrosinase

(isoform 1)

(SEQ ID NO: 491)

Alport Syndrome
Collagen Type IV

P02462|CO4A1_HUMAN Collagen alpha-1(IV) chain

(isoform 1)

(SEQ ID NO: 492)

P08572|CO4A2_HUMAN Collagen alpha-2(IV) chain

(SEQ ID NO: 493)

Q01955|CO4A3_HUMAN Collagen alpha-3(IV) chain

(isoform 1)

(SEQ ID NO: 494)

P53420|CO4A4_HUMAN Collagen alpha-4(IV) chain

(SEQ ID NO: 495)

P29400|CO4A5_HUMAN Collagen alpha-5(IV) chain

(isoform 1)

(SEQ ID NO: 496)

Q14031|CO4A6_HUMAN Collagen alpha-6(IV)

(isoform A)

(SEQ ID NO: 497)

Anemia
Erythropoietin

Atopic Dermatitis
Filaggrin

Cutis Laxa
Elastin

sp|P15502|ELN_HUMAN Elastin

(isoform 3)

(SEQ ID NO: 486)

Dry Skin
Filaggrin

Dystrophic Epidermolysis Bullosa
Collagen Type VII

Q02388|CO7A1_HUMAN Collagen alpha-1(VII) chain

(SEQ ID NO: 498)

Ehlers-Danlos Syndrome
Collagen Type V

P20908|CO5A1_HUMAN Collagen alpha-1(V) chain

(SEQ ID NO: 499)

P05997|CO5A2_HUMAN Collagen alpha-2(V) chain

(SEQ ID NO: 500)

P25940|CO5A3_HUMAN Collagen alpha-3(V) chain

(SEQ ID NO: 501)

Ehlers-Danlos Syndrome
Collagen Type I

P02452|CO1A1_HUMAN Collagen alpha-1(I) chain

(SEQ ID NO: 487)

P08123|CO1A2_HUMAN Collagen alpha-2(I) chain

(SEQ ID NO: 488)

Epidermolysis bullosa, lethal acantholytic
ADAM17

P78536|ADA17_HUMAN Disintegrin and

metalloproteinase domain-containing protein 17

(isoform A)

(SEQ ID NO: 502)

Epidermolysis bullosa, type IV
Collagen Type III

P02461|CO3A1_HUMAN Collagen alpha-1(III) chain

(isoform 1)

(SEQ ID NO: 489)

Erythropoietic Protoporphyria
Ferrochelatase

P22830|HEMH_HUMAN Ferrochelatase,

mitochondrial

(isoform 1)

(SEQ ID NO: 503)

Eczema
Filaggrin

Excess Fat
Thermogenin

P25874|UCP1_HUMAN Mitochondrial brown fat

uncoupling protein 1

(SEQ ID NO: 504)

Excess Fat
Lipase

Lipoprotein lipase

P06858|LIPL_HUMAN Lipoprotein lipase

(SEQ ID NO: 516)

Hepatic lipase

P11150|LIPC_HUMAN Hepatic triacylglycerol lipase

(SEQ ID NO: 517)

Pancreatic lipase

P16233|LIPP_HUMAN Pancreatic triacylglycerol

lipase

(SEQ ID NO: 518)

Endothelial lipase

(isoform 1)

Q9Y5X9|LIPE_HUMAN Endothelial lipase

(SEQ ID NO: 519)

Lysosomal lipase

P38571|LICH_HUMAN Lysosomal acid

lipase/cholesteryl ester hydrolase

(isoform 1)

(SEQ ID NO: 520)

Hormone sensitive lipase

Q05469|LIPS_HUMAN Hormone-sensitive lipas

(isoform 1)

(SEQ ID NO: 521)

Gastric lipase

P07098|LIPG_HUMAN Gastric triacylglycerol lipase

(isoform 1)

(SEQ ID NO: 522)

Pancreatic Lipase-Related Protein 1)

P54315|LIPR1_HUMAN Inactive pancreatic lipase-

related protein 1

(isoform 1)

(SEQ ID NO: 523)

Pancreatic Lipase-Related Protein 2

P54317|LIPR2_HUMAN Pancreatic lipase-related

protein 2

(SEQ ID NO: 524)

Carboxyl Ester Lipase

P19835|CEL_HUMAN Bile salt-activated lipase

(isoform long)

(SEQ ID NO: 525)

Hypotrichosis
ADAM17

P78536|ADA17_HUMAN Disintegrin and

metalloproteinase domain-containing protein 17

(isoform A)

(SEQ ID NO: 502)

Ichthyosis Vulgaris
Filaggrin

Infections
Genetic Antibiotics (e.g. Anti-Sigma Factors)

Inflammatory and Bullous Skin Bowel Syndrome
Desmoglein 2

Q14126|DSG2_HUMAN Desmoglein-2

(SEQ ID NO: 505)

Keratosis Pilaris
Retinol Dehydrogenase 10

Oily Skin
Retinol Dehydrogenase 10

Osteoarthritis
Hyaluronan Synthase

Pemphigus Vulgaris
Plakophilin-1

Q13835|PKP1_HUMAN Plakophilin-1

(isoform 2)

(SEQ ID NO: 506)

Pseudoxanthoma elasticum
Elastin

sp|P15502|ELN_HUMAN Elastin

(isoform 3)

(SEQ ID NO: 486)

Psoriasis
Retinol Dehydrogenase 10

Scar Treatment
Tyrosinase

P14679|TYRO_HUMAN Tyrosinase

(isoform 1)

(SEQ ID NO: 491)

Scarring
Elastin

sp|P15502|ELN_HUMAN Elastin

(isoform 3)

(SEQ ID NO: 486)

Scarring
Collagen Type I

P02452|CO1A1_HUMAN Collagen alpha-1(I) chain

(SEQ ID NO: 487)

P08123|CO1A2_HUMAN Collagen alpha-2(I) chain

(SEQ ID NO: 488)

Scarring
Collagen Type III

P02461|CO3A1_HUMAN Collagen alpha-1(III) chain

(isoform 1)

(SEQ ID NO: 489)

Skin Cancer
Interferon

Interferon, Alpha 1

P01562|IFNA1_HUMAN Interferon alpha-1/13

(SEQ ID NO: 530)

Interferon, Alpha 2

P01563|IFNA2_HUMAN Interferon alpha-2

(SEQ ID NO: 531)

Interferon, Alpha 4

P05014|IFNA4_HUMAN Interferon alpha-4

(SEQ ID NO: 532)

Interferon, Alpha 5

P01569|IFNA5_HUMAN Interferon alpha-5

(SEQ ID NO: 533)

Interferon, Alpha 6

P05013|IFNA6_HUMAN Interferon alpha-6

(SEQ ID NO: 534)

Interferon, Alpha 7

P01567|IFNA7_HUMAN Interferon alpha-7

(SEQ ID NO: 535)

Interferon, Alpha 8

P32881|IFNA8_HUMAN Interferon alpha-8

(SEQ ID NO: 536)

Interferon, Alpha 10

P01566|IFN10_HUMAN Interferon alpha-10

(SEQ ID NO: 537)

Interferon, Alpha 14

P01570|IFN14_HUMAN Interferon alpha-14 OS

(SEQ ID NO: 538)

Interferon, Alpha 16

P05015|IFN16_HUMAN Interferon alpha-16

(SEQ ID NO: 539)

Interferon, Alpha 17

P01571|IFN17_HUMAN Interferon alpha-17

(SEQ ID NO: 540)

Interferon, Alpha 21

P01568|IFN21_HUMAN Interferon alpha-21

(SEQ ID NO: 541)

Interferon, Gamma

P01579|IFNG_HUMAN Interferon gamma

(SEQ ID NO: 542)

Interferon, Beta

P01574|IFNB_HUMAN Interferon beta

(SEQ ID NO: 543)

Interferon, Kappa

Q9P0W0|IFNK_HUMAN Interferon kappa

(SEQ ID NO: 544)

Interferon, Epsilon

Q86WN2|IFNE_HUMAN Interferon epsilon

(SEQ ID NO: 545)

Striate Palmoplantar Keratoderma
ADAM17

P78536|ADA17_HUMAN Disintegrin and

metalloproteinase domain-containing protein 17

(isoform A)

(SEQ ID NO: 502)

Tanning
Tyrosinase

P14679|TYRO_HUMAN Tyrosinase

(isoform 1)

(SEQ ID NO: 491)

Vitiligo
Melanocyte-Stimulating Hormone

Alpha-MSH

P01189|138-150

(SEQ ID NO: 526)

Beta-MSH

P01189|217-234

(SEQ ID NO: 527)

Gamma-MSH

P01189|77-87

(SEQ ID NO: 528)

Proopiomelanocortin

P01189|COLI_HUMAN Pro-opiomelanocortin

(SEQ ID NO: 529)

Vitiligo
Tyrosinase

P14679|TYRO_HUMAN Tyrosinase

(isoform 1)

(SEQ ID NO: 491)

Warts
Interferon

Interferon, Alpha 1

P01562|IFNA1_HUMAN Interferon alpha-1/13

(SEQ ID NO: 530)

Interferon, Alpha 2

P01563|IFNA2_HUMAN Interferon alpha-2

(SEQ ID NO: 531)

Interferon, Alpha 4

P05014|IFNA4_HUMAN Interferon alpha-4

(SEQ ID NO: 532)

Interferon, Alpha 5

P01569|IFNA5_HUMAN Interferon alpha-5

(SEQ ID NO: 533)

Interferon, Alpha 6

P05013|IFNA6_HUMAN Interferon alpha-6

(SEQ ID NO: 534)

Interferon, Alpha 7

P01567|IFNA7_HUMAN Interferon alpha-7

(SEQ ID NO: 535)

Interferon, Alpha 8

P32881|IFNA8_HUMAN Interferon alpha-8

(SEQ ID NO: 536)

Interferon, Alpha 10

P01566|IFN10_HUMAN Interferon alpha-10

(SEQ ID NO: 537)

Interferon, Alpha 14

P01570|IFN14_HUMAN Interferon alpha-14 OS

(SEQ ID NO: 538)

Interferon, Alpha 16

P05015|IFN16_HUMAN Interferon alpha-16

(SEQ ID NO: 539)

Interferon, Alpha 17

P01571|IFN17_HUMAN Interferon alpha-17

(SEQ ID NO: 540)

Interferon, Alpha 21

P01568|IFN21_HUMAN Interferon alpha-21

(SEQ ID NO: 541)

Interferon, Gamma

P01579|IFNG_HUMAN Interferon gamma

(SEQ ID NO: 542)

Interferon, Beta

P01574|IFNB_HUMAN Interferon beta

(SEQ ID NO: 543)

Interferon, Kappa

Q9P0W0|IFNK_HUMAN Interferon kappa

(SEQ ID NO: 544)

Interferon, Epsilon

Q86WN2|IFNE_HUMAN Interferon epsilon

(SEQ ID NO: 545)

Wound Healing
Elastin

sp|P15502|ELN_HUMAN Elastin

(isoform 3)

(SEQ ID NO: 486)

Wound Healing
Collagen Type I

P02452|CO1A1_HUMAN Collagen alpha-1(I) chain

(SEQ ID NO: 487)

P08123|CO1A2_HUMAN Collagen alpha-2(I) chain

(SEQ ID NO: 488)

Wound Healing
Collagen Type III

P02461|CO3A1_HUMAN Collagen alpha-1(III) chain

(isoform 1)

(SEQ ID NO: 489)

Xeroderma Pigmentosum
DNA Polymerase Eta

Q9Y253|POLH_HUMAN DNA polymerase eta

(isoform 1)

(SEQ ID NO: 507)

Additional illustrative targets of the present invention include the cosmetic targets listed in Table 6 of International Patent Publication No. WO 2013/151671, the contents of which are hereby incorporated by reference in their entirety.

In various embodiments, the agents of the present invention are used in methods the effect the integumentary system of a human. The present compositions and methods may be used to alter a biological and/or physiological process to, for example, reduce skin sagging, increase skin thickness, increase skin volume, reduce the number of wrinkles, the length of wrinkles and/or the depth of wrinkles, increase skin tightness, firmness, tone and/or elasticity, increase skin hydration and ability to retain moisture, water flow and osmotic balance, increase the levels of skin lipids; increase the extracellular matrix and/or adhesion and communication polypeptides; increase skin energy production; utilization and conservation; improve oxygen utilization; improve skin cell life; improve skin cell immunity defense, heat shock stress response, antioxidant defense capacity to neutralize free radicals, and/or toxic defense; improve the protection and recovery from ultraviolet rays; improve skin cell communication and skin cell innervations; improve cell cohesion/adhesion; improve calcium mineral and other mineral metabolism; improve cell turnover; and improve cell circadian rhythms.

Further still, in some embodiments, the present compositions may be used in the treatment, control, or prevention of a disease, disorder and/or condition and/or may alter, modify or change the appearance of a member of the integumentary system of a subject suffering from a disease, disorder and/or condition such as, but not limited to, acne vulgaris, acne aestivalis, acne conglobata, acne cosmetic, acne fulminans, acne keloidalis nuchae, acne mechanica, acne medicamentosa, acne miliaris necrotica, acne necrotica, acne rosacea, actinic keratosis, acne vulgaris, acne aestivalis, acne conglobata, acne cosmetic, acne fulminans, acne keloidalis nuchae, acne mechanica, acne medicamentosa, acne miliaris necrotica, acne necrotica, acne rosacea, acute urticaria, allergic contact dermatitis, alopecia areata, angioedema, athlete's foot, atopic dermatitis, autoeczematization, baby acne, balding, bastomycosis, blackheads, birthmarks and other skin pigmentation problems, boils, bruises, bug bites and stings, burns, cellulitis, chiggers, chloracne, cholinergic or stress uricara, chronic urticara, cold type urticara, confluent and reticulated papillomatosis, corns, cysts, dandruff, dermatitis herpetiformis, dermatographism, dyshidrotic eczema, diaper rash, dry skin, dyshidrosis, ectodermal dysplasia such as, hyprohidrotic ectodermal dysplasia and X-linked hyprohidrotic ectodermal dysplasia, eczema, epidermaodysplasia verruciformis, erythema nodosum, excoriated acne, exercise-induced anaphylasis folliculitis, excess skin oil, folliculitis, freckles, frostbite, fungal nails, hair density, hair growth rate, halogen acne, hair loss, heat rash, hematoma, herpes simplex infections (e.g. non-genital), hidradenitis suppurativa, hives, hyperhidrosis, hyperpigmentation, hypohidrotic ectodermal dysplasia, hypopigmentation, impetigo, ingrown hair, heat type urticara, ingrown toenail, infantile acne or neonatal acne, itch, irritant contact dermatitis, jock itch, keloid, keratosis pilaris, lichen planus, lichen sclerosus, lupus miliaris disseminatus faciei, melasma, moles, molluscum contagiosum, nail growth rate, nail health, neurodermatitis, nummular eczema, occupational acne, oil acne, onychomycosis, physical urticara, pilonidal cyst, pityriasis rosea, pityriasis versicolor, poison ivy, pomade acne, pseudofolliculitis barbae or acne keloidalis nuchae, psoriasis, psoriatic arthritis, pressure or delayed pressue urticara, puncture wounds such as cuts and scrapes, rash, rare or water type urticara, rhinoplasty, ringworm, rosacea, rothmund-thomson syndrome, sagging of the skin, scabis, scars, seborrhea, seborrheic dermatitis, shingles, skin cancer, skin tag, solar type urticara, spider bite, stretch marks, sunburn, tar acne, tropical acne, thinning of skin, thrush, tinea versicolor, transient acantholytic dermatosis, tycoon's cap or acne necrotica miliaris, uneven skin tone, varicose veins, venous eczema, vibratory angioedema, vitiligo, warts, Weber-Christian disease, wrinkles, x-linked hypohidrotic ectodermal dysplasia, xerotic eczema, yeast infection and general signs of aging.

In some embodiments, there is provided methods of treating, controlling or preventing dry skin with the present compositions. In some embodiments profilaggrin (a protein which is converted to filaggrin) is a protein of interest (e.g. when treating ichthyosis vulgaris).

In some embodiments, there is provided methods of treating, controlling or preventing any one of the various types of psoriasis (e.g. plague psoriasis, guttate psoriasis, pustular psoriasis, inverse psoriasis, and erythrodermic psoriasis). In various embodiments, the protein of interest is any of the products of the genes psoriasis susceptibility 1 through 9 (PSORSI-PSORS9).

Various embodiments relate to the treatment, control, or prevention of eczema (e.g. atopic dermatitis, nummular eczema, dyshidrotic eczema, seborrheic dermatitis, irritant contact dermatitis, allergic contact dermatitis, dyshidrosis, venous eczema, dermatitis herpetiformis, neurodermatitis, autoeczematization and xerotic eczema) and, optionally, one or more of the following may be targeted: filaggrin; three genetic variants, ovo-like 1 (OVOL1), actin-like 9 (ACTL9) and kinesin family member 3 A (KIF3A) have been associated with eczema; and the genes brain-derived neurotrophic factor (BDNF) and tachykinin, precursor 1 (TAC1).

Hives, or urticaria, including, but not limited to, acute urticaria, chronic urticara and angioedema, physical urticara, pressure or delayed pressue urticara, cholinergic or stress uricara, cold type urticara, heat type urticara, solar type urticara, rare or water type urticara, vibratory angioedema, exercise-induced anaphylasis and dermatographism may be treated with the present compositions by, for example, targeting PLCG-2.

Various embodiments relate to the treatment, control, or prevention of rosacea, which includes, but is not limited to, erthematotelangiectatic rosacea, papulopustular rosacea, phymatous rosacea, and ocular rosacea. Optionally, cathelicidin antimicrobial peptide (CAMP) and/or kallikrein-related peptidase 5 (also known as stratum corneum tryptic enzyme (SCTE)) are proteins of interest.

In some embodiments, there is provided methods of treating, controlling or preventing acne with the present compositions. For example, acne may include, but is not limited to, acneiform eruptions, acne aestivalis, acne conglobata, acne cosmetic, acne fulminans, acne keloidalis nuchae, acne mechanica, acne medicamentosa, acne miliaris necrotica, acne necrotica, acne rosacea, baby acne, blackheads, chloracne, excoriated acne, halogen acne, infantile acne or neonatal acne, lupus miliaris disseminatus faciei, occupational acne, oil acne, pomade acne, tar acne, tropical acne, tycoon's cap or acne necrotica miliaris, pseudofolliculitis barbae or acne keloidalis nuchae, and hidradenitis suppurativa. In these embodiments, the protein of interest may be one or more matrix metalloproteinases (MMP), e.g., matrix metalloproteinase-1 (MMP-1 or interstitial collagenase), matrix metalloproteinase-9 (MMP-9), and matrix metalloproteinase-13 (MMP-13).

In further embodiments, vitiligo is treated with the present compositions, e.g. wherein the NLR family, pyrin domain containing 1 gene (NALP1) gene is targeted.

In some embodiments, the present compositions find use in the treatment, control, or prevention of hyprohidrotic ectodermal dysplasia (HED), e.g. via the ectodysplasin A gene (EDA), receptor (EDAR), and receptor associated death domain (EDARADD).

In some embodiments, the present compositions find use in the treatment, control, or prevention of balding, or hair thinning (e.g. male pattern baldness, or androgenetic alopecia (AGA)) and, optionally, one or more of the following may be the protein of interest: androgen receptor (AR), ectodysplasin A2 receptor (EDA2R) and lysophosphatidic acid receptor 6 (P2RY5).

The present compositions also find use in methods of treatment, control, or prevention of scars and stretch marks (striae), e.g. via collagen, ribosomal s6 kinase, sectrected phosphoprotein 1 (also known as osteopontin), or transforming growth factor beta 3.

Epidermodysplasia verruciformis (also known as Lutz-Lewandowsky epidermodysplasia), a rare autosomal recessive genetic hereditary skin disorder, may also be treated with compositions of the present invention, e.g. by targeted transmembrane channel-like 6 (EVER1) or transmembrane channellike 8 (EVER2) genes.

In some embodiments, skin sagging, thinning or wrinkling may be treated, controlled or prevented with present composition, e.g. by targeting one or more of the proteins of interest such as collagen, elastin, fibroblast growth factor 7, TIMP metallopeptidase inhibitors, matrix metallopeptidases, superoxide dismutase and other extracellular matrix proteins and proteoglycans.

Further embodiments are used in tanning of the skin, such as via melanocyte-stimulating hormone and/or pro-opiomelanocortin.

In some embodiments, the present compositions may be used for wound treatment. In some embodiments, methods of treating, controlling or preventing wounds with the present compositions comprises additional steps of, for example, cleaning the wound bed to facilitate wound healing and closure, including, but not limited to: debridement, sharp debridement (surgical removal of dead or infected tissue from a wound), optionally including chemical debriding agents, such as enzymes, to remove necrotic tissue; wound dressings to provide the wound with a moist, warm environment and to promote tissue repair and healing (e.g., wound dressings comprising hydrogels (e.g., AQUASORB; DUODERM), hydrocolloids (e.g., AQUACEL; COMFEEL), foams (e.g., LYOFOAM; SPYROSORB), and alginates (e.g., ALGISITE; CURASORB); administration of growth factors to stimulate cell division and proliferation and to promote wound healing e.g. becaplermin; and (iv) soft-tissue wound coverage, a skin graft may be necessary to obtain coverage of clean, non-healing wounds (e.g., autologous skin grafts, cadaveric skin graft, bioengineered skin substitutes (e.g., APLIGRAF; DERMAGRAFT)).

In various embodiments, the nucleic acid drug described herein can be used in a variety of cosmetic/plastic surgery procedures, including, without limitation, a surgical procedure involving skin grafting and an aesthetic or cosmetic surgery (e.g. a facial plastic surgery procedure including, but not limited to blepharoplasty, rhinoplasty, rhytidectomy, genioplasty, facial implants, otoplasty, hair implantation, cleft lip and cleft palate repair, and/or a body plastic surgery procedure including but not limited to abdominoplasty, brachioplasty, thigh lift, breast reduction, breast augmentation, body contouring, liposuction, hand surgery).

In various embodiments, a variety of cancers are treated, controlled or prevented with the present compositions (e.g., colorectal cancer, gallbladder cancer, lung cancer, pancreatic cancer, and stomach cancer). In some embodiments, skin cancer is treated with the present compositions. For instance, the skin cancer is one or more of actinic keratosis, basal cell carcinoma, melanoma, Kaposi's sarcoma, and squamous cell carcinoma. In some embodiments, the present compositions are used adjuvant to complete circumferential peripheral and deep margin assessment, Mohs surgery, radiation (e.g. external beam radiotherapy or brachytherapy), chemotherapy (including but not limited to topical chemotherapy, e.g. with imiquimod or 5-fluorouracil), and cryotherapy. The present compositions also find use in the treatment of various stages of cancers, including skin cancers (e.g. basal cell cancer (BCC), squamous cell cancer (SCC), and melanoma), such as a stage of the American Joint Committee on Cancer (AJCC) TNM system (e.g. one or more of TX, T0, Tis, T1, T1a, T1b, T2, T2A, T2B, T3, T3a, T3b, T4, T4a, T4b, NX, N0, N1, N2, N3, M0, M1a, M1b, M1c) and/or a staging system (e.g. Stage 0, Stage IA, Stage IB, Stage IIA, Stage IIB, Stage IIC, Stage IIIA, Stage IIIB, Stage IIIC, Stage IV).

Illustrative cancers and/or tumors of the present invention include, but are not limited to, a basal cell carcinoma, biliary tract cancer; bladder cancer; bone cancer; brain and central nervous system cancer; breast cancer; cancer of the peritoneum; cervical cancer; choriocarcinoma; colon and rectum cancer; connective tissue cancer; cancer of the digestive system; endometrial cancer; esophageal cancer; eye cancer; cancer of the head and neck; gastric cancer (including gastrointestinal cancer); glioblastoma; hepatic carcinoma; hepatoma; intra-epithelial neoplasm; kidney or renal cancer; larynx cancer; leukemia; liver cancer; lung cancer (e.g., small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, and squamous carcinoma of the lung); melanoma; myeloma; neuroblastoma; oral cavity cancer (lip, tongue, mouth, and pharynx); ovarian cancer; pancreatic cancer; prostate cancer; retinoblastoma; rhabdomyosarcoma; rectal cancer; cancer of the respiratory system; salivary gland carcinoma; sarcoma; skin cancer; squamous cell cancer; stomach cancer; testicular cancer; thyroid cancer; uterine or endometrial cancer; cancer of the urinary system; vulval cancer; lymphoma including Hodgkin's and non-Hodgkin's lymphoma, as well as B-cell lymphoma (including low grade/follicular non-Hodgkin's lymphoma (NHL); small lymphocytic (SL) NHL; intermediate grade/follicular NHL; intermediate grade diffuse NHL; high grade immunoblastic NHL; high grade lymphoblastic NHL; high grade small non-cleaved cell NHL; bulky disease NHL; mantle cell lymphoma; AIDS-related lymphoma; and Waldenstrom's Macroglobulinemia; chronic lymphocytic leukemia (CLL); acute lymphoblastic leukemia (ALL); Hairy cell leukemia; chronic myeloblastic leukemia; as well as other carcinomas and sarcomas; and post-transplant lymphoproliferative disorder (PTLD), as well as abnormal vascular proliferation associated with phakomatoses, edema (such as that associated with brain tumors), and Meigs' syndrome.

In various embodiments, one or more rare diseases are treated, controlled or prevented with the present compositions, including, by way of illustration, Erythropoietic Protoporphyria, Hailey-Hailey Disease, Epidermolysis Bullosa (EB), Xeroderma Pigmentosum, Ehlers-Danlos Syndrome, Cutis Laxa, Protein C & Protein S Deficiency, Alport Syndrome, Striate Palmoplantar Keratoderma, Lethal Acantholytic EB, Pseudoxanthoma Elasticum (PXE), Ichthyosis Vulgaris, Pemphigus Vulgaris, and Basal Cell Nevus Syndrome.

In various embodiments, the present compositions are used to treat, control or prevent one or more inflammatory diseases or conditions, such as inflammation, acute inflammation, chronic inflammation, respiratory disease, atherosclerosis, restenosis, asthma, allergic rhinitis, atopic dermatitis, septic shock, rheumatoid arthritis, inflammatory bowel disease, inflammatory pelvic disease, pain, ocular inflammatory disease, celiac disease, Leigh Syndrome, Glycerol Kinase Deficiency, Familial eosinophilia (FE), autosomal recessive spastic ataxia, laryngeal inflammatory disease; Tuberculosis, Chronic cholecystitis, Bronchiectasis, Silicosis and other pneumoconioses.

In various embodiments, the present compositions are used to treat, control or prevent one or more autoimmune diseases or conditions, such as multiple sclerosis, diabetes mellitus, lupus, celiac disease, Crohn's disease, ulcerative colitis, Guillain-Barre syndrome, scleroderms, Goodpasture's syndrome, Wegener's granulomatosis, autoimmune epilepsy, Rasmussen's encephalitis, Primary biliary sclerosis, Sclerosing cholangitis, Autoimmune hepatitis, Addison's disease, Hashimoto's thyroiditis, Fibromyalgia, Menier's syndrome; transplantation rejection (e.g., prevention of allograft rejection) pernicious anemia, rheumatoid arthritis, systemic lupus erythematosus, dermatomyositis, Sjogren's syndrome, lupus erythematosus, multiple sclerosis, myasthenia gravis, Reiter's syndrome, Grave's disease, and other autoimmune diseases.

In various embodiments, the present compositions are used to treat, control or prevent one or more neurologic diseases, including ADHD, AIDS—Neurological Complications, Absence of the Septum Pellucidum, Acquired Epileptiform Aphasia, Acute Disseminated Encephalomyelitis, Adrenoleukodystrophy, Agenesis of the Corpus Callosum, Agnosia, Aicardi Syndrome, Alexander Disease, Alpers' Disease, Alternating Hemiplegia, Alzheimer's Disease, Amyotrophic Lateral Sclerosis, Anencephaly, Aneurysm, Angelman Syndrome, Angiomatosis, Anoxia, Aphasia, Apraxia, Arachnoid Cysts, Arachnoiditis, Arnold-Chiari Malformation, Arteriovenous Malformation, Aspartame, Asperger Syndrome, Ataxia Telangiectasia, Ataxia, Attention Deficit-Hyperactivity Disorder, Autism, Autonomic Dysfunction, Back Pain, Barth Syndrome, Batten Disease, Behcet's Disease, Bell's Palsy, Benign Essential Blepharospasm, Benign Focal Amyotrophy, Benign Intracranial Hypertension, Bernhardt-Roth Syndrome, Binswanger's Disease, Blepharospasm, Bloch-Sulzberger Syndrome, Brachial Plexus Birth Injuries, Brachial Plexus Injuries, Bradbury-Eggleston Syndrome, Brain Aneurysm, Brain Injury, Brain and Spinal Tumors, Brown-Sequard Syndrome, Bulbospinal Muscular Atrophy, Canavan Disease, Carpal Tunnel Syndrome, Causalgia, Cavernomas, Cavernous Angioma, Cavernous Malformation, Central Cervical Cord Syndrome, Central Cord Syndrome, Central Pain Syndrome, Cephalic Disorders, Cerebellar Degeneration, Cerebellar Hypoplasia, Cerebral Aneurysm, Cerebral Arteriosclerosis, Cerebral Atrophy, Cerebral Beriberi, Cerebral Gigantism, Cerebral Hypoxia, Cerebral Palsy, Cerebro-Oculo-Facio-Skeletal Syndrome, Charcot-Marie-Tooth Disorder, Chiari Malformation, Chorea, Choreoacanthocytosis, Chronic Inflammatory Demyelinating Polyneuropathy (CIDP), Chronic Orthostatic Intolerance, Chronic Pain, Cockayne Syndrome Type II, Coffin Lowry Syndrome, Coma, including Persistent Vegetative State, Complex Regional Pain Syndrome, Congenital Facial Diplegia, Congenital Myasthenia, Congenital Myopathy, Congenital Vascular Cavernous Malformations, Corticobasal Degeneration, Cranial Arteritis, Craniosynostosis, Creutzfeldt-Jakob Disease, Cumulative Trauma Disorders, Cushing's Syndrome, Cytomegalic Inclusion Body Disease (CIBD), Cytomegalovirus Infection, Dancing Eyes-Dancing Feet Syndrome, Dandy-Walker Syndrome, Dawson Disease, De Morsier's Syndrome, Dejerine-Klumpke Palsy, Dementia—Multi-Infarct, Dementia—Subcortical, Dementia With Lewy Bodies, Dermatomyositis, Developmental Dyspraxia, Devic's Syndrome, Diabetic Neuropathy, Diffuse Sclerosis, Dravet's Syndrome, Dysautonomia, Dysgraphia, Dyslexia, Dysphagia, Dyspraxia, Dystonias, Early Infantile Epileptic Encephalopathy, Empty Sella Syndrome, Encephalitis Lethargica, Encephalitis and Meningitis, Encephaloceles, Encephalopathy, Encephalotrigeminal Angiomatosis, Epilepsy, Erb's Palsy, Erb-Duchenne and Dejerine-Klumpke Palsies, Fabry's Disease, Fahr's Syndrome, Fainting, Familial Dysautonomia, Familial Hemangioma, Familial Idiopathic Basal Ganglia Calcification, Familial Spastic Paralysis, Febrile Seizures (e.g., GEFS and GEFS plus), Fisher Syndrome, Floppy Infant Syndrome, Friedreich's Ataxia, Gaucher's Disease, Gerstmann's Syndrome, Gerstmann-Straussler-Scheinker Disease, Giant Cell Arteritis, Giant Cell Inclusion Disease, Globoid Cell Leukodystrophy, Glossopharyngeal Neuralgia, Guillain-Barre Syndrome, HTLV-1 Associated Myelopathy, Hallervorden-Spatz Disease, Head Injury, Headache, Hemicrania Continua, Hemifacial Spasm, Hemiplegia Alterans, Hereditary Neuropathies, Hereditary Spastic Paraplegia, Heredopathia Atactica Polyneuritiformis, Herpes Zoster Oticus, Herpes Zoster, Hirayama Syndrome, Holoprosencephaly, Huntington's Disease, Hydranencephaly, Hydrocephalus—Normal Pressure, Hydrocephalus, Hydromyelia, Hypercortisolism, Hypersomnia, Hypertonia, Hypotonia, Hypoxia, Immune-Mediated Encephalomyelitis, Inclusion Body Myositis, Incontinentia Pigmenti, Infantile Hypotonia, Infantile Phytanic Acid Storage Disease, Infantile Refsum Disease, Infantile Spasms, Inflammatory Myopathy, Intestinal Lipodystrophy, Intracranial Cysts, Intracranial Hypertension, Isaac's Syndrome, Joubert Syndrome, Kearns-Sayre Syndrome, Kennedy's Disease, Kinsbourne syndrome, Kleine-Levin syndrome, Klippel Feil Syndrome, Klippel-Trenaunay Syndrome (KTS), Kluver-Bucy Syndrome, Korsakoffs Amnesic Syndrome, Krabbe Disease, Kugelberg-Welander Disease, Kuru, Lambert-Eaton Myasthenic Syndrome, Landau-Kleffner Syndrome, Lateral Femoral Cutaneous Nerve Entrapment, Lateral Medullary Syndrome, Learning Disabilities, Leigh's Disease, Lennox-Gastaut Syndrome, Lesch-Nyhan Syndrome, Leukodystrophy, Levine-Critchley Syndrome, Lewy Body Dementia, Lissencephaly, Locked-In Syndrome, Lou Gehrig's Disease, Lupus—Neurological Sequelae, Lyme Disease—Neurological Complications, Machado-Joseph Disease, Macrencephaly, Megalencephaly, Melkersson-Rosenthal Syndrome, Meningitis, Menkes Disease, Meralgia Paresthetica, Metachromatic Leukodystrophy, Microcephaly, Migraine, Miller Fisher Syndrome, Mini-Strokes, Mitochondrial Myopathies, Mobius Syndrome, Monomelic Amyotrophy, Motor Neuron Diseases, Moyamoya Disease, Mucolipidoses, Mucopolysaccharidoses, Multi-Infarct Dementia, Multifocal Motor Neuropathy, Multiple Sclerosis, Multiple System Atrophy with Orthostatic Hypotension, Multiple System Atrophy, Muscular Dystrophy, Myasthenia—Congenital, Myasthenia Gravis, Myelinoclastic Diffuse Sclerosis, Myoclonic Encephalopathy of Infants, Myoclonus, Myopathy—Congenital, Myopathy—Thyrotoxic, Myopathy, Myotonia Congenita, Myotonia, Narcolepsy, Neuroacanthocytosis, Neurodegeneration with Brain Iron Accumulation, Neurofibromatosis, Neuroleptic Malignant Syndrome, Neurological Complications of AIDS, Neurological Manifestations of Pompe Disease, Neuromyelitis Optica, Neuromyotonia, Neuronal Ceroid Lipofuscinosis, Neuronal Migration Disorders, Neuropathy—Hereditary, Neurosarcoidosis, Neurotoxicity, Nevus Cavernosus, Niemann-Pick Disease, O'Sullivan-McLeod Syndrome, Occipital Neuralgia, Occult Spinal Dysraphism Sequence, Ohtahara Syndrome, Olivopontocerebellar Atrophy, Opsoclonus Myoclonus, Orthostatic Hypotension, Overuse Syndrome, Pain—Chronic, Paraneoplastic Syndromes, Paresthesia, Parkinson's Disease, Parnyotonia Congenita, Paroxysmal Choreoathetosis, Paroxysmal Hemicrania, Parry-Romberg, Pelizaeus-Merzbacher Disease, Pena Shokeir II Syndrome, Perineural Cysts, Periodic Paralyses, Peripheral Neuropathy, Periventricular Leukomalacia, Persistent Vegetative State, Pervasive Developmental Disorders, Phytanic Acid Storage Disease, Pick's Disease, Piriformis Syndrome, Pituitary Tumors, Polymyositis, Pompe Disease, Porencephaly, Post-Polio Syndrome, Postherpetic Neuralgia, Postinfectious Encephalomyelitis, Postural Hypotension, Postural Orthostatic Tachycardia Syndrome, Postural Tachycardia Syndrome, Primary Lateral Sclerosis, Prion Diseases, Progressive Hemifacial Atrophy, Progressive Locomotor Ataxia, Progressive Multifocal Leukoencephalopathy, Progressive Sclerosing Poliodystrophy, Progressive Supranuclear Palsy, Pseudotumor Cerebri, Pyridoxine Dependent and Pyridoxine Responsive Seizure Disorders, Ramsay Hunt Syndrome Type I, Ramsay Hunt Syndrome Type II, Rasmussen's Encephalitis and other autoimmune epilepsies, Reflex Sympathetic Dystrophy Syndrome, Refsum Disease-Infantile, Refsum Disease, Repetitive Motion Disorders, Repetitive Stress Injuries, Restless Legs Syndrome, Retrovirus-Associated Myelopathy, Rett Syndrome, Reye's Syndrome, Riley-Day Syndrome, SUNCT Headache, Sacral Nerve Root Cysts, Saint Vitus Dance, Salivary Gland Disease, Sandhoff Disease, Schilder's Disease, Schizencephaly, Seizure Disorders, Septo-Optic Dysplasia, Severe Myoclonic Epilepsy of Infancy (SMEI), Shaken Baby Syndrome, Shingles, Shy-Drager Syndrome, Sjogren's Syndrome, Sleep Apnea, Sleeping Sickness, Soto's Syndrome, Spasticity, Spina Bifida, Spinal Cord Infarction, Spinal Cord Injury, Spinal Cord Tumors, Spinal Muscular Atrophy, Spinocerebellar Atrophy, Steele-Richardson-Olszewski Syndrome, Stiff-Person Syndrome, Striatonigral Degeneration, Stroke, Sturge-Weber Syndrome, Subacute Sclerosing Panencephalitis, Subcortical Arteriosclerotic Encephalopathy, Swallowing Disorders, Sydenham Chorea, Syncope, Syphilitic Spinal Sclerosis, Syringohydromyelia, Syringomyelia, Systemic Lupus Erythematosus, Tabes Dorsalis, Tardive Dyskinesia, Tarlov Cysts, Tay-Sachs Disease, Temporal Arteritis, Tethered Spinal Cord Syndrome, Thomsen Disease, Thoracic Outlet Syndrome, Thyrotoxic Myopathy, Tic Douloureux, Todd's Paralysis, Tourette Syndrome, Transient Ischemic Attack, Transmissible Spongiform Encephalopathies, Transverse Myelitis, Traumatic Brain Injury, Tremor, Trigeminal Neuralgia, Tropical Spastic Paraparesis, Tuberous Sclerosis, Vascular Erectile Tumor, Vasculitis including Temporal Arteritis, Von Economo's Disease, Von Hippel-Lindau disease (VHL), Von Recklinghausen's Disease, Wallenberg's Syndrome, Werdnig-Hoffman Disease, Wernicke-Korsakoff Syndrome, West Syndrome, Whipple's Disease, Williams Syndrome, Wilson's Disease, X-Linked Spinal and Bulbar Muscular Atrophy, and Zellweger Syndrome.

In various embodiments, the present compositions are used to treat one or more respiratory diseases, such as asthma, chronic obstructive pulmonary disease (COPD), bronchiectasis, allergic rhinitis, sinusitis, pulmonary vasoconstriction, inflammation, allergies, impeded respiration, respiratory distress syndrome, cystic fibrosis, pulmonary hypertension, pulmonary vasoconstriction, emphysema, Hantavirus pulmonary syndrome (HPS), Loeffler's syndrome, Goodpasture's syndrome, Pleurisy, pneumonitis, pulmonary edema, pulmonary fibrosis, Sarcoidosis, complications associated with respiratory syncitial virus infection, and other respiratory diseases.

In various embodiments, the present compositions are used to treat, control or prevent cardiovascular disease, such as a disease or condition affecting the heart and vasculature, including but not limited to, coronary heart disease (CHD), cerebrovascular disease (CVD), aortic stenosis, peripheral vascular disease, atherosclerosis, arteriosclerosis, myocardial infarction (heart attack), cerebrovascular diseases (stroke), transient ischaemic attacks (TIA), angina (stable and unstable), atrial fibrillation, arrhythmia, vavular disease, and/or congestive heart failure.

In various embodiments, the present compositions are used to treat, control or prevent one or more metabolic-related disorders. In various embodiments, the present invention is useful for the treatment, controlling or prevention of diabetes, including Type 1 and Type 2 diabetes and diabetes associated with obesity. The compositions and methods of the present invention are useful for the treatment or prevention of diabetes-related disorders, including without limitation diabetic nephropathy, hyperglycemia, impaired glucose tolerance, insulin resistance, obesity, lipid disorders, dyslipidemia, hyperlipidemia, hypertriglyceridemia, hypercholesterolemia, low HDL levels, high LDL levels, atherosclerosis and its sequelae, vascular restenosis, irritable bowel syndrome, inflammatory bowel disease, including Crohn's disease and ulcerative colitis, other inflammatory conditions, pancreatitis, abdominal obesity, neurodegenerative disease, retinopathy, neoplastic conditions, adipose cell tumors, adipose cell carcinomas, such as liposarcoma, prostate cancer and other cancers, including gastric, breast, bladder and colon cancers, angiogenesis, Alzheimer's disease, psoriasis, high blood pressure, Metabolic Syndrome (e.g. a person has three or more of the following disorders: abdominal obesity, hypertriglyceridemia, low HDL cholesterol, high blood pressure, and high fasting plasma glucose), ovarian hyperandrogenism (polycystic ovary syndrome), and other disorders where insulin resistance is a component, such as sleep apnea. The compositions and methods of the present invention are useful for the treatment, control, or prevention of obesity, including genetic or environmental, and obesity-related disorders. The obesity-related disorders herein are associated with, caused by, or result from obesity. Examples of obesity-related disorders include obesity, diabetes, overeating, binge eating, and bulimia, hypertension, elevated plasma insulin concentrations and insulin resistance, dyslipidemia, hyperlipidemia, endometrial, breast, prostate, kidney and colon cancer, osteoarthritis, obstructive sleep apnea, gallstones, heart disease, abnormal heart rhythms and arrythmias, myocardial infarction, congestive heart failure, coronary heart disease, sudden death, stroke, polycystic ovary disease, craniopharyngioma, Prader-Willi Syndrome, Frohlich's syndrome, GH-deficient subjects, normal variant short stature, Turner's syndrome, and other pathological conditions showing reduced metabolic activity or a decrease in resting energy expenditure as a percentage of total fat-free mass, e.g, children with acute lymphoblastic leukemia. Further examples of obesity-related disorders are Metabolic Syndrome, insulin resistance syndrome, reproductive hormone abnormalities, sexual and reproductive dysfunction, such as impaired fertility, infertility, hypogonadism in males and hirsutism in females, fetal defects associated with maternal obesity, gastrointestinal motility disorders, such as obesity-related gastro-esophageal reflux, respiratory disorders, such as obesity-hypoventilation syndrome (Pickwickian syndrome), breathlessness, cardiovascular disorders, inflammation, such as systemic inflammation of the vasculature, arteriosclerosis, hypercholesterolemia, lower back pain, gallbladder disease, hyperuricemia, gout, and kidney cancer, and increased anesthetic risk. The compositions and methods of the present invention are also useful to treat Alzheimer's disease.

Nucleic acids, including liposomal formulations containing nucleic acids, when delivered in vivo, can accumulate in the liver and/or spleen. It has now been discovered that nucleic acids encoding proteins can modulate protein expression in the liver and spleen, and that nucleic acids used in this manner can constitute potent therapeutics for the treatment of liver and spleen diseases. Certain embodiments are therefore directed to a method for treating liver and/or spleen disease by delivering to a patient a nucleic acid encoding a protein of interest. Other embodiments are directed to a therapeutic composition comprising a nucleic acid encoding a protein of interest, for the treatment of liver and/or spleen disease. Diseases and conditions of the liver and/or spleen that can be treated include, but are not limited to: hepatitis, alcohol-induced liver disease, drug-induced liver disease, Epstein Barr virus infection, adenovirus infection, cytomegalovirus infection, toxoplasmosis, Rocky Mountain spotted fever, non-alcoholic fatty liver disease, hemochromatosis, Wilson's Disease, Gilbert's Disease, and cancer of the liver and/or spleen.

In some embodiments, the present compositions and methods relate to the treatment of type 1 diabetes, heart disease, including ischemic and dilated cardiomyopathy, macular degeneration, Parkinson's disease, cystic fibrosis, sickle-cell anemia, thalassemia, Fanconi anemia, severe combined immunodeficiency, hereditary sensory neuropathy, xeroderma pigmentosum, Huntington's disease, muscular dystrophy, amyotrophic lateral sclerosis, Alzheimer's disease, cancer, and infectious diseases including hepatitis and HIV/AIDS.

In various embodiments, the present methods and compositions find use in treating or preventing one or more metabolic diseases or disorders. In various embodiments, the present methods and compositions find use in treating or preventing one or more of diseases or disorders of carbohydrate metabolism. diseases or disorders of amino acid metabolism, diseases or disorders of the urea cycle, diseases or disorders of fatty acid metabolism, diseases or disorders of porphyrin metabolism, lysosomal storage disorders, peroxisome biogenesis disorders, and diseases or disorders of purine or pyrimidine metabolism.

In various embodiments, the present methods and compositions find use in treating or preventing one or more of diseases or disorders in the table below. In various embodiments, the present methods and compositions find use in treating or preventing one or more of diseases or disorders in the table below for instance by modulating the genes associated with the diseases in the table below. In some embodiments, the present methods and compositions find use in gene-editing the genes described in the table below using the present compositions.

Category
Disease
Genes
Entrez ID

Disorders of
Galactosemia
GALT, GALK1, GALE
2592, 2584, 2582

carbohydrate
Essential fructosuria
KHK
3795

metabolism
Hereditary fructose
ALDOB
229

intolerance

Glycogen storage disease
G6PC, SLC37A4, SLC17A3
2538, 2542, 10786

type I

Glycogen storage disease
GAA
2548

type II

Glycogen storage disease
AGL
178

type III

Glycogen storage disease
GBE1
2632

type IV

Glycogen storage disease
PYGM
5837

type V

Glycogen storage disease
PYGL
5836

type VI

Glycogen storage disease
PYGM
5837

type VII

Glycogen storage disease
PHKA1, PHKA2, PHKB,
5255, 5256, 5257, 5260,

type IX
PHKG1, PHKG2
5261

Glycogen storage disease
SLC2A2
6514

type XI

Glycogen storage disease
ALDOA
226

type XII

Glycogen storage disease
ENO1, ENO2, ENO3
2023, 2026, 2027

type XIII

Glycogen storage disease
GYS1, GYS2
2997, 2998

type 0

Pyruvate carboxylase
PC
5091

deficiency

Pyruvate kinase deficiency
PKLR
5313

Transaldolase deficiency
TALDO1
6888

Triosephosphate isomerase
TPI1
7167

deficiency

Fructose bisphosphatase
FBP1
2203

deficiency

Hyperoxaluria
AGXT, GRHPR
189, 9380

Hexokinase deficiency
HK1
3098

Glucose-galactose
SLC5A1
6523

malabsorption

Glucose-6-phosphate
G6PD
2539

dehydrogenase deficiency

Disorders of
Alkaptonuria
HGD
3081

amino acid
Aspartylglucosaminuria
AGA
175

metabolism
Methylmalonic acidemia
MUT, MCEE, MMAA,
4594, 84693, 166785,

MMAB, MMACHC,
326625, 25974, 27249,

MMADHC, LMBRD1
55788

Maple syrup urine disease
BCKDHA, BCKDHB, DBT,
593, 594, 1629, 1738

DLD

Homocystinuria
CBS
875

Tyrosinemia
FAH, TAT, HPD
2184, 6898, 3242

Trimethylaminuria
FMO3
2328

Hartnup disease
SLC6A19
340024

Biotinidase deficiency
BTD
686

Ornithine
OTC
5009

carbamoyltransferase

deficiency

Carbamoyl-phosphate
CPS1
1373

synthase I deficiency

disease

Citrullinemia
ASS, SLC25A13
445, 10165

Hyperargininemia
ARG1
383

Hyperhomocysteinemia
MTHFR
4524

Hypermethioninemia
MAT1A, GNMT, AHCY
4143, 27232, 191

Hyperlysinemias
AASS
10157

Nonketotic hyperglycinemia
GLDC, AMT, GCSH
2731, 275, 2653

Propionic acidemia
PCCA, PCCB
5095, 5096

Hyperprolinemia
ALDH4A1, PRODH
8659, 5625

Cystinuria
SLC3A1, SLC7A9
6519, 11136

Dicarboxylic aminoaciduria
SLC1A1
6505

Glutaric acidemia type 2
ETFA, ETFB, ETFDH
2108, 2109, 2110

Isovaleric acidemia
IVD
3712

2-Hydroxyglutaric aciduria
L2HGDH, D2HGDH
79944, 728294

Disorders of the
N-Acetylglutamate
NAGS
162417

urea cycle
synthase deficiency

Argininosuccinic aciduria
ASL
435

Argininemia
ARG1
383

Disorders of
Very long-chain acyl-
ACADVL
37

fatty acid
coenzyme A

metabolism
dehydrogenase deficiency

Long-chain 3-hydroxyacyl-
HADHA
3030

coenzyme A

dehydrogenase deficiency

Medium-chain acyl-
ACADM
34

coenzyme A

dehydrogenase deficiency

Short-chain acyl-coenzyme
ACADS
35

A dehydrogenase

deficiency

3-hydroxyacyl-coenzyme A
HADH
3033

dehydrogenase deficiency

2,4 Dienoyl-CoA reductase
NADK2
133686

deficiency

3-Hydroxy-3-methylglutaryl-
HMGCL
3155

CoA lyase deficiency

Malonyl-CoA
MLYCD
23417

decarboxylase deficiency

Systemic primary carnitine
SLC22A5
6584

deficiency

Carnitine-acylcarnitine
SLC25A20
788

translocase deficiency

Carnitine
CPT1A
1374

palmitoyltransferase I

deficiency

Carnitine
CPT2
1376

palmitoyltransferase II

deficiency

Lysosomal acid lipase
LIPA
3988

deficiency

Gaucher's disease
GBA
2629

Disorders of
Acute intermittent porphyria
HMBS
3145

porphyrin
Gunther disease
UROS
7390

metabolism
Porphyria cutanea tarda
UROD
7389

Hepatoerythropoietic
UROD
7389

porphyria

Hereditary coproporphyria
CPOX
1371

Variegate porphyria
PPOX
5498

Erythropoietic
FECH
2235

protoporphyria

Aminolevulinic acid
ALAD
210

dehydratase deficiency

porphyria

Lysosomal
Farber disease
ASAH1
427

storage
Krabbe disease
GALC
2581

disorders
Galactosialidosis
CTSA
5476

Fabry disease
GLA
2717

Schindler disease
NAGA
4668

GM1 gangliosidosis
GLB1
2720

Tay-Sachs disease
HEXA
3073

Sandhoff disease
HEXB
3074

GM2-gangliosidosis, AB
GM2A
2760

variant

Niemann-Pick disease
SMPD1, NPC1, NPC2
6609, 4864, 10577

Metachromatic
ARSA, PSAP
410, 5660

leukodystrophy

Multiple sulfatase
SUMF1
285362

deficiency

Hurler syndrome
IDUA
3425

Hunter syndrome
IDS
3423

Sanfilippo syndrome
SGSH, NAGLU, HGSNAT,
6448, 4669, 138050, 2799

GNS

Morquio syndrome
GALNS, GLB1
2588, 2720

Maroteaux-Lamy syndrome
ARSB
411

Sly syndrome
GUSB
2990

Sialidosis
NEU1, NEU2, NEU3, NEU4
4758, 4759, 10825, 129807

I-cell disease
GNPTAB, GNPTG
79158, 84572

Mucolipidosis type IV
MCOLN1
57192

Infantile neuronal ceroid
PPT1, PPT2
5538, 9374

lipofuscinosis

Jansky-Bielschowsky
TPP1
1200

disease

Batten disease
CLN1, CLN2, CLN3, CLN5,
5538, 1200, 1201, 1203,

CLN6, MFSD8, CLN8, CTSD
54982, 256471, 2055, 1509

Kufs disease, Type A
CLN6, PPT1
54982, 5538

Kufs disease, Type B
DNAJC5, CTSF
80331, 8722

Alpha-mannosidosis
MAN2B1, MAN2B2,
4125, 23324, 4123

MAN2C1

Beta-mannosidosis
MANBA
4226

Fucosidosis
FUCA1
2517

Cystinosis
CTNS
1497

Pycnodysostosis
CTSK
1513

Salla disease
SLC17A5
26503

Infantile free sialic acid
SLC17A5
26503

storage disease

Danon disease
LAMP2
3920

Peroxisome
Zellweger syndrome
PEX1, PEX2, PEX3, PEX5,
5189, 5828, 8504, 5830,

biogenesis

PEX6, PEX12, PEX14,
5190, 5193, 5195, 55670

disorders

PEX26

Infantile Refsum disease
PEX1, PEX2, PEX26
5189, 5828, 55670

Neonatal
PEX5, PEX1, PEX10,
5830, 5189, 5192, 5194,

adrenoleukodystrophy
PEX13, PEX26
55670

RCDP Type 1
PEX7
5191

Pipecolic acidemia
PAHX
5264

Acatalasia
CAT
847

Hyperoxaluria type 1
AGXT
189

Acyl-CoA oxidase
ACOX1
51

deficiency

D-bifunctional protein
HSD17B4
3295

deficiency

Dihydroxyacetonephosphate
GNPAT
8443

acyltransferase deficiency

X-linked
ABCD1
215

adrenoleukodystrophy

α-Methylacyl-CoA
AMACR
23600

racemase deficiency

RCDP Type 2
DHAPAT
8443

RCDP Type 3
AGPS
8540

Adult Refsum disease-1
PHYH
5264

Mulibrey nanism
TRIM37
4591

Disorders of
Lesch-Nyhan syndrome
HPRT
3251

purine or
Adenine
APRT
353

pyrimidine
phosphoribosyltransferase

metabolism
deficiency

Adenosine deaminase
ADA
100

deficiency

Adenosine monophosphate
AMPD1
270

deaminase deficiency type 1

Adenylosuccinate lyase
ADSL
158

deficiency

Dihydropyrimidine
DPYD
1806

dehydrogenase deficiency

Miller syndrome
DHODH
1723

Orotic aciduria
UMPS
7372

Purine nucleoside
PNP
4860

phosphorylase deficiency

Xanthinuria
XDH, MOCS1, MOCS2,
7498, 4337, 4338, 10243

GEPH

The Entrez entries listed in the table above are hereby incorporated by reference in their entireties.

Further, in some embodiments, the present methods and compositions find use in targeting any of the proteins or in treatment of any of the diseases or disorders of Table 2B. In various embodiments, the present invention contemplates the targeting of the full-length and/or truncated forms of any of the proteins disclosed in Table 2B. In various embodiments, the present invention contemplates the targeting of the precursor forms and/or mature forms and/or isoforms of any of the proteins disclosed in Table 2B.

In various embodiments, the present invention contemplates the targeting of a protein having about 60% (e.g. about 60%, or about 61%, or about 62%, or about 63%, or about 64%, or about 65%, or about 66%, or about 67%, or about 68%, or about 69%, or about 70%, or about 71%, or about 72%, or about 73%, or about 74%, or about 75%, or about 76%, or about 77%, or about 78%, or about 79%, or about 80%, or about 81%, or about 82%, or about 83%, or about 84%, or about 85%, or about 86%, or about 87%, or about 88%, or about 89%, or about 90%, or about 91%, or about 92%, or about 93%, or about 94%, or about 95%, or about 96%, or about 97%, or about 98%, or about 99%) sequence identity with any of the protein sequences disclosed herein (e.g. in Table 2B).

In various embodiments, the present invention contemplates the targeting of a protein comprising an amino acid sequence having one or more amino acid mutations relative to any of the protein sequences described herein (e.g. in Table 2B). For example, the present invention contemplates the targeting of a protein comprising an amino acid sequence having 1, or 2, or 3, or 4, or 5, or 6, or 7, or 8, or 9, or 10, or 11, or 12 amino acid mutations relative to any of the protein sequences described herein (e.g. in Table 2B). In some embodiments, the one or more amino acid mutations may be independently selected from substitutions, insertions, deletions, and truncations.

In some embodiments, the amino acid mutations are amino acid substitutions, and may include conservative and/or non-conservative substitutions.

“Conservative substitutions” may be made, for instance, on the basis of similarity in polarity, charge, size, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the amino acid residues involved. The 20 naturally occurring amino acids can be grouped into the following six standard amino acid groups: (1) hydrophobic: Met, Ala, Val, Leu, Ile; (2) neutral hydrophilic: Cys, Ser, Thr; Asn, Gln; (3) acidic: Asp, Glu; (4) basic: His, Lys, Arg; (5) residues that influence chain orientation: Gly, Pro; and (6) aromatic: Trp, Tyr, Phe.

As used herein, “conservative substitutions” are defined as exchanges of an amino acid by another amino acid listed within the same group of the six standard amino acid groups shown above. For example, the exchange of Asp by Glu retains one negative charge in the so modified polypeptide. In addition, glycine and proline may be substituted for one another based on their ability to disrupt α-helices.

As used herein, “non-conservative substitutions” are defined as exchanges of an amino acid by another amino acid listed in a different group of the six standard amino acid groups (1) to (6) shown above.

In various embodiments, the substitutions may also include non-classical amino acids (e.g. selenocysteine, pyrrolysine, N-formylmethionine β-alanine, GABA and δ-Aminolevulinic acid, 4-aminobenzoic acid (PABA), D-isomers of the common amino acids, 2,4-diaminobutyric acid, α-amino isobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, γ-Abu, ε-Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3-amino propionic acid, ornithine, norleucine, norvaline, hydroxyproline, sarcosme, citrulline, homocitrulline, cysteic acid, t-butylglycine, t-butylalanine, phenylglycine, cyclohexylalanine, β-alanine, fluoro-amino acids, designer amino acids such as β methyl amino acids, C α-methyl amino acids, N α-methyl amino acids, and amino acid analogs in general).

In Table 2B, all Illustrative Identifiers (e.g. Gene Seq nos. and references are hereby incorporated by reference in their entireties).

TABLE 2B

Illustrative Proteins, Illustrative Peptides, and Illustrative Indications

Protein/Peptide

Illustrative Identifier

Reference
Description/Illustrative Indication(s)

Transthyretin (TTR)
his gene encodes transthyretin, one of the three prealbumins including alpha-1-

(SEQ ID NOs: 637 and 638)
antitrypsin, transthyretin and orosomucoid. Transthyretin is a carrier protein; it

Gene ID: 7276
transports thyroid hormones in the plasma and cerebrospinal fluid, and also

transports retinol (vitamin A) in the plasma. The protein consists of a tetramer of

identical subunits. More than 80 different mutations in this gene have been reported;

most mutations are related to anyloid deposition, affecting predominantly peripheral

nerve and/or the heart, and a small portion of the gene mutations is non-

amyloidogenic. The diseases caused by mutations include amyloidotic polyneuropathy,

euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy,

oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis, carpal tunnel

syndrome, etc.

Endothelial Cell Specific
This gene encodes a secreted protein which is mainly expressed in the endothelial

Molecule 1
cells in human lung and kidney tissues. The expression of this gene is regulated by

(SEQ ID NO: 629 to 632)
cytokines, suggesting that it may play a role in endothelium-dependent pathological

Gene ID: 11082
disorders. The transcript contains multiple polyadenylation and mRNA instability

signals. Two transcript variants encoding different isoforms have been found for this gene

Parathyroid hormone
PTH is secreted by the chief cells of the parathyroid glands. PTH plays an important

P01270|PTHY_HUMAN
role in the regulation of serum calcium, serum phosphate, and vitamin D synthesis.

Parathyroid hormone

(SEQ ID NO: 508)

BMP-1 GeneSeq
BMP1 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession P80618
morphogenic proteins induce cartilage and bone formation, play important role in

WO8800205
nephrogesis, and play an important role in the development of many organs,

P13497/BMP1_HUMAN
including lung, heart, teeth, gut, skin, and particularly the kidney. BMP-1 activity can

Bone morphogenetic
be determined using the following assays known in the art: Nat Genet. 2001 January;

protein 1
27(1): 84-8; Eur J Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274,

(isoform BMP1-3)
Issue 16, 10897-10902, Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10,

(SEQ ID NO: 169)
1580-1594. Induction of Cartilage, Tissue and Bone Growth, and Diabetes

P13497-2|BMP1_HUMAN

Isoform BMP1-1 of Bone

morphogenetic protein 1

(isoform BMP1-1)

(SEQ ID NO: 509)

P13497-3|BMP1_HUMAN

Isoform BMP1-4 of Bone

morphogenetic protein 1

(isoform BMP1-4)

(SEQ ID NO: 510)

P13497-4|BMP1_HUMAN

Isoform BMP1-5 of Bone

morphogenetic protein 1

(isoform BMP1-5)

(SEQ ID NO: 511)

P13497-5|BMP1_HUMAN

Isoform BMP1-6 of Bone

morphogenetic protein 1

(isoform BMP1-6)

(SEQ ID NO: 512)

P13497-6|BMP1_HUMAN

Isoform BMP1-7 of Bone

morphogenetic protein 1

(isoform BMP1-7)

(SEQ ID NO: 513)

BMP-2 GeneSeq
BMP-2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession P80619
morphogenic protein induces bone formation. BMP-2 activity can be determined

WO8800205
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P12643/BMP2_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 2
Induction of Cartilage, Tissue and Bone Growth, and Diabetes. Infarction recovery.

(SEQ ID NO: 170)
Bone repair. Osteoporosis.

BMP-3
BMP-3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

P12645|BMP3_HUMAN
morphogenic protein induces bone formation. BMP-3 activity can be determined

Bone morphogenetic
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

protein 3
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

(SEQ ID NO: 514)
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

Induction of Cartilage, Tissue and Bone Growth, and Diabetes

BMP-2B GeneSeq
BMP-2b belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession W24850
morphogenic protein induces bone formation. BMP-2b activity can be determined

U.S. Pat. No. 5,631,142
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P12644/BMP4_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; I Biol Cbcre, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594. Induction of

protein 4
Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 171)

BMP-4 GeneSeq
BMP-4 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession B02796
morphogenic protein induces bone formation. BMP-4 activity can be determined

WO0020591
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P12644/BMP4_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 4
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 172)

BMP-5 GeneSeq
BMP-5 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession B02797
morphogenic protein induces bone formation. BMP-5 activity can be determined

WO0020591
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P22003/BMP5_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 5
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(isoform 1)

(SEQ ID NO: 173)

P22003-2|BMP5_HUMAN

Isoform 2 of Bone

morphogenetic protein 5

(isoform 2)

(SEQ ID NO: 515)

BMP-6 GeneSeq
BMP-6 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession R32904
morphogenic protein induces bone formation. BMP-6 activity can be determined

U.S. Pat. No. 5,187,076
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P22004/BMP6_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 6
Induction of Cartilage, Tissue and Bone Growth, and Diabetes. Hemochromatosis.

(SEQ ID NO: 174)

Osteogenic Protein-1; OP-
OP-1 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

1; BMP-7 GeneSeq
morphogenic protein induces bone formation. OP-1 activity can be determined using

Accession W34783
the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

WO973462
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

P18075/BMP7_HUMAN
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

Bone morphogenetic
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

protein 7

(SEQ ID NO: 175)

BMP7 Variant A
OP-1 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

(SEQ ID NO: 579)
morphogenic protein induces bone formation. OP-1 activity can be determined using

the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

Induction of Cartilage, Tissue and Bone Growth, and Diabetes

BMP7 Variant B
OP-1 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

(SEQ ID NO: 580)
morphogenic protein induces bone formation. OP-1 activity can be determined using

the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

Induction of Cartilage, Tissue and Bone Growth, and Diabetes

BMP7 Variant C
OP-1 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

(SEQ ID NO: 581)
morphogenic protein induces bone formation. OP-1 activity can be determined using

the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

Induction of Cartilage, Tissue and Bone Growth, and Diabetes

Osteogenic Protein-2
OP-2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

GeneSeq Accession
morphogenic protein induces bone formation. OP-2 activity can be determined using

R57973 WO9406399
the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

P34820/BMP8B_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 0, 1580-1594.

protein 8B
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 176)

GDF-1 GeneSeq
Members of the TGF-beta family of proteins initiate cell signaling by binding to

Accession R60961
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

WO9406449
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

P27539/GDF1_HUMAN
Cell Biol. 4: 172 178; Miyazono, K. et al., (1994) Adv. Immunol. 55: 181-220).

Embryonic
Activation of this heteromeric receptor complex occurs when TGF-beta binds to

growth/differentiation
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

factor 1
propagates the signal to downstream targets (Chen, F. and Weinberg, R. A. (1995)

(SEQ ID NO: 177)
PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341 347). The effect of

GDF-1 on signaling can be assayed by treating Primary BAECs transferred with a

construct called p3TP-Lux, containing a TGF-beta responsive promoter fused to a

reporter gene, and measuring luciferase gene expression (Wrana et al., 1994, Nature

370: 341-347). Developmental disorders, Induction of Cartilage, Tissue and Bone

Growth, and Diabetes

BMP-9 GeneSeq
BMP-9 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession R86903
morphogenic protein induces bone formation. BMP-9 activity can be determined

WO9533830
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

Q9UK05/GDF2_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Growth/differentiation
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

factor 2
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 178)

BMP-10 GeneSeq
BMP-10 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession R66202
morphogenic protein induces bone formation. BMP-10 activity can be determined

WO9426893
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

O95393/BMP10_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 10
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 179)

BMP-12 GeneSeq
BMP-12 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession R78734
morphogenic protein induces bone formation. BMP-12 activity can be determined

WO9516035
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

Q7Z4P5/GDF7_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Growth/differentiation
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

factor 7
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 180)

BMP-15 GeneSeq
BMP-15 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession W11261
morphogenic protein induces bone formation. BMP-15 activity can be determined

W09636710
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

O95972/BMP15_HUMAN
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

Bone morphogenetic
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

protein 15
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

(SEQ ID NO: 181)

BMP-17 GeneSeq
BMP-17 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession Y17870
morphogenic protein induces bone formation. BMP-17 activity can be determined

WO9929718
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

SEQ ID NO: 2 from
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

U.S. Pat. No. 7,151,086
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

(SEQ ID NO: 182)
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

BMP-18 GeneSeq
BMP-18 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone

Accession Y17871
morphogenic protein induces bone formation. BMP-18 activity can be determined

WO9929718
using the following assays known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J

SEQ ID NO: 4 from
Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902,

U.S. Pat. No. 7,151,086
Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594.

(SEQ ID NO: 183)
Induction of Cartilage, Tissue and Bone Growth, and Diabetes

Inhibin alpha GeneSeq
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion

Accession B02806
inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation

WO0020591
negatively and to have tumor-suppressor activity. In addition, serum levels of inhibin

P05111/INHA_HUMAN
have been shown to reflect the size of granulosa-cell tumors and can therefore be

Inhibin alpha chain
used as a marker for primary as well as recurrent disease. Tumor suppressor activity

(SEQ ID NO: 184)
of inhibin can be determined using assays known in the art: Matzuk et al., Nature

1992 Nov. 26: 360 (6402); 313-9. Tumor suppression.

Inhibin beta GeneSeq
The inhibin beta A subunit joins the alpha subunit to form a pituitary FSH secretion

Accession H02808
inhibitor. Inhibin has been shown to regulate gonadal stromal cell proliferation

WO0020591
negatively and to have tumour-suppressor activity. In addition, serum levels of inhibin

P08476/INHBA_HUMAN
have been shown to reflect the size of granulosa-cell tumors and can therefore be

Inhibin beta A chain
used as a marker for primary as well as recurrent disease. Tumor suppressor activity

(SEQ ID NO: 185)
of inhibin can be determined using assays known in the art: Matzuk et al., Nature

P09529/INHBB_HUMAN
1992 Nov. 26: 360 (6402); 313-9. Tumor suppression.

Inhibin beta B chain

(SEQ ID NO: 186)

Cerberus Protein
Cerebus is believed to be involved in the inhibition of BMP activity BMP activity, in the

GeneSeq Accession
presence of the antagonist Cerebus, can be determined using the following assays

W86032 WO9849296
known in the art: Nat Genet. 2001 January; 27(1): 84-8; Eur J Biochem 1996 Apr. 1;

O95813/CER1_HUMAN
237(1): 295-302; J Biol Chem, Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and

Cerberus
Hogan, B. L. M. (1996) Genes Dev. 10, 1580-1594. BMP Antagonist useful for

(SEQ ID NO: 187)
Osteosarcoma, abnormal bone growth.

Soluble BMP Receptor
Soluble BMP receptor kinase protein-3 is involved in the binding of BMPs. Soluble

Kinase Protein-3
BMP receptor kinase protein-3 is useful as an antagonist for the inhibition of BMP

GeneSeq Accession
activity. BMP activity, in the presence of the soluble antagonist BMP receptor kinase

R95227 WO9614579
protein-3, can be determined using the following assays known in the art: Nat Genet.

Q13873/BMPR2_HUMAN
2001 January; 27(1): 84-8; Eur J Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem,

Bone morphogenetic
Vol. 274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes

protein receptor type-2
Dev. 10, 1580-1594. BMP Antagonist useful for Osteosarcoma, abnormal bone

(SEQ ID NO: 188)
growth.

BMP Processing Enzyme
BMPs belong to the transforming growth factor-beta (TGFB) superfamily. Bone

Furin GeneSeq Accession
morphogenic protein induces bone formation. BMP activity, in the presence of the

W36099 WO9741250
Furin, can be determined using the following assays known in the art: Nat Genet.

P09958/FURIN_HUMAN
2001 January; 27(1): 84-8; Eur J Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol.

Furin
274, Issue 16, 10897-10902, Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Dev.

(SEQ ID NO: 189)
10, 1580-1594. Bone formation or Regeneration Abnormalities

TGF-beta 1 GeneSeq
Members of the TGF-beta family of proteins initiate cell signaling by binding to

Accession R29657
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

WO9216228
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

P01137/TGFB1_HUMAN
Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

Transforming growth
Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

factor beta-1
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

(SEQ ID NO: 190)
propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

betas on signaling can be assayed by treating Primary BAECs transfected with a

construct called p3TP-Lux, containing a TGF-beta responsive promoter fused to a

reporter gene, and measuring luciferase gene expression (Wrana et al., 1994, Nature

370: 341-347). Useful for treating cancer and to promote wound healing.

TGF-beta 2 GeneSeq
Members of the TGF-beta family of proteins initiate cell signaling by binding to

Accession R39659
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

EP542679
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

P61812/TGFB2_HUMAN
Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

Transforming growth
Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

factor beta-2
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

(SEQ ID NO: 191)
propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

betas on signaling can be assayed by treating Primary BAECs transfected with a

construct called p3TP-Lux, containing a TGF-beta responsive promoter fused to a

reporter gene, and measuring luciferase gene expression (Wrana et al., 1994, Nature

370: 341-347). Useful for treating cancer and to promote wound healing.

ZTGF-beta 9 GeneSeq
Members of the TGF-beta family of proteins initiate cell signaling by binding to

Accession Y70654
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

WO0015798
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

SEQ ID NO: 2 of
Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

WO0015798
Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

(SEQ ID NO: 192)
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

betas on signaling can be assayed by treating Primary BAECs transfected with a

construct called p3TP-Lux, containing a TGF-beta responsive promoter fused to a

reporter gene, and measuring luciferase gene expression (Wrana et al., 1994, Nature

370: 341-347). Useful for treating cancer and to promote wound healing.

Anti-TGF beta family
Members of the TGF-beta family of proteins initiate cell signaling by binding to

antibodies GB2305921
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

betas on signaling in the presence of an anti-TGF beta antibody, can be assayed by

treating Primary BAECs transfected with a construct called p3TP-Lux, containing a

TGF-beta responsive promoter fused to a reporter gene, and measuring luciferase

gene expression (Wrana et al., 1994, Nature 370: 341-347). Useful for control of

fibrosis, immune, and inflammatory disease.

Latent TGF beta binding
Members of the TGF-beta family of proteins initiate cell signaling by binding to

protein II GeneSeq
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

Accession Y70552
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

WO0012551
Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

Q14767/LTBP2_HUMAN
Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

Latent-transforming
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

growth factor beta-binding
propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

protein 2
PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

(SEQ ID NO: 193)
betas on signaling in the presence of a TGF beta binding protein, can be assayed by

treating Primary BAECs transfected with a construct called p3TP-Lux, containing a

TGF-beta responsivepromoter fused to a reporter gene, and measuring luciferase

gene expression (Wrana et al., 1994, Nature 370: 341-347). Useful for inhibiting

tissue or tumor growth.

MP52 GeneSeq
Members of the TGF-beta family of proteins initiate cell signaling by binding to

Accession W36100
heteromeric receptor complexes of type I (TbetaRI) and type II (TbetaRII)

WO9741250
serine/threonine kinase receptors (reviewed by Massague, J. et al. (1994) Trends

P43026/GDF5_HUMAN
Cell Biol. 4: 172 178; Miyazono, K. et al. (1994) Adv. Immunol. 55: 181-220).

Growth/differentiation
Activation of this heteromeric receptor complex occurs when TGF-beta. binds to

factor 5
TbetaRII, which then recruits and phosphorylates TbetaRI. Activated TbetaRI then

(SEQ ID NO: 194)
propagates the signal to downstream targets (Chen, F. and Weinberg. R. A. (1995)

PNA892: 1565-1569; Wrana, J. L. et al. (1994) Nature 370: 341. The effect of TGF

betas on signaling can be assayed by treating Primary BAECs transfected with a

construct called p3TP-Lux, containing a TGF-beta responsive promoter fused to a

reporter gene, and measuring luciferase gene expression (Wrana et al., 1994, Nature

370: 341-347). Bone formation or Regeneration Abnormalities

b57 Protein GeneSeq
BMPs are involved in the induction of bone formation. Specific antagonists are useful

Accession W69293
is preventing this activity from occurring. BMP activity, in the presence of b57 protein,

WO9837195
can be determined using the following assays known in the art: Nat Genet. 2001 January;

SEQ ID NO: 2 of
27(1): 84-8; Eur J Biochem 1996 Apr. 1; 237(1): 295-302; J Biol Chem, Vol. 274, Issue

WO9837195
16, 1089-10902, Apr. 16, 1999; and Hogan, B. L. M. (1996) Genes Deve. 10, 1580-1594.

(SEQ ID NO: 195)
BMP Antagonist useful for Osteosarcoma, abnormal bone growth.

Resistin GeneSeq
This gene belongs to the family defined by mouse FIZZI and FIZZ3/Resistin genes.

Accession W69293
The characteristic feature of this family is the C-terminal stretch of 10 cys residues

WO0064920
with identical spacing. The mouse homolog of this protein is secreted by adipocytes,

Q9HD89/RETN_HUMAN
may be the hormone potentially linking obesity to type II diabetes. Ability of resistin to

Resistin
influence type II diabetes can be determined using assays known in the art: Pontoglio

(isoform 1)
et al., J Clin Invest 1998 May 15; 101(10): 2215-22. Type II diabetes and Syndrome

(SEQ ID NO: 196)
X.

Galectin-4 GeneSeq
Galectins are a family of carbohydrate-binding proteins characterized by an affinity for

Accession W11841
beta-galactoside containing glycoconjugates. Ability of Galectin-4 polypeptides to

WO9703190
bind lactose can be determined using assays known in the art: Wada, et al., J Biol

P56470/LEG4_HUMAN
Chem 1997 Feb. 28; 272(9): 6078-86. Lactose intolerance.

Galectin-4

(SEQ ID NO: 197)

APM-I; ACRP-30;
ACPR30 gene is exclusively expressed in adipose tissue. ACRP30 is thought to

Famoxin GeneSeq
increase fatty acid oxidation by muscle tissue. Ability of ACRP30 polypeptides to

Accession Y71035
influence obesity and fat oxidation can be determined using assays known in the art:

W00026363
Fruebis et al., Proc Nat'l Acad Sci USA 2001 Feb. 13; 98(4): 2005-10. Obesity,

Q15848/ADIPO_HUMAN
Metabolic disorders, Lipid Metabolism; Hormone Secretion.

Adiponectin

(SEQ ID NO: 198)

ACRP-30 Homologue;
ACPR30 gene is exclusively expressed in adipose tissue. ACRP30 is thought to

Complement Component
increase fatty acid oxidation by muscle tissue. Ability of ACRP30 homologue

Clq C GeneSeq Accession
polypeptides to influence obesity and fat oxidation can be determined using assays

B30234 WO0063376
known in the art: Fruebis et al., Proc Nat'l Acad Sci USA 2001 Feb. 13; 98(4): 2005-10.

P02747/C1QC_HUMAN
Obesity, Metabolic disorders, Lipid Metabolism; Hormone Secretion.

Complement C1q

subcomponent subunit C

(SEQ ID NO: 199)

Calpain-10a GeneSeq
Calpain is believed to play a role in insulin secretion and insulin activity, and therefore

Accession Y79567
may be useful in the treatment of type II diabetes. Ability of Calpain-10 to influence

WO0023603
type II diabetes can be determined using assays known in the art: Pontoglio et al., J

Q9HC96/CAN10_HUMAN
Clin Invest 1998 May 15; 101(10): 2215-22. Diabetes mellitus; Regulation of Insulin

Calpain-10
secretory response; Insulin mediated glucose transport disorders.

(Isoform A)

(SEQ ID NO: 200)

Calpain-10b GeneSeq
Calpain is believed to play a role in insulin secretion and insulin activity, and therefore

Accession Y79568
may be useful in the treatment of type II diabetes. Ability of Calpain-10 to influence

WO0023603
type II diabetes can be determined using assays known in the art: Pontoglio et al., J

Q9HC96-
Clin Invest 1998 May 15; 101(10): 2215-22. Diabetes mellitus; Regulation of Insulin

2/CAN10_HUMAN Isoform
secretory response; Insulin mediated glucose transport disorders.

B of Calpain-10

(SEQ ID NO: 201)

Calpain-10c GeneSeq
Calpain is believed to play a role in insulin secretion and insulin activity, and therefore

Accession Y79569
may be useful in the treatment of type II diabetes. Ability of Calpain-10 to influence

WO0023603
type II diabetes can be determined using assays known in the art: Pontoglio et al., J

Q9HC96-
Clin Invest 1998 May 15; 101(10): 2215-22. Diabetes mellitus; Regulation of Insulin

3/CAN10_HUMAN Isoform
secretory response; Insulin mediated glucose transport disorders.

C of Calpain-10

(SEQ ID NO: 202)

PDGF-D GeneSeq
Vascular Endothelial Growth Factor. Proliferation assay using NR6R-3T3 cells

Accession Y71130
(Rizzino 1988 Cancer Res. 48: 4266). Wound Healing; Atherosclermis.

WO0027879

Q9GZP0/PDGFD_HUMAN

Platelet-derived growth

factor D

(isoform 1)

(SEQ ID NO: 203)

FasL GeneSeq Accession
Activities associated with apoptosis and immune system functions. Activity can be

Y28594 WO9936079
determined using Apoptosis assays known in the art: Walczak et al. (1996) EMBOJ

P48023/TNFL6_HUMAN
16: 5386-5397. Apoptosis-related disorders; Autoimmune disorders; Graft v-Host

Tumor necrosis factor
disorders.

ligand superfamily

member 6

(isoform 1)

(SEQ ID NO: 204)

Chondro modulin-like
Chondromodulin proteins are cartilage proteins thought to confer resistance to

protein GeneSeq
anglogeneis, and thus are useful as anti-angiogenic agents that may have utility in

Accession Y71262
combating cancer. Ability of Chondromodulin-like protein to inhibit vascularization can

WO0029579
be determined using assays known in the art: Hirakie et al., J Biol Chem 1997 Dec.

SEQ ID NO: 2 from
19; 272(51): 32419-26. Antianglogenic agent; Osteoblast proliferation stimulator;

WO0029579
prevents vascularization of cartilage tissue; Useful to treat cancer.

(SEQ ID NO: 370)

Patched GeneSeq
Patched is a tumour-suppressor receptor for Sonic hedgehog (shh), which is a protein

Accession W72969
that controls developmental patterning and growth. Ability of soluble Patched to bind

U.S. Pat. No. 5,837,538
to and inhibit the activities of shh can be determined using assays known in the art:

Q13635/PTC1_HUMAN
Stone et al., Nature 1996 Nov. 14; 384(6605): 129-34. Receptor for Hedgehog cellular

Protein patched homolog 1
proliferation signaling molecule. This receptor is useful as a means of preventing

(isoform L)
cellular proliferation via the shh signaling pathway, thus useful for cancers.

(SEQ ID NO: 205)

Patched-2 GeneSeq
Patched is a tumour-suppressor receptor for Sonic hedgehog (shh), which is a protein

Accession Y43261
that controls developmental patterning and growth. Ability of soluble Patched to bind

WO9953058
to and inhibit the activities of shh can be determined using assays known in the art:

Q9Y6C5/PTC2_HUMAN
Stone et al., Nature 1996 Nov. 14; 384(6605): 129-34. Receptor for Hedgehog cellular

Protein patched homolog 2
proliferation signaling molecule. This receptor is useful as a means of preventing

(isoform 1)
cellular proliferation via the shh signaling pathway, thus useful for cancers.

(SEQ ID NO: 206)

Maspin; Protease Inhibitor
Maspin is a member of the serpin family of serine protease inhibitors that is thought to

5 GeneSeq Accession
suppress tumor metastasis. The inhibitory effects of Maspin and other protease

R50938 WO9405804
inhibitors can be assayed using methods known in the art such as a labeled protease

P36952/SPB5_HUMAN
substrate, for example, Universal Protease Substrate (casein, resorufin-labeled):

Serpin B5
Roche Molecular Biochemicals, Cat. No. 1080733. Tumor suppressor which is down-

(isoform 1)
regulated in breast cancers. The maspin protein has tumour suppressing and

(SEQ ID NO: 207)
invasion suppressing activity.

Endostatin GeneSeq
Endostatin is believed to inhibit effects of capillary endothelial cell proliferation. The

Accession B28399
inhibitory effects of endostatin can be assayed using assays disclosed by Cao et al.

WO0064946
(1996) J. Biol. Chem. 271 29461-29467. Anti-angiogenic activity. Useful in the

P39060/COIA1_HUMAN
prevention and/or treatment of cancers.

Collagen alpha-1(XVIII)

chain

(isoform 1)

(SEQ ID NO: 208)

aFGF; FGF-1 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession P94037
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

EP298723
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P05230/FGF1_HUMAN
be useful as anti-cancer agents. Diabetes, Metabolic Disease, Obesity.

Fibroblast growth factor 1

(isoform 1)

(SEQ ID NO: 209)

bFGF; FGF-2 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession R06685
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

FR2642086
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P09038/FGF2_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 2

(isoform 1)

(SEQ ID NO: 210)

FGF-3; INT-2 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession R07824
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9503831
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P11487/FGF3_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 3

(SEQ ID NO: 211)

FGF-4; HST-1; HBGF-4
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

GeneSeq Accession
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

R07825 WO9503831
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P08620/FGF4_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 4

(isoform 1)

(SEQ ID NO: 212)

FGF-5 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession W22600
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9730155
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P12034/FGF5_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 5

(isoform long)

(SEQ ID NO: 213)

FGF-6; Heparin binding
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

secreted transforming
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

factor-2 GeneSeq
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

Accession R58555
be useful as anti-cancer agents.

EP613946

P10767/FGF6_HUMAN

Fibroblast growth factor 6

(SEQ ID NO: 214)

FGF-8 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession R80783
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9524928
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

P55075/FGF8_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 8

(isoform 8E)

(SEQ ID NO: 215)

FGF-9; Gila activating
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

factor GeneSeq Accession
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

R70822 WO9503831
and proliferation of cells, such as epithelial cells and keratinocytes. Hair growth.

P31371/FGF9_HUMAN
Antagonists may be useful as anti-cancer agents.

Fibroblast growth factor 9

(SEQ ID NO: 216)

FGF-12; Fibroblast growth
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

factor homologous factor-1
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

GeneSeq Accession
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

W06309 WO9635708
be useful as anti-cancer agents.

P61328/FGF12_HUMAN

Fibroblast growth factor 12

(isoform 1)

(SEQ ID NO: 217)

FGF-19 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession Y08582
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9927100
and proliferation of cells, such as epithelial cells and keratinocytes. Chronic liver

O95750/FGF19_HUMAN
disease. Primary biliary cirrhosis. Bile acid-induced liver damage. Antagonists may be

Fibroblast growth factor 19
useful as anti-cancer agents.

(SEQ ID NO: 218)

FGF-16 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession Y05474
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9918128
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

O43320/FGF16_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 16

(SEQ ID NO: 219)

FGF-18 GeneSeq
Fibroblast Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

Accession Y08590
Cancer Res. 48: 4266); Examples 23 and 39 disclosed herein. Promotion of growth

WO9927100
and proliferation of cells, such as epithelial cells and keratinocytes. Antagonists may

O76093/FGF18_HUMAN
be useful as anti-cancer agents.

Fibroblast growth factor 18

(SEQ ID NO: 220)

flt-3 ligand GeneSeq
Stem Cell Progenitor Chemokine activities can be determined using assays known in

Accession R67541
the art: Methods in Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited

EP627487
by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa,

P49771|FLT3L_HUMAN
NJ. Promotion of immune cell growth and/or differentiation.

Fms-related tyrosine

kinase 3 ligand

(isoform 1)

(SEQ ID NO: 221)

VEGF-110 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y69417
determined using assays known in the art, such as those disclosed in International

WO0013702
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 11 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0013702
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 222)

VEGF-121 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession B50432
determined using assays known in the art, such as those disclosed in International

WO0071713
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 2 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0071713
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 223)

VEGF-138 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y43483
determined using assays known in the art, such as those disclosed in International

WO9940197
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 4 of
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO99/40197
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 371)

VEGF-145 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y69413
determined using assays known in the art, such as those disclosed in International

WO0013702
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 4 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0013702
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 224)

VEGF-162 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y43484
determined using assays known in the art, such as those disclosed in International

W09940197
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 8 of
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO99/40197
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 372)

VEGF-165 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y69414
determined using assays known in the art, such as those disclosed in International

WO0013702
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 6 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0013702
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 225)

VEGF-182 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y43483
determined using assays known in the art, such as those disclosed in International

W09940197
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 6 of
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO99/40197
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 373)

VEGF-189 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y69415
determined using assays known in the art, such as those disclosed in International

WO0013702
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 8 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0013702
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 226)

VEGF-206 GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession Y69416
determined using assays known in the art, such as those disclosed in International

W00013702
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 10 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO0013702
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 227)

VEGF-D GeneSeq
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

Accession W53240
determined using assays known in the art, such as those disclosed in International

WO9807832
Publication No. WO0045835, for example. Promotion of growth and proliferation of

O43915/VEGFD_HUMAN
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

Vascular endothelial
angiogenic agents, and may be applicable for cancer.

growth factor D

(SEQ ID NO: 374)

VEGF-E; VEGF-X
Promotes the growth and/or proliferation of endothelial cells. VEGF activity can be

GeneSeq Accession
determined using assays known in the art, such as those disclosed in International

Y33679 WO9947677
Publication No. WO0045835, for example. Promotion of growth and proliferation of

SEQ ID NO: 2 from
cells, such as vascular endothelial cells. Antagonists may be useful as anti-

WO9947677
angiogenic agents, and may be applicable for cancer.

(SEQ ID NO: 228)

VEGF Receptor; KDR; flk-
Receptor for VEGF polypeptides VEGF activity, in the presence of flk-1 polypeptides,

1 GeneSeq Accession
can be determined using assays known in the art, such as those disclosed in

W69679 WO9831794
International Publication No. WO0045835, for example. VEGF Receptor. Fusion

P35968/VGFR2_HUMAN
protein with the extracellular domain is useful as an anti-angiogenic agent.

Vascular endothelial
Antagonists may be useful in the promotion of angiogenesis.

growth factor receptor 2

(isoform 1)

(SEQ ID NO: 229)

Soluble VEGF Receptor
Receptor for VEGF polypeptides VEGF activity, in the presence of VEGF Receptor

GeneSeq Accession
polypeptides, can be determined using assays known in the art, such as those

W47037
disclosed in International Publication No. WO0045835, for example. VEGF Receptor.

U.S. Pat. No. 5,712,380
Fusion protein with the extracellular domain is useful as an anti-angiogenic agent.

sVEGF-RI (FIG. 3) of
Antagonists may be useful in the promotion of angiogenesis.

U.S. Pat. No. 5,712,380

(SEQ ID NO: 442)

sVEGF-RII (FIG. 11) of

U.S. Pat. No. 5,712,380

(SEQ ID NO: 443)

sVEGF-RTMI (FIG. 15) of

U.S. Pat. No. 5,712,380

(SEQ ID NO: 444)

sVEGF-RTMII (FIG. 13) of

U.S. Pat. No. 5,712,380

(SEQ ID NO: 445)

flt-1 GeneSeq Accession
Receptor for VEGF polypeptides VEGF activity, in the presence of flt-1 polypeptides,

Y70751 WO0021560
can be determined using assays known in the art, such as those disclosed in

P17948/VGFR1_HUMAN
International Publication No. WO0045835, for example. VEGF Receptor. Fusion

Vascular endothelial
protein with the extracellular domain is useful as an anti-angiogenic agent.

growth factor receptor 1
Antagonists may be useful in the promotion of angiogenesis.

(isoform 1)

(SEQ ID NO: 230)

VEGF R-3; flt-4 GeneSeq
Receptor for VEGF polypeptides VEGF activity, in the presence of flt-4 polypeptides,

Accession B29047
can be determined using assays known in the art, such as those disclosed in

WO0058511
International Publication No. WO0045835, for example. VEGF Receptor. Fusion

P35916/VGFR3_HUMAN
protein with the extracellular domain is useful as an anti-angiogenic agent.

Vascular endothelial
Antagonists may be useful in the promotion of angiogenesis.

growth factor receptor 3

(isoform 1)

(SEQ ID NO: 231)

Neuropilin-1 GeneSeq
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

Accession Y06319
known in the art, such as those disclosed in International Publication No.

WO9929858
WO0045835, for example. Promotion of growth and proliferation of cells, such as

O14786/NRP1_HUMAN
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

Neuropilin-1
may be applicable for cancer.

(isoform 1)

(SEQ ID NO: 232)

Neuropilin-2 GeneSeq
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

Accession Y03618
known in the art, such as those disclosed in International Publication No.

WO9929858
WO0045835, for example. Promotion of growth and proliferation of cells, such as

O60462/NRP2_HUMAN
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

Neuropilin-2
may be applicable for cancer.

(isoform A22)

(SEQ ID NO: 233)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle troponin C
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

W22597 W09730085
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

P02585/TNNC2_HUMAN
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

Troponin C, skeletal

muscle

(SEQ ID NO: 234)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle troponin I
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

W18054 W09730085
angiogenesis can be determined using assays known in the art. Proc Natl Acad Sci

P48788/TNNI2_HUMAN
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

Troponin I, fast skeletal

muscle

(isoform 1)

(SEQ ID NO: 235)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle troponin T
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

W22599 W09730085
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

SEQ ID NO: 3 of
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

WO9730085

(SEQ ID NO: 236)

fragment. myofibrillar
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

protein troponin I
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

W18053 W09719955
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

SEQ ID NO: 3 of
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

WO9719955

(SEQ ID NO: 237)

myofibrillar protein
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

troponin I GeneSeq
may contribute to the difficulty encountered in revascularizing the ischemic

Accession W18054
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

WO9719955
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

SEQ ID NO: 3 of
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

WO9719955

(SEQ ID NO: 237)

Troponin peptides
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

GeneSeq Accessions
may contribute to the difficulty encountered in revascularizing the ischemic

Y29581, Y29582, Y29583,
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

Y29584, Y29585, and
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

Y29586 WO9933874
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

Wildtype troponins

provided as:

Human fast twitch skeletal

muscle troponin C

GeneSeq Accession

W22597 W09730085

P02585/TNNC2_HUMAN

Troponin C, skeletal

muscle

(SEQ ID NO: 234)

Human fast twitch skeletal

muscle troponin I

GeneSeq Accession

W18054 W09730085

P48788/TNNI2_HUMAN

Troponin I, fast skeletal

muscle

(isoform 1)

(SEQ ID NO: 235)

Human fast twitch skeletal

muscle troponin T

GeneSeq Accession

W22599 W09730085

SEQ ID NO: 3 of

WO9730085

(SEQ ID NO: 236)

fragment. myofibrillar

protein troponin I

GeneSeq Accession

W18053 W09719955

SEQ ID NO: 3 of

WO9719955

(SEQ ID NO: 237)

Human fast twitch skeletal

muscle Troponin subunit C

GeneSeq Accession

B00134 WO0054770

SEQ ID NO: 1 of

WO0054770

(SEQ ID NO: 375)

Human fast twitch skeletal

muscle Troponin subunit I

Protein GeneSeq

Accession B00135

WO0054770

SEQ ID NO: 2 of

WO0054770

(SEQ ID NO: 376)

Human fast twitch skeletal

muscle Troponin subunit T

GeneSeq Accession

B00136 WO0054770

SEQ ID NO: 3 of

WO0054770

(SEQ ID NO: 377)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle Troponin subunit C
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

B00134 WO0054770
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

SEQ ID NO: 1 of
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

WO0054770

(SEQ ID NO: 375)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle Troponin subunit I
may contribute to the difficulty encountered in revascularizing the ischemic

Protein GeneSeq
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

Accession B00135
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

WO0054770
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

SEQ ID NO: 2 of

WO0054770

(SEQ ID NO: 376)

Human fast twitch skeletal
Troponins are contractile proteins that are thought to inhibit angiogenesis. High levels

muscle Troponin subunit T
may contribute to the difficulty encountered in revascularizing the ischemic

GeneSeq Accession
myocardium after cardiovascular injury. Ability of soluble Troponins to inhibit

B00136 WO0054770
angiogenesis can be determined using assays known in the art:. Proc Natl Acad Sci

SEQ ID NO: 3 of
USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis

WO0054770

(SEQ ID NO: 377)

Activator Inbibitor-1; PAI-1
PAIs are believed to play a role in cancer, and cardiovascular disease and blood-

GeneSeq Accession
clotting disorders. Methods that measure plasminogen activator inhibitor (PAI) activity

R08411 WO9013648
are known in the art, for example, assay the ability of PAI to inhibit tissue

P05121/PAI1_HUMAN
plasminogen activator (tPA) or urokinase (uPA): J Biochem Biophys Methods 2000

Plasminogen activator
Sep. 11; 45(2): 127-40, Breast Cancer Res Treat 1996; 41(2): 141-6. Methods that

inhibitor 1
measure anti-angiogenesis activity are known in the art, for example, Proc Natl Acad

(isoform 1)
Sci USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis; blood-clotting disorders.

(SEQ ID NO: 238)

Plasminogen Activator
PAIs are believed to play a role in cancer, and cardiovascular disease and blood-

Inhibitor-2; PAI-2
clotting disorders. Methods that measure plasminogen activator inhibitor (PAI) activity

GeneSeq Accession
are known in the art, for example, assay the ability of PAI to inhibit tissue

P94160 DE3722673
plasminogen activator (tPA) or urokinase (uPA): J Biochem Biophys Methods 2000

P05120/PAI2_HUMAN
Sep. 11; 45(2): 127-40, Breast Cancer Res Treat 1996; 41(2): 141-6. Methods that

Plasminogen activator
measure anti-angiogenesis activity are known in the art, for example, Proc Natl Acad

inhibitor 2
Sci USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis; blood-clotting disorders.

(SEQ ID NO: 239)

Activator Inhibitor-2; PAI-2
PAIs are believed to play a role in cancer, and cardiovascular disease and blood-

GeneSeq Accession
clotting disorders. Methods that measure plasminogen activator inhibitor (PAI) activity

R10921 WO9102057
are known in the art, for example, assay the ability of PAI to inhibit tissue

P05120/PAI2_HUMAN
plasminogen activator (tPA) or urokinase (uPA): J Biochem Biophys Methods 2000

Plasminogen activator
Sep. 11; 45(2): 127-40, Breast Cancer Res Treat 1996; 41(2): 141-6. Methods that

inhibitor 2
measure anti-angiogenesis activity are known in the art, for example, Proc Natl Acad

(SEQ ID NO: 239)
Sci USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis; blood-clotting disorders.

Human PAI-1 mutants
PAIs are believed to play a role in cancer, and cardiovascular disease and blood-

GeneSeq Accessions
clotting disorders. Methods that measure plasminogen activator inhibitor (PAI) activity

R11755, R11756, R11757,
are known in the art, for example, assay the ability of PAI to inhibit tissue

R11758, R11759, R11760,
plasminogen activator (tPA) or urokinase (uPA): J Biochem Biophys Methods 2000

R11761, R11762 and
Sep. 11; 45(2): 127-40, Breast Cancer Res Treat 1996; 41(2): 141-6. Methods that

R11763 WO9105048
measure anti-angiogenesis activity are known in the art, for example, Proc Natl Acad

Wildtype PAI-1 is provided
Sci USA 1999 Mar. 16; 96(6): 2645-50. Anti-angiogenesis; blood-clotting disorders.

as P05121/PAI1_HUMAN

Plasminogen activator

inhibitor 1

(isoform 1)

(SEQ ID NO: 238)

CXCR3; CXC GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession Y79372
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO0018431
Members of this family are involved in a similarly diverse range of pathologies

P49682|CXCR3_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-X-C chemokine receptor
chemokines exert their effects by acting on a family of seven transmembrane G-

type 3
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform 1)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 240)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Soluble CXCR3 polypeptides may be useful for

inhibiting chemokine activities and viral infection.

Modified Rantes GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W38129
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9737005
Members of this family are involved in a similarly diverse range of pathologies

Wildtype Rantes provided
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

herein as
chemokines exert their effects by acting on a family of seven transmembrane G-

P13501/CCL5_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 5
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 241)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

RANTES GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession Y05299
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

EP905240
Members of this family are involved in a similarly diverse range of pathologies

P13501/CCL5_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 5
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 241)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

MCP-Ia GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession R73914
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9509232
Members of this family are involved in a similarly diverse range of pathologies

MCP-1 provided as
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P13500/CCL2_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 2
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 337)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

MCP-Ib GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession Y26176
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9929728
Members of this family are involved in a similarly diverse range of pathologies

MCP-1 provided as
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P13500/CCL2_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 2
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 337)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

MCP-I receptor GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession R79165
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9519436
Members of this family are involved in a similarly diverse range of pathologies

MCP-IA
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9519436
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 446)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

MCP-1B
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

SEQ ID NO: 4 of
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

WO9519436
Humana Press Inc., Totowa, NJ. Soluble MCP-1 Receptor polypeptides may be

(SEQ ID NO: 447)
useful for inhibiting chemokine activities and viral infection.

MCP-3 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession R73915
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W09509232
Members of this family are involved in a similarly diverse range of pathologies

P80098/CCL7_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 7
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 336)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

MCP-4 receptor GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W56689
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9809171
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 2 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9809171
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 378)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Soluble MCP-4 Receptor polypeptides may be

useful for inhibiting chemokine activities and viral infection.

RANTES receptor
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W29588
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 5,652,133
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

U.S. Pat. No. 5,652,133
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 379)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Soluble RANTES Receptor polypeptides may be

useful for inhibiting chemokine activities and viral infection.

CCR5 variant GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W88238
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9854317
Members of this family are involved in a similarly diverse range of pathologies

Variants of wildtype CCR5
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

which has the sequence
chemokines exert their effects by acting on a family of seven transmembrane G-

of:
protein coupled receptors. Over 40 human chemokines have been described, which

P51681|CCR5_HUMAN
bind to ~17 receptors thus far identified. Chemokine activities can be determined

C-C chemokine receptor
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

type 5
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 448)
Humana Press Inc., Totowa, NJ. Soluble CCR5 polypeptides may be useful for

inhibiting chemokine activities and viral infection.

CCR7 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession B50859
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

U.S. Pat. No. 6,153,441
Members of this family are involved in a similarly diverse range of pathologies

P32248/CCR7_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C chemokine receptor
chemokines exert their effects by acting on a family of seven transmembrane G-

type 7
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 243)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Soluble CCR7 polypeptides may be useful for

inhibiting chemokine activities and viral infection.

CXC3 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W23345
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9727299
Members of this family are involved in a similarly diverse range of pathologies

P78423/X3CL1_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Fractalkine
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 244)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

Eotaxin GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W10099
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9700960
Members of this family are involved in a similarly diverse range of pathologies

P51671/CCL11_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Eotaxin
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 245)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

Neurotactin GeneSeq
Neurotactin may play a role in chemotactic leukocyte migration and brain

Accessions Y77537,
inflammation processes. Chemotactic leukocyte migration assays are known in the

W34307, Y53259, and,
art, for example: J. Immunol. Methods 33, ((1980)); Nature 1997 Jun. 5; 387(6633):

Y77539 U.S. Pat. No.
611-7. Immune disorders.

6,013,257 WO9742224

P78423/X3CL1_HUMAN

Fractalkine

(SEQ ID NO: 244)

Human CKbeta-9
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B50860
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 6,153,441
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

U.S. Pat. No. 6,153,441
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 246)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

Lymphotactin GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession B50052
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO0073320
Members of this family are involved in a similarly diverse range of pathologies

P47992/XCL1_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Lymphotactin
chemokines exert their effects by acting on a family of seven transmembrane G.

(SEQ ID NO: 247)
Chemokine activities can be determined using assays known in the art: Methods in

Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

Immune disorders.

MIP-3 alpha GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W44398
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9801557
Members of this family are involved in a similarly diverse range of pathologies

P78556/CCL20_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 20
chemokines exert their effects by acting on a family of seven transmembrane G.

(isoform 1)
Chemokine activities can be determined using assays known in the art: Methods in

(SEQ ID NO: 248)
Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

Immune disorders.

MIP-3 beta GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W44399
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9801557
Members of this family are involved in a similarly diverse range of pathologies

Q99731/CCL19_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 19
chemokines exert their effects by acting on a family of seven transmembrane G.

(SEQ ID NO: 249)
Chemokine activities can be determined using assays known in the art: Methods in

Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

Immune disorders.

MIP-Gamma GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession R70798
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO2006135382
Members of this family are involved in a similarly diverse range of pathologies

(SEQ ID NO: 457)
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokines exert their effects by acting on a family of seven transmembrane G.

Chemokine activities can be determined using assays known in the art: Methods in

Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

Immune disorders.

Stem Cell Inhibitory Factor
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

R11553 WO9104274
Members of this family are involved in a similarly diverse range of pathologies

SCIF in Table I of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9104274
chemokines exert their effects by acting on a family of seven transmembrane G.

(SEQ ID NO: 380)
Chemokine activities can be determined using assays known in the art: Methods in

SCIF in Table II of
Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

WO9104274
T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

(SEQ ID NO: 381)
Hematopoietic growth factors.

Thrombopoietin GeneSeq
Thrombopoietin is involved in the regulation of the growth and differentiation of

Accession R79905
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

WO9521920
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

P40225|TPO_HUMAN
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Thrombopoietin
Hematopoietic growth factors.

(isoform 1)

(SEQ ID NO: 250)

c-kit ligand; SCF; Mast cell
C-kit ligan is thought to stimulate the proliferation of mast cells, and is able to

growth factor; MGF;
augment the proliferation of both myeloid and lymphoid hematopoietic progenitors in

Fibrosarcoma- derived
bone marrow culture. C-kit ligand is also though to act synergistically with other

stem cell factor GeneSeq
cytokines. Chemokine activities can be determined using assays known in the art:

Accession Y53284,
Methods in Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I. Proudfoot,

R83978 and R83977
T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

EP992579 and EP676470
Hematopoietic growth factors.

P21583|SCF_HUMAN Kit

ligand

(isoform 1)

(SEQ ID NO: 251)

Platelet derived growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

factor GeneSeq Accession
known in the art, such as those disclosed in International Publication No.

B48653 WO0066736
WO0045835, for example. Promotion of growth and proliferation of cells, such as

PDGF-A
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

P04085/PDGFA_HUMAN
may be applicable for cancer.

Platelet-derived growth

factor subunit A

(Isoform long)

(SEQ ID NO: 257)

PDGF-B

P01127/PDGFB_HUMAN

Platelet-derived growth

factor subunit B

(isoform 1)

(SEQ ID NO: 258)

Melanoma inhibiting
Melanoma inhibiting protein has melanoma-inhibiting activity and can be used to treat

protein GeneSeq
cancer (melanoma, glioblastoma, neuroblastoma, small cell lung cancer,

Accession R69811
neuroectodermal tumors) or as an immunosuppressant (it inhibits IL-2 or

WO9503328
phytohaemagglutinin induced proliferation of peripheral blood lymphocytes. Tumor

(SEQ ID NO: 458)
suppressor activity of melanoma inhibiting protein can be determined using assays

known in the art: Matzuk et al., Nature 1992 Nov. 26; 360(6402): 313-9. Cancer;

melanoma

Glioma-derived growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

factor GeneSeq Accession
known in the art, such as those disclosed in International Publication No.

R08120 EP399816
WO0045835, for example. Promotion of growth and proliferation of cells, such as

vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

may be applicable for cancer.

Platelet derived growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

factor precursor A
known in the art, such as those disclosed in International Publication No.

GeneSeq Accession
WO0045835, for example. Promotion of growth and proliferation of cells, such as

R84759 EP682110
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

PDGF-A precursor (variant
may be applicable for cancer.

D1)

(SEQ ID NO: 382)

PDGF-A precursor (variant

13-1)

(SEQ ID NO: 383)

Platelet derived growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

factor precursor B
known in the art, such as those disclosed in International Publication No.

GeneSeq Accession
WO0045835, for example. Promotion of growth and proliferation of cells, such as

R84760 EP682110, FIG.
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

1 or FIG. 2
may be applicable for cancer.

Wildtype PDGF-B

provided as:

PDGF-B

P01127/PDGFB_HUMAN

Platelet-derived growth

factor subunit B

(isoform 1)

(SEQ ID NO: 258)

Platelet derived growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

factor Bvsis GeneSeq
known in the art, such as those disclosed in International Publication No.

Accession P80595 and
WO0045835, for example. Promotion of growth and proliferation of cells, such as

P80596 EP282317
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

FIG. 1 of EP282317
may be applicable for cancer.

(SEQ ID NO: 384)

Placental Growth Factor
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

GeneSeq Accessions
known in the art, such as those disclosed in International Publication No.

R23059 and R23060
WO0045835, for example. Promotion of growth and proliferation of cells, such as

WO9206194
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

P49763-2/PLGF_HUMAN
may be applicable for cancer.

Isoform PIGF-1 of

Placenta growth factor

(isoform PIGF-1)

(SEQ ID NO: 252)

Placental Growth Factor-2
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

GeneSeq Accession
known in the art, such as those disclosed in International Publication No.

Y08289 DE19748734
WO0045835, for example. Promotion of growth and proliferation of cells, such as

P49763-3/PLGF_HUMAN
vascular endothelial cells. Antagonists may be useful as anti-angiogenic agents, and

Isoform PIGF-2 of
may be applicable for cancer.

Placenta growth factor

(isoform PIGF-2)

(SEQ ID NO: 253)

Thrombopoietin
Thrombopoietin is involved in the regulation of the growth and differentiation of

derivative1 GeneSeq
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Accession Y77244
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin
Thrombopoietin is involved in the regulation of the growth and differentiation of

derivative2 GeneSeq
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Accession Y77255
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin derivative
Thrombopoietin is involved in the regulation of the growth and differentiation of

3 GeneSeq Accession
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Y77262
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin derivative
Thrombopoietin is involved in the regulation of the growth and differentiation of

4 GeneSeq Accession
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Y77267
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin derivative
Thrombopoietin is involved in the regulation of the growth and differentiation of

5 GeneSeq Accession
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Y77246
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin derivative
Thrombopoietin is involved in the regulation of the growth and differentiation of

6 GeneSeq Accession
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Y77253
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Thrombopoietin derivative
Thrombopoietin is involved in the regulation of the growth and differentiation of

7 GeneSeq Accession
megakaryocytes and preceptors thereof. Thrombopoietin (TPO) can be assayed to

Y77256
determine regulation of growth and differentiation of megakaryocytes. Mol Cell Biol

WO0000612 (e.g. Table 3)
2001 April; 21(8): 2659-70; Exp Hematol 2001 January; 29(1): 51-8 and within.

Wildtype thrombopoietin
Thrombocytopenia, cancer.

provided as:

P40225|TPO_HUMAN

Thrombopoietin

(isoform 1)

(SEQ ID NO: 250)

Fractalkine GeneSeq
Fractalkine is believed to play a role in chemotactic leukocyte migration and

Accession Y53255
neurological disorders. Fractalkine activity can be determined using Chemotactic

U.S. Pat. No. 6,043,086
leukocyte migration assays known in the art, for example: J. Immunol. Methods 33,

P78423/X3CL1_HUMAN
((1980)); Nature 1997 Jun. 5; 387(6633): 611-7. Immune disorders.

Fractalkine

(SEQ ID NO: 244)

CXC3 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W23345
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9757599
Members of this family are involved in a similarly diverse range of pathologies

P78423/X3CL1_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Fractalkine
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 244)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders.

CCR7 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession B50859
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

U.S. Pat. No. 6,153,441
Members of this family are involved in a similarly diverse range of pathologies

P32248/CCR7_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C chemokine receptor
chemokines exert their effects by acting on a family of seven transmembrane G-

type 7
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 243)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Soluble CCR7 polypeptides may be useful for

inhibiting chemokine activities and viral infection.

Nerve Growth Factor-beta
Nerve Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988 Cancer

GeneSeq Accession
Res. 48: 4266) Neurological disorders, cancer

R11474 EP414151

P01138/NGF_HUMAN

Beta-nerve growth factor

(SEQ ID NO: 254)

Nerve Growth Factor-
Nerve Growth Factor Proliferation assay using NR6R 3T3 cells (Rizzino 1988 Cancer

beta2 GeneSeq Accession
Res. 48: 4266 Neurological disorders, cancer

W69725 EP859056

FIG. 1 of EP859056

(SEQ ID NO: 465)

Neurotrophin-3 GeneSeq
Neurotrophins regulate neuronal cell survival and synaptic plasticity. Trk tyrosine

Accession W8889
kinase activation assays known in the art can be used to assay for neurotrophin

WO9821234
activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

P20783/NTF3_HUMAN
Neurological disorders, cancer

Neurotrophin-3

(isoform 1)

(SEQ ID NO: 255)

Neurotrophin-4 GeneSeq
Neurotrophins regulate neuronal cell survival and synaptic plasticity. Trk tyrosine

Accession R47100
kinase activation assays known in the art can be used to assay for neurotrophin

WO9325684
activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

P34130/NTF4_HUMAN
Neurological disorders, cancer

Neurotrophin-4

(SEQ ID NO: 256)

Neurotrophin- 4a
Neurotrophins regulate neuronal cell survival and synaptic plasticity. Trk tyrosine

GeneSeq Accession
kinase activation assays known in the art can be used to assay for neurotrophin

R47101 WO9325684
activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

Wildtype neurotrophin
Neurological disorders, cancer

provided as:

P34130/NTF4_HUMAN

Neurotrophin-4

(SEQ ID NO: 256)

Neurotrophin- 4b
Neurotrophins regulate neuronal cell survival and synaptic plasticity. tyrosine kinases.

GeneSeq Accession
Trk tyrosine kinase activation assays known in the art can be used to assay for

R47102 WO9325684
neurotrophin activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

P34130/NTF4_HUMAN
Neurological disorders, cancer

Neurotrophin-4

(SEQ ID NO: 256)

Neurotrophin- 4c
Neurotrophins regulate neuronal cell survival and synaptic plasticity. tyrosine kinases.

GeneSeq Accession
Trk tyrosine kinase activation assays known in the art can be used to assay for

R47103 WO9325684
neurotrophin activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

P34130/NTF4_HUMAN
Neurological disorders, cancer

Neurotrophin-4

(SEQ ID NO: 256)

Neurotrophin- 4d
Neurotrophins regulate neuronal cell survival and synaptic plasticity. tyrosine kinases.

GeneSeq Accession
Trk tyrosine kinase activation assays known in the art can be used to assay for

R47102 WO9325684
neurotrophin activity, for example, Proc Natl Acad Sci USA 2001 Mar. 13; 98(6): 3555-3560.

P34130/NTF4_HUMAN
Neurological disorders, cancer

Neurotrophin-4

(SEQ ID NO: 256)

Platelet-Derived Growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

Factor A chain GeneSeq
known in the art, such as those disclosed in International Publication No.

Accession R38918
W00045835, for example. Promotion of growth and proliferation of cells, such as

U.S. Pat. No. 5,219,739
vascular endothelial cells. Hematopoietic and immune disorders. Antagonists may

P04085/PDGFA_HUMAN
be useful as anti-angiogenic agents, and may be applicable for cancer

Platelet-derived growth

factor subunit A

(Isoform long)

(SEQ ID NO: 257)

Platelet-Derived Growth
Vascular Endothelial Growth Factor VEGF activity can be determined using assays

Factor B chain GeneSeq
known in the art, such as those disclosed in International Publication No.

Accession R38919
W00045835, for example. Promotion of growth and proliferation of cells, such as

U.S. Pat. No. 5,219,739
vascular endothelial cells. Hematopoietic and immune disorders. Antagonists may

P01127/PDGFB_HUMAN
be useful as anti-angiogenic agents, and may be applicable for cancer

Platelet-derived growth

factor subunit B

(isoform 1)

(SEQ ID NO: 258)

Stromal Derived Factor- 1
Stromal Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

alpha GeneSeq Accession
Cancer Res. 48: 4266) Hematopoietic, immune disorders, cancer

Y39995 WO9948528

P48061-2/SDF1_HUMAN

Isoform Alpha of Stromal

cell-derived factor 1

(isoform alpha)

(SEQ ID NO: 259)

Stromal Derived Factor- 1
Stromal Growth Factor Proliferation assay using NR6R-3T3 cells (Rizzino 1988

beta GeneSeq Accession
Cancer Res. 48: 4266) Hematopoietic, immune disorders, cancer

R75420 CA2117953

P48061/SDF1_HUMAN

Stromal cell-derived factor 1

(isoform beta)

(SEQ ID NO: 260)

Tarc GeneSeq Accession
Chemotactic for T lymphocytes. May play a role in T-cell development. Thought to

W14917 WO9711969
bind CCR8 and CCR4 Chemotactic leukocyte migration assays are known in the art,

Q92583/CCL17_HUMAN
for example: J. Immunol. Methods 33 ((1980)) Antiinflammatory. Immune disorders,

C-C motif chemokine 17
cancer

(SEQ ID NO: 261)

Prolactin GeneSeq
Prolactin is involved in immune cell proliferation and apoptosis. Immune coil

Accession R78691
proliferation and suppression of apoptosis by prolactin can be assayed by methods

WO9521625
well-known in the art, for example, Buckley, A R and Buckley D J, Ann NY Acad Sci

P01236/PRL_HUMAN
2000; 917: 522-33, and within. Reproductive system disorders, cancer.

Prolactin

(SEQ ID NO: 262)

Prolactin2 GeneSeq
Prolactin is involved in immune cell proliferation and apoptosis. Immune coil

Accession Y31764
proliferation and suppression of apoptosis by prolactin can be assayed by methods

U.S. Pat. No. 5,955,346
well-known in the art, for example, Buckley, A R and Buckley D J, Ann NY Acad Sci

2000; 917: 522-33, and within. Reproductive system disorders, cancer.

Follicle stimulating
FSH stimulates secretion of interleukin-1 by cells isolated from women in the follicular

hormone Alpha subunit
phase FSH activities can be determined using assays known in the art; J Gend Specif

GeneSeq Accession
Med 1999 November-December; 2(6): 30-4; Mol Cell Endocrinol. 1997 Nov. 15; 134(2): 109-18.

Y54160 EP974359
Reproductive system disorders, cancer.

P01215/GLHA_HUMAN

Glycoprotein hormones

alpha chain

(SEQ ID NO: 263)

Follicle stimulating
FSH stimulates secretion of interleukin-1 by cells isolated from women in the follicular

hormone Beta subunit
phase FSH activities can be determined using assays known in the art; J Gend Specif

GeneSeq Accession
Med 1999 November-December; 2(6): 30-4; Mol Cell Endocrinol. 1997 Nov. 15; 134(2): 109-18.

Y54161 EP974359
Reproductive system disorders, cancer.

P01225/FSHB_HUMAN

Follitropin subunit beta

(SEQ ID NO: 264)

Substance P (tachykinin)
Substance P is associated with immunoregulation. Immuneregulation and bone

GeneSeq Accession
marrow, cell proliferation by substance P can be assayed by methods well-known in

B23027 WO0054053
the art, for example, Lai et al. Proc Natl Acad Sci USA 2001 Mar. 27; 98(7): 3970-5;

(SEQ ID NO: 385)
Jallat-Daloz et al. Allergy Asthma Proc 2001 January-February; 22(1): 17-23; Kehler et al. Exp

Lung Res 2001 January-February; 27(1): 25-46; and Adamus M A and Dabrowski Z J. J Cell

Biochem 2001; 81(3)499-506. diabetes mellitus, hypertension, cancer

Oxytocin (Neurophysin I)
Oxytocin is involved in the induction of prostaglandin (E2) release as well as an

GeneSeq Accession
increased amount of calcium release by smooth muscle cells. Oxytocin and

B24085 and B24086
prostaglandin E(2) release and Ocytocin (Ca2+) increase can be assayed by

WO0053755
methods well-known in the art, for example, Pavan et al., AM J Obset Gynecol 2000

P01178/NEU1_HUMAN
July; 183(1): 76-82 and Holda et al., Cell Calcium 1996 July; 20(1): 43 51. inflammatory

Oxytocin-neurophysin 1
disorders immunologic disorders, cancer

(SEQ ID NO: 265)

Vasopressin (Neurophysin
Vasopressinis believed to have a direct antidiuretic action on the kidney, and it is

II) GeneSeq Accession
thought to cause vasoconstriction of the peripheral vessels. Vasopressin activity can

B24085 and B24086
be determined using assays known in the art, for example, Endocr Regul 1996 March;

WO0053755
30(I): 13-17. inflammatory disorders immunologic disorders, cancer

P01185/NEU2_HUMAN

Vasopressin-neurophysin

2-copeptin

(SEQ ID NO: 266)

IL-1 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

P60326 EP165654
monocytes, and macrophages. Known functions include stimulating proliferation of

IL-1 alpha
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P01583|IL1A_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-1 alpha
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 269)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

IL-1 beta
D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

P01584|IL1B_HUMAN
inflammatory disorders immunologic disorders, cancer

Interleukin-1 beta

(SEQ ID NO: 267)

IL-1 mature GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R14855
monocytes, and macrophages. Known functions include stimulating proliferation of

EP456332
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

(mature truncated form
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

wherein the precursor is
can be determined using assays known in the art: Matthews et al., in Lymphokines

cleaved between amino

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

acids 116-117)
D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

(SEQ ID NO: 386)
inflammatory disorders immunologic disorders, cancer

IL-1 beta GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession Y08322
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9922763
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P01584|IL1B_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-1 beta
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 267)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

IL-3 variants GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession P80382,
monocytes, and macrophages. Known functions include stimulating proliferation of

P80383, P80384, and
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P80381 WO8806161
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Variants of wildtype IL-3
can be determined using assays known in the art: Matthews et al., in Lymphokines

which has the sequence:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

P08700|IL3_HUMAN
D.C. 1987, pp. 221-225; and Kitamura et al (1989) J Cell Physiol. 140 323-334.

Interleukin-3
inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 449)

IL-4 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

P70615 WO8702990
monocytes, and macrophages. Known functions include stimulating proliferation of

P05112/IL4_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-4
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(isoform 1)
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 268)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

287-295. inflammatory disorders immunologic disorders, cancer

IL-4 muteins GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession W52151
monocytes, and macrophages. Known functions include stimulating proliferation of

W52152 W52153 W52154
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W52155 W52156 W52157
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

W52158 W52159 W52160
can be determined using assays known in the art: Matthews et al., in Lymphokines

W52161 W52162 W52163

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

W52164 and W52165
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

WO9747744
287-295. inflammatory disorders immunologic disorders, cancer

Variants of wildtype IL-4

which has the sequence:

P05112/IL4_HUMAN

Interleukin-4

(isoform 1)

(SEQ ID NO: 268)

IL-1 alpha GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession P90108
monocytes, and macrophages. Known functions include stimulating proliferation of

EP324447
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P01583|IL1A_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-1 alpha
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 269)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

IL-3 variants GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R38561,
monocytes, and macrophages. Known functions include stimulating proliferation of

R38562, R38563, R38564,
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

R38565, R38566, R38567,
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

R38568, R38569, R38570,
can be determined using assays known in the art: Matthews et al., in Lymphokines

R38571, and R38572

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

WO9307171
D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

Variants of wildtype IL-3
inflammatory disorders immunologic disorders, cancer

which has the sequence:

P08700|IL3_HUMAN

Interleukin-3

(SEQ ID NO: 449)

IL-6 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R45717 and R45718
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9402512
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P05231/IL6_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-6
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 270)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

inflammatory disorders immunologic disorders, cancer. Obesity. Metabolic Disease.

Diabetes.

IL-13 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R48624 WO9404680
monocytes, and macrophages. Known functions include stimulating proliferation of

P35225/IL13_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-13
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(SEQ ID NO: 271)
can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Boutelier et al (1995) J. Immunol. Methods 181, 29.

inflammatory disorders immunologic disorders, cancer

IL-4 mutein GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R47182
monocytes, and macrophages. Known functions include stimulating proliferation of

DE4137333
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Variants of wildtype IL-4
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

which has the sequence:
can be determined using assays known in the art: Matthews et al., in Lymphokines

P05112/IL4_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-4
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

(isoform 1)
287-295. inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 268)

IL-4 mutein Y124X
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R47183 DE4137333
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Variants of wildtype IL-4
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

which has the sequence:
can be determined using assays known in the art: Matthews et al., in Lymphokines

P05112/IL4_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-4
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

(isoform 1)
287-295. inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 268))

IL-4 mutein Y124G
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R47184 DE4137333
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Variants of wildtype IL-4
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

which has the sequence:
can be determined using assays known in the art: Matthews et al., in Lymphokines

P05112/IL4_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-4
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

(isoform 1)
287-295. inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 268)

Human Interleukin-10
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

(precursor) GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R41664
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9317698
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P22301/IL10_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-10

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(precursor form is
D.C. 1987, pp. 221-225; and Thompson-Snipes et al (1991) J. Exp. Med. 173, 507-510.

processed into a truncated
inflammatory disorders immunologic disorders, cancer

mature form)

(SEQ ID NO: 272)

Human Interleukin-10
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R42642 WO9318783-A
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 3 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9318783-A
can be determined using assays known in the art: Matthews et al., in Lymphokines

(mature IL-10)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 273)
D.C. 1987, pp. 221-225; and Thompson-Snipes et al (1991) J. Exp. Med. 173, 507-510.

inflammatory disorders immunologic disorders, cancer

Human interleukin-1 beta
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

precursor. GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R42447
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP569042
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P01584/IL1B_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-1 beta

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 274)
D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

Interleukin- 1alpha
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R45364 EP578278
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P01583|IL1A_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-1 alpha
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 269)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Human interleukin-3
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

variant GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R22814
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

JP04063595
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Variants of wildtype IL-3
can be determined using assays known in the art: Matthews et al., in Lymphokines

which has the sequence:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

P08700|IL3_HUMAN
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334.

Interleukin-3
inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 449)

IL-1i fragments GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R35484 and
monocytes, and macrophages. Known functions include stimulating proliferation of

R35485 EP541920
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

IL-1 inhibitor (IL-li)
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R35486 and R35484
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EPS541920
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarclio (1989) inflammatory disorders

immunologic disorders, cancer

ICE 22 kD subunit.
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R33780 EP533350
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 16 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

EP533350
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 450)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

ICE 20 kD subunit.
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R33781 EP533350
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 17 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

EP533350
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 451)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

ICE 10 kD subunit
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R33782 EP533350
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 18 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

EP533350
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 452)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Human Interleukin-10
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

(precursor) GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R41664
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9317698
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P22301/IL10_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-10

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(precursor form is
D.C. 1987, pp. 221-225; and Thompson-Snipes et al (1991) J. Exp. Med. 173, 507-510.

processed into a truncated
inflammatory disorders immunologic disorders, cancer

mature form)

(SEQ ID NO: 272)

Human Interleukin-10
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R42642 WO9318783
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 3 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9318783-A
can be determined using assays known in the art: Matthews et al., in Lymphokines

(mature IL-10)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 273)
D.C. 1987, pp. 221-225; and Thompson-Snipes et al (1991) J. Exp. Med. 173, 507-510.

inflammatory disorders immunologic disorders, cancer

Human Interleukin-1 beta
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

precursor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R42447
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP569042
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P01584/IL1B_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-1 beta

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 274)
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334.

inflammatory disorders immunologic disorders, cancer

Human interleukin-6
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R49041 WO9403492
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P05231/IL6_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-6
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 270)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

inflammatory disorders immunologic disorders, cancer

Mutant Interleukin 6
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

S176R GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R54990
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9411402
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

S176R variant of wildtype
can be determined using assays known in the art: Matthews et al., in Lymphokines

IL-6 which has the

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

sequence:
D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

P05231/IL6_HUMAN
inflammatory disorders immunologic disorders, cancer

Interleukin-6

(SEQ ID NO: 270)

Interleukin 6 GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R55256
monocytes, and macrophages. Known functions include stimulating proliferation of

JP06145063
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P05231/IL6_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-6
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 270)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

inflammatory disorders immunologic disorders, cancer

Interleukin 8 (IL-8)
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R53932
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

JP06100595
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

GenBank: AAA59159.1
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 275)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Holmes et al (1991) Science 253, 1278-80. Soluble IL-8

receptor polypeptides may be useful for inhibiting interleukin activities.

Human interleukin-7
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R59919
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

U.S. Pat. No. 5,328,988
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P13232/IL7_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-7

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and Park et al (1990) J. Exp. Med. 171, 1073-79.

(SEQ ID NO: 276)
inflammatory disorders immunologic disorders, cancer

IL-3 containing fusion
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein. GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R79342 and
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

R79344 WO9521254
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Fusions of wildtype IL-3
can be determined using assays known in the art: Matthews et al., in Lymphokines

which has the sequence:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

P08700|IL3_HUMAN
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334.

Interleukin-3
inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 449)

IL-3 mutant proteins
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R79254, R79255, R79256,
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

R79257, R79258, R79259,
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

R79260, R79261, R79262,
can be determined using assays known in the art: Matthews et al., in Lymphokines

R79263, R79264, R79265,

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

R79266, R79267, R79268,
D.C. 1987, pp. 221-225; and Giri et al (1994) EMBO J. 13 2822-2830. inflammatory

R79269, R79270, R79271,
disorders immunologic disorders, cancer

R79272, R79273, R79274,

R79275, R79276, R79277,

R79278, R79279, R79280,

R79281, R79282, R79283,

R79284, and R79285

ZA9402636

Variants of wildtype IL-3

which has the sequence:

P08700|IL3_HUMAN

Interleukin-3

(SEQ ID NO: 449)

IL-12 p40 subunit.
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R63018 AU9466072
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P2946/|IL12B_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-12 subunit beta
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 277)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

AGF GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R64240 WO9429344
monocytes, and macrophages. Known functions include stimulating proliferation of

Q8NI99/ANGL6_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Angiopoietin-related
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

protein 6
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 278)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Human interlaukin-12 40 kD
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

subunit GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R79187
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9519786
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P2946/|IL12B_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-12 subunit beta

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 277)
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. inflammatory

disorders immunologic disorders, cancer

Human interleukin-15
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor from clone P1
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

R90843 WO9530695
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q13261|I15RA_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-15 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225; and Giri et al (1994) EMBO J. 13 2822-2830. Soluble IL-8

Isoform 1)
receptor polypeptides may be useful for inhibiting interleukin activities. Obesity.

(SEQ ID NO: 453)
Metabolic Disease. Diabetes. Enhancing secretion and stability of Interleukin 15

Human interleukin-7
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R92796 WO9604306
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P13232/IL7_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-7
can be determined using assays known in the art: Matthews et al., in Lymphokines

(isoform 1)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 276)
D.C. 1987, pp. 221-225; and Park et al (1990) J. Exp. Med. 171, 1073-79.

inflammatory disorders immunologic disorders, cancer

interleukin-9 GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R92797
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9604306
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P15248/IL9_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-9
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 279)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. inflammatory

disorders immunologic disorders, cancer

interleukin-3 GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R92801
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9604306
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P08700|IL3_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-3
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 280)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334.

inflammatory disorders immunologic disorders, cancer

Human interleukin-5
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R92802 WO9604306
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P05113/IL5_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-5
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 281)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334.

inflammatory disorders immunologic disorders, cancer

Recombinant interleukin-
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

16 GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W33373 DE19617202
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q14005/IL16_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Pro-interleukin-16
can be determined using assays known in the art: Matthews et al., in Lymphokines

(isoform 1)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 282)
D.C. 1987, pp. 221-225; and Lim et al (1996) J. Immunol. 156, 2566-70. inflammatory

disorders immunologic disorders, cancer

Human IL-16 protein
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W33234 DE19617202
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q14005/IL16_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Pro-interleukin-16
can be determined using assays known in the art: Matthews et al., in Lymphokines

(isoform 1)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 282)
D.C. 1987, pp. 221-225; and Lim et al (1996) J. Immunol. 156, 2566-70. inflammatory

disorders immunologic disorders, cancer

Thrl 17 human interleukin
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

9 GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W27521 WO9708321
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P15248|IL9_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-9
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 387)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Metl 17 human interleukin
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

9 GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W27522 WO9708321
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

(SEQ ID NO: 388)
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. inflammatory

disorders immunologic disorders, cancer

Human intracellular IL-1
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor antagonist.
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W77158 EP864585 (e.g.
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NOs: 12 to 19, or
can be determined using assays known in the art: Matthews et al., in Lymphokines

22 to 25 of this

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

publication).
D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

Human interleukin-18
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein (IL-18) GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W77158
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP864585
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q14116/IL18_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-18

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and USHIO et al (1996) J. Immunol. 156, 4274-79.

(SEQ ID NO: 283)
inflammatory disorders immunologic disorders, cancer

Human interleukin-18
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W77077 EP861663
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q14116/IL18_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-18
can be determined using assays known in the art: Matthews et al., in Lymphokines

(isoform 1)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 283)
D.C. 1987, pp. 221-225; and USHIO et al (1996) J. Immunol. 156, 4274-79.

inflammatory disorders immunologic disorders, cancer

Human interleukin 18
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

derivatives GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accessions W77083,
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W77084, W77085,
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

W77086, W77087,
can be determined using assays known in the art: Matthews et al., in Lymphokines

W77088, and W77089

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

EP861663
D.C. 1987, pp. 221-225; and Ushio et al (1996) J. Immunol, 156, 4274-79.

Variants of wildtype IL18
inflammatory disorders immunologic disorders, cancer

which is provided as:

Q14116/IL18_HUMAN

Interleukin-18

(isoform 1)

(SEQ ID NO: 283)

Interleukin-9 (IL-9) mature
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein (Thr117 version).
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W68158 WO9827997
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

FIG. 2 of WO9827997
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 389)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. inflammatory

disorders immunologic disorders, cancer

IL-9 mature protein variant
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

(Met117 version) GenSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W68157
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9827997
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

FIG. 3 of WO9827997
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 390)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. inflammatory

disorders immunologic disorders, cancer

Human IL-9 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein variant #3.
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W64058 WO9824904
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Wildtype IL-9R is provided
can be determined using assays known in the art: Matthews et al., in Lymphokines

as:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Q01113/IL9R_HUMAN
D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. inflammatory

Interleukin-9 receptor
disorders immunologic disorders, cancer

(isoform 1)

(SEQ ID NO: 303)

Human IL-9 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein variant fragment
monocytes, and macrophages. Known functions include stimulating proliferation of

GenSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W64060 WO9824904
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Wildtype IL-9R is provided
can be determined using assays known in the art: Matthews et al., in Lymphokines

as:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Q01113/IL9R_HUMAN
D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. Soluble IL-9

Interleukin-9 receptor
receptor polypeptides may be useful for inhibiting interleukin activities.

(isoform 1)

(SEQ ID NO: 303)

Human IL-9 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein variant #3.
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W64061 WO9824904
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Wildtype IL-9R is provided
can be determined using assays known in the art: Matthews et al., in Lymphokines

as:

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Q01113/IL9R_HUMAN
D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. Soluble IL-9

Interleukin-9 receptor
receptor polypeptides may be useful for inhibiting interleukin activities.

(isoform 1)

(SEQ ID NO: 303)

Human Interleukin-12 p40
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W51311
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9817689
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P2946/|IL12B_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-12 subunit beta

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 277)
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. inflammatory

disorders immunologic disorders, cancer

Human Interleukin-12 p35
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W51312
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9817689
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P29459/IL12A_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-12 subunit

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

alpha
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. inflammatory

(SEQ ID NO: 284)
disorders immunologic disorders, cancer

Human protein with IL-16
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

activity GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W63753
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

DE19649233-
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Lim et al (1996) J. Immunol. 156, 2566-70. inflammatory

disorders immunologic disorders, cancer

Human protein with IL-16
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

activity GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W59425
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

DE19649233-
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Lim et al (1996) J. Immunol. 156, 2566-70. inflammatory

disorders immunologic disorders, cancer

Human interleukin-15
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

W53878
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

U.S. Pat. No. 5,747,024
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P40933/IL15_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-15

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform IL15-S48AA)
D.C. 1987, pp. 221-225; and Giri et al (1994) EMBO J. 13 2822-2830. inflammatory

(SEQ ID NO: 285)
disorders immunologic disorders, cancer. Obesity. Metabolic Disease. Diabetes.

Human wild-type
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

interleukin-4 (hIL-4)
monocytes, and macrophages. Known functions include stimulating proliferation of

protein GeneSeq
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Accession W52149
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9747744
can be determined using assays known in the art: Matthews et al., in Lymphokines

P05112/IL4_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-4
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

(isoform 1)
287-295. inflammatory disorders immunologic disorders, cancer

(SEQ ID NO: 286)

interleukin-4 muteins
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accessions
monocytes, and macrophages. Known functions include stimulating proliferation of

W52150, W52151,
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W52153, W52154,
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

W52155, W52156,
can be determined using assays known in the art: Matthews et al., in Lymphokines

W52157, W52158,

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

W52159, W52160,
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

W52161, W52162,
287-295. inflammatory disorders immunologic disorders, cancer

W52163, W52164,

W52165, W52166, and

W52167 WO9747744

Variants of wildtype IL-4

which has the sequence:

P05112/IL4_HUMAN

Interleukin-4

(isoform 1)

(SEQ ID NO: 268)

Human interleukin 1 delta
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

Y28408 WO9935268
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 4 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9935268
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 287)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

Human interleukin-1
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor antagonist beta
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Y24395 WO9935268
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20.

inflammatory disorders immunologic disorders, cancer

Human EDIRF II protein
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

sequence GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession Y22199
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9932632
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 6 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9932632

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 391)
D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Human EDIRF I protein
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

sequence GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession Y22197
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9932632
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 2 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9932632

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 392)
D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

Human IL- 1RD10 protein
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

sequence GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession Y14131
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9919480
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 20 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9919480

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20. Soluble

IL-1RD10 receptor polypeptides may be useful for inhibiting interleukin activites.

Human IL- 1RD9
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

Y14122 WO9919480
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NOS: 6, 8, 10 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9919480
can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20. Soluble

IL-1RD10 receptor polypeptides may be useful for inhibiting interleukin activites.

Human DNAX interleukin-
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

40 GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

Y09196 WO9919491
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

SEQ ID NO: 2 or 4 of
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9919491
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 454)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

(DIL-40) alternative
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

sequence GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession Y09197
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9919491
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 4 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9919491

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 455)
D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

IL-11 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R50176 WO9405318
monocytes, and macrophages. Known functions include stimulating proliferation of

P2080/|IL11_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-11
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(isoform 1)
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 288)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Lu et al (1994) J immunol. Methods 173, 19.

inflammatory disorders immunologic disorders, cancer

Human adipogenesis
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

inhibitory factor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R43260
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP566410
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(also known as IL-11)
can be determined using assays known in the art: Matthews et al., in Lymphokines

P2080/|IL11_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-11
D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

(isoform 1)

(SEQ ID NO: 288)

IL-11 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

W02202 JP08127539
monocytes, and macrophages. Known functions include stimulating proliferation of

P2080/|IL11_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-11
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(isoform 1)
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 288)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Lu et al (1994) J immunol. Methods 173, 19.

inflammatory disorders immunologic disorders, cancer

IL-14 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R55800 WO9416074
monocytes, and macrophages. Known functions include stimulating proliferation of

P40222/TXLNA_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Alpha-taxilin
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(SEQ ID NO: 289)
can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Ambrus et al (1993) PNAS 90, 63330-34. inflammatory

disorders immunologic disorders, cancer

IL-17 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession B03807
monocytes, and macrophages. Known functions include stimulating proliferation of

U.S. Pat. No. 6,072,033
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q96F46/I17RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-17 receptor A
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 290)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yao et al (1995) J. Immunol. 155, 5483-86. Soluble IL-

17 receptor polypeptides may be useful for inhibiting interleukin activites.

IL-17 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

R76573 WO9518826
monocytes, and macrophages. Known functions include stimulating proliferation of

Q16552/IL17_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-17A
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(SEQ ID NO: 291)
can be determined using assays known in the art: Matthews et al., in Lymphokines

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yao et al (1995) J. Immunol. 155, 5483-86. inflammatory

disorders immunologic disorders, cancer

CTLA-8 GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession W13651
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9704097
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

(also known as IL-17)
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q16552/IL17_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-17A

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 291)
D.C. 1987, pp. 221-225. inflammatory disorders immunologic disorders, cancer

IL-19 GeneSeq Accession
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

W37935 WO9808870
monocytes, and macrophages. Known functions include stimulating proliferation of

Q9UHD0|IL19_HUMAN
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Interleukin-19
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

(isoform 1)
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 292)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Gallagher et al (2000) Genes Immun. 1,442-50.

inflammatory disorders immunologic disorders, cancer

IL-21 (TIF) GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession Y92879
monocytes, and macrophages. Known functions include stimulating proliferation of

WO0024758
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q9HBE4/IL21_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-21
can be determined using assays known in the art: Matthews et al., in Lymphokines

(isoform 1)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 293)
D.C. 1987, pp. 221-225; and Parrish-Novak et al (2000) Nature 408, 57-63.

inflammatory disorders immunologic disorders, cancer

IL-8 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R33420
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9306229
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

IL-8RA
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P25024/CXCR1_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

C-X-C chemokine receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

type 1
D.C. 1987, pp. 221-225; and Holmes et al (1991) Science 253, 1278-80. Soluble IL-8

(SEQ ID NO: 294)
receptor polypeptides may be useful for inhibiting interleukin activities.

IL-8RB

P25025/CXCR2_HUMAN

C-X-C chemokine receptor

type 2

(SEQ ID NO: 295)

Human type II interleukin-
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

1 receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R85480
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

U.S. Pat. No. 5,464,937
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P27930/IL1R2_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-1 receptor type 2

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 296)
D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20. Soluble

type II interleukin-1 receptor polypeptides may be useful for inhibiting interleukin

activities.

Human interleukin-12
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R69632
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP638644
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

IL-12 receptor B1
can be determined using assays known in the art: Matthews et al., in Lymphokines

P42701|I12R1_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-12 receptor
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. Soluble IL-12

subunit beta-1
receptor polypeptides may be useful for inhibiting interleukin activities.

(isoform 1)

(SEQ ID NO: 393)

IL-12 receptor B2

Q99665|I12R2_HUMAN

Interleukin-12 receptor

subunit beta-2

(isoform 1)

(SEQ ID NO: 394)

Interleukin 8 receptor B
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R80758
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

U.S. Pat. No. 5,440,021
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

IL-8RB
can be determined using assays known in the art: Matthews et al., in Lymphokines

P25025/CXCR2_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

C-X-C chemokine receptor
D.C. 1987, pp. 221-225; and Holmes et al (1991) Science 253, 1278-80. Soluble IL-8

type 2
receptor B polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 295)

Human IL-8 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein hIL8RA GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession B09989
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

JP08103276
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

IL-8RA
can be determined using assays known in the art: Matthews et al., in Lymphokines

P25024/CXCR1_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

C-X-C chemokine receptor
D.C. 1987, pp. 221-225; and Holmes et al (1991) Science 253, 1278-80. Soluble IL-8

type 1
receptor A polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 294)

Human IL-8 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein hIL8R GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession B09990
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

JP08103276
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

IL-8RA
can be determined using assays known in the art: Matthews et al., in Lymphokines

P25024/CXCR1_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

C-X-C chemokine receptor
D.C. 1987, pp. 221-225; and Holmes et al (1991) Science 253, 1278-80. Soluble IL-8

type 1
receptor polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 294)

IL-8RB

P25025/CXCR2_HUMAN

C-X-C chemokine receptor

type 2

(SEQ ID NO: 295)

Interleukin-2 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

associated protein p43
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

R97569 WO9621732-
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 2 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9621732

and Interferons: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 395)
D.C. 1987, pp. 221-225; and Gillis et al (1978) J. Immunol. 120, 2027. Soluble IL-2

receptor polypeptides may be useful for inhibiting interleukin activities.

Human interleukin-17
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W04185
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9629408
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q96F46/I17RA_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-17 receptor A

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 290)
D.C. 1987, pp. 221-225; and Yao et al (1995) J. Immunol. 155, 5483-86. Soluble IL-

17 receptor polypeptides may be useful for inhibiting interleukin activities.

Human interleukin-11
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R99090
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9619574
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q14626/I11RA_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-11 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225; and Lu et al (1994) J immunol. Methods 173, 19. Soluble IL-

(SEQ ID NO: 297)
11 receptor polypeptides may be useful for inhibiting interleukin activities.

Human interleukin-1
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor accessory protein
monocytes, and macrophages. Known functions include stimulating proliferation of

GeneSeq Accession
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W01911 WO9623067
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Human IL1R Acp
can be determined using assays known in the art: Matthews et al., in Lymphokines

SEQ ID NO: 3 of

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

WO9623067
D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20.

(SEQ ID NO: 396)
Inflammatory disorders immunologic disorders, cancer

Soluble Human IL1R Acp

SEQ ID NO: 9 of

WO9623067

(SEQ ID NO: 397)

AGF Protein GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R92749
monocytes, and macrophages. Known functions include stimulating proliferation of

U.S. Pat. No. 5,488,032
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q8NI99/ANGL6_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Angiopoietin-related
can be determined using assays known in the art: Matthews et al., in Lymphokines

protein 6

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 278)
D.C. 1987, pp. 221-225. Inflammatory disorders immunologic disorders, cancer

Human interleukin-1 type-
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

3 receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R91064
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W09607739
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 2 and 4 of
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9607739

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 398 and SEQ
D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1, 14-20. Soluble

ID NO: 399, respectively)
IL-type-3 receptor polypeptides may be useful for inhibiting interleukin activities

Human interleukin-13 beta
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W24972
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9720926
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

SEQ ID NO: 2 from
can be determined using assays known in the art: Matthews et al., in Lymphokines

WO9720926

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(SEQ ID NO: 400)
D.C. 1987, pp. 221-225; and Boutelier et al (1995) J. Immunol. Methods 181, 29.

Soluble IL-13 beta receptor polypeptides may be useful for inhibiting interleukin

activities.

Human interleukin-13
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

alpha receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W24973
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

WO9720926
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

IL-13RA1
can be determined using assays known in the art: Matthews et al., in Lymphokines

P78552/I13R1_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-13 receptor
D.C. 1987, pp. 221-225; and Boutelier et al (1995) J. Immunol. Methods 181, 29.

subunit alpha-1
Soluble IL-13 alpha receptor polypeptides may be useful for inhibiting interleukin

(isoform 1)
activities.

(SEQ ID NO: 298)

IL-13RA2

Q14627/I13R2_HUMAN

Interleukin-13 receptor

subunit alpha-2

(SEQ ID NO: 299)

Human interleukin-4
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W13499
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

U.S. Pat. No. 5,599,905
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P24394/IL4RA_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-4 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225; and Siegel & Mostowski (1990) J Immunol Methods 132,

(isoform 1)
287-295. Soluble IL-4 receptor polypeptides may be useful for inhibiting interleukin

(SEQ ID NO: 300)
activities.

Human interleukin-12
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

beta-2 receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W12771
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP759466
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q9966/|I12R2_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-12 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit beta-2
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. Soluble IL-12

(isoform 1)
beta-2 receptor polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 301)

Human interleukin-12
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

beta-1 receptor. GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession W12772
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP759466
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P4270/|I12R1_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-12 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit beta-1
D.C. 1987, pp. 221-225; and Hori et al (1987), Blood 70, 1069-1078. Soluble IL-12

(isoform 1)
beta-1 receptor polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 302)

Human IL-9 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

protein GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accessions W64055,
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

W64056, and W64057
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

WO9824904
can be determined using assays known in the art: Matthews et al., in Lymphokines

Q01113/IL9R_HUMAN

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

Interleukin-9 receptor
D.C. 1987, pp. 221-225; and Yang et al (1989) Blood 74, 1880-84. Soluble IL-9

(isoform 1)
receptor polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 303)

IL-10 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession W41804
monocytes, and macrophages. Known functions include stimulating proliferation of

U.S. Pat. No. 5,716,804
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

IL-10RA
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Q13651/I10R1_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-10 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225; and Thompson-Snipes et al (1991) J. Exp. Med. 173, 507-510.

(SEQ ID NO: 304)
Soluble IL-10 receptor polypeptides may be useful for inhibiting interleukin

IL-10RB
activities.

Q0833/|I10R2_HUMAN

Interleukin-10 receptor

subunit beta

(SEQ ID NO: 305)

Human IL-6 receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

Y30938 JP11196867
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P08887/IL6RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-6 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

(SEQ ID NO: 306)
Soluble IL-6 receptor polypeptides may be useful for inhibiting interleukin activities.

II-17 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession Y97181
monocytes, and macrophages. Known functions include stimulating proliferation of

U.S. Pat. No. 6,096,305
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q96F46/I17RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-17 receptor A
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 290)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yao et al (1995) J. Immunol. 155, 5483-86. Soluble IL-

17 receptor polypeptides may be useful for inhibiting interleukin activities.

II-17 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession Y97131
monocytes, and macrophages. Known functions include stimulating proliferation of

U.S. Pat. No. 6,100,235
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q96F46/I17RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-17 receptor A
can be determined using assays known in the art: Matthews et al., in Lymphokines

(SEQ ID NO: 290)

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

D.C. 1987, pp. 221-225; and Yao et al (1995) J. Immunol. 155, 5483-86. Soluble IL-

17 receptor polypeptides may be useful for inhibiting interleukin activities.

Human interleukin-3
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession R25300
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

EP509826
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P26951/IL3RA_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-3 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334. Soluble

(isoform 1)
IL-3 receptor polypeptides may be useful for inhibiting interleukin activities.

(SEQ ID NO: 307)

Human GM- CSF receptor
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R10919 WO9102063
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

GM-CSF receptor A
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P15509/CSF2R_HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Granulocyte-macrophage

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

colony-stimulating factor
D.C. 1987, pp. 221-225. Soluble GM-CSF receptor polypeptides may be useful for

receptor subunit alpha
inhibiting interleukin activities.

(isoform 1)

(SEQ ID NO: 308)

GM-CSF receptor B

P32927/IL3RB_HUMAN

Cytokine receptor

common subunit beta

(isoform 1)

(SEQ ID NO: 309)

Human IL-5 receptor alpha
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

chain GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R25064 EP492214
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q01344/IL5RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-5 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334. Soluble

(SEQ ID NO: 310)
IL-5 receptor alpha polypeptides may be useful for inhibiting interleukin activities.

II-5 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession W82842
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9847923
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q01344/IL5RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-5 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; Kitamura et al (1989) J Cell Physiol. 140 323-334. Soluble

(SEQ ID NO: 310)
IL-5 receptor polypeptides may be useful for inhibiting interleukin activities.

II-6 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R37215
monocytes, and macrophages. Known functions include stimulating proliferation of

JP05091892
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P08887/IL6RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-6 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and Aarden et al (1987) Eur. J. Immunol 17, 1411-16.

(SEQ ID NO: 306)
Soluble IL-6 receptor polypeptides may be useful for inhibiting interleukin activities.

Human B cell stimulating
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

factor-2 receptor GeneSeq
monocytes, and macrophages. Known functions include stimulating proliferation of

Accession P90525
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

AU8928720
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

P08887/IL6RA HUMAN
can be determined using assays known in the art: Matthews et al., in Lymphokines

Interleukin-6 receptor

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

subunit alpha
D.C. 1987, pp. 221-225. Soluble B cell stimulating factor-2 receptor polypeptides

(isoform 1)
may be useful for inhibiting interleukin activities.

(SEQ ID NO: 306)

IL-7 receptor clone
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

GeneSeq Accession
monocytes, and macrophages. Known functions include stimulating proliferation of

R08330 EP403114
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

P1687/|IL7RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-7 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and Park et al (1990) J. Exp. Med. 171, 1073-79. Soluble IL-

(SEQ ID NO: 311)
7 receptor polypeptides may be useful for inhibiting interleukin activities.

EPO receptor; EPOR
EPO Receptor is involved in the proliferation and differentiation of erythroblasts. EPO

GeneSeq Accession
Receptor activity can be determined using assays known in the art, such as, J Biol

R06512 WO9008822
Chem 2001 Mar. 23; 276(12: 8995-9002; JAK2 protein tyrosine kinase activity: Blood

P19235/EPOR_HUMAN
1994 Sep. 1; 84(5): 1501-7 and Mol Cell Biol. 1994 October; 14(10: 6506-14. Inflammatory

Erythropoietin receptor
disorders, immunologic disorders, cancer, erythroblast proliferation and differentiation

(isoform EPOR-F)

(SEQ ID NO: 312)

IL-15 receptor GeneSeq
Interleukins are a group of multifunctional cytokines synthesized by lymphocytes,

Accession R90843
monocytes, and macrophages. Known functions include stimulating proliferation of

WO9530695
immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis

Q1326/|I15RA_HUMAN
of neutrophils and T lymphocytes, and/or inhibition of interferons. Interleukin activity

Interleukin-15 receptor
can be determined using assays known in the art: Matthews et al., in Lymphokines

subunit alpha

and Interferens: A Practical Approach, Clemens et al., eds, IRL Press, Washington,

(isoform 1)
D.C. 1987, pp. 221-225; and Giri et al (1994) EMBO J. 13 2822-2830. Soluble IL-15

(SEQ ID NO: 313)
receptor polypeptides may be useful for inhibiting interleukin activities. Obesity.

Metabolic Disease. Diabetes. Enhancing secretion and stability of Interleukin 15

CD137; 4-1BB Receptor
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Protein GeneSeq
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

Accession R70977
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

WO9507984
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Q07011/TNR9_HUMAN
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble 4-1BB

Tumor necrosis factor
receptor polypeptides may be useful for inhibiting apoptosis, NF-kB activation,

receptor superfamily
and/or co-stimulation of immune cells such as B and T cells.

member 9

(SEQ ID NO: 314)

BCMA GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession Y71979
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO0068378
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Q02223/TNR17_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble BCMA

receptor superfamily
receptor polypeptides may be useful for inhibiting apoptosis, NF-kB activation,

member 17
and/or co-stimulation of immune cells such as B and T cells.

(isoform 1)

(SEQ ID NO: 315)

CD27 GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

R20814 WO9201049
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P26842/CD27_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

CD27 antigen
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

(SEQ ID NO: 316)
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble CD27

polypeptides may be useful for inhibiting apoptosis, NF-kB activation, and/or

co-stimulation of immune cells such as B and T cells.

CD30 GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

R35478 DE4200043
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P28908/TNR8_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

receptor superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble CD30

member 8
polypeptides may be useful for inhibiting apoptosis, NF-kB activation, and/or

(isoform 1)
co-stimulation of immune cells such as B and T cells.

(SEQ ID NO: 317)

CD40 GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Y33499 WO9945944
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P25942/TNR5_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

receptor superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble CD40

member 5
polypeptides may be useful for inhibiting apoptosis, NF-kB activation, and/or

(isoform 1)
co-stimulation of immune cells such as B and T cells.

(SEQ ID NO: 318)

EDAR Genbank
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession AAD50077
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

Q9UNE0|EDAR_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

receptor superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Immune Disorders,

member EDAR
Lymphomas, X-linked hypohidrotic ectodermal dysplasia

(isoform 1)

(SEQ ID NO: 319)

OX40; ACT-4 GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R74737
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9512673
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P43489/TNR4_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Immune Disorders,

receptor superfamily
Lymphomas, T cell disorders

member 4

(SEQ ID NO: 320)

TACI GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

W75783 WO9839361
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

O14836/TR13B_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

receptor superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble TACI

member 13B
receptor polypeptides may be useful for inhibiting apoptosis, NF-kB activation,

(isoform 1)
and/or co-stimulation of immune cells such as B and T cells.

(SEQ ID NO: 321)

TNF-R GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R10986
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

AU9058976
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P19438/TNR1A_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble TNF-R

receptor superfamily
receptor polypeptides may be useful for inhibiting apoptosis, NF-kB activation,

member 1A
and/or co-stimulation of immune cells such as B and T cells.

(isoform 1)

(SEQ ID NO: 322)

TNF-RII; TNF p75
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

receptor; Death Receptor
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

GeneSeq Accession
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

R11141 EP418014
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

P20333/TNR1B_HUMAN
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble TNFR-II

Tumor necrosis factor
receptor polypeptides may be useful for inhibiting apoptosis, NF-kB activation,

receptor superfamily
and/or co-stimulation of immune cells such as B and T cells.

member 1B

(isoform 1)

(SEQ ID NO: 323)

hAPO-4; TROY GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession W93581
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9911791
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Q9N568/TNR19_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Immune Disorders,

receptor superfamily
Cancers

member 19

(isoform 1)

(SEQ ID NO: 324)

TNF-alpha precursor
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

GeneSeq Accession
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P60074 EP205038
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

disorders immunologic disorders, cancer

Human TNF-alpha
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

GeneSeq Accession
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

R62463 EP619372
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P01375/TNFA_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(SEQ ID NO: 325)
disorders immunologic disorders, cancer

Human TNF-alpha
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

GeneSeq Accession
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

R42679 EP563714
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P01375/TNFA_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(SEQ ID NO: 325)
disorders immunologic disorders, cancer

Human TNF-beta (LT-
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

alpha) GeneSeq
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

Accession B37799
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

WO0064479
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

P01374/TNFB_HUMAN
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

Lymphotoxin-alpha
disorders immunologic disorders, cancer

(SEQ ID NO: 326)

LT-alpha GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession P70107
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

EP250000
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P01374/TNFB_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Lymphotoxin-alpha
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(SEQ ID NO: 326)
disorders immunologic disorders, cancer

LT-beta GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R56869
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9413808
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Q06643/TNFC_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Lymphotoxin-beta
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(isoform 1)
disorders immunologic disorders, cancer

(SEQ ID NO: 327)

OPGL GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession W83195
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9846751
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

O14788/TNF11_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

ligand superfamily
disorders immunologic disorders, cancer, loss of bone mass

member 11

(isoform 1)

(SEQ ID NO: 328)

FasL GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

W98071 WO9903999
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P48023/TNFL6_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

ligand superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

member 6
disorders immunologic disorders, cancer

(isoform 1)

(SEQ ID NO: 329)

FasL GeneSeq Accession
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

W95041 WO9903998
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

P48023/TNFL6_HUMAN
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

Tumor necrosis factor
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

ligand superfamily
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

member 6
disorders immunologic disorders, cancer

(isoform 1)

(SEQ ID NO: 329)

CD27L GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R50121
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9405691
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P32970/CD70_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

CD70 antigen
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(isoform 1)
disorders immunologic disorders, cancer

(SEQ ID NO: 330)

CD30 ligand GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R45007
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9324135
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P32971/TNFL8_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

ligand superfamily
disorders immunologic disorders, cancer

member 8

(SEQ ID NO: 331)

CD40L GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R85486
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9529935
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P29965/CD40L_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

CD40 ligand
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

(SEQ ID NO: 332)
disorders immunologic disorders, cancer

4-1BB ligand GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession W26657
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

U.S. Pat. No. 5,674,704
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P41273/TNFL9_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

ligand superfamily
disorders immunologic disorders, cancer

member 9

(SEQ ID NO: 333)

FAS Ligand Inhibitory
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Protein (DcR3) GeneSeq
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

Accession B19335
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

WO0058465
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

O95407/TNF6B_HUMAN
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Soluble DcR3

Tumor necrosis factor
polypeptides may be useful for inhibiting apoptosis, NF-kB activation, and/or

receptor superfamily
co-stimulation of immune cells such as B and T cells.

member 6B

(SEQ ID NO: 334)

OX40L GeneSeq
Activities associated with apoptosis, NF-kB activation, and co-stimulation of immune

Accession R79903
cells such as T and B cells. Apoptosis activity, NF-kB activation, and B and T cell

WO9521915
co-stimulation can be determined using assays known in the art: Moore et al., 1999,

P23510/TNFL4_HUMAN
Science, 285(5425): 260-3; Song H Y et al., 1997 Proc Natl Acad Sci USA 94(18):

Tumor necrosis factor
9792-6; Epsevik and Nissen-Meyer, 1986, J. Immunol. Methods. Inflammatory

ligand superfamily
disorders immunologic disorders, cancer

member 4

(isoform 1)

(SEQ ID NO: 335)

Protease inhibitor peptides
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

GeneSeq Accessions
the art: HIV protease assays: EP0387231. One can modify the assay to look for

R12435, R12436, R12437,
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

R12438, R12439, R12440,
inflammatory disorders, immunologic disorders, cancer, viral infections

and R1244 WO9106561

Retroviral protease
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

inhibitors GeneSeq
the art: HIV protease assays: EP0387231. One can modify the assay to look for

Accessions R06660,
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

R06661, R06662, R06663,
inflammatory disorders, immunologic disorders, cancer, viral infections

R06664, R06665, R06666,

R06667, R06668, R06669,

R06670, R06671, R06672,

R06673, R06674, R06675,

and R06676 EP387231

HIV protease inhibiting
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

peptides GeneSeq
the art: HIV protease assays: EP0387231. One can modify the assay to look for

Accessions R59293,
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

R59294, R59295, R59296,
inflammatory disorders, immunologic disorders, cancer, viral infections

R59297, R59298, R59299,

R592300, R59301,

R59302, R59301, R59302,

R59303, R59304, R59305,

R59306, R59307, R59308,

R59309, R59310, R59311,

R59312, R59313, R59314,

R59315, R59316, R59317

R59318, R59319, R59320,

R59321, R59322, R59323,

R59324, R59325, R59326,

R59327, R59328, R59329,

R59330, R59331, R59332,

R59333, R59334, R59335,

R59336, R59337, R59338,

R59339, R59340, R59341,

R59342, R59343, R59344,

R59345, R59346, R59347,

R59348, R59349, and

R59350 WO9301828

HIV-1 protease inhibitors
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

GeneSeq Accessions
the art: HIV protease assays: EP0387231. One can modify the assay to look for

R86326, R86327, R86328,
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

R86329, R86330, R86331,
inflammatory disorders, immunologic disorders, cancer, viral infections

R86332, R86333, R86334,

R86335, R86336, R86337,

R86338, R86339, R86340,

R86341, R86342, R86343,

R86344, R86345, R86346,

R86347, R86348, R86349,

R86350, R86351, R86352,

R86353, R86354, R86355,

R86356, R86357, R86358,

R86359, R86360, R86361,

R86362, R86363, R86364,

R86365, R86366, R86367,

R86368, R86369, R86370,

and R86371 DE4412174

HIV Inhibitor Peptide
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

GeneSeq Accession
the art: HIV protease assays: EP0387231. One can modify the assay to look for

Y89687 WO9959615
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

inflammatory disorders, immunologic disorders, cancer, viral infections

HIV Inhibitor Peptide
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

GenSeq Accession
the art: HIV protease assays: EP0387231. One can modify the assay to look for

Y31955 WO9948513
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

inflammatory disorders, immunologic disorders, cancer, viral infections

HIV Inhibitor Peptide
Peptides that inhibit the function/binding of HIV HIV protease activities are known in

Science 291, 884 (2001);
the art: HIV protease assays: EP0387231. One can modify the assay to look for

Published online 12 Jan.
inhibition using any of the disclosed protease inhibitor polypeptides. HIV,

2001; 10.1126/science.1057453
inflammatory disorders, immunologic disorders, cancer, viral infections

Human monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemoattractant factor
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

hMCP-3 GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession R73915
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9509232
chemokines exert their effects by acting on a family of seven transmembrane G-

P80098/CCL7_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 7
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 336)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, particularly useful for treating

bacterial and/or viral menigitis

Human monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemoattractant factor
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

hMCP-1 GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession R73914
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9509232
chemokines exert their effects by acting on a family of seven transmembrane G-

P13500/CCL2_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 2
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 337)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, particularly useful for treating

bacterial and/or viral menigitis

Human gro-beta
Chemokines are a family of related small, secreted proteins involved in biological

chemokine GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accessions R66699 and
Members of this family are involved in a similarly diverse range of pathologies

W17671 WO9429341
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P19875/CXCL2_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 2
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 338)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, inflammatory disorders, blood-

related disorders, stem cell transplantation, cancer

Human gro-gamma
Chemokines are a family of related small, secreted proteins involved in biological

chemokine GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accessions R66700 and
Members of this family are involved in a similarly diverse range of pathologies

W17672 WO9429341
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P19876/CXCL3_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 3
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 339)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, inflammatory disorders, blood-

related disorders, stem cell transplantation, cancer

Human gro-alpha
Chemokines are a family of related small, secreted proteins involved in biological

chemokine GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accessions R66698 and
Members of this family are involved in a similarly diverse range of pathologies

W18024 WO9429341
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P09341/GROA_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Growth-regulated alpha
protein coupled receptors. Over 40 human chemokines have been described, which

protein
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 340)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, inflammatory disorders, blood-

related disorders, stem cell transplantation, cancer

Human eosinophil-
Chemokines are a family of related small, secreted proteins involved in biological

expressed chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(EEC) GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W05186
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9632481
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 2 of
protein coupled receptors. Over 40 human chemokines have been described, which

WO9632481
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 401)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, particularly treatment of

eosinophilia, inflammation, allergies, asthma, leukaemia and lymphoma

Chemokine-like protein
Chemokines are a family of related small, secreted proteins involved in biological

PF4-414 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R92318 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99809 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 3C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 402)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and blood-related disorders, particularly

myelosuppression

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M3 GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

R99812 WO9613587
Members of this family are involved in a similarly diverse range of pathologies

including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression

Human interleukin-8 (IL-8)
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

R99814 WO9613587
Members of this family are involved in a similarly diverse range of pathologies

P10145/IL8_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Interleukin-8
chemokines exert their effects by acting on a family of seven transmembrane G-

(isoform 1)
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 341)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M1 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99815 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99803 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4B of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 403)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M8 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99816 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99805 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 404)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M8 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99817 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99806 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 404)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression.

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M8 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99818 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99804 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 404)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Cancer and blood-related disorders, particularly myelosuppression.

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M8 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99819 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R99807 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 404)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and blood-related disorders, particularly

myelosuppression.

Chemokine-like protein IL-
Chemokines are a family of related small, secreted proteins involved in biological

8M8 Full-Length and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Mature GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions R99822 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

R9807 WO9613587
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 4C of WO9613587
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 404)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and blood-related disorders, particularly

myelosuppression.

Human foetal spleen expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemo-kine,
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

FSEC GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession R98499
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9622374
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 2 of
protein coupled receptors. Over 40 human chemokines have been described, which

WO9622374
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 405)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders

Liver expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine-1(LVEC-1)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

R95689 WO9616979
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9616979
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 406)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammation of the liver

Liver expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine-2(LVEC-2)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

R95690 WO9616979
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 4 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9616979
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 407)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammation of the liver

Pituitary expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine (PGEC)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

R95691 WO9616979
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 6 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9616979
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 408)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammation, particularly of the liver

Adenoid-expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine (ADEC)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

R97664 WO9617868
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9617868
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 409)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammation, angiogenesis, tumorigenesis,

musculoskeletal disorders

Human chemokine CC-2
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W38170 WO9741230
Members of this family are involved in a similarly diverse range of pathologies

Q16663/CCL15_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 15
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 342)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cell migration, proliferation, and

differentiation disorders

Human chemokine HCC-1
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W38171 WO9741230
Members of this family are involved in a similarly diverse range of pathologies

Q16627/CCL14_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 14
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 343)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cell migration, proliferation, and

differentiation disorders

Human chemokine CC-3
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W38172 WO9741230
Members of this family are involved in a similarly diverse range of pathologies

Q16627/CCL14_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 14
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 343)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cell migration, proliferation, and

differentiation

Novel betachemokine
Chemokines are a family of related small, secreted proteins involved in biological

designated PTEC
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W27271 WO9739126
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9739126
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 410)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, vascular disorders, cancer

Human CX3C 111 amino
Chemokines are a family of related small, secreted proteins involved in biological

acid chemokine GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W23344
Members of this family are involved in a similarly diverse range of pathologies

WO9727299
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9727299
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 411)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, inflammatory diseases,

abnormal proliferation, regeneration, degeneration, and atrophy

Human CCF18 chemokine
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W25942 WO9721812
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 4 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9721812
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 412)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Abnormal physiology and development disorders,

can also be used as an antiviral agent

Human beta- chemokine
Chemokines are a family of related small, secreted proteins involved in biological

H1305 (MCP-2) GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W26655
Members of this family are involved in a similarly diverse range of pathologies

WO9725427
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P80075/CCL8_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 8
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 344)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Chemotaxis, blood-related disorders, viral

infection, HIV, wound healing, cancer

Human eosinocyte CC
Chemokines are a family of related small, secreted proteins involved in biological

type chemokine eotaxin
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W14990 WO9712914
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P51671/CCL11_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Eotaxin
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 245)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammatory and immune disorders

Human thymus and
Chemokines are a family of related small, secreted proteins involved in biological

activation regulated
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

cytokine (TARC) GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W14018
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9711969
chemokines exert their effects by acting on a family of seven transmembrane G-

Q92583/CCL17_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 17
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 261)
using assays known in the art: Methods in molecular Biology, 2000, vol. 138:

Chemokine Protocols, Edited by A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power

Humana Press Inc., Totowa, NJ Inflammatory and immune disorders

Human chemokine beta-8
Chemokines are a family of related small, secreted proteins involved in biological

short forms GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W16315
Members of this family are involved in a similarly diverse range of pathologies

WO9712041
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Wildtype chemokine beta-
chemokines exert their effects by acting on a family of seven transmembrane G-

8 provided as:
protein coupled receptors. Over 40 human chemokines have been described, which

P55773|CCL23_HUMAN
bind to ~17 receptors thus far identified. Chemokine activities can be determined

C-C motif chemokine 23
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 459)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing, immune disorders

Microphage derived
Chemokines are a family of related small, secreted proteins involved in biological

chemokine, MDC
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W20058 WO9640923
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

O00626/CCL22_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 22
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 345)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammatory diseases, wound healin, angiogenesis

Human chemokine ZSIG-
Chemokines are a family of related small, secreted proteins involved in biological

35 GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W30565 WO9844117
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 2 of WO
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9844117
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 413)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammatory and immune diseases

Primate CC chemokine
Chemokines are a family of related small, secreted proteins involved in biological

“ILINCK” GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accesssion W69990
Chemokine activities can be determined using assays known in the art: Methods in

WO98328658
Molecular Biology, 2000, vol. 138: Chemokine Protocols. Edited by: A. E. I.

SEQ ID NO: 4 from
Proudfoot, T. N. C. Wells, and C. A. Power. Humana Press Inc., Totowa, NJ.

WO9832858
Immune and inflammatory disorders, abnormal proliferation, regeneration,

(SEQ ID NO: 414)
generation and atrophy disorders

Primate CXC chemokine
Chemokines are a family of related small, secreted proteins involved in biological

“IBICK” GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W69989
Members of this family are involved in a similarly diverse range of pathologies

WO9832858
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 from
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9832858
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 415)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders, abnormal

proliferation, regeneration, generation and atrophy disorders

Human CC-type
Chemokines are a family of related small, secreted proteins involved in biological

chemokine protein
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

designated SLC
Members of this family are involved in a similarly diverse range of pathologies

(secondary lymphoid
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokine) GeneSeq
chemokines exert their effects by acting on a family of seven transmembrane G-

Accession W69163
protein coupled receptors. Over 40 human chemokines have been described, which

WO9831809
bind to ~17 receptors thus far identified. Chemokine activities can be determined

O00585/CCL21_HUMAN
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

C-C motif chemokine 21
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 346)
Humana Press Inc., Totowa, NJ. Immune, inflammatory, and infectious disorders,

cancer

Human CC chemokine
Chemokines are a family of related small, secreted proteins involved in biological

ELC protein GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W62542
Members of this family are involved in a similarly diverse range of pathologies

WO9826071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Q99731/CCL19_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 19
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 249)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and infectious diseases, particularly herpes

virus

Human DVic-1 C-C
Chemokines are a family of related small, secreted proteins involved in biological

chemokine GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W60649
Members of this family are involved in a similarly diverse range of pathologies

WO9823750
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9823750
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 416)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Abnormal proliferation, regeneration,

degeneration, and atrophy disorders, including cancer

Human C-C chemokine
Chemokines are a family of related small, secreted proteins involved in biological

DGWCC GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W60650
Members of this family are involved in a similarly diverse range of pathologies

WO9823750
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 6 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9823750
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 417)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cell proliferation disorders,

cancer

Human STCP-1 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W62783
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9824907
Members of this family are involved in a similarly diverse range of pathologies

O00626/CCL22_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 22
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 345)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, particularly T cell related

disorders, viral infection, and inflammation, especially joint

Exodus protein GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W61279
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9821330
Members of this family are involved in a similarly diverse range of pathologies

P78556/CCL20_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 20
chemokines exert their effects by acting on a family of seven transmembrane G-

(isoform 1)
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 248)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders, angiogenesis,

cancer, and proliferation disorders, particularly myeloproliferative diseases

Human Chr19kine protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Acession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W50887 WO9814581
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 10 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9814581
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 418)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and degenerative disorders

Human T cell mixed
Chemokines are a family of related small, secreted proteins involved in biological

lymphocyte reaction
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

expressed chemokine
Members of this family are involved in a similarly diverse range of pathologies

(TMEC) GeneSeq
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Accession W58703
chemokines exert their effects by acting on a family of seven transmembrane G-

U.S. Pat. No. 5,780,268
protein coupled receptors. Over 40 human chemokines have been described, which

SEQ ID NO: 2 of
bind to ~17 receptors thus far identified. Chemokine activities can be determined

U.S. Pat. No. 5,780,268
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 460)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune, inflammatory, and infectious disorders,

cancer

Human 6CKine protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W50885 W09814581
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 8 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9814581
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 419)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and degenerative disorders

human liver and activation
Chemokines are a family of related small, secreted proteins involved in biological

regulated chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(LARC) GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W57475
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9817800
chemokines exert their effects by acting on a family of seven transmembrane G-

P78556/CCL20_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 20
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(isoform 1)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 248)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune, inflammatory, and infectious disorders,

cancer

RANTES peptide
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W29538 WO9744462
Members of this family are involved in a similarly diverse range of pathologies

Wildtype Rantes provided
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

herien as
chemokines exert their effects by acting on a family of seven transmembrane G-

P13501/CCL5_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 5
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 241)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Infectious diseases, particularly HIV

RANTES 8-68 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W29529
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9744462
Members of this family are involved in a similarly diverse range of pathologies

Wildtype Rantes provided
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

herein as
chemokines exert their effects by acting on a family of seven transmembrane G-

P13501/CCL5_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 5
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 241)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Infectious diseases, particularly HIV

RANTES 9-68 GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession W29528
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9744462
Members of this family are involved in a similarly diverse range of pathologies

Wildtype Rantes provided
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

herien as
chemokines exert their effects by acting on a family of seven transmembrane G-

P13501/CCL5_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 5
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 241)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Infectious diseases, particularly HIV

Human chemokine protein
Chemokines are a family of related small, secreted proteins involved in biological

331D5 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W59433
Members of this family are involved in a similarly diverse range of pathologies

WO9811226
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 12 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9811226
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 420)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Abnormal proliferation, regeneration,

degeneration, or atrophy, including cancer

Human chemokine protein
Chemokines are a family of related small, secreted proteins involved in biological

61164 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W59430
Members of this family are involved in a similarly diverse range of pathologies

WO9811226
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 6 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9811226
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 421)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Abnormal proliferation, regeneration,

degeneration, or atrophy, including cancer

Chemokine MCP-4
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W56690 WO9809171
Members of this family are involved in a similarly diverse range of pathologies

Q99616/CCL13_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 13
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 347)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune, Inflammatory, and infectious diseases

Human stromal cell-
Chemokines are a family of related small, secreted proteins involved in biological

derived chemokine, SDF-1
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W50766 FR2751658
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P48061/SDF1_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Stromal cell-derived factor 1
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform beta)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 260)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. HIV infections

Thymus expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine (TECK)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W44397 WO9801557
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

O15444/CCL25_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 25
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 348)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

Human chemokine MIP-
Chemokines are a family of related small, secreted proteins involved in biological

3alpha GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W44398
Members of this family are involved in a similarly diverse range of pathologies

WO9801557
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P78556/CCL20_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 20
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform 1)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 248)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

Human chemokine MIP-
Chemokines are a family of related small, secreted proteins involved in biological

3beta GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W44399 WO9801557
Members of this family are involved in a similarly diverse range of pathologies

Q99731/CCL19_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 19
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 249)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

Human monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemotactic proprotein
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(MCPP) sequence
Members of this family are involved in a similarly diverse range of pathologies

GeneSeq Accession
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

W42072 WO9802459
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 1 of
protein coupled receptors. Over 40 human chemokines have been described, which

WO9802459
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 456)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, respiratory disorders, cancer

Macrophage- derived
Chemokines are a family of related small, secreted proteins involved in biological

chemokine (MDC)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accessions
Members of this family are involved in a similarly diverse range of pathologies

W40811 and Y24414
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,688,927/
chemokines exert their effects by acting on a family of seven transmembrane G-

U.S. Pat. No. 5,932,703
protein coupled receptors. Over 40 human chemokines have been described, which

O00626/CCL22_HUMAN
bind to ~17 receptors thus far identified. Chemokine activities can be determined

C-C motif chemokine 22
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 345)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune, and inflammatory disorders, cancer

Macrophage derived
Chemokines are a family of related small, secreted proteins involved in biological

chemokine analogue
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

MDC-eyfy GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession Y24416
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,932,703 (“eyfy”
chemokines exert their effects by acting on a family of seven transmembrane G-

disclosed as SEQ ID NO:
protein coupled receptors. Over 40 human chemokines have been described, which

546)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

Wildtype MDC is SEQ ID
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

NO: 2 of
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

U.S. Pat. No. 5,932,703
Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

(SEQ ID NO: 422)
Chemokines are a family of related small, secreted proteins involved in biological

Macrophage derived
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

chemokine analogue MDC
Members of this family are involved in a similarly diverse range of pathologies

(n + 1) GeneSeq
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Accession Y24413
chemokines exert their effects by acting on a family of seven transmembrane G-

U.S. Pat. No. 5,932,703
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

Macrophage derived
Chemokines are a family of related small, secreted proteins involved in biological

chemokine analogue
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

MDC-yl GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession Y24415
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,932,703
chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and inflammatory disorders

Human type CC
Chemokines are a family of related small, secreted proteins involved in biological

chemokine eotaxin 3
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

protein sequence
Members of this family are involved in a similarly diverse range of pathologies

GeneSeq Accession
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Y43178 JP11243960
chemokines exert their effects by acting on a family of seven transmembrane G-

Q9Y258/CCL26_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 26
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 349)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Allergic diseases and HIV infection

Human MCP-3 and human
Chemokines are a family of related small, secreted proteins involved in biological

Muc-1 core epitope (VNT)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

fusion protein GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Acession Y29893
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9946392
chemokines exert their effects by acting on a family of seven transmembrane G-

Wildtype MCP-3 has the
protein coupled receptors. Over 40 human chemokines have been described, which

sequence:
bind to ~17 receptors thus far identified. Chemokine activities can be determined

P80098/CCL7_HUMAN
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

C-C motif chemokine 7
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 336)
Humana Press Inc., Totowa, NJ. Cancer and immune disorders, particularly HIV

Wildtype Muc-1 has the
infection

sequence:

P15941|MUC1_HUMAN

Mucin-1

(isoform 1)

(SEQ ID NO: 461)

Human IP-10 and human
Chemokines are a family of related small, secreted proteins involved in biological

Muc-1 core epitope (VNT)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

fusion protein GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession Y29894
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9946392
chemokines exert their effects by acting on a family of seven transmembrane G-

Wildtype IP10 has the
protein coupled receptors. Over 40 human chemokines have been described, which

sequence:
bind to ~17 receptors thus far identified. Chemokine activities can be determined

P02778/CXL10_HUMAN
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

C-X-C motif chemokine 10
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 242)
Humana Press Inc., Totowa, NJ. Cancer and immune disorders, particularly HIV

Wildtype Muc-1 has the
infection

sequence:

P15941|MUC1_HUMAN

Mucin-1

(isoform 1)

(SEQ ID NO: 461)

Human IP-10 and HIV-1
Chemokines are a family of related small, secreted proteins involved in biological

gp 120 hyper- variable
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

region fusion protein
Members of this family are involved in a similarly diverse range of pathologies

GeneSeq Accession
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Y29897 W09946392
chemokines exert their effects by acting on a family of seven transmembrane G-

Wildtype IP10 has the
protein coupled receptors. Over 40 human chemokines have been described, which

sequence:
bind to ~17 receptors thus far identified. Chemokine activities can be determined

P02778/CXL10_HUMAN
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

C-X-C motif chemokine 10
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 242)
Humana Press Inc., Totowa, NJ. Cancer and immune disorders, particularly HIV

Wildtype gp120 has the
infection

sequence:

P03378|32-509

(cleaved product of gp160)

(SEQ ID NO: 462)

Human mammary
Chemokines are a family of related small, secreted proteins involved in biological

associated chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(MACK) protein Full-
Members of this family are involved in a similarly diverse range of pathologies

Length and Mature
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

GeneSeq Accessions
chemokines exert their effects by acting on a family of seven transmembrane G-

Y29092 and Y29093
protein coupled receptors. Over 40 human chemokines have been described, which

WO9936540
bind to ~17 receptors thus far identified. Chemokine activities can be determined

Full-length: SEQ ID NO: 1
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

of WO9936540
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 423)
Humana Press Inc., Totowa, NJ. Breast disease, including cancer

Mature Form: SEQ ID

NO: 2 of WO9936540

(SEQ ID NO: 424)

Tim-1 protein GeneSeq
Chemokines are a family of related small, secreted proteins involved in biological

Accession Y28290
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

WO9933990
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 2 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9933990
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 350)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammation due to stimuli such as heart attacks

and stroke, infection, physical trauma, UV or ionizing radiation, burns, frostbite or

corrosive chemicals

Human Lkn-1 Full-Length
Chemokines are a family of related small, secreted proteins involved in biological

and Mature protein
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accessions
Members of this family are involved in a similarly diverse range of pathologies

Y17280, Y17274, Y17281,
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

and Y17275 WO9928473
chemokines exert their effects by acting on a family of seven transmembrane G-

and WO9928472
protein coupled receptors. Over 40 human chemokines have been described, which

Q16663/CCL15_HUMAN
bind to ~17 receptors thus far identified. Chemokine activities can be determined

C-C motif chemokine 15
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 342)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. HIV infection and cancer, particularly leukemia

N-terminal modified
Chemokines are a family of related small, secreted proteins involved in biological

chemokine met-hSDF-1
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

alpha GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y05818 WO9920759
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 10 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9920759
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 425)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inhibit or stimulate angiogenesis, inhibit the binding

of HIV

N-terminal modified
Chemokines are a family of related small, secreted proteins involved in biological

chemokine met-hSDF-1
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

beta GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y05819 WO9920759
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 11 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9920759
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 426)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inhibit or stimulate angiogenesis, inhibit the binding

of HIV, antiinflammatory; immunosuppressant

N-terminal modified
Chemokines are a family of related small, secreted proteins involved in biological

chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GroHEK/hSDF-1alpha
Members of this family are involved in a similarly diverse range of pathologies

GeneSeq Accession
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Y05820 WO9920759
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 12 of
protein coupled receptors. Over 40 human chemokines have been described, which

WO9920759
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 427)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inhibit or stimulate angiogenesis, inhibit the binding

of HIV, antiinflammatory; immunosuppressant

N-terminal modified
Chemokines are a family of related small, secreted proteins involved in biological

chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GroHEK/hSDF-1beta.
Members of this family are involved in a similarly diverse range of pathologies

GeneSeq Accession
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Y05821 WO9920759
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 13 of
protein coupled receptors. Over 40 human chemokines have been described, which

WO9920759
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 428)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inhibit or stimulate angiogenesis, inhibit the binding

of HIV, antiinflammatory; immunosuppressant

Chemokine Eotaxin
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14230 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P51671/CCL11_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Eotaxin
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 245)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Increase or enhance an inflammatory response, an

immune response orhaematopoietic cell-associated activity; treat a vascular

indication; Cancer; enhance wound healing, to prevent or treat asthma, organ

transplant rejction, rheumatoid arthritis or allergy

Chemokine hMCP1a
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14225 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

Chemokine hMCP1b
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14226 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

Chemokine hSDF1b
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14228 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P48061/SDF1_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Stromal cell-derived factor 1
chemokines exert their effects by acting on a family of seven transmembrane G-

(isoform beta)
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 260)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

Chemokine hIL-8
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14229 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P10145/IL8_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Interleukin-8
chemokines exert their effects by acting on a family of seven transmembrane G-

(isoform 1)
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 341)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Immune disorders, Vascular disorders, Wound healing, cancer, prevent organ

transplant rejection, Increase or enhance an inflammatory response,

Chemokine hMCP1
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14222 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P13500/CCL2_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 2
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 337)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

Chemokine hMCP2
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14223 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P80075/CCL8_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 8
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 344)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

Chemokine hMCP3
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y14224 WO9912968
Members of this family are involved in a similarly diverse range of pathologies

P80098/CCL7_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 7
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 336)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, Vascular disorders, Wound

healing, cancer, prevent organ transplant rejection, Increase or enhance an

inflammatory response,

C-C chemokine, MCP2
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Y05300 EP905240
Members of this family are involved in a similarly diverse range of pathologies

P80075/CCL8_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 8
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 344)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammatory, Immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

Wild type monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemotactic protein 2
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y07233 EP906954
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P80075/CCL8_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 8
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 344)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Inflammatory, Immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

Truncated monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemotactic protein 2 (6-
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

76) GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y07234 EP906954
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

FIG. 1 of EP905241 and
chemokines exert their effects by acting on a family of seven transmembrane G-

EP906954
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 429)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Inflammatory, Immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

Truncated RANTES
Chemokines are a family of related small, secreted proteins involved in biological

protein (3-68) GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accessions Y07236 and
Members of this family are involved in a similarly diverse range of pathologies

Y07232 EP905241;
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

EP906954
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 1 of EP906954
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 430)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Inflammatory, immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

Wild type monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemotactic protein 2
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y07237 EP905241
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P80075/CCL8_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 8
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 344)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Inflammatory, immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

Truncated monocyte
Chemokines are a family of related small, secreted proteins involved in biological

chemotactic protein 2 (6-76)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y07238 EP905241
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

FIG. 1 of EP905241 and
chemokines exert their effects by acting on a family of seven transmembrane G-

EP906954
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 429)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Inflammatory, immune and infectious diseases;

pulmonary diseases and skin disorders; tumours, and angiogenesis-and

haematopoiesis-related diseases

A partial CXCR4B protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W97363 EP897980
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 2 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

EP897980
chemokines exert their effects by acting on a family of seven transmembrane G-

(sEQ ID NO: 431)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Soluble CXCR4B receptor polypeptides may be

useful for inhibiting chemokine activities and viral infection.

Interferon gamma-
Chemokines are a family of related small, secreted proteins involved in biological

inducible protein (IP-10)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96709
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P02778/CXL10_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 10
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 242)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

A monokine induced by
Chemokines are a family of related small, secreted proteins involved in biological

gamma-interferon (MIG)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96710
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

Q07325/CXCL9_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 9
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 351)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Interleukin-8 (IL-8) protein.
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W96711
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 5,871,723
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P10145/IL8_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Interleukin-8
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform 1)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 341)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ; and Holmes et al (1991) Science 253, 1278-80.

Angiogenesis, Cancer, Inflammatory and Immune disorders, Cardio-Vascular

disorders, Musco-skeletal disorders

Epithelial neutrophil
Chemokines are a family of related small, secreted proteins involved in biological

activating protein-78
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(ENA-78) GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W96712
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P42830/CXCL5_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 5
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 352)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Growth related oncogene-
Chemokines are a family of related small, secreted proteins involved in biological

alpha (GRO-alpha).
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96713
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P09341/GROA_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

Growth-regulated alpha
bind to ~17 receptors thus far identified. Chemokine activities can be determined

protein
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 340)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power,

Humana Press Inc., Totowa, NJ Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Growth related oncogene-
Chemokines are a family of related small, secreted proteins involved in biological

beta (GRO-beta).
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96714
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P19875/CXCL2_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 2
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 338)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Growth related oncogene-
Chemokines are a family of related small, secreted proteins involved in biological

gamma (GRO-gamma)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96715
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P19876/CXCL3_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 3
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 339)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

A platelet basic protein
Chemokines are a family of related small, secreted proteins involved in biological

(PBP) GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W96716
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 5,871,723
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P02775/CXCL7_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Platelet basic protein
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 353)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Connective tissue
Chemokines are a family of related small, secreted proteins involved in biological

activating protein-III
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(CTAP-III) GeneSeqAccession
Members of this family are involved in a similarly diverse range of pathologies

S96717
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 9 of
protein coupled receptors. Over 40 human chemokines have been described, which

U.S. Pat. No. 5,871,723
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 354)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Beta-thrombo-globulin
Chemokines are a family of related small, secreted proteins involved in biological

protein (beta-TG)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96718
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 10 of
protein coupled receptors. Over 40 human chemokines have been described, which

U.S. Pat. No. 5,871,723
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 355)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Neutrophil activating
Chemokines are a family of related small, secreted proteins involved in biological

peptide-2 (NAP-2)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96719
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

SEQ ID NO: 11 of
protein coupled receptors. Over 40 human chemokines have been described, which

U.S. Pat. No. 5,871,723
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 356)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Granulocyte chemotactic
Chemokines are a family of related small, secreted proteins involved in biological

protein-2 (GCP-2)
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W96720
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 5,871,723
chemokines exert their effects by acting on a family of seven transmembrane G-

P80162/CXCL6_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 6
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 357)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Angiogenesis, Cancer, Inflammatory and Immune

disorders, Cardio-Vascular disorders, Musco-skeletal disorders

Human chemokine MIG-
Chemokines are a family of related small, secreted proteins involved in biological

beta protein GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession W90124
Members of this family are involved in a similarly diverse range of pathologies

EP887409
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

(SEQ ID NO: 463)
chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, viral, parasitic, fungal or

bacterial infections, Cancer; autoimmune diseases or transplant rejection

Human ZCHEMO-8
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82716 WO9854326
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 2 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9854326
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 432)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

Human Act-2 protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82717 WO9854326
Members of this family are involved in a similarly diverse range of pathologies

P13236/CCL4_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 4
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 358)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

Human SISD protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Acession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82720 WO9854326
Members of this family are involved in a similarly diverse range of pathologies

P13501/CCL5_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 5
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 241)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

Human MI10 protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82721 WO9854326
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 37 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9854326
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 433)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

Human MI1A protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82722 W09854326
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 38 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9854326
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 434)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

Human CCC3 protein
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

W82723 WO9854326
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 39 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9854326
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 435)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune disorders, cancer, myelopoietic disorders,

autoimmune disorders and immunodeficiencies, Inflammatory and infectious

diseases, Vascular disorders, wound healing

A human L105 chemokine
Chemokines are a family of related small, secreted proteins involved in biological

designated huL105_3.
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W87588 WO9856818
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9856818
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 436)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing

A human L105 chemokine
Chemokines are a family of related small, secreted proteins involved in biological

designated huL105_7.
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

W87589 WO9856818
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 4 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9856818
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 437)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing

Human mature gro-alpha
Chemokines are a family of related small, secreted proteins involved in biological

polypeptide used to treat
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

sepsis GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W81498
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9848828
chemokines exert their effects by acting on a family of seven transmembrane G-

P09341/GROA_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

Growth-regulated alpha
bind to ~17 receptors thus far identified. Chemokine activities can be determined

protein
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 340)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Infectious diseases, sepsis

Human mature gro-
Chemokines are a family of related small, secreted proteins involved in biological

gamma polypeptide used
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

to treat sepsis GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession W81500
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO9848828
chemokines exert their effects by acting on a family of seven transmembrane G-

P19876/CXCL3_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-X-C motif chemokine 3
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 339)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Infectious diseases, sepsis

Human thymus expressed
Chemokines are a family of related small, secreted proteins involved in biological

chemokine TECK and
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

TECK variant GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accessions B19607 and
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

B19608 WO0053635
chemokines exert their effects by acting on a family of seven transmembrane G-

Wildtype TECK provided
protein coupled receptors. Over 40 human chemokines have been described, which

as:
bind to ~17 receptors thus far identified. Chemokine activities can be determined

O15444/CCL25_HUMAN
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

C-C motif chemokine 25
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

(SEQ ID NO: 348)
Humana Press Inc., Totowa, NJ. Inflammatory disorders, cancer, Immune and

vascular disorders

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

SDF1alpha GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession B15791
Members of this family are involved in a similarly diverse range of pathologies

WO0042071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P48061-2/SDF1_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Isoform Alpha of Stromal
protein coupled receptors. Over 40 human chemokines have been described, which

cell-derived factor 1
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(isoform alpha)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 259)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine GRO-
Chemokines are a family of related small, secreted proteins involved in biological

alpha GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15793 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P09341/GROA_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Growth-regulated alpha
chemokines exert their effects by acting on a family of seven transmembrane G-

protein
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 340)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine eotaxin
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15794 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P51671/CCL11_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Eotaxin
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 245)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine MIG
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15803 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

Q07325/CXCL9_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-X-C motif chemokine 9
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 351)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine PF4
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15804 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P02776/PLF4_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Platelet factor 4
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 359)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine I-309
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15805 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P22362/CCL1_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 1
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 360)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine HCC-1
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15806 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

Q16627/CCL14_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C motif chemokine 14
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 361)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine C10
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15807 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

SEQ ID NO: 49 of
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO0042071
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 438)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine CCR-2
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15808 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P41597/CCR2_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-C chemokine receptor
chemokines exert their effects by acting on a family of seven transmembrane G-

type 2
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform A)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 362)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine ENA-
Chemokines are a family of related small, secreted proteins involved in biological

78 GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15809 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P42830/CXCL5_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-X-C motif chemokine 5
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 352)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

GRObeta GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession B15810
Members of this family are involved in a similarly diverse range of pathologies

WO0042071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P19875/CXCL2_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 2
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 338)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine IP-10
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15811 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P02778/CXL10_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

C-X-C motif chemokine 10
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 242)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

SDF1beta GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession B15812
Members of this family are involved in a similarly diverse range of pathologies

WO0042071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P48061/SDF1_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

Stromal cell-derived factor 1
protein coupled receptors. Over 40 human chemokines have been described, which

(isoform beta)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 260)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine GRO
Chemokines are a family of related small, secreted proteins involved in biological

alpha GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B15813 WO0042071
Members of this family are involved in a similarly diverse range of pathologies

P09341/GROA_HUMAN
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Growth-regulated alpha
chemokines exert their effects by acting on a family of seven transmembrane G-

protein
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 340)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

MIP1beta GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession B15831
Members of this family are involved in a similarly diverse range of pathologies

WO0042071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P13236/CCL4_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 4
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 358)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Autoimmune disorders, Immune, Vascular and

Inflammatory disorders

A human C-C chemokine
Chemokines are a family of related small, secreted proteins involved in biological

designated exodus
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

B07939
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

U.S. Pat. No. 6,096,300
chemokines exert their effects by acting on a family of seven transmembrane G-

P78556/CCL20_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 20
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(isoform 1)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

(SEQ ID NO: 248)
Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

L105_7 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96922
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 6,084,071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 4 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9856818
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 437)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Chemotaxis, Gene Therapy, Wound healing

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

L105_3 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96923
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 6,084,071
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

SEQ ID NO: 2 of
chemokines exert their effects by acting on a family of seven transmembrane G-

WO9856818
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 436)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Chemotaxis, Gene Therapy, Wound healing

Human secondary
Chemokines are a family of related small, secreted proteins involved in biological

lymphoid chemokine
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

(SLC) GeneSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession B01434
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO0038706
chemokines exert their effects by acting on a family of seven transmembrane G-

O00585/CCL21_HUMAN
protein coupled receptors. Over 40 human chemokines have been described, which

C-C motif chemokine 21
bind to ~17 receptors thus far identified. Chemokine activities can be determined

(SEQ ID NO: 346)
using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, Vascular and Immune disorders

Human non-ELR CXC
Chemokines are a family of related small, secreted proteins involved in biological

chemokine H174
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y96310 WO0029439
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

O14625/CXL11_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 11
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 363)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and Inflammatory disorders, Cancer,

Haemostatic and thrombolytic activity

Human non-ELR CXC
Chemokines are a family of related small, secreted proteins involved in biological

chemokine IP10 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96311
Members of this family are involved in a similarly diverse range of pathologies

WO0029439
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P02778/CXL10_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 10
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 242)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and Inflammatory disorders, Cancer,

haemostatic and thrombolytic activity

Human non-ELR CXC
Chemokines are a family of related small, secreted proteins involved in biological

chemokine Mig GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96313
Members of this family are involved in a similarly diverse range of pathologies

WO0029439
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

Q07325/CXCL9_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-X-C motif chemokine 9
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 351)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Immune and Inflammatory disorders, Cancer,

haemostatic and thrombolytic activity

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

Ckbeta-7 GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96280
Members of this family are involved in a similarly diverse range of pathologies

WO0028035
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

FIG. 1 of WO0028035
chemokines exert their effects by acting on a family of seven transmembrane G-

(SEQ ID NO: 439)
protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing, inflammatory and

immunoregulatory disorders

Human chemokine MIP-
Chemokines are a family of related small, secreted proteins involved in biological

1alpha GeneSeq
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

Accession Y96281
Members of this family are involved in a similarly diverse range of pathologies

WO0028035
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

P10147/CCL3_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 3
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 364)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing, inflammatory and

immunoregulatory disorders

Human mature chemokine
Chemokines are a family of related small, secreted proteins involved in biological

Ckbeta-7 (optionally
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

truncated) GenSeq
Members of this family are involved in a similarly diverse range of pathologies

Accession Y96282
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

WO0028035
chemokines exert their effects by acting on a family of seven transmembrane G-

FIG. 1 of WO0028035
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 440)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, wound healing, inflammatory and

immunoregulatory disorders

Human chemokine
Chemokines are a family of related small, secreted proteins involved in biological

receptor CXCR3 GeneSeq
processes ranging from Chemokine activities can be determined using assays known

Accession Y79372
in the art: Methods in Molecular Biology, 2000, vol. 138: Soluble CXCR3 polypeptides

WO0018431
may be useful for inhibiting

P49682|CXCR3_HUMAN

C-X-C chemokine receptor

type 3

(isoform 1)

(SEQ ID NO: 240)

Human neurotactin
hematopoiesis, angiogenesis, and leukocyte trafficking. Members of this family are

chemokine like domain
involved in a similarly diverse range of pathologies including inflammation, allergy,

GeneSeq Accession
tissue rejection, viral infection, and tumor biology. The chemokines exert their effects

Y53259
by acting on a family of seven transmembrane G-protein coupled receptors. Over 40

U.S. Pat. No. 6,043,086
human chemokines have been described, which bind to ~17 receptors thus far

P78423/X3CL1_HUMAN
identified. Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Fractalkine
Humana Press Inc., Totowa, NJ. chemokine activities and viral infection.

(SEQ ID NO: 244)

Human CC type
Chemokines are a family of related small, secreted proteins involved in biological

chemokine interleukin C
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

GeneSeq Accession
Members of this family are involved in a similarly diverse range of pathologies

Y57771 JP11302298
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

chemokines exert their effects by acting on a family of seven transmembrane G-

protein coupled receptors. Over 40 human chemokines have been described, which

bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer and infectious diseases

Human CKbeta-9
Chemokines are a family of related small, secreted proteins involved in biological

GeneSeq Accession
processes ranging from hematopoiesis, angiogenesis, and leukocyte trafficking.

B50860
Members of this family are involved in a similarly diverse range of pathologies

U.S. Pat. No. 6,153,441
including inflammation, allergy, tissue rejection, viral infection, and tumor biology. The

O00585/CCL21_HUMAN
chemokines exert their effects by acting on a family of seven transmembrane G-

C-C motif chemokine 21
protein coupled receptors. Over 40 human chemokines have been described, which

(SEQ ID NO: 346)
bind to ~17 receptors thus far identified. Chemokine activities can be determined

using assays known in the art: Methods in Molecular Biology, 2000, vol. 138:

Chemokine Protocols. Edited by: A. E. I. Proudfoot, T. N. C. Wells, and C. A. Power.

Humana Press Inc., Totowa, NJ. Cancer, Autoimmune and inflammatory disorders,

Cardiovascular disorders

Preproapolipo- protein
Apoa-1 participates in the reverse transport of cholesterol from tissues to the liver for

“paris” variant GeneSeq
excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for

Accession W08602
the lecithin cholesterol acyltransferase (Icat). Lipid binding activity can be determined

WO9637608
using assays known in the art, such as, for example, the Cholesterol Efflux Assays of

(SEQ ID NO: 466)
Takahaski et al., P.N.A.S., Vol. 96, Issue 20, 11358-11363, Sep. 28, 1999. Useful

for cardiovascular disorders, cholesterol disorders, and Hyperlipidaemia

Preproapolipo- protein
Apoa-1 participates in the reverse transport of cholesterol from tissues to the liver for

“milano” variant 5,721,114
excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for

SEQ ID NO: 6 of
the lecithin cholesterol acyltransferase (Icat). Lipid binding activity can be determined

U.S. Pat. No. 5,721,114
using assays known in the art, such as, for example, the Cholesterol Efflux Assays of

(SEQ ID NO: 441)
Takahaski et al., P.N.A.S., Vol. 96, Issue 20, 11358-11363, Sep. 28, 1999. Useful

for cardiovascular disorders, cholesterol disorders, and Hyperlipidaemia

Glycodelin-A;
Naturally produced female contraceptive that is removed rapidly from the body

Progesterone- associated
following 2-3 days production. Uses include contraception Glycodelin-A activity can

endometrial protein
be determined using the hemizona assay as described in Oehninger, S., Coddington, C. C.,

GeneSeq Accession
Hodgen, G. D., and Seppala, M (1995) Fertil. Steril. 63, 377-383. Naturally

W00289 WO9628169
derived contraceptive useful for the prevention of pregnancy.

P09466/PAEP_HUMAN

Glycodelin

(SEQ ID NO: 365)

NOGO-A Genbank
NOGO polypeptides are potent inhibitors of neurite growth. Inhibition of Neurite

Accession CAB99248
outgrowth. Antagonists to NOGO polypeptides may promote the outgrowth of

(SEQ ID NO: 366)
neurites, thus inducing regeneration of neurons. NOGO-A polypeptide antagonists

are useful for the promotion of neural growth, which could be useful in the treatment

of neural disorders and dysfunction due to degenerative diseases or trauma; useful

in the treatment of neoplastic diseases of the CNS; induce regeneration of neurons

or to promote the structural plasticity of the CNS.

NOGO-B Genbank
NOGO polypeptides are potent inhibitors of neurite growth. Inhibition of Neurite

Accession CAB99249
outgrowth. Antagonists to NOGO polypeptides may promote the outgrowth of

(SEQ ID NO: 367)
neurites, thus inducing regeneration of neurons. NOGO-B polypeptide antagonists

are useful for the promotion of neural growth, which could be useful in the treatment

of neural disorders and dysfunction due to degenerative diseases or trauma; useful

in the treatment of neoplastic diseases of the CNS; induce regeneration of neurons

or to promote the structural plasticity of the CNS.

NOGO-C Genbank
NOGO polypeptides are potent inhibitors of neurite growth. Inhibition of Neurite

Accession CAB99250
outgrowth. Antagonists to NOGO polypeptides may promote the outgrowth of

(SEQ ID NO: 368)
neurites, thus inducing regeneration of neurons. NOGO-C polypeptide antagonists

are useful for the promotion of neural growth, which could be useful in the treatment

of neural disorders and dysfunction due to degenerative diseases or trauma; useful

in the treatment of neoplastic diseases of the CNS; induce regeneration of neurons

or to promote the structural plasticity of the CNS.

NOGO-66 Receptor
NOGO polypeptides are potent inhibitors of neurite growth, and are thought to

Genbank Accession
mediate their effects through the NOGO-66 Receptor. Inhibition of Neurite outgrowth

AAG53612
by mediating the biological effects of NOGO polypeptides. Soluble NOGO-66

(SEQ ID NO: 369)
receptor polypeptides may promote the outgrowth of neurites, thus inducing

regeneration of neurons. NOGO-66 receptor polypeptides are useful for the

promotion of neural growth, which could be useful in the treatment of neural disorders

and dysfunction due to degenerative diseases or trauma; useful in the treatment of

neoplastic diseases of the CNS; induce regeneration of neurons or to promote the

structural plasticity of the CNS.

Antibodies specific for
These antibodies are useful for the promotion of neurite outgrowth Collapsin activity,

collapsin
which is thought to inhibit the outgrowth of neurites, can be assayed in the presence

U.S. Pat. No. 5,416,197
of antibodies specific for collapsing using assays known in the art, such as, for

Wildtype collapsin has the
example, the collapse assay disclosed by Luo et al., Cell 1993 Oct. 22; 75(2): 217-27

sequence:
Useful for the promotion of neural growth, which could be useful in the treatment of

SEQ ID NO: 2 of 5,416,197
neural disorders and dysfunction due to degenerative diseases or trauma.

(SEQ ID NO: 464)

Humanized Anti-VEGF
These agents have anti-inflammatory and anti-cancer applications VEGF activity can

Antibodies, and fragments
be determined using assays known in the art, such as those disclosed in International

thereof WO9845331
Publication No. WO0045835, for example. Promotion of growth and proliferation of

cells, such as vascular endothelial cells. Antagonists may be useful as anti-

angiogenic agents, and may be applicable for cancer

Humanized Anti-VEGF
These agents have anti-inflammatory and anti-cancer applications VEGF activity can

Antibodies, and fragments
be determined using assays known in the art, such as those disclosed in International

thereof WO0029584
Publication No. WO0045835, for example. Promotion of growth and proliferation of

cells, such as vascular endothelial cells. Hematopoietic and immune disorders.

Antagonists may be useful as anti-angiogenic agents, and may be applicable for

cancer

Membrane bound proteins
Cancer, Immune Disorders These proteins can be used for linking bioactive

GeneSeq. Accession
molecules to cells and for modulating biological activities of cells, using the

Y66631-Y66765
polypeptides for specific targeting. The polypeptide targeting can be used to kill the

WO9963088
target cells, e.g. for the treatment of cancers. These proteins are useful for the

treatment of immune system disorders. Activities can be determined using assay

known in the art, such as, for example, the assays disclosed in International

Publication No. WO0121658.

Secreted and
Cancer, Immune Disorders These proteins can be used for linking bioactive

Transmembrane
molecules to cells and for modulating biological activities of cells, using the

polypeptides GeneSeq
polypeptides for specific targeting. The polypeptide targeting can be used to kill the

Accession B44241-B44334
target cells, e.g. for the treatment of cancers. These proteins are useful for the

WO0053756
treatment of immune system disorders. Activities can be determined using assay

known in the art, such as, for example, the assays disclosed in International

Publication No. WO0121658.

Secreted and
Cancer, Immune Disorders These proteins can be used for linking bioactive

Transmembrane
molecules to cells and for modulating biological activities of cells, using the

polypeptides GeneSeq
polypeptides for specific targeting. The polypeptide targeting can be used to kill the

Accession Y41685-Y41774
target cells, e.g. for the treatment of cancers. These proteins are useful for the

WO9946281
treatment of immune system disorders. Activities can be determined using assay

known in the art, such as, for example, the assays disclosed in International

Publication No. WO0121658.

Interleukin 2 (IL-2)
Metabolic Disease, Type 1 diabetes, Graft-versus-host disease.

SEQ ID NO: 548

Interleukin 15_vA
Obesity, Metabolic Disease, Diabetes.

(IL-15_vA)

SEQ ID NO: 549

Interleukin 15_vB
Obesity, Metabolic Disease, Diabetes.

(IL-15_vB)

SEQ ID NO: 550

Interleukin 15_vC
Obesity, Metabolic Disease, Diabetes.

(IL-15_vC)

SEQ ID NO: 551

Interleukin 15_vD
Obesity, Metabolic Disease, Diabetes.

(IL15_vD)

SEQ ID NO: 552

Interleukin 15_vE
Obesity, Metabolic Disease, Diabetes.

(IL15_vE)

SEQ ID NO: 553

Interleukin 15_vF
Obesity, Metabolic Disease, Diabetes.

(IL15_vF)

SEQ ID NO: 565

Interleukin 22
Metabolic Disease, Diabetic Ulcers, Inflamatory Bowel Diseases.

(IL22)

SEQ ID NO: 554

Fibroblast Growth Factor 1
Diabetes, Metabolic Disease, Obesity.

(FGF1)

SEQ ID NO: 555

Fibroblast Growth Factor
Diabetes, Metabolic Disease, Obesity.

1_vA
See Nature 513, 436-439 (18 Sep. 2014) doi: 10.1038/nature13540.

(FGF1_vA)

SEQ ID NO: 556

Fibroblast Growth Factor
Diabetes, Metabolic Disease, Obesity.

1_vB
See Proc Natl Acad Sci USA. 1991 Apr. 1; 88(7): 2893-2897.

(FGF1_vB)

SEQ ID NO: 557

Fibroblast Growth Factor
Diabetes, Metabolic Disease, Obesity.

1_vC

(FGF1_vC)

SEQ ID NO: 566

Fibroblast Growth Factor
Chronic liver disease, primary biliary cirrhosis, bile acid-induced liver damage.

19_vA
See Cancer Res. 2014 Jun. 15; 74(12): 3306-16. doi: 10.1158/0008-5472.CAN-14-

(FGF19_vA)
0208. Epub 2014 Apr. 11. Regulates bile acid metabolism without tumorigenicity.

SEQ ID NO: 558

Fibroblast Growth Factor
Metabolic Disease, Fibrotic Diseases.

21

(FGF21)

SEQ ID NO: 559

Fibroblast Growth Factor
Hyperphosphatemic familial tumoral calcinosis.

23

(FGF23)

SEQ ID NO: 560

Brain-Derived
Neurological diseases (including Alzheimer's Disease, Autism, Huntington's Disease,

Neurotrophic Factor
Parkinson's Disease, and Depression), obesity, metabolic disease.

(BDNF)

SEQ ID NO: 561

Serpin Family A Member 1
alpha-1-antitrypsin deficiency.

(SERPINA1)

SEQ ID NO: 584

SEQ ID NO: 585

Serpin Peptidase Inhibitor,
Diabetes.

Clade B (Ovalbumin),

Member 1

(SERPINB1)

SEQ ID NO: 562

CASPASE1
Diabetes.

SEQ ID NO: 563

Leukemia Inhibitory Factor
Muscular dystrophy, atherosclerosis, kidney disease

(LIF)

SEQ ID NO: 564

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 1
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK1)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 567
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 2
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK2)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 568
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 3
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK3)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 569
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 3 Sol
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK3_SOL)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 570
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 4
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK4)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 571
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 5
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK5)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 572
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 6
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK6)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 573
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK7)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 574
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 8
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK8)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 575
peptide

Proprotein Convertase
Cardiovascular disease, hypercholesterolemia, heterozygous familial

Subtilisin/Kexin Type 9
hypercholesterolemia (HeFH), atherosclerotic cardiovascular disease such as heart

(PCSK9)
attacks or strokes, increasing the amount of a functional protein, polypeptide or

SEQ ID NO: 576
peptide

Membrane-Bound
IFAP syndrome, increasing the amount of a functional protein, polypeptide or peptide

Transcription Factor

Peptidase, Site 2

(MBTPS2)

SEQ ID NO: 577

Carboxypeptidase E
Endocrine disorders, such as, for example, obesity and infertility;

(CPE)
hyperproinsulinemia; metabolic syndrome, increasing the amount of a functional

SEQ ID NO: 578
protein, polypeptide or peptide

In various embodiments, the nucleic acid drug, including synthetic RNA, is administered is a manner that it effects one or more of keratinocytes and fibroblasts (e.g. causes these cells to express one or more therapeutic proteins). For example, present methods allow for methods in which a patient's cells are used to generate a therapeutic protein and the levels of such protein are tailored by synthetic RNA dosing.

In a specific embodiment, the synthetic RNA targets a soluble protein. In some embodiments, the synthetic RNA targets a protein of one or more of the following families of proteins: transforming growth factor (TGF) beta, bone morphogenetic proteins (BMPs), Fibroblast growth factors (FGFs), vascular endothelial growth factors (VEGFs), and interleukins. The terms “family”, “superfamily”, and “subfamily” can be used interchangeably

In a specific embodiment, the synthetic RNA targets a member of the TGF beta family. TGF-β superfamily proteins comprise cytokines characterized by six-conserved cysteine residues (Lander et al., (2001) Nature, 409:860-921). The human genome contains at least about 42 open reading frames encoding TGF-β superfamily proteins. TGF-β superfamily proteins can at least be divided into the BMP subfamily and the TGF-β subfamily based on sequence similarity and the specific signaling pathways that they activate. In various embodiments, the synthetic RNA targets one or more of TGFs (e.g., TGF-β1, TGF-β2, and TGF-β3), activins (e.g., activin A) and inhibins, macrophage inhibitory cytokine-1 (MIC-1), Mullerian inhibiting substance, anti-Mullerian hormone, and glial cell line derived neurotrophic factor (GDNF).

The TGF-β superfamily comprises a subset of the cysteine knot cytokine superfamily. Additional members of the cysteine knot cytokine superfamily include, but are not limited to, platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF), placenta growth factor (P1GF), Noggin, neurotrophins (BDNF, NT3, NT4, and βNGF), gonadotropin, follitropin, lutropin, interleukin-17, and coagulogen. This family of proteins is also among the targets encompassed by the present invention.

In various embodiments, the present invention relates to targeting a TGF beta family member for treatment or prevention of various immunological disorders, cancer, bronchial asthma, lung fibrosis, heart disease, diabetes, hereditary hemorrhagic telangiectasia, Marfan syndrome, Vascular Ehlers-Danlos syndrome, Loeys-Dietz syndrome, Parkinson's disease, chronic kidney disease, multiple sclerosis and AIDS.

In a specific embodiment, the synthetic RNA targets a member of the BMP family. The BMP subfamily includes, but is not limited to, BMP-2, BMP-3 (osteogenin), BMP-3b (GDF-10), BMP-4 (BMP-2b), BMP-5, BMP-6, BMP-7 (osteogenic protein-1 or OP-1), BMP-8 (OP-2), BMP-8B (OP-3), BMP-9 (GDF-2), BMP-10, BMP-11 (GDF-11), BMP-12 (GDF-7), BMP-13 (GDF-6, CDMP-2), BMP-15 (GDF-9), BMP-16, GDF-1, GDF-3, GDF-5 (CDMP-1), and GDF-8 (myostatin). In various embodiments, the synthetic RNA targets one or more of BMP-2, BMP-3 (osteogenin), BMP-3b (GDF-10), BMP-4 (BMP-2b), BMP-5, BMP-6, BMP-7 (osteogenic protein-1 or OP-1), BMP-8 (OP-2), BMP-8B (OP-3), BMP-9 (GDF-2), BMP-10, BMP-11 (GDF-11), BMP-12 (GDF-7), BMP-13 (GDF-6, CDMP-2), BMP-15 (GDF-9), BMP-16, GDF-1, GDF-3, GDF-5 (CDMP-1), and GDF-8 (myostatin). BMPs are sometimes referred to as Osteogenic Protein (OPs), Growth Differentiation Factors (GDFs), or Cartilage-Derived Morphogenetic Proteins (CDMPs). In a specific embodiment, the synthetic RNA targets one or more BMP fusions (e.g. as described in US Patent Publication No. 2009/0202638, the entire contents of which are hereby incorporated by reference) and/or one or more BMP mutants (e.g. as described in US Patent Publication No. 2011/0039773, the entire contents of which are hereby incorporated by reference).

In various embodiments, the present invention relates to targeting a BMP family member for regenerative medicine or metabolic applications, including without limitation orthopedic applications such as spinal fusions, nonunions and oral surgerie, metabolic disease, prediabetes, diabetes, thermogenesis, insulin sensitivity, insulin resistance, and adipogenesis, including brown-fat adipogenesis. Various other metabolic applications are described elsewhere herein. In various embodiments, the present invention relates to targeting a BMP family member, including BMP-7, for treatment of chronic kidney disease (CKD) and/or to reverse the loss of glomeruli due to sclerosis.

In various embodiments, the present invention relates to targeting a BMP family member to induce proliferation of bone and cartilage in a variety of locations in the body. For example, repair of joints such as knee, elbow, ankle, and finger joints are contemplated by the invention. For example, targeting a BMP family member may result in regenerating cartilage in patients suffering from arthritis or other cartilage degenerating diseases. Further, the invention pertains to treating tears in cartilage due to injury. In addition, the invention is useful for inducing bone growth in patients, for instance, by way of non-limitation, for use in treating patients suffering from bone fractures or breaks, osteoporosis, or patients in need of spinal fusion or for repair of the spine, vertebrae or the like.

In various embodiments, the present invention relates to targeting a BMP family member to induce a developmental cascade of bone morphogenesis and tissue morphogenesis for a variety of tissues in mammals different from bone or bone cartilage. This morphogenic activity includes the ability to induce proliferation and differentiation of progenitor cells, and the ability to support and maintain the differentiated phenotype through the progression of events that results in the formation of bone, cartilage, non-mineralized skeletal or connective tissues, and other adult tissues.

For example, the present invention may be used for treatment to prevent loss of and/or increase bone mass in metabolic bone diseases. General methods for treatment to prevent loss of and/or increase bone mass in metabolic bone diseases using osteogenic proteins are disclosed in U.S. Pat. No. 5,674,844, the entire contents of which are hereby incorporated by reference. Further the present compositions and methods find use in replacing or repairing bone or cartilage at injury sites such as bone breaks, bone fractures, and cartilage tears, periodontal tissue regeneration (e.g. general methods for periodontal tissue regeneration using osteogenic proteins are disclosed in U.S. Pat. No. 5,733,878, the entire contents of which are hereby incorporated by reference), liver regeneration, including following a partial hepatectomy (see, e.g., U.S. Pat. No. 5,849,686, the entire contents of which are hereby incorporated by reference), treatment of chronic renal failure (see, e.g., U.S. Pat. No. 6,861,404, the entire contents of which are hereby incorporated by reference), enhancing functional recovery following central nervous system ischemia or trauma (see, e.g. U.S. Pat. No. 6,407,060, the entire contents of which are hereby incorporated by reference), inducing dendritic growth (see, e.g., U.S. Pat. No. 6,949,505, the entire contents of which are hereby incorporated by reference), inducing neural cell adhesion (see, e.g., U.S. Pat. No. 6,800,603, the entire contents of which are hereby incorporated by reference), and treatment and prevention of Parkinson's disease (see, e.g., U.S. Pat. No. 6,506,729, the entire contents of which are hereby incorporated by reference). As another example, the present compositions and methods, including when targeting one or more BMPs, can be used to induce dentinogenesis. To date, the unpredictable response of dental pulp tissue to injury is a basic clinical problem in dentistry. Using standard dental surgical procedures, small areas (e.g., 2 mm) of dental pulps can be surgically exposed by removing the enamel and dentin immediately above the pulp (by drilling) of sample teeth, performing a partial amputation of the coronal pulp tissue, inducing hemostasis, application of the pulp treatment, and sealing and filling the cavity by standard procedures.

In various embodiments, the present invention relates to targeting a BMP family member for the treatment of one or more metabolic-related disorders as described herein.

In a specific embodiment, the synthetic RNA targets a member of the FGF family. FGFs are a family of growth factors, with members involved in angiogenesis, wound healing, embryonic development and various endocrine signaling pathways. In various embodiments, any one of the following FGFs are targets of the invention: FGF1, FGF2, FGF3, FGF4, FGF5, FGF6, FGF7, FGF8, FGF9, FGF10, FGF11, FGF12, FGF13, FGF14 (FGF11, FGF12, FGF13, and FGF14 being FGF homologous factors 1-4 (FHF1-FHF4)), FGF16, FGF17, FGF18, FGF19 (aka FHF15/19), FGF20, FGF21, FGF22 and FGF22. In some embodiments, the synthetic RNA targets a cognate receptor of any of the above FGFs. In various embodiments, the present invention relates to targeting a FGF family member to a treat or prevent disease or disorder associated with abnormal function and/or expression of an FGF, a metabolic disease or disorder (e.g., diabetes, obesity, dyslipidemia, hyperglycemia, hyperinsulinemia, hypertension, hepatosteaotosis such as non-alcoholic steatohepatitis (NASH) etc.), cancer, a disease or disorder associated with impaired lipid metabolism a disease or disorder associated with impaired renal function, a disease or disorder associated with impaired hepatic function, abnormal cell proliferation, a vascular disease or disorder (e.g., coronary artery disease, peripheral artery disease, atherosclerosis, abdominal aortic aneurysm, a blood clot, deep vein thrombosis, venous stasis disease, phlebitis, varicose veins etc.), angiogenesis, atherosclerosis, a cardiovascular disease or disorder, a disease or disorder associated with impaired blood vessel formation, a disease or disorder associated with impaired cell signaling, a disease or disorder associated with impaired kinase activity, and a disease or disorder associated with impaired uptake of glucose into adipocytes.

In a specific embodiment, the synthetic RNA targets a member of the VEGF family. In some embodiments, the target is one or more of VEGF-A (including all isoforms, e.g. VEGF121, VEGF165 and VEGF189), placenta growth factor (PGF, including all isoforms, e.g. PGF-1, PGF-2, and PGF-3), VEGF-B, VEGF-C and VEGF-D, and any variants thereof (see, e.g. U.S. Pat. No. 9,078,860, the entire contents of which are hereby incorporated by reference). In some embodiments, the synthetic RNA targets a cognate receptor of any of the above VEGFs. The present invention also encompasses as targets VEGF-related proteins including orf virus-encoded VEGF-like proteins referred to as VEGF-E and a series of snake venoms referred to as VEGF-F. VEGFs and VEGF-related proteins are members of the Platelet Derived Growth Factor (PDGF) supergene family of cystine knot growth factors. All members of the PDGF supergene family share a high degree of structural homology with PDGF.

In various embodiments, the present invention relates to targeting a VEGF family member to treat diseases and conditions associated with angiogenesis, including but not limited to, solid tumor cancers, hemangiomas, rheumatoid arthritis, osteoarthritis, septic arthritis, asthma, atherosclerosis, idiopathic pulmonary fibrosis, vascular restenosis, arteriovenous malformations, meningiomas, neovascular glaucoma, psoriasis, Kaposi's Syndrome, angiofibroma, hemophilic joints, hypertrophic scars, Osler-Weber syndrome, pyogenic granuloma, retrolental fibroplasias, scleroderma, trachoma, von Hippel-Lindau disease, vascular adhesion pathologies, synovitis, dermatitis, neurological degenerative diseases, preeclampsia, unexplained female infertility, endometriosis, unexplained male infertility, pterygium, wounds, sores, skin ulcers, gastric ulcers, and duodenal ulcers. In various embodiments, the present invention relates to targeting a VEGF family member to treat angiogenesis-associated eye diseases, including without limitation any eye disease associated with abnormal intraocular neovascularization, including but not limited to retinopathy of prematurity, diabetic retinopathy, retinal vein occlusion, and age-related macular degeneration, as well diabetic macular edema and retinal vein occlusion. In an embodiment, the present compositions and methods relate to the treatment of wet age-related macular degeneration.

In a specific embodiment, the synthetic RNA targets a member of the interleukin family. The interleukins represent a large group of cytokines with diverse functions and were first characterized by expression in leukocytes and have since been shown to be expressed in a wide variety of cells, for example macrophages, TH-1 and TH-2 cells, T-lymphocytes, monocytes and bone marrow stroma. Broadly, the function of the immune system depends in a large part on the expression and function of the interleukins. In some embodiments, the target is one or more of interleukins 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 35 and 36, and, within each species of interleukin, various isotypes and/or interleukin receptors (e.g., IL-1R, IL-2R, IL-3R, IL-4R, IL-5R, IL-6R, IL-7R, IL-8R, IL-9R, IL-10R, IL-11R, IL-12R, IL-13R, IL-14R, IL-15R, IL-16R, IL-17R, IL-18R, IL-19R, IL-20R, IL-21R, IL-22R, IL-23R, IL-24R, IL-25R, IL-26, IL-27R, IL-28R, IL-29R, IL-30R, IL-31R, IL-32R, IL-33R, IL-34R, IL-35R, and IL-36R). Ina specific embodiment, both IL-15 and IL-15R (e.g., IL-15RA) are targeted. Without wishing to be bound by theory, it is believed that the targeting of both interleukin (e.g., IL-15) and its cognitive interleukin receptor (e.g., IL-15RA) results in synergistic beneficial effects. In various embodiments, the present invention relates to targeting a member of the interleukin family to treat cancer, inflammatory, respiratory, autoimmune, cardiovascular, neurological, metabolic, and/or proliferative diseases, disorders, and/or conditions in a subject or organism, as described herein. In a specific embodiment, the present invention relates to targeting a member of the interleukin family to treat cancer. In a specific embodiment, the present invention relates to targeting a member of the interleukin family to treat rheumatoid arthritis.

In a specific embodiment, the synthetic RNA targets an EPO gene or a derivative thereof (e.g. SEQ ID NO: 164, SEQ ID NO: 165, SEQ ID NO: 166, and SEQ ID NO: 167). Some embodiments are related to NOVEPOETIN protein (SEQ ID NO: 167). Other embodiments are related to NOVECRIT (SEQ ID NO: 168).

Erythropoietin can stimulate erythropoiesis in anemic patients with chronic renal failure in whom the endogenous production of erythropoietin is impaired. Without being bound by theory, because of the length of time often required for erythropoiesis (several days for erythroid progenitors to mature and be released into the circulation), a clinically significant increase in hemoglobin is usually not observed in less than two weeks and may require up to ten weeks in some patients. The present methods and compositions provide, in some embodiments, more rapid therapeutic effect. The present methods and compositions provide, in some embodiments, more rapid therapeutic effect, for instance, when compared to wild type EPO and/or EPO without non-canonical nucleotides and/or EPO delivered as a protein biologic. The present methods and compositions provide, in some embodiments, a sustained therapeutic effect. The present methods and compositions provide, in some embodiments, a sustained therapeutic effect, for instance, when compared to wild type EPO and/or EPO without non-canonical nucleotides and/or EPO delivered as a protein biologic.

For instance, for EPO, the present methods and compositions provide a clinically significant increase in hematocrit in less than about 6 weeks, or less than about 5 weeks, or less than about 4 weeks, or less than about 3 weeks, or less than about 2 weeks, or less than about 1 week. In some embodiments, the present methods and compositions provide a clinically significant increase in hematocrit in about 2 weeks, or about 10 days, or about 1 week, or about 3 days, or about 1 day. In various embodiments, the present methods and compositions accelerate the process by which erythroid progenitors mature and are released into the circulation.

In some embodiments, the present EPO-related compositions find use in decreasing the dose and/or frequency of administration when compared to wild type EPO and/or EPO without non-canonical nucleotides and/or EPO delivered as a protein biologic. For instance, the present EPO-related compositions may find use in treatment regimens for the diseases disclosed herein (including, without limitation, one or more anemias) that involve administration on a monthly, or biweekly, or weekly basis. In some embodiments, therefore, the present EPO-related compositions reduce the need for daily, or, in some embodiments, weekly, administration. In some embodiments, the present EPO-related compositions require lower maintenance doses as compared to wild type EPO and/or EPO without non-canonical nucleotides and/or EPO delivered as a protein biologic. Certain embodiments are particularly useful for treating chemotherapy-induced anemia. Other embodiments are particularly useful for treating anemia associated with inflammation, including, but not limited to, rheumatoid arthritis.

In some embodiments, the present the present methods and compositions provide a fast and robust response that obviates the need for RBC transfusion. For instance, in some embodiments, the present methods and compositions allow for treatment of patients do not consent to transfusions.

In some embodiments, the present methods and compositions increase the rate of increase in hematocrit. In some embodiments, the present methods and compositions maintain elevated hematocrits (e.g. of 25%, or 30%, or 35%, or 40% or more) for a sustained period (e.g. about 1 month, or about 2 months, or about 3 months, or about 4 months, or about 5 months, or about 6 months, or about 9 months).

In some embodiments, the present methods and compositions stimulate red blood cell production. In some embodiments, the present methods and compositions stimulate division and differentiation of committed erythroid progenitors in the bone marrow.

In some embodiments, including, without limitation, when targeting EPO, the present invention relates to the treatment of one or more of anemia, including anemia resulting from resulting from chronic kidney disease (e.g. from dialysis) and/or chemotherapy and/or HIV treatment (e.g. Zidovudine (INN) or azidothymidine (AZT)), inflammatory bowel disease (e.g. Crohn's disease and ulcer colitis), anemia linked to inflammatory conditions (e.g. arthritis, lupus, IBD), anemia linked to diabetes, schizophrenia, cerebral malaria, as aplastic anemia, and myelodysplasia from the treatment of cancer (e.g. chemotherapy and/or radiation), and various myelodysplastic syndrome diseases (e.g. sickle cell anemia, hemoglobin SC disease, hemoglobin C disease, alpha- and beta-thalassemias, neonatal anemia after premature birth, and comparable conditions).

In some embodiments, including, without limitation, when targeting EPO, the present invention relates to the treatment of, or a patient having one or more of cancer, heart failure, autoimmune disease, sickle cell disease, thalassemia, blood loss, transfusion reaction, diabetes, vitamin B12 deficiency, collagen vascular disease, Shwachman syndrome, thrombocytopenic purpura, Celiac disease, endocrine deficiency state such as hypothyroidism or Addison's disease, autoimmune disease such as Crohn's Disease, systemic lupus erythematosis, rheumatoid arthritis or juvenile rheumatoid arthritis, ulcerative colitis immune disorders such as eosinophilic fasciitis, hypoimmunoglobulinemia, or thymoma/thymic carcinoma, graft vs. host disease, preleukemia, Nonhematologic syndrome (e.g. Down's, Dubowwitz, Seckel), Felty syndrome, hemolytic uremic syndrome, myelodysplasic syndrome, nocturnal paroxysmal hemoglobinuria, osteomyelofibrosis, pancytopenia, pure red-cell aplasia, Schoenlein-Henoch purpura, malaria, protein starvation, menorrhagia, systemic sclerosis, liver cirrhosis, hypometabolic states, congestive heart failure, chronic infections such as HIV/AIDS, tuberculosis, oseomyelitis, hepatitis B, hepatitis C, Epstein-barr virus or parvovirus, T cell leukemia virus, bacterial overgrowth syndrome, fungal or parasitic infections, and/or red cell membrane disorders such as hereditary spherocytosis, hereditary elliptocytosis, heriditray pyrpoikilocytosis, hereditary stomatocytosis, red cell enzyme defects, hypersplenism, immune hemolysis or paroxysmal nocturnal hemoglobinuria.

In some embodiments, including, without limitation, when targeting EPO, the present invention relates to the treatment of, or a patient having anemia, i.e. a condition in which the number of red blood cells and/or the amount of hemoglobin found in the red blood cells is below normal. In various embodiments, the anemia may be acute or chronic. For example, the present anemias include but are not limited to iron deficiency anemia, renal anemia, anemia of chronic diseases/inflammation, pernicious anemia such as macrocytic achylic anemia, juvenile pernicious anemia and congenital pernicious anemia, cancer-related anemia, chemotherapy-related anemia, radiotherapy-related anemia, pure red cell aplasia, refractory anemia with excess of blasts, aplastic anemia, X-lined siderobalstic anemia, hemolytic anemia, sickle cell anemia, anemia caused by impaired production of ESA, myelodysplasia syndromes, hypochromic anemia, microcytic anemia, sideroblastic anemia, autoimmune hemolytic anemia, Cooley's anemia, Mediterranean anemia, Diamond Blackfan anemia, Fanconi's anemia and drug-induced immune hemolytic anemia. Anemia may cause serious symptoms, including hypoxia, chronic fatigue, lack of concentration, pale skin, low blood pressure, dizziness and heart failure.

In some embodiments, the anemia is induced by chemotherapy is one cause of anemia. For instance, the chemotherapy may be any myelosuppressive chemotherapy. In some embodiments, the chemotherapy is one or more platinum-based drugs including cisplatin (e.g. PLATINOL) and carboplatin (e.g. PARAPLATIN). In some embodiments, the chemotherapy is any one of the agents described herein. In some embodiments, the chemotherapy is any agent described in Groopman et al. J Natl Cancer Inst (1999) 91 (19): 1616-1634, the contents of which are hereby incorporated by reference in their entireties. In some embodiments, the present compositions and methods are used in the treatment of chemotherapy-related anemia in later stage cancer patients (e.g. a stage IV, or stage III, or stage II cancer). In some embodiments, the present compositions and methods are used in the treatment of chemotherapy-related anemia in cancer patients receiving dose-dense chemotherapy or other aggressive chemotherapy regimens.

In some embodiments, the present invention relates to the treatment of anemia in a patient having one or more blood-based cancers, such as leukemia, lymphoma, and multiple myeloma. Such cancers may affect the bone marrow directly. Further, the present invention relates to metastatic cancer that has spread to the bone or bone marrow. In some embodiments, the present invention relates to the treatment of anemia in a patient undergoing radiation therapy. Such radiation therapy may damage the bone marrow, lowering its ability to make red blood cells. In further embodiments, the present invention relates to the treatment of anemia in a patient having a reduction or deficiency of one or more of iron, vitamin B12, and folic acid. In further embodiments, the present invention relates to the treatment of anemia in a patient having excessive bleeding including without limitation, after surgery or from a tumor that is causing internal bleeding. In further embodiments, the present invention relates to the treatment of anemia in a patient having anemia of chronic disease.

In some embodiments, the present invention relates to the treatment of anemia resulting from chronic renal failure. In some embodiments, the present invention relates to the treatment of anemia resulting from the use of one or more renal replacement therapies, inclusive of dialysis, hemodialysis, peritoneal dialysis, hemofiltration, hemodiafiltration, and renal transplantation.

In some embodiments, the present invention relates to the treatment of anemia in patients with chronic kidney disease who are not on dialysis. For instance, the present invention relates to patients in stage 1 CKD, or stage 2 CKD, or stage 3 CKD, or stage 4 CKD, or stage 5 CKD. In some embodiments, the present patient is stage 4 CKD or stage 5 CKD. In some embodiments, the present patient has undergone a kidney transplant. In some embodiments, the present invention relates to the treatment of anemia is a patient having an acute kidney injury (AKI).

In various embodiments, the present compositions and methods are used to reduce or eliminate fatigue, dizziness, and shortness of breath in a patient.

In various embodiments, the present compositions and methods are used to treat a patient presenting with hyporesponse or resistance to erythropoiesis stimulating agent therapy. In some embodiments, hyporesponsiveness to erythropoietin or ESA-resistant anemia refers to the presence of at least one of the following conditions: i) a significant decrease in hemoglobin levels at a constant dose of ESA treatment, ii) a significant increase in the ESA dose requirement to achieve or maintain a certain hemoglobin level, iii) a failure to raise the hemoglobin level to the target range despite the ESA dose equivalent to erythropoietin greater than 150 IU/kg/week or 0.75 mg/kg/week of darbepoeitn-alpha or continued need for such high dose of ESA to maintain the target hemoglobin level. For example, approximately 5-10% of patients with CDK demonstrate hyporesponsiveness to ESA, defined as a continued need for greater than 300 IU/kg per week erythropoietin or 1.5 ng/kg per week darbepoetin administered by the subcutaneous route.

In various embodiments, the present compositions and methods mitigate the need for dose-escalation of the erythropoiesis stimulating agent therapy and therefore, optionally, avoid side effects (e.g. flu-like symptoms such as joint pains, weakness, dizziness and tiredness, skin irritation, increased risk of adverse cardiovascular complications).

In various embodiments, the present compositions and methods are used to maintain a hemoglobin level of about 12.5 to 13 g/dL. In various embodiments, the present compositions and methods are used in patients having hemoglobin levels of below about 12 g/dL, or about 11 g/dL, or about 10 g/dL, or about 9 g/dL, or about 8 g/dL, or about 7 g/dL, or about 6 g/dL, or about 5 g/dL. In various embodiments, the present compositions and methods are used in patients having iron blood test scores that indicate blood pathology, e.g. a ferritin score of below about 200 ng/L and/or a transferrin saturation score below about 30%.

In various embodiments, the present compositions and methods are used to increase or maintain hemoglobin levels at a target level ranging from 9 to 10 g/dL, at a target level ranging from 9 g/dL to 11 g/dL, at a target level ranging from 9 g/dL to 12 g/dL, at a target level ranging from 9 g/dL to 14 g/dL, at a target level ranging from 10 g/dL to 14 g/dL, or at a target level ranging from 12 g/dL to 14 g/dL.

In various embodiments, the present compositions and methods are used to bring a patient's hemoglobin levels to normal. In various embodiments, normal hemoglobin ranges for humans are about 14-18 g/dl for men and 12-16 for women g/dl with the average hemoglobin value for men at about 16 g/dL and for women at about 14 g/dL.

In some embodiments, for instance when targeting EPO, the present invention relates to the treatment of anemia of one or more of the following toxicity grading criteria (e.g. NCI Common Toxicity Criteria): grade 1 (mild), 10.0 g hemoglobin/dL to within normal limits; grade 2 (moderate), 8.0-10.0 g of hemoglobin/dL; grade 3 (serious or severe), 6.5-7.9 g of hemoglobin/dL; and grade 4 (life threatening), less than 6.5 g of hemoglobin/dL. In various embodiments, the present invention brings an increase in toxicity grading criteria by about 1 point, or about 2 points, or about 3 points, or about 4 points. In various embodiments, the present invention results in a patient having a level of 0 or 1. In various embodiments, the present compositions and methods improve anemia as assessed by one or more scales described in Groopman et al. J Natl Cancer Inst (1999) 91 (19): 1616-1634, the entire contents of which are hereby incorporated by reference in their entireties.

In some embodiments, when targeting EPO, the present invention relates to combination therapy with one or more EPOs. For example, the present compositions may provide a sustained effect that can supplement a fast action of another EPO. In some embodiments, the present compositions are used as an adjuvant to other EPOs. In some embodiments, the present compositions are used as a maintenance therapy to other EPOs Other EPOs include the following: epoetin alfa, including without limitation, DARBEPOETIN (ARANESP), EPOCEPT (LUPIN PHARMA), NANOKINE (NANOGEN PHARMACEUTICAL), EPOFIT (INTAS PHARMA), EPOGEN (AMGEN), EPOGIN, EPREX, (JANSSEN-CILAG), BINOCRIT (SANDOZ), PROCRIT; epoetin beta, including without limitation, NEORECORMON (HOFFMANN-LA ROCHE), RECORMON, Methoxy polyethylene glycol-epoetin beta (MIRCERA, ROCHE); epoetin delta, including without limitation, DYNEPO (erythropoiesis stimulating protein, SHIRE PLC); epoetin omega, including without limitation, EPOMAX; epoetin zeta, including without limitation, SILAPO (STADA) and RETACRIT (HOSPIRA) and other EPOs, including without limitation, EPOCEPT (LUPIN PHARMACEUTICALS), EPOTRUST (PANACEA BIOTEC LTD), ERYPRO SAFE (BIOCON LTD.), REPOITIN (SERUM INSTITUTE OF INDIA LIMITED), VINTOR (EMCURE PHARMACEUTICALS), EPOFIT (INTAS PHARMA), ERYKINE (INTAS BIOPHARMACEUTICA), WEPDX (WOCKHARDT BIOTECH), ESPOGEN (LG LIFE SCIENCES), RELIPOIETIN (RELIANCE LIFE SCIENCES), SHANPOIETIN (SHANTHA BIOTECHNICS LTD), ZYROP (CADILA HEALTHCARE LTD.), EPIAO (RHUEPO) (SHENYANG SUNSHINE PHARMACEUTICAL CO. LTD), CINNAPOIETIN (CINNAGEN).

In some embodiments, when targeting EPO, the present invention relates to combination therapy with a blood transfusion. For instance, the present compositions may supplement a blood transfusion. In some embodiments, when targeting EPO, the present invention relates to combination therapy with iron supplements.

In some embodiments, the present invention also relates to the following protein targets, e.g. in the treatment of disease: growth hormone (GH) e.g. human and bovine growth hormone, growth hormone-releasing hormones; interferon including α-, β-, or γ-interferons, etc., interleukin-I; interleukin-II; erythropoietin including α- and β-erythropoietin (EPO), granulocyte colony stimulating factor (GCSF), granulocyte macrophage colony stimulating factor (GM-CSF), anti-agiogenic proteins (e.g., angiostatin, endostatin) PACAP polypeptide (pituitary adenylate cyclase activating polypeptide), vasoactive intestinal peptide (VIP), thyrotrophin releasing hormone (TRH), corticotropin releasing hormone (CRH), vasopressin, arginine vasopressin (AVP), angiotensin, calcitonin, atrial naturetic factor, somatostatin, adrenocorticotropin, gonadotropin releasing hormone, oxytocin, insulin, somatotropin, plasminogen tissue activator, coagulation factors including coagulation factors VIII and IX, glucosylceramidase, sargramostim, lenograstin, filgrastin, dornase-α, molgramostim, PEG-L-asparaginase, PEG-adenosine deaminase, hirudin, eptacog-α (human blood coagulation factor Vila) nerve growth factors, transforming growth factor, epidermal growth factor, basic fibroblast growth factor, VEGF; heparin including low molecular weight heparin, calcitonin; antigens; monoclonal antibodies; vancomycin; desferrioxamine (DFO); parathyroid hormone, an immunogen or antigen, and an antibody such as a monoclonal antibody.

In some embodiments, the present methods allow for effective additional therapeutic agent (e.g. those described herein) activity and/or targeting to a cell and/or tissue of interest. For example, the present synthetic RNA can lead to increased expression of one or more targeting molecules that direct an additional therapeutic to the location of therapy. For example, the additional therapeutic agent may have a binding partner that the synthetic RNA encodes. For example, the synthetic RNA may induce the expression of an antigen that directs the therapeutic activity of an antibody that may be used in combination (e.g. herceptin, rituxan, campath, gemtuzumab, herceptin, panorex, rituximab, bexxar, edrecolomab, alemtuzumab, mylotrag, IMC-C225, smartin 195, and mitomomab). In some embodiments, the synthetic RNA can be injected directly into one or more of the tumors described herein and home the therapeutic antibody to the tumor.

In some embodiments, the present methods allow for effective additional therapeutic agent generation, especially when the additional therapeutic agent is a prodrug, for example, to produce an active form of the drug. In some embodiments, the synthetic RNA can be injected directly into one or more of the tumors described herein and home the prodrug to the tumor. For instance, the synthetic RNA may encode an enzyme that catalyzes the localized conversion of a non-toxic, systemically delivered agent into a potent chemotherapeutic agent. By way of illustration (note that any of the prodrugs or drugs listed herein are additional agents as used herein):

Enzyme
Prodrug
Drug

aldehyde oxidases
5-Ethynyl-2(1H)-pyrimidinone
5-Ethynyluracil

aldehyde oxidases
IPdR
IUdR

aldehyde oxidases
5-FP
5-FU

amino acid oxidases
d-alanine
Hydrogen peroxide

amino acid oxidases
SeCys conjugates
Selenols and hydrogen peroxide

cytochrome P450 reductase
Menadione
Semiquinone radical

cytochrome P450 reductase
Mitomycin C
Quinone methide intermediate

cytochrome P450 reductase
Tirapazamine
Nitroxide radical

cytochrome P450 reductase
EO9
Unidentified semiquinone radical

DT-diaphorase
Streptonigrin
Unidentified

DT-diaphorase
Mitomycin C
Quinone methide intermediate

DT-diaphorase
CB 1954
5-(Aziridin-1-yl)-4-hydroxyl-amino-2-

nitrobenzamide

DT-diaphorase
Diaziquone
Semiguinone radical^a

cytochrome P450
Ipomeanol
Unidentified (possibly furan epoxide)

cytochrome P450
Ftorafur (tegafur)
5-FU

cytochrome P450
Dacarbazine
HHMTIC

cytochrome P450
Trofosfamide
Trofosfamide mustard

cytochrome P450
Ifosfamide
Isophosphamide mustard

cytochrome P450
Cyclophosphamide
Phosphoramide mustard

cytochrome P450
AQ4N
AQ4

tyrosinase
2,4-Dihydroxyphenylalanine
6-Hydroxydopa

tyrosinase
4-S-CAP
BQ

tyrosinase
GHB
GBQ

tyrosinase
Substituted phenols
Orthoquinones

tyrosinase
Phenyl mustards
Phenol mustard

tyrosinase
Urea mustards
Unidentified

glutathione S-transferase
TER286
Aziridinium agent

glutathione S-transferase
S-CPHC-ethylsulfoxide
S-CPHC-glutathione

glutathione S-transferase
PTA
6-MP

carboxylesterase
CPT-11
SN-38

carboxylesterase
Paclitaxel-2-ethylcarbonate
Paclitaxel

carboxylesterase
Capecitabine
5′-Deoxy-5-fluorocytidine (5-FU)

carboxylesterase
Tertiary amidomethyl esters
Carboxylic acids and amines^a

alkaline phosphatase
Amifostine
WR-1065

alkaline phosphatase
3-AP phosphate
3-AP

β-glucuronidase
BHAMG
pHAM

β-glucuronidase
Epirubicin-glucuronide
Epirubicin

β-glucuronidase
HMR 1826
Doxorubicin

β-glucuronidase
DNR-GA3
Daunorubicin

β-glucuronidase
DOX-GA3
Doxorubicin

β-glucuronidase
Paclitaxel glucuronide
Paclitaxel

β-glucuronidase
5-FU glucuronide
5-FU

cysteine conjugate β-lyase
PC
6-MP

cysteine conjugate β-lyase
GC
6-Thioguanine

cysteine conjugate β-lyase
SeCys conjugate
Selenol

Nitroreductase
CB 1954
5-(Aziridin-1-yl)-4-hydroxyl-amino-2-

nitro- benzamide

Cytochrome P450
4-Ipomeanol
Unidentified (furan epoxide is

speculated)

Cytochrome P450
Ifosfamide
Isophosphoramide mustard

Cytochrome P450
Cyclophosphamide
Phosphoramide mustard

Purine-nucleoside
Fludarabine
2-Fluoroadenine

phosphorylase

Purine-nucleoside
MeP-dR
MeP

phosphorylase

Thymidine kinase
Ganciclovir
Ganciclovir-triphosphate nucleotide

Alkaline phosphatase
Etoposide phosphate
Etoposide

Alkaline phosphatase
Mitomycin C phosphate
Mitomycin C

Alkaline phosphatase
POMP
POM

Alkaline phosphatase
N-(4-phosphonooxy)-
Doxorubicin

phenylacetyl)doxorubicin

β-Glucuronidase
Glucuronidated Nornitrogen mustard
Oxazolidinone

β-Glucuronidase
Glucuronidated 9-amino-
9-Aminocamptothecin

camptothecin

β-Glucuronidase
Glucuronide mustard
Mustard

Carboxypeptidase
Methotrexate-amino acids
Methotrexate

Carboxypeptidase
CMDA
Benzoic acid mustard

Penicillin amidase
DPO
Doxorubicin

Penicillin amidase
MelPO
Melphalan

Penicillin amidase
NHPAP
Palytoxin

Penicillin amidase
N-(phenylacetyl) doxorubicin
Doxorubicin

Penicillin amidase
N-(phenylacetyl) melphalan
Melphalan

β-Lactamase
LY 266070
DAVLBHYD

β-Lactamase
C-DOX
Doxorubicin

β-Lactamase
PRODOX
Doxorubicin

β-Lactamase
CM
Phenylenediamine mustard

β-Lactamase
CCM
Phenylenediamine mustard

β-Lactamase
Cephalosporin-DACCP
DACCP

β-Lactamase
PROTAX
Taxol

β-Lactamase
Cephalosporin mitomycin C
Mitomycin C

β-Lactamase
C-Mel
Melphalan

Cytosine deaminase
5-Fluorocytosine
5-Fluorouracil

Methionine γ-lyase
Selenomethionine
Methylselenol

Methionine γ-lyase
Trifluoromethionine
CSF₂

In certain embodiments, the synthetic RNA may encode an enzyme that catalyzes the conversion of 5-FU and/or Doxorubicin from various prodrugs, as illustrated by the examples below:

5-FU Prodrugs and Enzymes

5-FP
Aldehyde oxidase

Ftorafur
P450

5′-DFUR
Thymidine phosphorylase

5-FU glucuronide
β-Glucuronidase

5-FC
Cytosine deaminase

Doxorubicin Prodrugs and Enzymes

N-(4-phosphono-oxy)-phenylacetyl)
Alkaline phosphatase

doxorubicin

HMR 1826
β-Glucuronidase

DOX-GA3
β-Glucuronidase

DPO
Penicillin amidase

N-(phenyacetyl) doxorubicin
Penicillin amidase

C-DOX
β-Lactamase

PRODOX
β-Lactamase

In some embodiments, the nucleic acid drugs at the doses and regimens described herein may be used in combination with one or more additional agents (aka adjuvant therapy or combination agent). In some embodiments, the nucleic acid drugs at the doses and regimens described herein may be used in a human patient undergoing treatment with one or more additional agents. In some embodiments, the nucleic acid drug is used as an adjuvant or neoadjuvant to any of the additional agents described herein. In some embodiments, the invention pertains to co-administration and/or co-formulation. Any of the compositions described herein may be co-formulated and/or co-administered. In some embodiments, any nucleic acid drug described herein acts synergistically when co-administered with another agent and may be administered at doses that are lower than the doses commonly employed when such agents are used as monotherapy.

In some embodiments, any nucleic acid drug described herein may include derivatives that are modified, i.e., by the covalent attachment of any type of molecule to the composition such that covalent attachment does not prevent the activity of the composition. For example, but not by way of limitation, derivatives include composition that have been modified by, inter alia, glycosylation, lipidation, acetylation, pegylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to a cellular ligand or other protein, etc. Any of numerous chemical modifications can be carried out by known techniques, including, but not limited to specific chemical cleavage, acetylation, formylation, metabolic synthesis of turicamycin, etc. Additionally, the derivative can contain one or more non-classical amino acids. In various embodiments, one or more additional agents (aka adjuvant therapy or combination agent) may be conjugated to any nucleic acid drug described herein.

Contacting a cell with a steroid can suppress the innate immune response to foreign nucleic acids, and can increase the efficiency of nucleic acid delivery and translation. Certain embodiments are therefore directed to contacting a cell with a steroid. Other embodiments are directed to administering a steroid to a patient. Illustrative steroids include corticosteroid steriods. In some embodiments, the steroid is one or more of cortisone, hydrocortisone, prednisone, prednisolone, dexamethasone, triamcinolone, and betamethasone. In one embodiment, the steroid is hydrocortisone. In another embodiment, the steroid is dexamethasone.

Other embodiments are directed to administering to a patient a member of the group: an antibiotic, an antimycotic, and an RNAse inhibitor.

Botulinum toxin type A has been approved by the US Food and Drug Administration (FDA) for the treatment of essential blepharospasm, strabismus and hemifacial spasm in patients over the age of twelve, cervical dystonia, glabellar line (facial) wrinkles and for treating hyperhydrosis and botulinum toxin type B has been approved for the treatment of cervical dystonia. The present compositions may be combined with these toxins in the treatment of these diseases and related diseases. Some embodiments are directed to a nucleic acid drug targeting a neurotoxin. In various embodiments, the neurotoxin is a botulinum toxin or a biologically active fragment, variant, analogue or family-member thereof.

Further the combination of any one of the aforementioned toxins may be used in combination with the present compositions for various cosmetic procedures, including, without limitation, facial wrinkles, hyperkinetic skin lines, glabellar lines, crow's feet, marionette lines, skin disorders, nasolabial folds, blepharospasm, strabismus, hemifacial spasms and sweating disorders. Alternatively, the present compositions may be used to in these cosmetic procedures as a monotherapy.

Certain embodiments are directed to a combination therapy comprising one or more of the therapeutic or cosmetic compositions of the present invention and one or more adjuvant therapies or cosmetic treatments. In certain embodiments, one or more of the therapeutic or cosmetic compositions of the present invention are administered to a subject which is undergoing treatment with one or more adjuvant therapies or cosmetic treatments. Example adjuvant therapies and cosmetic treatments are set forth in Table 3 and Table 5 of U.S. Provisional Application No. 61/721,302, the contents of which are hereby incorporated by reference, and are given by way of example, and not by way of limitation.

TABLE 3

Illustrative Adjuvant Therapies

Example

Therapy/Treatment Class
Disease/Condition
Therapy/Treatment

Acetylcholinesterase inhibitors
Myasthenia gravis, Glaucoma,
Edrophonium

Alzheimer′s disease, Lewy body

dementia, Postural tachycardia syndrome

Angiotensin-converting-enzyme
Hypertension, Congestive heart failure
Perindopril

inhibitor

Alkylating agents
Cancer
Cisplatin

Angiogenesis inhibitors
Cancer, Macular degeneration
Bevacizumab

Angiotensin II receptor
Hypertension, Diabetic nephropathy,
Valsartan

antagonists
Congestive heart failure

Antibiotics
Bacterial infection
Amoxicillin

Antidiabetic drugs
Diabetes
Metformin

Antimetabolites
Cancer, Infection
5-fluorouracil (5FU)

Antisense oligonucleotides
Cancer, Diabetes, Amyotrophic lateral
Mipomersen

sclerosis (ALS), Hypercholesterolemia

Cytotoxic antibiotics
Cancer
Doxorubicin

Deep-brain stimulation
Chronic pain, Parkinson's disease,
N/A

Tremor, Dystonia

Dopamine agonists
Parkinson's disease, Type II diabetes,
Bromocriptine

Pituitary tumors

Entry/Fusion inhibitors
HIV/AIDS
Maraviroc

Glucagon-like peptide-1 agonists
Diabetes
Exenatide

Glucocorticoids
Asthma, Adrenal insufficiency,
Dexamethasone

Inflammatory diseases, Immune diseases,

Bacterial meningitis

Immunosuppressive drugs
Organ transplantation, Inflammatory
Azathioprine

diseases, Immune diseases

Insulin/Insulin analogs
Diabetes
NPH insulin

Integrase inhibitors
HIV/AIDS
Raltegravir

MAO-B inhibitors
Parkinson's disease, Depression,
Selegiline

Dementia

Maturation inhibitors
HIV/AIDS
Bevirimat

Nucleoside analog reverse-
HIV/AIDS, Hepatitis B
Lamivudine

transcriptase inhibitors

Nucleotide analog reverse-
HIV/AIDS, Hepatitis B
Tenofovir

transcriptase inhibitors

Non-nucleoside reverse-
HIV/AIDS
Rilpivirine

transcriptase inhibitors

Pegylated interferon
Hepatitis B/C, Multiple sclerosis
Interferon beta-1a

Plant alkaloids/terpenoids
Cancer
Paclitaxel

Protease inhibitors
HIV/AIDS, Hepatitis C, Other viral
Telaprevir

infections

Radiotherapy
Cancer
Brachytherapy

Renin inhibitors
Hypertension
Aliskiren

Statins
Hypercholesterolemia
Atorvastatin

Topoisomerase inhibitors
Cancer
Topotecan

Vasopressin receptor antagonist
Hyponatremia, Kidney disease
Tolvaptan

Dermal filler
Wrinkles, aged skin
Hyaluronic Acid

Botulinum toxin
Wrinkles, aged skin
botulinum toxin type A

Induction of skin repair
Acne scars, aged skin
Laser treatment,

dermabrasion

In some embodiments, the additional agent is a cytotoxic agent, comprising, in illustrative embodiments, a toxin, a chemotherapeutic agent, a radioisotope, and an agent that causes apoptosis or cell death.

Illustrative cytotoxic agents include, but are not limited to, methotrexate, aminopterin, 6-mercaptopurine, 6-thioguanine, cytarabine, 5-fluorouracil decarbazine; alkylating agents such as mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU), mitomycin C, lomustine (CCNU), 1-methylnitrosourea, cyclothosphamide, mechlorethamine, busulfan, dibromomannitol, streptozotocin, mitomycin C, cis-dichlorodiamine platinum (II) (DDP) cisplatin and carboplatin (paraplatin); anthracyclines include daunorubicin (formerly daunomycin), doxorubicin (adriamycin), detorubicin, carminomycin, idarubicin, epirubicin, mitoxantrone and bisantrene; antibiotics include dactinomycin (actinomycin D), bleomycin, calicheamicin, mithramycin, and anthramycin (AMC); and antimytotic agents such as the vinca alkaloids, vincristine and vinblastine. Other cytotoxic agents include paclitaxel (taxol), ricin, pseudomonas exotoxin, gemcitabine, cytochalasin B, gramicidin D, ethidium bromide, emetine, etoposide, tenoposide, colchicin, dihydroxy anthracin dione, 1-dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, puromycin, procarbazine, hydroxyurea, asparaginase, corticosteroids, mytotane (O,P′-(DDD)), interferons, and mixtures of these cytotoxic agents.

Further cytotoxic agents include, but are not limited to, chemotherapeutic agents such as carboplatin, cisplatin, paclitaxel, gemcitabine, calicheamicin, doxorubicin, 5-fluorouracil, mitomycin C, actinomycin D, cyclophosphamide, vincristine, bleomycin, VEGF antagonists, EGFR antagonists, platins, taxols, irinotecan, 5-fluorouracil, gemcytabine, leucovorine, steroids, cyclophosphamide, melphalan, vinca alkaloids (e.g., vinblastine, vincristine, vindesine and vinorelbine), mustines, tyrosine kinase inhibitors, radiotherapy, sex hormone antagonists, selective androgen receptor modulators, selective estrogen receptor modulators, PDGF antagonists, TNF antagonists, IL-1 antagonists, interleukins (e.g. IL-12 or IL-2), IL-12R antagonists, Toxin conjugated monoclonal antibodies, tumor antigen specific monoclonal antibodies, Erbitux, Avastin, Pertuzumab, anti-CD20 antibodies, Rituxan, ocrelizumab, ofatumumab, DXL625, HERCEPTIN, or any combination thereof. Toxic enzymes from plants and bacteria such as ricin, diphtheria toxin and Pseudomonas toxin may be conjugated to the therapeutic agents (e.g. antibodies) to generate cell-type-specific-killing reagents (Youle, et al., Proc. Nat'l Acad. Sci. USA 77:5483 (1980); Gilliland, et al., Proc. Nat'l Acad. Sci. USA 77:4539 (1980); Krolick, et al., Proc. Nat'l Acad. Sci. USA 77:5419 (1980)).

Other cytotoxic agents include cytotoxic ribonucleases as described by Goldenberg in U.S. Pat. No. 6,653,104. Embodiments of the invention also relate to radioimmunoconjugates where a radionuclide that emits alpha or beta particles is stably coupled to the antibody, or binding fragments thereof, with or without the use of a complex-forming agent. Such radionuclides include beta-emitters such as Phosphorus-32, Scandium-47, Copper-67, Gallium-67, Yttrium-88, Yttrium-90, Iodine-125, Iodine-131, Samarium-153, Lutetium-177, Rhenium-186 or Rhenium-188, and alpha-emitters such as Astatine-211, Lead-212, Bismuth-212, Bismuth-213 or Actinium-225.

Illustrative detectable moieties further include, but are not limited to, horseradish peroxidase, acetylcholinesterase, alkaline phosphatase, beta-galactosidase and luciferase. Further exemplary fluorescent materials include, but are not limited to, rhodamine, fluorescein, fluorescein isothiocyanate, umbelliferone, dichlorotriazinylamine, phycoerythrin and dansyl chloride. Further exemplary chemiluminescent moieties include, but are not limited to, luminol. Further exemplary bioluminescent materials include, but are not limited to, luciferin and aequorin. Further exemplary radioactive materials include, but are not limited to, Iodine-125, Carbon-14, Sulfur-35, Tritium and Phosphorus-32.

The dosage of any additional agent described herein as well as the dosing schedule can depend on various parameters, including, but not limited to, the human patient's general health, and the administering physician's and/or human patient's discretion. Co-administration may be simultaneous or sequential. Any additional agent described herein, can be administered prior to (e.g., about 5 minutes, about 15 minutes, about 30 minutes, about 45 minutes, about 1 hour, about 2 hours, about 4 hours, about 6 hours, about 12 hours, about 24 hours, about 48 hours, about 72 hours, about 96 hours, about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 5 weeks, about 6 weeks, 8 weeks, or about 12 weeks before), concurrently with, or subsequent to (e.g., about 5 minutes, about 15 minutes, about 30 minutes, about 45 minutes, about 1 hour, about 2 hours, about 4 hours, about 6 hours, about 12 hours, about 24 hours, about 48 hours, about 72 hours, about 96 hours, about 1 week, about 2 weeks, about 3 weeks, about 4 weeks, about 5 weeks, about 6 weeks, about 8 weeks, or about 12 weeks after) the administration of the nucleic acid drug to a human patient in need thereof. In various embodiments any agent described herein is administered about 1 minute apart, about 10 minutes apart, about 30 minutes apart, less than about 1 hour apart, about 1 hour apart, about 1 hour to about 2 hours apart, about 2 hours to about 3 hours apart, about 3 hours to about 4 hours apart, about 4 hours to about 5 hours apart, about 5 hours to about 6 hours apart, about 6 hours to about 7 hours apart, about 7 hours to about 8 hours apart, about 8 hours to about 9 hours apart, about 9 hours to about 10 hours apart, about 10 hours to about 11 hours apart, about 11 hours to about 12 hours apart, no more than about 24 hours apart or no more than about 48 hours apart.

In a specific embodiment, the combination regimen is designed to exploit the finding that the nucleic acid drug dosing and formulation of the present invention has potent effects quickly (e.g. in about 6, or about 12, or about 24, or about 30, or about 36, or about 48 hours) and the effects can last about 7 days, or longer.

The dose of a nucleic acid drug is disclosed herein. In general, the dose of any additional agent that is useful is known to those in the art. For example, doses may be determined with reference Physicians' Desk Reference, 66th Edition, PDR Network; 2012 Edition (Dec. 27, 2011), the contents of which are incorporated by reference in its entirety. In some embodiments, the present invention allows a patient to receive doses that exceed those determined with reference Physicians' Desk Reference. The dosage of any additional agent described herein can depend on several factors including the severity of the condition, whether the condition is to be treated or prevented, and the age, weight, and health of the human patient to be treated. Additionally, pharmacogenomic (the effect of genotype on the pharmacokinetic, pharmacodynamic or efficacy profile of a therapeutic) information about a particular human patient may affect dosage used. Furthermore, the exact individual dosages can be adjusted somewhat depending on a variety of factors, including the specific combination of the agents being administered, the time of administration, the route of administration, the nature of the formulation, the rate of excretion, the particular disease being treated, the severity of the disorder, and the anatomical location of the disorder. Some variations in the dosage can be expected.

Cells, tissues, organs, and organisms, including, but not limited to, humans, have several characteristics that can inhibit or prevent the delivery of nucleic acids, including, for example, the stratum corneum, which can serve as a barrier to foreign organisms and nucleic acids. These characteristics can thus inhibit the effects of therapeutics and cosmetics comprising nucleic acids. It has now been discovered that many of these characteristics can be circumvented or overcome using a patch comprising a flexible membrane and a plurality of needles, and that such a patch can serve as an effective and safe article for the delivery of nucleic acids. Certain embodiments are therefore directed to a nucleic acid delivery patch. In one embodiment, the nucleic acid delivery patch comprises a flexible membrane. In another embodiment, the nucleic acid delivery patch comprises a plurality of needles. In yet another embodiment, the plurality of needles are attached to the flexible membrane. In some embodiments, the patch comprises a nucleic acid. In one embodiment, the nucleic acid is present in solution. In one embodiment, the plurality of needles include one or more needles having a lumen. In another embodiment, the patch further comprises a second flexible membrane. In yet another embodiment, the flexible membrane and the second flexible membrane are arranged to form a cavity. In a further embodiment, the cavity contains a nucleic acid. In a still further embodiment, the membrane comprises one or more holes through which a nucleic acid can pass. In a still further embodiment, one or more holes and one or more needles having a lumen are arranged to allow the passage of a solution containing a nucleic acid through at least one of the one or more holes and through at least one of the one or more needles having a lumen. In some embodiments, the patch is configured to deliver a solution to the skin. In one embodiment, the solution comprises a nucleic acid. In another embodiment, the solution comprises a vehicle. In yet another embodiment, the vehicle is a lipid or lipidoid. In a still further embodiment, the vehicle is a lipid-based transfection reagent.

The cell membrane can serve as a barrier to foreign nucleic acids. It has now been discovered that combining the patch of the present invention with an electric field can increase the efficiency of nucleic acid delivery. Certain embodiments are therefore directed to a nucleic acid delivery patch comprising a plurality of needles, wherein at least two needles form part of a high-voltage circuit. Certain embodiments are directed to an implantable “tattoo” for microneedle delivery (see, e.g. Nature Materials 12, pp 367-376 (2013), the contents of which are hereby incorporated by reference in their entirety). In one embodiment, the high-voltage circuit generates a voltage greater than about 10V. In another embodiment, the high-voltage circuit generates a voltage greater than about 20V. In yet another embodiment, an electric field is produced between two of the needles. In a further embodiment, the magnitude of the electric field is at least about 100V/cm. In a still further embodiment, the magnitude of the electric field is at least about 200V/cm. In some embodiments, the patch is configured to deliver a nucleic acid to the epidermis. In other embodiments, the patch is configured to deliver a nucleic acid to the dermis. In still other embodiments, the patch is configured to deliver a nucleic acid to sub-dermal tissue. In still other embodiments, the patch is configured to deliver a nucleic acid to muscle. Certain embodiments are directed to a nucleic acid delivery patch comprising a plurality of electrodes. In one embodiment, the plurality of electrodes is attached to a flexible membrane. Other embodiments are directed to a nucleic acid delivery patch comprising a rigid structure. In one embodiment, a plurality of electrodes are attached to the rigid structure.

In some embodiments, the compositions described herein are administered using an array of needles covering an affected area of the subject. In some embodiments, the treatment area is mechanically massaged after administration. In some embodiments, the treatment area is exposed to electric pulses after administration. In some embodiments, the electric pulses are between about 10V and about 200V for from about 50 microseconds to about 1 second. In some embodiments, the electric pulses are generated around the treatment area by a multielectrode array.

In some embodiments, the present invention provides a patch delivery system, comprising a non-viral RNA transfection composition enclosed within a membrane, and an array of delivery needles delivering from about 10 ng to about 2000 ng of RNA per treatment area of about 100 cm²or less, or about 50 cm²or less, or about 10 cm²or less, or about 5 cm²or less, or about 1 cm²or less, or about 0.5 cm²or less, or about 0.2 cm²or less. In some embodiments, the non-viral transfection composition contains from about 10 ng to about 2000 ng per injection volume of about 20 μL to about 1 ml. In some embodiments, each needle delivers an injection volume of between 1 μL and 500 μL.

In some embodiments, the delivery patch comprises an acrylic reservoir that holds the nucleic acid drug. In some embodiments, a silicon adhesive is added to create a semisolid suspension of microscopic, concentrated drug cells. Further, some embodiments pride a patch that is associated with one or more enhancers (these include, without limitation, iontophoresis, ultrasound, chemicals including gels, microneedles, sonophoresis, lasers, and electroporatic methods).

In some embodiments, the delivery is effected via a gel, optionally a hydro alcoholic gel containing a combination of enhancers (e.g. COMBIGEL (ANTARES PHARMA)).

In various embodiments, the RNA is delivered using needle arrays. Illustrative needle arrays include, but are not limited to AdminPen 600 and those described in U.S. Pat. Nos. 7,658,728, 7,785,301, and 8,414,548, the entire disclosure of which are hereby incorporated by reference. Other examples of needles include, for example, the 3M™ Hollow Microstructured Transdermal System and the 3M Solid Microstructured Transdermal Systems (sMTS). See, e.g. U.S. Pat. Nos. 3,034,507 and 3,675,766; Microneedles for Transdermal Drug Delivery. Advanced Drug Delivery Reviews. 56: 581-587 (2004); Pharm Res. 2011 January; 28(1): 31-40, the entire contents of which are hereby incorporated by reference in their entireties.

In some embodiments, microneedles and/or microneedle arrays may be used. In various embodiments, the microneedles and/or microneedle arrays may be, without limitation, solid, RNA-coated, dissolving, biodegradable, and/or hollow. In some embodiments, the delivery is effected via a microneedle system, optionally combined with an electronically controlled micropump that delivers the drug at specific times or upon demand. For example, the MACROFLUX (Alza) system may be used.

Other embodiments are directed to a method for delivering a nucleic acid to a cell in vivo comprising applying a nucleic acid to a tissue containing a cell in vivo. In one embodiment, the method further comprises applying a transient electric field in the vicinity of the cell. In another embodiment, the method results in the cell in vivo internalizing the nucleic acid. In yet another embodiment, the nucleic acid comprises synthetic RNA. In a further embodiment, the method further results in the cell internalizing a therapeutically or cosmetically effective amount of the nucleic acid. In one embodiment, the cell is a skin cell. In another embodiment, the cell is a muscle cell. In yet another embodiment, the cell is a dermal fibroblast. In a further embodiment, the cell is a keratinocyte. In a still further embodiment, the cell is a myoblast. In some embodiments, the nucleic acid comprises a protein of interest. In one embodiment, the protein of interest is a fluorescent protein. In another embodiment, the protein of interest is an extracellular-matrix protein. In yet another embodiment, the protein of interest is a member of the group: elastin, collagen, laminin, fibronectin, vitronectin, lysyl oxidase, elastin binding protein, a growth factor, fibroblast growth factor, transforming growth factor beta, granulocyte colony-stimulating factor, a matrix metalloproteinase, an actin, fibrillin, microfibril-associated glycoprotein, a lysyl-oxidase-like protein, platelet-derived growth factor, a lipase, an uncoupling protein, thermogenin, filaggrin, a fibroblast growth factor, an antibody, and a protein involved with pigment production. In some embodiments, the method further comprises delivering the nucleic acid to the epidermis. In other embodiments, the method further comprises delivering the nucleic acid to the dermis. In still other embodiments, the method further comprises delivering the nucleic acid below the dermis. In one embodiment, the delivering is by injection. In another embodiment, the delivering is by injection using a micro-needle array. In yet another embodiment, the delivering is by topical administration. In a further embodiment, the delivering comprises disruption or removal of a part of the tissue. In a still further embodiment, the delivering comprises disruption or removal of the stratum corneum. In some embodiments, the nucleic acid is present in solution. In one embodiment, the solution comprises a growth factor. In another embodiment, the growth factor is a member of the group: a fibroblast growth factor and a transforming growth factor. In yet another embodiment, the growth factor is a member of the group: basis fibroblast growth factor and transforming growth factor beta. In other embodiments, the solution comprises cholesterol.

In another embodiment, the method further comprises contacting the cell with one or more nucleic acid molecules. In yet another embodiment, at least one of the one or more nucleic acid molecules encodes a protein of interest. In a further embodiment, the method results in the cell expressing the protein of interest. In a still further embodiment, the method results in the cell expressing a therapeutically or cosmetically effective amount of the protein of interest.

In another embodiment, the cell is contacted with a nucleic acid molecule. In yet another embodiment, the method results in the cell internalizing the nucleic acid molecule. In a further embodiment, the method results in the cell internalizing a therapeutically or cosmetically effective amount of the nucleic acid molecule. In one embodiment, the nucleic acid encodes a protein of interest. In one embodiment, the nucleic acid molecule comprises a member of the group: a dsDNA molecule, a ssDNA molecule, a RNA molecule, a dsRNA molecule, a ssRNA molecule, a plasmid, an oligonucleotide, a synthetic RNA molecule, a miRNA molecule, an mRNA molecule, and an siRNA molecule. In various embodiments, the RNA comprises one or more non-canonical nucleotides.

In some embodiments, the present invention relates to one or more administration techniques described in U.S. Pat. Nos. 5,711,964; 5,891,468; 6,316,260; 6,413,544; 6,770,291; and 7,390,780, the entire contents of which are hereby incorporated by reference in their entireties.

The invention also provides kits that can simplify the administration of the nucleic acid drugs described herein and/or any additional agent described herein. An illustrative kit of the invention comprises a nucleic acid drug and/or any additional agent described herein in unit dosage form. In one embodiment, the unit dosage form is a container, such as a pre-filled syringe, which can be sterile, containing any agent described herein and a pharmaceutically acceptable carrier, diluent, excipient, or vehicle. The kit can further comprise a label or printed instructions instructing the use of any agent described herein. The kit or one or more components of the kit may be stored at room temperature, about 4° C., about −20° C., about −80° C., or about −196° C. The kit may also include a lid speculum, topical anesthetic, and a cleaning agent for the administration location. The kit can also further comprise one or more additional agent described herein. In one embodiment, the kit comprises a container containing an effective amount of a nucleic acid drug as disclosed herein and an effective amount of another composition, such as an additional agent as described herein. In some embodiments, the unit dosage form is a pre-loaded (a.k.a. pre-dosed or pre-filled) syringe or a pen needle injector (injection pen)). Such unit dosage forms may comprise the effective doses of nucleic acid drug described herein, e.g. about 10 ng to about 2000 ng, e.g. about 10 ng, or about 20 ng, or about 50 ng, or about 100 ng, or about 200 ng, or about 300 ng, or about 400 ng, or about 500 ng, or about 600 ng, or about 700 ng, or about 800 ng, or about 900 ng, or about 1000 ng, or about 1100 ng, or about 1200 ng, or about 1300 ng, or about 1400 ng, or about 1500 ng, or about 1600 ng, or about 1700 ng, or about 1800 ng, or about 1900 ng, or about 2000 ng, or about 3000 ng, or about 4000 ng, or about 5000 ng.

Some embodiments are directed to synthetic RNA molecules with low toxicity and high translation efficiency. Other embodiments are directed to a cell-culture medium for high-efficiency in vivo transfection, reprogramming, and gene editing of cells. Other embodiments pertain to methods for producing synthetic RNA molecules encoding reprogramming proteins. Still further embodiments pertain to methods for producing synthetic RNA molecules encoding gene-editing proteins.

Some embodiments are directed to methods of gene-editing and/or gene correction. Some embodiments encompass synthetic RNA-based gene-editing and/or gene correction, e.g. with RNA comprising non-canonical nucleotides, e.g. RNA encoding one or more of a nuclease, a transcription activator-like effector nuclease (TALEN), a zinc-finger nuclease, a meganuclease, a nickase, a clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein a DNA-repair protein, a DNA-modification protein, a base-modification protein, a DNA methyltransferase, an protein that causes DNA demethylation, an enzyme for which DNA is a substrate or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof. In some embodiments, the efficiency of the gene-editing and/or gene correction is high, for example, higher than DNA-based gene editing and/or gene correction. In some embodiments, the present methods of gene-editing and/or gene correction are efficient enough for in vivo application. In some embodiments, the present methods of gene-editing and/or gene correction are efficient enough to not require cellular selection (e.g. selection of cells that have been edited). In various embodiments, the efficiency of gene-editing of the present methods is about 1%, or about 2%, or about 3%, or about 4%, or about 5%, or about 6%, or about 7%, or about 8%, or about 9%, or about 10%, or about 20%, or about 30%, or about 40%, or about 50%, or about 60%, or about 70%, or about 80%, or about 90%, or about 100%. In various embodiments, the efficiency of gene-correction of the present methods is about 1%, or about 2%, or about 3%, or about 4%, or about 5%, or about 6%, or about 7%, or about 8%, or about 9%, or about 10%, or about 20%, or about 30%, or about 40%, or about 50%, or about 60%, or about 70%, or about 80%, or about 90%, or about 100%

Some embodiments are directed to high-efficiency gene-editing proteins comprising engineered nuclease cleavage or DNA-modification domains. Other embodiments are directed to high-fidelity gene-editing proteins comprising engineered nuclease cleavage or DNA-modification domains. Various embodiments are directed to high-efficiency gene-editing proteins comprising engineered DNA-binding domains. Other embodiments are directed to high-fidelity gene-editing proteins comprising engineered DNA-binding domains. Still other embodiments are directed to gene-editing proteins comprising engineered repeat sequences. Some embodiments are directed to gene-editing proteins comprising one or more CRISPR associated family members. Some embodiments are directed to methods for altering the DNA sequence of a cell by transfecting the cell with or inducing the cell to express a gene-editing protein. Other embodiments are directed to methods for altering the DNA sequence of a cell that is present in an in vitro culture. Still further embodiments are directed to methods for altering the DNA sequence of a cell that is present in vivo.

Some embodiments are directed to methods of modulating the secretion or subcellular localization of a polypeptide. In such embodiments, the present invention provides an RNA encoding a protein comprising a signal peptide operably linked to a polypeptide having biological functionality. In an embodiment, the signal peptide modulates the secretion of the polypeptide. In another embodiment, the signal peptide modulates the subcellular localization of the polypeptide. For example, BMP7 comprising at least one FGF21 signal peptide may result in increased secretion of BMP7. In another example, BMP7 comprising at least one FGF21 signal peptide in place of the endogenous BMP7 signal peptide may result in increased secretion of BMP7. In various embodiments, any of the proteins listed in Table 2A or Table 2B comprising at least one signal peptide listed in the table below may result in increased secretion of the proteins.

SEQ

ID

Protein
Signal Peptide
NO

Gaussia
MGVKVLFALI
596

luciferase
CIAVAEA

Human BMP7
MHVRSLRAAA
597

PHSFVALWAP

LFLLRSALA

Human
MAFLWLLSCW
598

chymotrypsinogen B
ALLGTTFG

Human
MLGITVLAAL
599

chymotrypsinogen C
LACASS

Human EPO
MGVHECPAWL
600

WLLLSLLSLP

LGLPVLG

Human FGF19
MRSGCVVVHV
601

WILAGLWLAV

AGRP

Human FGF21
MDSDETGFEH
602

SGLWVSVLAG

LLLGACQA

Human FGF23
MLGARLRLWV
603

CALCSVCSMS

VLRA

Human IL2
MYRMQLLSCI
604

ALSLALVTNS

Human IL22
MAALQKSVSS
605

FLMGTLATSC

LLLLALLVQG

GAA

Human IL6
MNSFSTSAFG
606

PVAFSLGLLL

VLPAAFPAP

Human
MALTFALLVA
607

Interferon
LLVLSCKSSC

Alpha-2
SVG

Human
MTNKCLLQIA
608

Interferon
LLLCFSTTAL

Beta
S

Human
MKYTSYILAF
609

Interferon
QLCIVLGSLG

Gamma
CYC

Human
MNPLLILTFV
610

Trypsin-1
AAALA

Some embodiments are directed to methods of modulating the serum half-life, secretion, bioavailability, and/or activity of a protein comprising administering a RNA encoding the protein and a RNA encoding a receptor for the protein. For example, IL15 may be administered with its receptor, e.g., IL15RA to enhance one or more of the serum half-life, secretion, bioavailability, and activity of IL15.

Many proteins and peptides, when translated from in vitro transcribed RNA, can exhibit reduced activity resulting from incomplete or inadequate post-translational processing. It has now been discovered that the amount of active protein, polypeptide or peptide produced following RNA transfection can be increased by contacting cells with and/or inducing cells to express a second protein that is capable of processing a polypeptide into the protein, peptide or polypeptide. Certain embodiments are therefore directed to a method for inducing a cell to express an active protein, polypeptide or peptide comprising contacting the cell with a synthetic RNA molecule encoding a first polypeptide and contacting the cell with a second protein that is capable of processing the first polypeptide into an active protein, polypeptide or peptide.

In certain embodiments, the second protein is administered to a patient. In one embodiment, the second protein is a recombinant protein. In another embodiment, the cells are contacted with a synthetic RNA molecule encoding the second protein. In a further embodiment, the cells are contacted with and/or induced to express an inhibitor of a molecule that inhibits the second protein. In one embodiment, the inhibitor is a short interfering RNA molecule.

In certain embodiments, the second protein is a member of the PCSK family. In other embodiments, the second protein is a proprotein convertase. In still other embodiments, the second protein is a prohormone convertase. In still other embodiments, the second protein is a carboxypeptidase. In one embodiment, the second protein is PCSK3 (Furin/PACE). In another embodiment, the second protein is primarily secreted. In yet another embodiment, the second protein is primarily intracellular. In a further embodiment, the first polypeptide, protein, polypeptide or peptide is selected from the group: a product of proopiomelanocortin, renin, a product of enkephalin, a product of prodynorphin, somatostatin, insulin, agouti-related peptide, glucagon, parathyroid hormone, a member of the transforming growth factor beta superfamily, albumin, beta-secretase 1, nerve growth factor, caldesmon, the alpha-integrins, factor IX, α-melanocyte-stimulating hormone, adrenocorticotropic hormone, β-endorphin, and met-enkefalin.

Glycation and glycosylation are processes by which one or more sugar molecules are bound to a protein. It has now been discovered that altering the number or location of glycation and glycosylation sites can increase or decrease the stability of a protein. Certain embodiments are therefore directed to a protein with one or more glycation or glycosylation sites. In one embodiment, the protein is engineered to have more glycation or glycosylation sites than a natural variant of the protein. In another embodiment, the protein is engineered to have fewer glycation or glycosylation sites than a natural variant of the protein. In yet another embodiment, the protein has increased stability. In yet another embodiment, the protein has decreased stability. In some embodiments, the protein is a circulating protein. In one embodiment, the protein is erythropoietin or a biologically active fragment, variant, analogue, or family-member thereof. In another embodiment, the protein is darbepoetin alfa or a biologically active fragment, variant, analogue, or family-member thereof. In another embodiment, the protein is NOVEPOETIN or a biologically active fragment, variant, analogue, or family-member thereof.

It has been further discovered that in certain situations, including one or more steroids and/or one or more antioxidants in the transfection medium can increase in vivo transfection efficiency, in vivo reprogramming efficiency, and in vivo gene-editing efficiency. Certain embodiments are therefore directed to contacting a cell or patient with a glucocorticoid, such as hydrocortisone, prednisone, prednisolone, methylprednisolone, dexamethasone or betamethasone. Other embodiments are directed to a method for inducing a cell to express a protein of interest by contacting a cell with a medium containing a steroid and contacting the cell with one or more nucleic acid molecules. In one embodiment, the nucleic acid molecule comprises synthetic RNA. In another embodiment, the steroid is hydrocortisone. In yet another embodiment, the hydrocortisone is present in the medium at a concentration of between about 0.1 uM and about 10 uM, or about 1 uM. Other embodiments are directed to a method for inducing a cell in vivo to express a protein of interest by contacting the cell with a medium containing an antioxidant and contacting the cell with one or more nucleic acid molecules. In one embodiment, the antioxidant is ascorbic acid or ascorbic-acid-2-phosphate. In another embodiment, the ascorbic acid or ascorbic-acid-2-phosphate is present in the medium at a concentration of between about 0.5 mg/L and about 500 mg/L, including about 50 mg/L. Still other embodiments are directed to a method for reprogramming and/or gene-editing a cell in vivo by contacting the cell with a medium containing a steroid and/or an antioxidant and contacting the cell with one or more nucleic acid molecules, wherein the one or more nucleic acid molecules encodes one or more reprogramming and/or gene-editing proteins. In certain embodiments, the cell is present in an organism, and the steroid and/or antioxidant are delivered to the organism.

Adding transferrin to the complexation medium has been reported to increase the efficiency of plasmid transfection in certain situations. It has now been discovered that adding transferrin to the complexation medium can also increase the efficiency of in vivo transfection with synthetic RNA molecules. Certain embodiments are therefore directed to a method for inducing a cell in vivo to express a protein of interest by adding one or more synthetic RNA molecules and a transfection reagent to a solution containing transferrin. In one embodiment, the transferrin is present in the solution at a concentration of between about 1 mg/L and about 100 mg/L, such as about 5 mg/L. In another embodiment, the transferrin is recombinant.

In certain situations, including pertaining to culturing, it may be desirable to replace animal-derived components with non-animal-derived and/or recombinant components, in part because non-animal-derived and/or recombinant components can be produced with a higher degree of consistency than animal-derived components, and in part because non-animal-derived and/or recombinant components carry less risk of contamination with toxic and/or pathogenic substances than do animal-derived components. Certain embodiments are therefore directed to a protein that is non-animal-derived and/or recombinant. Other embodiments are directed to a medium, wherein some or all of the components of the medium are non-animal-derived and/or recombinant.

Other embodiments are directed to a method for transfecting a cell in vivo. In one embodiment, a cell in vivo is transfected with one or more nucleic acids, and the transfection is performed using a transfection reagent, such as a lipid-based transfection reagent. In one embodiment, the one or more nucleic acids includes at least one RNA molecule. In another embodiment, the cell is transfected with one or more nucleic acids, and the one or more nucleic acids encodes at least one of: p53, TERT, an antibody, an extracellular matrix protein, a cytokine, a secreted protein, a membrane-bound protein, an enzyme, a gene-editing protein, a chromatin-modifying protein, a DNA-binding protein, a transcription factor, a histone deacetylase, a pathogen-associated molecular pattern, and a tumor-associated antigen or a biologically active fragment, analogue, variant or family-member thereof. In another embodiment, the cell is transfected repeatedly, such as at least about 2 times during about 10 consecutive days, or at least about 3 times during about 7 consecutive days, or at least about 4 times during about 6 consecutive days. Some embodiments are directed to a method for increasing expression of telomerase in one of a fibroblast, a hematopoietic stem cell, a mesenchymal stem cells, a cardiac stem cell, a hair follicle stem cell, a neural stem cell, an intestinal stem cell, an endothelial stem cell, an olfactory stem cell, a neural crest stem cell, a testicular cell, and a keratinocyte. Some embodiments are directed to a method for increasing the length of telomeres in one of a fibroblast, a hematopoietic stem cell, a mesenchymal stem cells, a cardiac stem cell, a hair follicle stem cell, a neural stem cell, an intestinal stem cell, an endothelial stem cell, an olfactory stem cell, a neural crest stem cell, a testicular cell, and a keratinocyte. Other embodiments are directed to a method for isolating a cell from a patient, contacting the cell with a nucleic acid drug encoding a component of telomerase (e.g., TERT), and reintroducing the cell to the patient. Various embodiments are directed to a method for increasing the replicative potential of a cell.

Reprogramming can be performed by transfecting cells with one or more nucleic acids encoding one or more reprogramming factors. Examples of reprogramming factors include, but are not limited to: Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, I-Myc protein, TERT protein, Nanog protein, Lin28 protein, Utf1 protein, Aicda protein, miR200 micro-RNA, miR302 micro-RNA, miR367 micro-RNA, miR369 micro-RNA and biologically active fragments, analogues, variants and family-members thereof. Certain embodiments are therefore directed to a method for reprogramming a cell in vivo. In one embodiment, the cell in vivo is reprogrammed by transfecting the cell with one or more nucleic acids encoding one or more reprogramming factors. In one embodiment, the one or more nucleic acids includes an RNA molecule that encodes Oct4 protein. In another embodiment, the one or more nucleic acids also includes one or more RNA molecules that encodes Sox2 protein, Klf4 protein, and c-Myc protein. In yet another embodiment, the one or more nucleic acids also includes an RNA molecule that encodes Lin28 protein. In one embodiment, the cell is a human skin cell, and the human skin cell is reprogrammed to a pluripotent stem cell. In another embodiment, the cell is a human skin cell, and the human skin cell is reprogrammed to a glucose-responsive insulin-producing cell. Examples of other cells that can be reprogrammed and other cells to which a cell can be reprogrammed include, but are not limited to: skin cells, pluripotent stem cells, mesenchymal stem cells, β-cells, retinal pigmented epithelial cells, hematopoietic cells, cardiac cells, airway epithelial cells, neural stem cells, neurons, glial cells, bone cells, blood cells, and dental pulp stem cells. In one embodiment, the cell is contacted with a medium that supports the reprogrammed cell. In one embodiment, the medium also supports the cell.

Importantly, infecting skin cells with viruses encoding Oct4, Sox2, Klf4, and c-Myc, combined with culturing the cells in a medium that supports the growth of cardiomyocytes, has been reported to cause reprogramming of the skin cells to cardiomyocytes, without first reprogramming the skin cells to pluripotent stem cells (See Efs et al Nat Cell Biol. 2011; 13:215-22, the contents of which are hereby incorporated by reference). In certain situations, direct reprogramming (reprogramming one somatic cell to another somatic cell without first reprogramming the somatic cell to a pluripotent stem cell, also known as “transdifferentiation”) may be desirable, in part because culturing pluripotent stem cells can be time-consuming and expensive, the additional handling involved in establishing and characterizing a stable pluripotent stem cell line can carry an increased risk of contamination, and the additional time in culture associated with first producing pluripotent stem cells can carry an increased risk of genomic instability and the acquisition of mutations, including point mutations, copy-number variations, and karyotypic abnormalities. Certain embodiments are therefore directed to a method for reprogramming a somatic cell in vivo, wherein the cell is reprogrammed to a somatic cell, and wherein a characterized pluripotent stem-cell line is not produced.

It has been further discovered that, in certain situations, fewer total transfections may be required to reprogram a cell according to the methods of the present invention than according to other methods. Certain embodiments are therefore directed to a method for reprogramming a cell in vivo, wherein between about 1 and about 12 transfections are performed during about 20 consecutive days, or between about 4 and about 10 transfections are performed during about 15 consecutive days, or between about 4 and about 8 transfections are performed during about 10 consecutive days. It is recognized that when a cell is contacted with a medium containing nucleic acid molecules, the cell may likely come into contact with and/or internalize more than one nucleic acid molecule either simultaneously or at different times. A cell can therefore be contacted with a nucleic acid more than once, e.g. repeatedly, even when a cell is contacted only once with a medium containing nucleic acids.

Of note, nucleic acids can contain one or more non-canonical or “modified” residues as described herein. For instance, any of the non-canonical nucleotides described herein can be used in the present reprgramming methods. In one embodiment, pseudouridine-5′-triphosphate can be substituted for uridine-5′-triphosphate in an in vitro-transcription reaction to yield synthetic RNA, wherein up to 100% of the uridine residues of the synthetic RNA may be replaced with pseudouridine residues. In vitro-transcription can yield RNA with residual immunogenicity, even when pseudouridine and 5-methylcytidine are completely substituted for uridine and cytidine, respectively (see, e.g., Angel. Reprogramming Human Somatic Cells to Pluripotency Using RNA [Doctoral Thesis]. Cambridge, Mass.: MIT; 2011, the contents of which are hereby incorporated by reference). For this reason, it is common to add an immunosuppressant to the transfection medium when transfecting cells with RNA. In certain situations, adding an immunosuppressant to the transfection medium may not be desirable, in part because the recombinant immunosuppressant most commonly used for this purpose, B18R, can be expensive and difficult to manufacture. It has now been discovered that cells in vivo can be transfected and/or reprogrammed according to the methods of the present invention, without using B18R or any other immunosuppressant. It has been further discovered that reprogramming cells in vivo according to the methods of the present invention without using immunosuppressants can be rapid, efficient, and reliable. Certain embodiments are therefore directed to a method for transfecting a cell in vivo, wherein the transfection medium does not contain an immunosuppressant. Other embodiments are directed to a method for reprogramming a cell in vivo, wherein the transfection medium does not contain an immunosuppressant. In certain situations, for example when using a high cell density, it may be beneficial to add an immunosuppressant to the transfection medium. Certain embodiments are therefore directed to a method for transfecting a cell in vivo, wherein the transfection medium contains an immunosuppressant. Other embodiments are directed to a method for reprogramming a cell in vivo, wherein the transfection medium contains an immunosuppressant. In one embodiment, the immunosuppressant is B18R or a biologically active fragment, analogue, variant or family-member thereof or dexamethasone or a derivative thereof. In one embodiment, the transfection medium does not contain an immunosuppressant, and the nucleic-acid dose is chosen to prevent excessive toxicity. In another embodiment, the nucleic-acid dose is less than about 1 mg/cm²of tissue or less than about 1 mg/100,000 cells or less than about 10 mg/kg.

Reprogrammed cells produced according to certain embodiments of the present invention are suitable for therapeutic and/or cosmetic applications as they do not contain undesirable exogenous DNA sequences, and they are not exposed to animal-derived or human-derived products, which may be undefined, and which may contain toxic and/or pathogenic contaminants. Furthermore, the high speed, efficiency, and reliability of certain embodiments of the present invention may reduce the risk of acquisition and accumulation of mutations and other chromosomal abnormalities. Certain embodiments of the present invention can thus be used to generate cells that have a safety profile adequate for use in therapeutic and/or cosmetic applications. For example, reprogramming cells using RNA and the medium of the present invention, wherein the medium does not contain animal or human-derived components, can yield cells that have not been exposed to allogeneic material. Certain embodiments are therefore directed to a reprogrammed cell that has a desirable safety profile. In one embodiment, the reprogrammed cell has a normal karyotype. In another embodiment, the reprogrammed cell has fewer than about 5 copy-number variations (CNVs) relative to the patient genome, such as fewer than about 3 copy-number variations relative to the patient genome, or no copy-number variations relative to the patient genome. In yet another embodiment, the reprogrammed cell has a normal karyotype and fewer than about 100 single nucleotide variants in coding regions relative to the patient genome, or fewer than about 50 single nucleotide variants in coding regions relative to the patient genome, or fewer than about 10 single nucleotide variants in coding regions relative to the patient genome.

Endotoxins and nucleases can co-purify and/or become associated with other proteins, such as serum albumin. Recombinant proteins, in particular, can often have high levels of associated endotoxins and nucleases, due in part to the lysis of cells that can take place during their production. Endotoxins and nucleases can be reduced, removed, replaced or otherwise inactivated by many of the methods of the present invention, including, for example, by acetylation, by addition of a stabilizer such as sodium octanoate, followed by heat treatment, by the addition of nuclease inhibitors to the albumin solution and/or medium, by crystallization, by contacting with one or more ion-exchange resins, by contacting with charcoal, by preparative electrophoresis or by affinity chromatography. It has now been discovered that partially or completely reducing, removing, replacing or otherwise inactivating endotoxins and/or nucleases from a medium and/or from one or more components of a medium can increase the efficiency with which cells can be transfected and reprogrammed. Certain embodiments are therefore directed to a method for transfecting a cell in vivo with one or more nucleic acids, wherein the transfection medium is treated to partially or completely reduce, remove, replace or otherwise inactivate one or more endotoxins and/or nucleases. Other embodiments are directed to a medium that causes minimal degradation of nucleic acids. In one embodiment, the medium contains less than about 1EU/mL, or less than about 0.1EU/mL, or less than about 0.01EU/mL.

In certain situations, protein-based lipid carriers such as serum albumin can be replaced with non-protein-based lipid carriers such as methyl-beta-cyclodextrin. The medium of the present invention can also be used without a lipid carrier, for example, when transfection is performed using a method that may not require or may not benefit from the presence of a lipid carrier, for example, using one or more lipid-based transfection reagents, polymer-based transfection reagents or peptide-based transfection reagents or using electroporation. Many protein-associated molecules, such as metals, can be highly toxic to cells in vivo. This toxicity can cause decreased viability, as well as the acquisition of mutations. Certain embodiments thus have the additional benefit of producing cells that are free from toxic molecules.

The associated-molecule component of a protein can be measured by suspending the protein in solution and measuring the conductivity of the solution. Certain embodiments are therefore directed to a medium that contains a protein, wherein about a 10% solution of the protein in water has a conductivity of less than about 500 μmho/cm. In one embodiment, the solution has a conductivity of less than about 50 μmho/cm. In another embodiment, less than about 0.65% of the dry weight of the protein comprises lipids and/or less than about 0.35% of the dry weight of the protein comprises free fatty acids.

The amount of nucleic acid delivered to cells in vivo can be increased to increase the desired effect of the nucleic acid. However, increasing the amount of nucleic acid delivered to cells in vivo beyond a certain point can cause a decrease in the viability of the cells, due in part to toxicity of the transfection reagent. It has now been discovered that when a nucleic acid is delivered to a population of cells in vivo in a fixed volume (for example, cells in a region of tissue), the amount of nucleic acid delivered to each cell can depend on the total amount of nucleic acid delivered to the population of cells and to the density of the cells, with a higher cell density resulting in less nucleic acid being delivered to each cell. In certain embodiments, a cell in vivo is transfected with one or more nucleic acids more than once. Under certain conditions, for example when the cells are proliferating, the cell density may change from one transfection to the next. Certain embodiments are therefore directed to a method for transfecting a cell in vivo with a nucleic acid, wherein the cell is transfected more than once, and wherein the amount of nucleic acid delivered to the cell is different for two of the transfections. In one embodiment, the cell proliferates between two of the transfections, and the amount of nucleic acid delivered to the cell is greater for the second of the two transfections than for the first of the two transfections. In another embodiment, the cell is transfected more than twice, and the amount of nucleic acid delivered to the cell is greater for the second of three transfections than for the first of the same three transfections, and the amount of nucleic acid delivered to the cells is greater for the third of the same three transfections than for the second of the same three transfections. In yet another embodiment, the cell is transfected more than once, and the maximum amount of nucleic acid delivered to the cell during each transfection is sufficiently low to yield at least about 80% viability for at least two consecutive transfections.

It has now been discovered that modulating the amount of nucleic acid delivered to a population of proliferating cells in vivo in a series of transfections can result in both an increased effect of the nucleic acid and increased viability of the cells. It has been further discovered that, in certain situations, when cells in vivo are contacted with one or more nucleic acids encoding one or more reprogramming factors in a series of transfections, the efficiency of reprogramming can be increased when the amount of nucleic acid delivered in later transfections is greater than the amount of nucleic acid delivered in earlier transfections, for at least part of the series of transfections. Certain embodiments are therefore directed to a method for reprogramming a cell in vivo, wherein one or more nucleic acids is repeatedly delivered to the cell in a series of transfections, and the amount of the nucleic acid delivered to the cell is greater for at least one later transfection than for at least one earlier transfection. In one embodiment, the cell is transfected between about 2 and about 10 times, or between about 3 and about 8 times, or between about 4 and about 6 times. In another embodiment, the one or more nucleic acids includes at least one RNA molecule, the cell is transfected between about 2 and about 10 times, and the amount of nucleic acid delivered to the cell in each transfection is the same as or greater than the amount of nucleic acid delivered to the cell in the most recent previous transfection. In yet another embodiment, the amount of nucleic acid delivered to the cell in the first transfection is between about 20 ng/cm²and about 250 ng/cm², or between 100 ng/cm²and 600 ng/cm². In yet another embodiment, the cell is transfected about 5 times at intervals of between about 12 and about 48 hours, and the amount of nucleic acid delivered to the cell is about 25 ng/cm²for the first transfection, about 50 ng/cm²for the second transfection, about 100 ng/cm²for the third transfection, about 200 ng/cm²for the fourth transfection, and about 400 ng/cm²for the fifth transfection. In yet another embodiment, the cell is further transfected at least once after the fifth transfection, and the amount of nucleic acid delivered to the cell is about 400 ng/cm².

Certain embodiments are directed to a method for transfecting a cell in vivo with a nucleic acid, wherein the amount of nucleic acid is determined by measuring the cell density, and choosing the amount of nucleic acid to transfect based on the measurement of cell density. In one embodiment, the cell density is measured by optical means. In another embodiment, the cell is transfected repeatedly, the cell density increases between two transfections, and the amount of nucleic acid transfected is greater for the second of the two transfections than for the first of the two transfections.

It has now been discovered that, in certain situations, the amount of a circulating protein that is produced in a patient can be increased by administering to a patient a nucleic acid at a plurality of administration sites. In certain embodiments, the amount of a circulating protein is increased relative to the amount of the circulating protein that is produced in a patient by administering to the patient the nucleic acid at a single injection site. In one embodiment, the administering is by injection. In another embodiment, the injection is intradermal injection. In still another embodiment, the injection is subcutaneous or intramuscular injection. In some embodiments, the plurality of administration sites comprise administration sites in the skin. In other embodiments, the plurality of administration sites are at least about 1 or at least about 2 or at least about 5 or at least about 10 or at least about 20 or at least about 50 or at least about 100 administration sites. In one embodiment, the administering is performed within at least about 5 minutes or at least about 10 minutes or at least about 30 minutes or at least about 1 hour or at least about 2 hours or at least about 5 hours or at least about 12 hours or at least about 1 day. In certain embodiments, the amount of a circulating protein is increased by at least about 10 percent or at least about 20 percent or at least about 50 percent or at least about 100 percent or at least about 3-fold or at least about 5-fold or at least about 10-fold or at least about 20-fold or at least about 50-fold or at least about 100-fold or at least about 500-fold or at least about 1000-fold or greater than 1000-fold.

It has now been discovered that, in certain situations, the in vivo transfection efficiency and viability of cells contacted with the medium of the present invention can be improved by conditioning the medium. Certain embodiments are therefore directed to a method for conditioning a medium. Other embodiments are directed to a medium that is conditioned. In one embodiment, the feeders are fibroblasts, and the medium is conditioned for approximately 24 hours. Other embodiments are directed to a method for transfecting a cell in vivo, wherein the transfection medium is conditioned. Other embodiments are directed to a method for reprogramming and/or gene-editing a cell in vivo, wherein the medium is conditioned. In one embodiment, the feeders are mitotically inactivated, for example, by exposure to a chemical such as mitomycin-C or by exposure to gamma radiation. In certain embodiments, it may be beneficial to use only autologous materials, in part to, for example and not wishing to be bound by theory, avoid the risk of disease transmission from the feeders to the cell or the patient. Certain embodiments are therefore directed to a method for transfecting a cell in vivo, wherein the transfection medium is conditioned, and wherein the feeders are derived from the same individual as the cell being transfected. Other embodiments are directed to a method for reprogramming and/or gene-editing a cell in vivo, wherein the medium is conditioned, and wherein the feeders are derived from the same individual as the cell being reprogrammed and/or gene-edited.

Several molecules can be added to media by conditioning. Certain embodiments are therefore directed to a medium that is supplemented with one or more molecules that are present in a conditioned medium. In one embodiment, the medium is supplemented with Wnt1, Wnt2, Wnt3, Wnt3a or a biologically active fragment, analogue, variant, agonist, or family-member thereof. In another embodiment, the medium is supplemented with TGF-β or a biologically active fragment, analogue, variant, agonist, or family-member thereof. In yet another embodiment, a cell in vivo is reprogrammed according to the method of the present invention, wherein the medium is not supplemented with TGF-β for between about 1 and about 5 days, and is then supplemented with TGF-β for at least about 2 days. In yet another embodiment, the medium is supplemented with IL-6, IL-6R or a biologically active fragment, analogue, variant, agonist, or family-member thereof. In yet another embodiment, the medium is supplemented with a sphingolipid or a fatty acid. In still another embodiment, the sphingolipid is lysophosphatidic acid, lysosphingomyelin, sphingosine-1-phosphate or a biologically active analogue, variant or derivative thereof.

In addition to mitotically inactivating cells, under certain conditions, irradiation can change the gene expression of cells, causing cells to produce less of certain proteins and more of certain other proteins that non-irradiated cells, for example, members of the Wnt family of proteins. In addition, certain members of the Wnt family of proteins can promote the growth and transformation of cells. It has now been discovered that, in certain situations, the efficiency of reprogramming can be greatly increased by contacting a cell in vivo with a medium that is conditioned using irradiated feeders instead of mitomycin-c-treated feeders. It has been further discovered that the increase in reprogramming efficiency observed when using irradiated feeders is caused in part by Wnt proteins that are secreted by the feeders. Certain embodiments are therefore directed to a method for reprogramming a cell in vivo, wherein the cell is contacted with Wnt1, Wnt2, Wnt3, Wnt3a or a biologically active fragment, analogue, variant, family-member or agonist thereof, including agonists of downstream targets of Wnt proteins, and/or agents that mimic one or more of the biological effects of Wnt proteins, for example, 2-amino-4-[3,4-(methylenedioxy)benzylamino]-6-(3-methoxyphenyl)pyrimidine.

Because of the low efficiency of many DNA-based reprogramming methods, these methods may be difficult or impossible to use with cells derived from patient samples, which may contain only a small number of cells. In contrast, the high efficiency of certain embodiments of the present invention can allow reliable reprogramming of a small number of cells, including single cells. Certain embodiments are directed to a method for reprogramming a small number of cells. Other embodiments are directed to a method for reprogramming a single cell. In one embodiment, the cell is contacted with one or more enzymes. In another embodiment, the enzyme is collagenase. In yet another embodiment, the collagenase is animal-component free. In one embodiment, the collagenase is present at a concentration of between about 0.1 mg/mL and about 10 mg/mL, or between about 0.5 mg/mL and about 5 mg/mL. In another embodiment, the cell is a blood cell. In yet another embodiment, the cell is contacted with a medium containing one or more proteins that is derived from the patient's blood. In still another embodiment, the cell is contacted with a medium comprising: DMEM/F12+2 mM L-alanyl-L-glutamine+between about 5% and about 25% patient-derived serum, or between about 10% and about 20% patient-derived serum, or about 20% patient-derived serum.

It has now been discovered that, in certain situations, transfecting cells in vivo with a mixture of RNA encoding Oct4, Sox2, Klf4, and c-Myc using the medium of the present invention can cause the rate of proliferation of the cells to increase. When the amount of RNA delivered to the cells is too low to ensure that all of the cells are transfected, only a fraction of the cells may show an increased proliferation rate. In certain situations, such as when generating a personalized therapeutic, increasing the proliferation rate of cells may be desirable, in part because doing so can reduce the time necessary to generate the therapeutic, and therefore can reduce the cost of the therapeutic. Certain embodiments are therefore directed to a method for transfecting a cell in vivo with a mixture of RNA encoding Oct4, Sox2, Klf4, and c-Myc. In one embodiment, the cell exhibits an increased proliferation rate. In another embodiment, the cell is reprogrammed.

Many diseases are associated with one or more mutations. Mutations can be corrected by contacting a cell with a nucleic acid that encodes a protein that, either alone or in combination with other molecules, corrects the mutation (an example of gene-editing). Examples of such proteins include: a nuclease, a transcription activator-like effector nuclease (TALEN), a zinc-finger nuclease, a meganuclease, a nickase, a clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein a DNA-repair protein, a DNA-modification protein, a base-modification protein, a DNA methyltransferase, an protein that causes DNA demethylation, an enzyme for which DNA is a substrate or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof. Certain embodiments are therefore directed to a method for transfecting a cell in vivo with a nucleic acid, wherein the nucleic acid encodes a protein that, either alone or in combination with other molecules, creates a single-strand or double-strand break in a DNA molecule. In a one embodiment, the protein is a zinc finger nuclease or a TALEN. In another embodiment, the nucleic acid is an RNA molecule. In yet another embodiment, the single-strand or double-strand break is within about 5,000,000 bases of the transcription start site of a gene selected from the group: SERPINA1, CCR5, CXCR4, GAD1, GAD2, CFTR, HBA1, HBA2, HBB, HBD, FANCA, XPA, XPB, XPC, ERCC2, POLH, HTT, DMD, SOD1, APOE, PRNP, BRCA1, and BRCA2 or an analogue, variant or family-member thereof. In one embodiment, the present invention relates to gene-editing of the MYC protein (e.g. correcting one or more muations that may be linked to cancer), optionally with a TALEN. In yet another embodiment, the cell is transfected with a nucleic acid that acts as a repair template by either causing the insertion of a DNA sequence in the region of the single-strand or double-strand break or by causing the DNA sequence in the region of the single-strand or double-strand break to otherwise change. In yet another embodiment, the gene-editing protein contains a DNA modification domain. In yet another embodiment, the gene-editing protein corrects a mutation without creating a single-strand break. In yet another embodiment, the gene-editing protein corrects a mutation without creating a double-strand break. In yet another embodiment, the gene-editing protein corrects a mutation by causing the replacement of one base with another base. In one embodiment, adenine is replaced by cytosine. In another embodiment, adenine is replaced by guanine. In yet another embodiment, adenine is replaced by thymine. In yet another embodiment, cytosine is replaced by adenine. In yet another embodiment, cytosine is replaced by guanine. In yet another embodiment, cytosine is replaced by thymine. In yet another embodiment, guanine is replaced by adenine. In yet another embodiment, guanine is replaced by cytosine. In yet another embodiment, guanine is replaced by thymine. In yet another embodiment, thymine is replaced by adenine. In yet another embodiment, thymine is replaced by cytosine. In yet another embodiment, thymine is replaced by guanine. In one embodiment, the replacement of one base with another base is a one-step process. In another embodiment, the replacement of one base with another base is a multi-step process. In some embodiments, one base is replaced by more than one base, for example, by two bases. In other embodiments, more than one base is replaced by one base. In still other embodiments, more than one base is replaced by more than one base. In some embodiments, the gene-editing protein contains a deaminase domain. In one embodiment the deaminase domain comprises a cytidine deaminase domain. In another embodiment, the deaminase domain comprises an adenosine deaminase domain. In yet another embodiment, the deaminase domain comprises a guanosine deaminase domain. In yet another embodiment, the gene-editing protein comprises a sequence that is at least about 50% or at least about 60% or at least about 70% or at least about 80% or at least about 90% or at least about 95% or at least about 99% homologous to one or more of: SEQ ID NOs: 587, 588, 589, 590, 591, 592, and 593. In yet another embodiment, the gene-editing protein comprises a linker. In one embodiment, the linker is a flexible linker. In another embodiment, the linker positions the deaminase domain in proximity to a target base. In yet another embodiment, the gene-editing protein deaminates the target base. In yet another embodiment, the gene-editing protein comprises a glycosylase-inhibitor domain. In yet another embodiment, the gene-editing protein comprises glycosylase-inhibitor activity. In one embodiment, the glycosylase inhibitor is a uracil glycosylase inhibitor. In another embodiment, the glycosylase inhibitor is a N-methylpurine DNA glycosylase inhibitor. In yet another embodiment, the cell is reprogrammed, and subsequently, the cell is gene-edited. In yet another embodiment, the cell is gene-edited, and subsequently, the cell is reprogrammed. In yet another embodiment, the gene-editing and reprogramming are performed within about 7 days of each other. In yet another embodiment, the gene-editing and reprogramming occur simultaneously or on the same day. In yet another embodiment, the cell is a skin cell, the skin cell is gene-edited to disrupt the CCR5 gene, the skin cell is reprogrammed to a hematopoietic stem cell, thus producing a therapeutic for HIV/AIDS, and the therapeutic is used to treat a patient with HIV/AIDS. In yet another embodiment, the skin cell is derived from the same patient whom the therapeutic is used to treat.

Certain embodiments are directed to methods and compositions for the treatment of rare diseases. In some embodiments, the rare disease is one or more of: a rare metabolic disease, a rare cardiovascular disease, a rare dermatologic disease, a rare neurologic disease, a rare developmental disease, a rare genetic disease, a rare pulmonary disease, a rare liver disease, a rare kidney disease, a rare psychiatric disease, a rare reproductive disease, a rare musculoskeletal disease, a rare orthopedic disease, an inborn error of metabolism, a lysosomal storage disease, and a rare ophthalmologic disease. In one embodiment, the disease is alpha-1-antitrypsin deficiency. Some embodiments are directed to a treatment comprising a nucleic acid encoding a gene-editing protein that is capable of causing a deletion in a gene that is associated with one or more of: a gain-of-function mutation, a loss-of-function mutation, a recessive mutation, a dominant mutation or a dominant negative mutation. Other embodiments are directed to a treatment comprising a nucleic acid encoding a gene-editing protein that is capable of correcting one or more of: a gain-of-function mutation, a loss-of-function mutation, a recessive mutation, a dominant mutation or a dominant negative mutation. In one embodiment, the treatment ameliorates one or more of the symptoms in a subject. In another embodiment, the subject is a human subject. In yet another embodiment, the subject is a veterinary subject. Some embodiments are directed to a treatment for alpha-1-antitrypsin deficiency comprising administering to a subject a nucleic acid comprising a gene-editing protein that is capable of causing a deletion in or near the SERPINA1 gene. Other embodiments are directed to a treatment for alpha-1-antitrypsin deficiency comprising administering to a subject a nucleic acid encoding a gene-editing protein that is capable of correcting a mutation in or near the SERPINA1 gene. In one embodiment the mutation is the Z mutation. In one embodiment, the deletion or correction reduces the accumulation of polymerized alpha-1-antitrypsin protein in the subject's cells and/or increases the secretion of alpha-1-antitrypsin from the subject's cells. In another embodiment, the treated cells regenerate a diseased organ. In yet another embodiment, the diseased organ is the liver. In yet another embodiment, the diseased organ is the lung. In yet another embodiment, the treatment delays or eliminates the subject's need for a liver and/or lung transplant. Other embodiments are directed to a treatment for epidermolysis bullosa. In one embodiment the epidermolysis bullosa is dystrophic epidermolysis bullosa. In another embodiment, the epidermolysis bullosa is epidermolysis bullosa simplex. In yet another embodiment, the dystrophic epidermolysis bullosa is recessive dystrophic epidermolysis bullosa. In some embodiments, the treatment comprises administering to a subject a nucleic acid encoding a gene-editing protein that is capable of correcting a mutation in or near the COL7A1 gene. In one embodiment, the correction increases the amount of functional collagen VII produced by the subject's cells. In another embodiment, the treatment reduces the size, severity, and/or frequency of recurrence of skin lesions and/or blisters. Still other embodiments are directed to a treatment for primary hyperoxaluria. In one embodiment the primary hyperoxaluria is type I primary hyperoxaluria. In another embodiment, the treatment comprises administering to a subject a nucleic acid encoding a gene-editing protein that is capable of correcting a mutation in or near the AGXT gene. In yet another embodiment, the treatment delays or eliminates the subject's need for a kidney and/or liver transplant.

Genes that can be edited according to the methods of the present invention to produce therapeutics of the present invention include genes that can be edited to restore normal function, as well as genes that can be edited to reduce or eliminate function. Such genes include, but are not limited to alpha-1-antitrypsin (SERPINA1), mutations in which can cause alpha-1-antitrypsin deficiency, beta globin (HBB), mutations in which can cause sickle cell disease (SCD) and β-thalassemia, breast cancer 1, early onset (BRCA1) and breast cancer 2, early onset (BRCA2), mutations in which can increase susceptibility to breast cancer, C-C chemokine receptor type 5 (CCR5) and C-X-C chemokine receptor type 4 (CXCR4), mutations in which can confer resistance to HIV infection, cystic fibrosis transmembrane conductance regulator (CFTR), mutations in which can cause cystic fibrosis, dystrophin (DMD), mutations in which can cause muscular dystrophy, including Duchenne muscular dystrophy and Becker's muscular dystrophy, glutamate decarboxylase 1 and glutamate decarboxylase 2 (GAD1, GAD2), mutations in which can prevent autoimmune destruction of β-cells, hemoglobin alpha 1, hemoglobin alpha 2, and hemoglobin delta (HBA1, HBA2, and HBD), mutations in which can cause thalassemia, desmoplakin, keratin 5, keratin 14, plectin, integrin alpha-6, integrin beta-4, laminin subunit alpha-3, laminin subunit beta-3, laminin subunit gamma-2, collagen type VII alpha 1, collagen type XVII alpha 1, and matrix metalloproteinase-1 (DSP, KRT5, KRT14, PLEC1, ITGA6, ITGB4, LAMA3, LAMB3, LAMC2, COL7A1, COL17A1, and MM P1), mutations in which can cause epidermolysis bullosa, Huntington (HTT), mutations in which can cause Huntington's disease, superoxide dismutase 1 (SOD1), mutations in which can cause amyotrophic lateral sclerosis (ALS), XPA, XPB, XPC, XPD (ERCC6) and polymerase (DNA directed), eta (POLH), mutations in which can cause xeroderma pigmentosum, leucine-rich repeat kinase 2 (LRRK2), mutations in which can cause Parkinson's disease, and Fanconi anemia, complementation groups A, B, C, D1, D2, E, F, G, I, J, L, M, N, P (FANCA, FANCB, FANCC, FANCD1, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ, FANCL, FANCM, FANCN, FANCP), and RAD51 homolog C (S. cerevisiae) (RAD51C), mutations in which can cause Fanconi anemia.

In a specific embodiment, the present invention relates to method of modulating Growth-Factor-Differentiation-Factor-15 (GDF15). In a specific embodiment, there is provided a method of treatment in which an RNA as described herein is administered to modulate GDF15 levels. In some embodiments, the RNA encodes GDF15. In a specific embodiment, the gene that is edited is Growth-Factor-Differentiation-Factor-15 (GDF15). In various embodiments, the present invention relates to targeting and/or expressing GDF15 to treat or prevent a disease or disorder such as a metabolic disease or disorder (e.g., diabetes (type I and II), insulin resistance, obesity, dyslipidemia, hypercholesterolemia, hyperglycemia, hyperinsulinemia, hypertension, hepatosteaotosis such as non-alcoholic steatohepatitis (NASH) and non-alcoholic fatty acid liver disease (NAFLD), cancer, a disease or disorder associated with impaired lipid metabolism, a disease or disorder associated with impaired renal function (e.g., chronic kidney diseases, nephropathy such as diabetic nephropathy, kidney failure), a disease or disorder associated with impaired hepatic function, a disease or disorder associated with impaired lung function, a vascular or cardiovascular disease or disorder (e.g., coronary artery disease, cardiomyopathy, hypertension, atrial fibrillation, preeclampsia, peripheral artery disease, atherosclerosis, heart failure, acute myocardial infarction, acute coronary syndrome, muscle wasting, hypertensive ventricular hypertrophy, hypertensive cardiomyopathy, ischemic heart disease, myocardial infarction, abdominal aortic aneurysm, a blood clot, deep vein thrombosis, venous stasis disease, phlebitis, varicose veins etc.), muscle wasting, inflammation, and a respiratory disease. In a specific embodiment, targeting of GDF15 improves lipid metabolism by mobilizing lipid deposits from the liver and/or other tissues into circulation for use in metabolism.

In certain disorders, the body may increase GDF15 levels in an attempt to counteract one or more negative aspects of the disease state. Certain embodiments are therefore directed to increasing GDF15 levels. In one embodiment, the serum level of GDF15 is increased. In another embodiment, the level of GDF15 in a tissue and/or organ is increased. In yet another embodiment, ALT levels are reduced. In yet another embodiment, AST levels are reduced. In still another embodiment, glucose levels are reduced. In still another embodiment, serum cholesterol levels are increased. In still another embodiment, serum triglyceride levels are increased. In yet another embodiment, food intake is reduced. In yet another embodiment, bodyweight is reduced. In a further embodiment, treatment improves adherence to a diet and/or exercise regimen.

In a specific embodiment, expressing GDF15 improves lipid metabolism by mobilizing lipid deposits from the liver and/or other tissues into circulation for use in metabolism. In a specific embodiment, expressing and/or targeting of GDF15 reduces liver inflammation. In a specific embodiment, expressing and/or targeting of GDF15 treats or prevents one or more of fatty liver, NAFLD, NASH, inflammation, hepatitis, fibrosis, cirrhosis, and hepatocellular carcinoma. In a specific embodiment, expressing and/or targeting of GDF15 treats or prevents one or more of chronic kidney disease, acute kidney injury, nephropathy, diabetic nephropathy, kidney failure, and kidney fibrosis. In a specific embodiment, expressing and/or targeting GDF15 in combination with expressing and/or targeting another member of the TGF-β superfamily improves lipid metabolism by mobilizing lipid deposits from the liver and/or other tissues into circulation for use in metabolism, reduces liver inflammation, and/or treats or prevents one or more of fatty liver, NAFLD, NASH, inflammation, hepatitis, fibrosis, cirrhosis, hepatocellular carcinoma, chronic kidney disease, acute kidney injury, nephropathy, diabetic nephropathy, kidney failure, and kidney fibrosis. Certain embodiments are directed to a therapeutic comprising a nucleic acid. In one embodiment, the nucleic acid encodes one or more gene-editing proteins. Other embodiments are directed to a therapeutic comprising one or more cells that are transfected, reprogrammed, and/or gene-edited in vivo according to the methods of the present invention. In one embodiment, a cell is transfected, reprogrammed, and/or gene-edited, and the transfected, reprogrammed, and/or gene-edited cell is introduced into a patient. In another embodiment, the cell is harvested from the same patient into whom the transfected, reprogrammed and/or gene-edited cell is introduced. Examples of diseases that can be treated with therapeutics of the present invention include, but are not limited to Alzheimer's disease, spinal cord injury, amyotrophic lateral sclerosis, cystic fibrosis, heart disease, including ischemic and dilated cardiomyopathy, macular degeneration, Parkinson's disease, Huntington's disease, diabetes, sickle-cell anemia, thalassemia, Fanconi anemia, xeroderma pigmentosum, muscular dystrophy, severe combined immunodeficiency, hereditary sensory neuropathy, cancer, and HIV/AIDS. In certain embodiments, the therapeutic comprises a cosmetic. In one embodiment, a cell is harvested from a patient, the cell is reprogrammed and expanded to a large number of adipose cells to produce a cosmetic, and the cosmetic is introduced into the patient. In still another embodiment, the cosmetic is used for tissue reconstruction.

While detailed examples are provided herein for the production of specific types of cells and for the production of therapeutics comprising specific types of cells, it is recognized that the methods of the present invention can be used to produce many other types of cells, and to produce therapeutics comprising one or more of many other types of cells, for example, by reprogramming a cell according to the methods of the present invention, and culturing the cell under conditions that mimic one or more aspects of development by providing conditions that resemble the conditions present in the cellular microenvironment during development.

Certain embodiments are directed to a library of cells with a variety of human leukocyte antigen (HLA) types (“HLA-matched libraries”). An HLA-matched library may be beneficial in part because it can provide for the rapid production and/or distribution of therapeutics without the patient having to wait for a therapeutic to be produced from the patient's cells. Such a library may be particularly beneficial for the production of cosmetics and for the treatment of heart disease and diseases of the blood and/or immune system for which patients may benefit from the immediate availability of a therapeutic or cosmetic.

The DNA sequence of a cell can be altered by contacting the cell with a gene-editing protein or by inducing the cell to express a gene-editing protein. However, previously disclosed gene-editing proteins suffer from low binding efficiency and excessive off-target activity, which can introduce undesired mutations in the DNA of the cell, severely limiting their use in vivo, for example in therapeutic and cosmetic applications, in which the introduction of undesired mutations in a patient's cells could lead to the development of cancer. It has now been discovered that gene-editing proteins that comprise the StsI endonuclease cleavage domain (SEQ ID NO: 1) can exhibit substantially lower off-target activity in vivo than previously disclosed gene-editing proteins, while maintaining a high level of on-target activity in vivo. Other novel engineered proteins have also been discovered that can exhibit high on-target activity in vivo, low off-target activity in vivo, small size, solubility, and other desirable characteristics when they are used as the nuclease domain of a gene-editing protein: StsI-HA (SEQ ID NO: 2), StsI-HA2 (SEQ ID NO: 3), StsI-UHA (SEQ ID NO: 4), StsI-UHA2 (SEQ ID NO: 5), StsI-HF (SEQ ID NO: 6), and StsI-UHF (SEQ ID NO: 7). StsI-HA, StsI-HA2 (high activity), StsI-UHA, and StsI-UHA2 (ultra-high activity) can exhibit higher on-target activity in vivo than both wild-type StsI and wild-type FokI, due in part to specific amino-acid substitutions within the N-terminal region at the 34 and 61 positions, while StsI-HF (high fidelity) and StsI-UHF (ultra-high fidelity) can exhibit lower off-target activity in vivo than both wild-type StsI and wild-type FokI, due in part to specific amino-acid substitutions within the C-terminal region at the 141 and 152 positions.

Certain embodiments are therefore directed to a protein. In some embodiments, the protein is present in vivo. In other embodiments, the protein comprises a nuclease domain. In one embodiment, the nuclease domain comprises one or more of: the cleavage domain of FokI endonuclease (SEQ ID NO: 53), the cleavage domain of StsI endonuclease (SEQ ID NO: 1), StsI-HA (SEQ ID NO: 2), StsI-HA2 (SEQ ID NO: 3), StsI-UHA (SEQ ID NO: 4), StsI-UHA2 (SEQ ID NO: 5), StsI-HF (SEQ ID NO: 6), and StsI-UHF (SEQ ID NO: 7) or a biologically active fragment or variant thereof.

It has also been discovered that engineered gene-editing proteins that comprise DNA-binding domains comprising certain novel repeat sequences can exhibit lower off-target activity in vivo than previously disclosed gene-editing proteins, while maintaining a high level of on-target activity in vivo. Certain of these engineered gene-editing proteins can provide several advantages over previously disclosed gene-editing proteins, including, for example, increased flexibility of the linker region connecting repeat sequences, which can result in increased binding efficiency. Certain embodiments are therefore directed to a protein comprising a plurality of repeat sequences. In one embodiment, at least one of the repeat sequences contains the amino-acid sequence: GabG, where “a” and “b” each represent any amino acid. In one embodiment, the protein is a gene-editing protein. In another embodiment, one or more of the repeat sequences are present in a DNA-binding domain. In a further embodiment, “a” and “b” are each independently selected from the group: H and G. In a still further embodiment, “a” and “b” are H and G, respectively. In one embodiment, the amino-acid sequence is present within about 5 amino acids of the C-terminus of the repeat sequence. In another embodiment, the amino-acid sequence is present at the C-terminus of the repeat sequence. In some embodiments, one or more G in the amino-acid sequence GabG is replaced with one or more amino acids other than G, for example A, H or GG. In one embodiment, the repeat sequence has a length of between about 32 and about 40 amino acids or between about 33 and about 39 amino acids or between about 34 and 38 amino acids or between about 35 and about 37 amino acids or about 36 amino acids or greater than about 32 amino acids or greater than about 33 amino acids or greater than about 34 amino acids or greater than about 35 amino acids. Other embodiments are directed to a protein comprising one or more transcription activator-like effector domains. In one embodiment, at least one of the transcription activator-like effector domains comprises a repeat sequence. Other embodiments are directed to a protein comprising a plurality of repeat sequences generated by inserting one or more amino acids between at least two of the repeat sequences of a transcription activator-like effector domain. In one embodiment, one or more amino acids is inserted about 1 or about 2 or about 3 or about 4 or about 5 amino acids from the C-terminus of at least one repeat sequence. Still other embodiments are directed to a protein comprising a plurality of repeat sequences, wherein about every other repeat sequence has a different length than the repeat sequence immediately preceding or following the repeat sequence. In one embodiment, every other repeat sequence is about 36 amino acids long. In another embodiment, every other repeat sequence is 36 amino acids long. Still other embodiments are directed to a protein comprising a plurality of repeat sequences, wherein the plurality of repeat sequences comprises at least two repeat sequences that are each at least 36 amino acids long, and wherein at least two of the repeat sequences that are at least 36 amino acids long are separated by at least one repeat sequence that is less than 36 amino acids long. Some embodiments are directed to a protein that comprises one or more sequences selected from, for example, SEQ ID NO: 54, SEQ ID NO: 55, SEQ ID NO: 56, SEQ ID NO: 57, SEQ ID NO: 58, SEQ ID NO: 59, and SEQ ID NO: 60.

Other embodiments are directed to a protein that comprises a DNA-binding domain. In some embodiments, the DNA-binding domain comprises a plurality of repeat sequences. In one embodiment, the plurality of repeat sequences enables high-specificity recognition of a binding site in a target DNA molecule. In another embodiment, at least two of the repeat sequences have at least about 50%, or about 60%, or about 70%, or about 80%, or about 90%, or about 95%, or about 98%, or about 99% homology to each other. In a further embodiment, at least one of the repeat sequences comprises one or more regions capable of binding to a binding site in a target DNA molecule. In a still further embodiment, the binding site comprises a defined sequence of between about 1 to about 5 bases in length. In one embodiment, the DNA-binding domain comprises a zinc finger. In another embodiment, the DNA-binding domain comprises a transcription activator-like effector (TALE). In a further embodiment, the plurality of repeat sequences includes at least one repeat sequence having at least about 50% or about 60% or about 70% or about 80% or about 90% or about 95% or about 98%, or about 99% homology to a TALE. In a still further embodiment, the gene-editing protein comprises a clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein. In one embodiment, the gene-editing protein comprises a nuclear-localization sequence. In another embodiment, the nuclear-localization sequence comprises the amino-acid sequence: PKKKRKV (SEQ ID NO: 471). In one embodiment, the gene-editing protein comprises a mitochondrial-localization sequence. In another embodiment, the mitochondrial-localization sequence comprises the amino-acid sequence: LGRVIPRKIASRASLM (SEQ ID NO: 472). In one embodiment, the gene-editing protein comprises a linker. In another embodiment, the linker connects a DNA-binding domain to a nuclease domain. In a further embodiment, the linker is between about 1 and about 10 amino acids long. In some embodiments, the linker is about 1, about 2, or about 3, or about 4, or about 5, or about 6, or about 7, or about 8, or about 9, or about 10 amino acids long. In one embodiment, the gene-editing protein is capable of generating a nick or a double-strand break in a target DNA molecule.

Certain embodiments are directed to a method for modifying the genome of a cell in vivo, the method comprising introducing into a cell in vivo a nucleic acid molecule encoding a non-naturally occurring fusion protein comprising an artificial transcription activator-like (TAL) effector repeat domain comprising one or more repeat units 36 amino acids in length and an endonuclease domain, wherein the repeat domain is engineered for recognition of a predetermined nucleotide sequence, and wherein the fusion protein recognizes the predetermined nucleotide sequence. In one embodiment, the cell is a eukaryotic cell. In another embodiment, the cell is an animal cell. In a further embodiment, the cell is a mammalian cell. In a still further embodiment, the cell is a human cell. In one embodiment, the cell is a plant cell. In another embodiment, the cell is a prokaryotic cell. In some embodiments, the fusion protein introduces an endonucleolytic cleavage in a nucleic acid of the cell, whereby the genome of the cell is modified.

Certain embodiments are directed to a composition for altering the DNA sequence of a cell in vivo comprising a nucleic acid, wherein the nucleic acid encodes a gene-editing protein. Other embodiments are directed to a composition for altering the DNA sequence of a cell in vivo comprising a nucleic-acid mixture, wherein the nucleic-acid mixture comprises: a first nucleic acid that encodes a first gene-editing protein, and a second nucleic acid that encodes a second gene-editing protein. In one embodiment, the binding site of the first gene-editing protein and the binding site of the second gene-editing protein are present in the same target DNA molecule. In another embodiment, the binding site of the first gene-editing protein and the binding site of the second gene-editing protein are separated by less than about 50 bases, or less than about 40 bases, or less than about 30 bases or less than about 20 bases, or less than about 10 bases, or between about 10 bases and about 25 bases or about 15 bases. In one embodiment, the nuclease domain of the first gene-editing protein and the nuclease domain of the second gene-editing protein are capable of forming a dimer. In another embodiment, the dimer is capable of generating a nick or double-strand break in a target DNA molecule.

Certain embodiments are directed to a therapeutic composition. Other embodiments are directed to a cosmetic composition. In some embodiments, the composition comprises a repair template. In a further embodiment, the repair template is a single-stranded DNA molecule or a double-stranded DNA molecule.

Other embodiments are directed to an article of manufacture for synthesizing a protein or a nucleic acid encoding a protein. In one embodiment, the article is a nucleic acid. In another embodiment, the protein comprises a DNA-binding domain. In a further embodiment, the nucleic acid comprises a nucleotide sequence encoding a DNA-binding domain. In one embodiment, the protein comprises a nuclease domain. In another embodiment, the nucleic acid comprises a nucleotide sequence encoding a nuclease domain. In one embodiment, the protein comprises a plurality of repeat sequences. In another embodiment, the nucleic acid encodes a plurality of repeat sequences. In a further embodiment, the nuclease domain is selected from: FokI, StsI, StsI-HA, StsI-HA2, StsI-UHA, StsI-UHA2, StsI-HF, and StsI-UHF or a natural or engineered variant or biologically active fragment thereof. In one embodiment, the nucleic acid comprises an RNA-polymerase promoter. In another embodiment, the RNA-polymerase promoter is a T7 promoter or a SP6 promoter. In a further embodiment, the nucleic acid comprises a viral promoter. In one embodiment, the nucleic acid comprises an untranslated region. In another embodiment, the nucleic acid is an in vitro-transcription template.

Certain embodiments are directed to a method for inducing a cell to express a protein in vivo. Other embodiments are directed to a method for altering the DNA sequence of a cell in vivo comprising transfecting the cell in vivo with a gene-editing protein or inducing the cell to express a gene-editing protein in vivo. Still other embodiments are directed to a method for reducing the expression of a protein of interest in a cell in vivo. In one embodiment, the cell is induced to express a gene-editing protein, wherein the gene-editing protein is capable of creating a nick or a double-strand break in a target DNA molecule. In another embodiment, the nick or double-strand break results in inactivation of a gene. Still other embodiments are directed to a method for generating an inactive, reduced-activity or dominant-negative form of a protein in vivo. In one embodiment, the protein is survivin. Still other embodiments are directed to a method for repairing one or more mutations in a cell in vivo. In one embodiment, the cell is contacted with a repair template. In another embodiment, the repair template is a DNA molecule. In a further embodiment, the repair template does not contain a binding site of the gene-editing protein. In a still further embodiment, the repair template encodes an amino-acid sequence that is encoded by a DNA sequence that comprises a binding site of the gene-editing protein.

In various embodiments, the repair template is about 20 nucleotides, or about 30 nucleotides, or about 40 nucleotides, or about 50 nucleotides, or about 60 nucleotides, or about 70 nucleotides, or about 80 nucleotides, or about 90 nucleotides, or about 100 nucleotides, or about 150 nucleotides, or about 200 nucleotides, or about 300 nucleotides, or about 400 nucleotides, or about 500 nucleotides, or about 750 nucleotides, or about 1000 nucleotides. In various embodiments, the repair template is about 20-1000 nucleotides, or about 20-500 nucleotides, or about 20-400 nucleotides, or about 20-200 nucleotides, or about 20-100 nucleotides, or about 80-100 nucleotides, or about 50-100 nucleotides.

In various embodiments, the mass ratio of RNA (e.g. synthetic RNA encoding gene-editing protein) to repair template is about 1:10, or about 1:9, or about 1:8, or about 1:7, or about 1:6, or about 1:5, or about 1:4, or about 1:3, or about 1:2, or about 1:1, or about 2:1, or about 3:1, or about 4:1, or about 5:1, or about 6:1, or about 7:1, or about 8:1, or about 9:1, or about 10:1.

In various embodiments, the molar ratio of RNA (e.g. synthetic RNA encoding gene-editing protein) to repair template is about 1:10, or about 1:9, or about 1:8, or about 1:7, or about 1:6, or about 1:5, or about 1:4, or about 1:3, or about 1:2, or about 1:1, or about 2:1, or about 3:1, or about 4:1, or about 5:1, or about 6:1, or about 7:1, or about 8:1, or about 9:1, or about 10:1.

In various embodiments, the repair template has a dual function, causing a repair to a gene-edited target sequence and preventing further binding of a gene-editing protein, thereby reducing or eliminating further gene-editing (e.g. via the repair template causing a repair that renders what was the gene-editing protein binding site no longer suitable for gene-editing protein binding). Accordingly, in some embodiments, the present gene-editing methods are tunable to ensure a single gene-edit per target site.

Other embodiments are directed to a method for treating a patient comprising administering to the patient a therapeutically or cosmetically effective amount of a protein or a nucleic acid encoding a protein. In one embodiment, the treatment results in one or more of the patient's symptoms being ameliorated. Certain embodiments are directed to a method for treating a patient comprising: a. inducing a cell to express a protein of interest by transfecting the cell in vivo with a nucleic acid encoding the protein of interest and/or b. reprogramming the cell in vivo. In one embodiment, the cell is reprogrammed to a less differentiated state. In another embodiment, the cell is reprogrammed by transfecting the cell with one or more synthetic RNA molecules encoding one or more reprogramming proteins. In a further embodiment, the cell is differentiated. In a still further embodiment, the cell is differentiated into one of: a skin cell, a glucose-responsive insulin-producing cell, a hematopoietic cell, a cardiac cell, a retinal cell, a renal cell, a neural cell, a stromal cell, a fat cell, a bone cell, a muscle cell, an oocyte, and a sperm cell. Other embodiments are directed to a method for treating a patient comprising: a. inducing a cell to express a gene-editing protein by transfecting the cell in vivo with a nucleic acid encoding a gene-editing protein and/or b. reprogramming the cell in vivo.

Other embodiments are directed to a complexation medium. In one embodiment, the complexation medium has a pH greater than about 7, or greater than about 7.2, or greater than about 7.4, or greater than about 7.6, or greater than about 7.8, or greater than about 8.0, or greater than about 8.2, or greater than about 8.4, or greater than about 8.6, or greater than about 8.8, or greater than about 9.0. In another embodiment, the complexation medium comprises transferrin. In a further embodiment, the complexation medium comprises DMEM. In a still further embodiment, the complexation medium comprises DMEM/F12. Still other embodiments are directed to a method for forming nucleic-acid-transfection-reagent complexes. In one embodiment, the transfection reagent is incubated with a complexation medium. In another embodiment, the incubation occurs before a mixing step. In a further embodiment, the incubation step is between about 5 seconds and about 5 minutes or between about 10 seconds and about 2 minutes or between about 15 seconds and about 1 minute or between about 30 seconds and about 45 seconds. In one embodiment, the transfection reagent is selected from Table 1. In another embodiment, the transfection reagent is a lipid or lipidoid. In a further embodiment, the transfection reagent comprises a cation. In a still further embodiment, the cation is a multivalent cation. In a still further embodiment, the transfection reagent is N1-[2-((1S)-1-[(3-aminopropyl)amino]-4-[di(3-amino-propyl)amino]butylcarboxamido)ethyl]-3,4-di[oleyloxy]-benzamide (a.k.a. MVL5) or a derivative thereof.

Certain embodiments are directed to a method for inducing a cell to express a protein by contacting the cell with a nucleic acid in vivo. In one embodiment, the cell is a mammalian cell. In another embodiment, the cell is a human cell or a rodent cell. Other embodiments are directed to a cell produced using one or more of the methods of the present invention. In one embodiment, the cell is present in a patient. In another embodiment, the cell is isolated from a patient. Other embodiments are directed to a screening library comprising a cell produced using one or more of the methods of the present invention. In one embodiment, the screening library is used for at least one of: toxicity screening, including: cardiotoxicity screening, neurotoxicity screening, and hepatotoxicity screening, efficacy screening, high-throughput screening, high-content screening, and other screening.

Other embodiments are directed to a kit containing a nucleic acid. In one embodiment, the kit contains a delivery reagent (a.k.a. “transfection reagent”). In another embodiment, the kit is a reprogramming kit. In a further embodiment, the kit is a gene-editing kit. Other embodiments are directed to a kit for producing nucleic acids. In one embodiment, the kit contains at least two of: pseudouridine-triphosphate, 5-methyluridine triphosphate, 5-methylcytidine triphosphate, 5-hydroxymethylcytidine triphosphate, N4-methylcytidine triphosphate, N4-acetylcytidine triphosphate, and 7-deazaguanosine triphosphate or one or more derivatives thereof. Other embodiments are directed to a therapeutic or cosmetic comprising a nucleic acid. In one embodiment, the therapeutic or cosmetic is a pharmaceutical composition. In another embodiment, the pharmaceutical composition is formulated. In a further embodiment, the formulation comprises an aqueous suspension of liposomes. Example liposome components are set forth in Table 1, and are given by way of example, and not by way of limitation. In one embodiment, the liposomes include one or more polyethylene glycol (PEG) chains. In another embodiment, the PEG is PEG2000. In a further embodiment, the liposomes include 1,2-distearoyl-sn-glycero-3-phosphoethanolamine (DSPE) or a derivative thereof. In one embodiment, the therapeutic comprises one or more ligands. In another embodiment, the therapeutic comprises at least one of: androgen, CD30 (TNFRSF8), a cell-penetrating peptide, CXCR, estrogen, epidermal growth factor, EGFR, HER2, folate, insulin, insulin-like growth factor-I, interleukin-13, integrin, progesterone, stromal-derived-factor-1, thrombin, vitamin D, and transferrin or a biologically active fragment or variant thereof. Still other embodiments are directed to a therapeutic or cosmetic comprising a cell generated using one or more of the methods of the present invention. In one embodiment, the therapeutic is administered to a patient for the treatment of any of the diseases or disorders described herein, including by way of non-limitation, type 1 diabetes, heart disease, including ischemic and dilated cardiomyopathy, macular degeneration, Parkinson's disease, cystic fibrosis, sickle-cell anemia, thalassemia, Fanconi anemia, severe combined immunodeficiency, hereditary sensory neuropathy, xeroderma pigmentosum, Huntington's disease, muscular dystrophy, amyotrophic lateral sclerosis, Alzheimer's disease, cancer, and infectious diseases including: hepatitis and HIV/AIDS.

Other embodiments are directed to a method for reprogramming a cell in vivo. In one embodiment, the cell is reprogrammed by contacting the cell with one or more nucleic acids. In one embodiment, the cell is contacted with a plurality of nucleic acids encoding at least one of: Oct4 protein, Sox2 protein, Klf4 protein, c-Myc protein, Lin28 protein or a biologically active fragment, variant or derivative thereof. In another embodiment, the cell is contacted with a plurality of nucleic acids encoding a plurality of proteins including: Oct4 protein, Sox2 protein, Klf4 protein, and c-Myc protein or one or more biologically active fragments, variants or derivatives thereof. Still other embodiments are directed to a method for gene editing a cell in vivo. In one embodiment, the cell is gene-edited by contacting the cell with one or more nucleic acids.

Certain embodiments are directed to a method for inducing a cell in vivo to express a protein of interest comprising contacting a cell in vivo with a solution comprising albumin that is treated with an ion-exchange resin or charcoal and one or more nucleic acid molecules, wherein at least one of the one or more nucleic acid molecules encodes a protein of interest. In one embodiment, the method results in the cell expressing the protein of interest. In another embodiment, the one or more nucleic acid molecules comprise a synthetic RNA molecule. In one embodiment, the cell is a skin cell. In another embodiment, the cell is a muscle cell. In yet another embodiment, the cell is a dermal fibroblast. In yet another embodiment, the cell is a myoblast. In one embodiment, the protein of interest is an extracellular matrix protein. In another embodiment, the protein of interest is selected from: elastin, collagen, laminin, fibronectin, vitronectin, lysyl oxidase, elastin binding protein, a growth factor, fibroblast growth factor, transforming growth factor beta, granulocyte colony-stimulating factor, a matrix metalloproteinase, an actin, fibrillin, microfibril-associated glycoprotein, a lysyl-oxidase-like protein, and platelet-derived growth factor. In one embodiment, the solution is delivered to the dermis. In another embodiment, the delivering is by injection. In yet another embodiment, the delivering is by injection using a microneedle array. In one embodiment, the solution further comprises a growth factor. In another embodiment, the growth factor is selected from: fibroblast growth factor and transforming growth factor beta. In yet another embodiment, the solution further comprises cholesterol. Other embodiments are directed a method for inducing a cell in vivo to express a protein of interest comprising contacting a cell in vivo with a solution comprising cholesterol and one or more nucleic acid molecules, wherein at least one of the one or more nucleic acid molecules encodes a protein of interest. In one embodiment, the method results in the cell expressing the protein of interest. Still other embodiments are directed to a method for transfecting a cell in vivo with a nucleic acid molecule comprising contacting a cell in vivo with a solution comprising albumin that is treated with an ion-exchange resin or charcoal and a nucleic acid molecule. In one embodiment, the method results in the cell being transfected with the nucleic acid molecule. In another embodiment, the nucleic acid molecule is one of: a dsDNA molecule, a ssDNA molecule, a dsRNA molecule, a ssRNA molecule, a plasmid, an oligonucleotide, a synthetic RNA molecule, a miRNA molecule, an mRNA molecule, an siRNA molecule. Still other embodiments are directed to a method for treating a patient comprising delivering to a patient a composition comprising albumin that is treated with an ion-exchange resin or charcoal and one or more nucleic acid molecules, wherein at least one of the one or more nucleic acid molecules encodes a protein of interest. In one embodiment, the method results in the expression of the protein of interest in the patient. In another embodiment, the method results in the expression of the protein of interest in the dermis of the patient.

Certain embodiments are directed to a cosmetic composition comprising albumin that is treated with an ion-exchange resin or charcoal and a nucleic acid molecule. Other embodiments are directed to a cosmetic treatment article. In one embodiment, the cosmetic treatment article comprises a device configured to deliver a composition to a patient. In another embodiment, the nucleic acid molecule encodes elastin protein or collagen protein. Still other embodiments are directed to a solution for transfecting a cell in vivo comprising cholesterol or a cholesterol analog and one or more nucleic acid molecules. In one embodiment, the cholesterol or cholesterol analog is covalently bound to at least one of the one or more nucleic acid molecules. In another embodiment, the cholesterol analog is an oxysterol. In yet another embodiment, the cholesterol analog includes one or more of: an A-ring substitution, a B-ring substitution, a D-ring substitution, a side-chain substitution, a cholestanoic acid, a cholestenoic acid, a polyunsaturated moiety, a deuterated moiety, a fluorinated moiety, a sulfonated moiety, a phosphorylated moiety, and a fluorescent moiety. In yet another embodiment, the method comprises treating the patient with one or more of: a dermal filler, a neurotoxin (by way of illustration sodium channel inhibitors (e.g., tetrodotoxin), potassium channel inhibitors (e.g., tetraethylammonium), chloride channel inhibitors (e.g., chlorotoxin and curare), calcium channel inhibitors (e.g., conotoxin), synaptic vesicle release inhibitors (e.g., botulinum toxin and tetanus toxin) and blood brain barrier inhibitor (e.g., aluminum and mercury)) and a repair-inducing treatment.

Despite the tendency of transfection reagent nucleic acid complexes to precipitate, form clumps or otherwise degrade when stored for more than a few minutes, the present inventors have surprisingly discovered that transfection reagent nucleic acid complexes produced according to some embodiments of the present invention can be frozen and/or can be stored at various temperatures, including room temperature, about 4° C., about −20° C., about −80° C., and about −196° C. for an extended period of time, for example, for several hours, about 1 day, about 1 week, about 1 month, about 1 year, and longer than about 1 year. Some embodiments are therefore directed to a pharmaceutical formulation comprising synthetic RNA and a transfection reagent, wherein the pharmaceutical formulation is provided in solid form. Other embodiments are directed to a pharmaceutical formulation comprising synthetic RNA transfection reagent complexes, wherein the synthetic RNA transfection reagent complexes are provided in solid form. In various embodiments, the synthetic RNA transfection reagent complexes are provided in frozen form. Various embodiments are directed to a method for stabilizing nucleic acid transfection reagent complexes comprising forming nucleic acid transfection reagent complexes and contacting the nucleic acid transfection reagent complexes or vessel in which such are contained with a cryogenic liquid to produce stabilized nucleic acid transfection reagent complexes. In one embodiment, the nucleic acid transfection reagent complexes are stabilized for shipment or storage.

Illustrative subjects or patients refers to any vertebrate including, without limitation, humans and other primates (e.g., chimpanzees and other apes and monkey species), farm animals (e.g., cattle, sheep, pigs, goats, and horses), domestic mammals (e.g., dogs and cats), laboratory animals (e.g., rodents such as mice, rats, and guinea pigs), and birds (e.g., domestic, wild and game birds such as chickens, turkeys and other gallinaceous birds, ducks, geese, and the like). In some embodiments, the subject is a mammal. In some embodiments, the subject is a human.

Definitions

By “molecule” is meant a molecular entity (molecule, ion, complex, etc.).

By “RNA molecule” is meant a molecule that comprises RNA.

By “synthetic RNA molecule” is meant an RNA molecule that is produced outside of a cell or that is produced inside of a cell using bioengineering, by way of non-limiting example, an RNA molecule that is produced in an in vitro-transcription reaction, an RNA molecule that is produced by direct chemical synthesis or an RNA molecule that is produced in a genetically-engineered E. coli cell.

By “transfection” is meant contacting a cell with a molecule, wherein the molecule is internalized by the cell.

By “upon transfection” is meant during or after transfection.

By “transfection reagent” is meant a substance or mixture of substances that associates with a molecule and facilitates the delivery of the molecule to and/or internalization of the molecule by a cell, by way of non-limiting example, a cationic lipid, a charged polymer or a cell-penetrating peptide.

By “reagent-based transfection” is meant transfection using a transfection reagent.

By “medium” is meant a solvent or a solution comprising a solvent and a solute, by way of non-limiting example, Dulbecco's Modified Eagle's Medium (DMEM), DMEM+10% fetal bovine serum (FBS), saline or water.

By “complexation medium” is meant a medium to which a transfection reagent and a molecule to be transfected are added and in which the transfection reagent associates with the molecule to be transfected.

By “transfection medium” is meant a medium that can be used for transfection, by way of non-limiting example, Dulbecco's Modified Eagle's Medium (DMEM), DMEM/F12, saline or water.

By “recombinant protein” is meant a protein or peptide that is not produced in animals or humans. Non-limiting examples include human transferrin that is produced in bacteria, human fibronectin that is produced in an in vitro culture of mouse cells, and human serum albumin that is produced in a rice plant.

By “Oct4 protein” is meant a protein that is encoded by the POU5F1 gene, or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof, by way of non-limiting example, human Oct4 protein (SEQ ID NO: 8), mouse Oct4 protein, Oct1 protein, a protein encoded by POU5F1 pseudogene 2, a DNA-binding domain of Oct4 protein or an Oct4-GFP fusion protein. In some embodiments the Oct4 protein comprises an amino acid sequence that has at least 70% identity with SEQ ID NO: 8, or in other embodiments, at least 75%, 80%, 85%, 90%, or 95% identity with SEQ ID NO: 8. In some embodiments, the Oct4 protein comprises an amino acid sequence having from 1 to 20 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 8. Or in other embodiments, the Oct4 protein comprises an amino acid sequence having from 1 to 15 or from 1 to 10 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 8.

By “Sox2 protein” is meant a protein that is encoded by the SOX2 gene, or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof, by way of non-limiting example, human Sox2 protein (SEQ ID NO: 9), mouse Sox2 protein, a DNA-binding domain of Sox2 protein or a Sox2-GFP fusion protein. In some embodiments the Sox2 protein comprises an amino acid sequence that has at least 70% identity with SEQ ID NO: 9, or in other embodiments, at least 75%, 80%, 85%, 90% or 95% identity with SEQ ID NO: 9. In some embodiments, the Sox2 protein comprises an amino acid sequence having from 1 to 20 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 9. Or in other embodiments, the Sox2 protein comprises an amino acid sequence having from 1 to 15 or from 1 to 10 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 9.

By “Klf4 protein” is meant a protein that is encoded by the KLF4 gene, or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof, by way of non-limiting example, human Klf4 protein (SEQ ID NO: 10), mouse Klf4 protein, a DNA-binding domain of Klf4 protein or a Klf4-GFP fusion protein. In some embodiments the Klf4 protein comprises an amino acid sequence that has at least 70% identity with SEQ ID NO: 10, or in other embodiments, at least 75%, 80%, 85%, 90%, or 95% identity with SEQ ID NO: 10. In some embodiments, the Klf4 protein comprises an amino acid sequence having from 1 to 20 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 10. Or in other embodiments, the Klf4 protein comprises an amino acid sequence having from 1 to 15 or from 1 to 10 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 10.

By “c-Myc protein” is meant a protein that is encoded by the MYC gene, or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof, by way of non-limiting example, human c-Myc protein (SEQ ID NO: 11), mouse c-Myc protein, I-Myc protein, c-Myc (T58A) protein, a DNA-binding domain of c-Myc protein or a c-Myc-GFP fusion protein. In some embodiments the c-Myc protein comprises an amino acid sequence that has at least 70% identity with SEQ ID NO: 11, or in other embodiments, at least 75%, 80%, 85%, 90%, or 95% identity with SEQ ID NO: 11. In some embodiments, the c-Myc protein comprises an amino acid having from 1 to 20 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 11. Or in other embodiments, the c-Myc protein comprises an amino acid sequence having from 1 to 15 or from 1 to 10 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 11.

By “erythropoietin” or “erythropoietin protein” is meant a protein that is encoded by the EPO gene, or a natural or engineered variant, family-member, orthologue, fragment or fusion construct thereof, by way of non-limiting example, human erythropoietin (SEQ ID NO: 164), mouse erythropoietin, darbepoetin, darbepoetin alfa, NOVEPOETIN, a binding domain of erythropoietin or an erythropoietin-GFP fusion protein. In some embodiments the erythropoietin comprises an amino acid sequence that has at least 70% identity with SEQ ID NO: 164, or in other embodiments, at least 75%, 80%, 85%, 90% or 95% identity with SEQ ID NO: 164. In some embodiments, the erythropoietin comprises an amino acid sequence having from 1 to 20 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 164. Or in other embodiments, the erythropoietin comprises an amino acid sequence having from 1 to 15 or from 1 to 10 amino acid insertions, deletions, or substitutions (collectively) with respect to SEQ ID NO: 164.

By “reprogramming” is meant causing a change in the phenotype of a cell, by way of non-limiting example, causing a β-cell progenitor to differentiate into a mature β-cell, causing a fibroblast to dedifferentiate into a pluripotent stem cell, causing a keratinocyte to transdifferentiate into a cardiac stem cell, causing the telomeres of a cell to lengthen or causing the axon of a neuron to grow.

By “reprogramming factor” is meant a molecule that, when a cell is contacted with the molecule and/or the cell expresses the molecule, can, either alone or in combination with other molecules, cause reprogramming, by way of non-limiting example, Oct4 protein, Tert protein or erythropoietin.

By “germ cell” is meant a sperm cell or an egg cell.

By “pluripotent stem cell” is meant a cell that can differentiate into cells of all three germ layers (endoderm, mesoderm, and ectoderm) in vivo.

By “somatic cell” is meant a cell that is not a pluripotent stem cell or a germ cell, by way of non-limiting example, a skin cell.

By “hematopoietic cell” is meant a blood cell or a cell that can differentiate into a blood cell, by way of non-limiting example, a hematopoietic stem cell or a white blood cell.

By “cardiac cell” is meant a heart cell or a cell that can differentiate into a heart cell, by way of non-limiting example, a cardiac stem cell or a cardiomyocyte.

By “retinal cell” is meant a cell of the retina or a cell that can differentiate into a cell of the retina, by way of non-limiting example, a retinal pigmented epithelial cell.

By “skin cell” is meant a cell that is normally found in the skin, by way of non-limiting example, a fibroblast, a keratinocyte, a melanocyte, an adipocyte, a mesenchymal stem cell, an adipose stem cell or a blood cell.

By “immunosuppressant” is meant a substance that can suppress one or more aspects of an immune system, and that is not normally present in a mammal, by way of non-limiting example, B18R or dexamethasone.

By “single-strand break” is meant a region of single-stranded or double-stranded DNA in which one or more of the covalent bonds linking the nucleotides has been broken in one of the one or two strands.

By “double-strand break” is meant a region of double-stranded DNA in which one or more of the covalent bonds linking the nucleotides has been broken in each of the two strands.

By “nucleotide” is meant a nucleotide or a fragment or derivative thereof, by way of non-limiting example, a nucleobase, a nucleoside, a nucleotide-triphosphate, etc.

By “nucleoside” is meant a nucleotide or a fragment or derivative thereof, by way of non-limiting example, a nucleobase, a nucleoside, a nucleotide-triphosphate, etc.

By “gene editing” is meant altering the DNA sequence of a cell, by way of non-limiting example, by transfecting the cell with a protein that causes a mutation in the DNA of the cell or by transfecting the cell with a protein that causes a chemical change in the DNA of the cell.

By “gene-editing protein” is meant a protein that can, either alone or in combination with one or more other molecules, alter the DNA sequence of a cell, by way of non-limiting example, a nuclease, a transcription activator-like effector nuclease (TALEN), a zinc-finger nuclease, a meganuclease, a nickase, a clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein, a DNA-repair protein, a DNA-modification protein, a base-modification protein, a DNA methyltransferase, an protein that causes DNA demethylation, an enzyme for which DNA is a substrate or a natural or engineered variant, family-member, orthologue, domain, fragment or fusion construct thereof.

By “repair template” is meant a nucleic acid containing a region of at least about 70% homology with a sequence that is within 10 kb of a target site of a gene-editing protein.

By “repeat sequence” is meant an amino-acid sequence that is present in more than one copy in a protein, to within at least about 10% homology, by way of non-limiting example, a monomer repeat of a transcription activator-like effector.

By “DNA-binding domain” is meant a region of a molecule that is capable of binding to a DNA molecule, by way of non-limiting example, a protein domain comprising one or more zinc fingers, a protein domain comprising one or more transcription activator-like (TAL) effector repeat sequences or a binding pocket of a small molecule that is capable of binding to a DNA molecule.

By “binding site” is meant a nucleic-acid sequence that is capable of being recognized by a gene-editing protein, DNA-binding protein, DNA-binding domain or a biologically active fragment or variant thereof or a nucleic-acid sequence for which a gene-editing protein, DNA-binding protein, DNA-binding domain or a biologically active fragment or variant thereof has high affinity, by way of non-limiting example, an about 20-base-pair sequence of DNA in exon 1 of the human BIRC5 gene.

By “target” is meant a nucleic acid that contains a binding site.

Other definitions are set forth in U.S. application Ser. No. 13/465,490, U.S. Provisional Application No. 61/664,494, U.S. Provisional Application No. 61/721,302, International Application No. PCT/US12/67966, U.S. Provisional Application No. 61/785,404, U.S. Provisional Application No. 61/842,874, International Application No. PCT/US13/68118, U.S. Provisional Application No. 61/934,397, U.S. application Ser. No. 14/296,220, U.S. Provisional Application No. 62/038,608, U.S. Provisional Application No. 62/069,667, and International Application No. PCT/US2015/013949, the contents of which are hereby incorporated by reference in their entireties.

Selected Sequences

SEQ ID NO
Description

1
Stsl

2
Stsl-HA

3
Stsl-HA2

4
Stsl-UHA

5
Stsl-UHA2

6
Stsl-HF

7
Stsl-UHF

8
Oct4

9
Sox2

10
Klf4

11
c-Myc

12
BIRC5_exon1

13
BIRC5_exon2

14
BIRC5_exon3

15
BIRC5_exon4

16
BIRC5-1.1-L

17
BIRC5-1.1-R

18
BIRC5-1.2-L

19
BIRC5-1.2-R

20
BIRC5-1.3-L

21
BIRC5-1.3-R

22
BIRC5-2.1-L

23
BIRC5-2.1-R

24
BIRC5-2.2-L

25
BIRC5-2.2-R

26
BIRC5-3.1-L

27
BIRC5-3.1-R

28
CDK1

29
CDK2

30
CDK3

31
CDK4

32
CDK5

33
CDK6

34
BIRC5

35
HIF1A

36
RRM2

37
KRAS

38
EGFR

39
MYC

40
PKN3

41
KIF11

42
APC

43
BRCA1

44
BRCA2

45
TP53

46
APP

47
HTT

48
IAPP

49
MAPT

50
PRNP

51
SNCA

52
SOD1

53
Fokl

54
Repeat1

55
Repeat2

56
Repeat3

57
EO-GHGG-Fokl (“GHGG” disclosed as SEQ ID NO: 547)

58
GHGG-Fokl (“GHGG” disclosed as SEQ ID NO: 547)

59
EO-GHGG-Stsl (“GHGG” disclosed as SEQ ID NO: 547)

60
GHGG-Stsl (“GHGG” disclosed as SEQ ID NO: 547)

61
collagen alpha-1(I) chain preproprotein

62
collagen alpha-2(I) chain precursor

63
collagen alpha-1(II) chain isoform 1 precursor

64
collagen alpha-1(II) chain isoform 2 precursor

65
collagen alpha-1(III) chain preproprotein

66
collagen alpha-1(IV) chain preproprotein

67
collagen alpha-2(IV) chain preproprotein

68
collagen alpha-3(IV) chain precursor

69
collagen alpha-4(IV) chain precursor

70
collagen alpha-5(IV) chain isoform 1 precursor

71
collagen alpha-6(IV) chain isoform A precursor

72
collagen alpha-1(V) chain isoform 1 preproprotein

73
collagen alpha-2(V) chain preproprotein

74
collagen alpha-3(V) chain preproprotein

75
collagen alpha-1(VI) chain precursor

76
collagen alpha-2(VI) chain isoform 2C2 precursor

77
collagen alpha-3(VI) chain isoform 1 precursor

78
collagen alpha-1(VII) chain precursor

79
elastin isoform a precursor

80
elastin isoform b precursor

81
elastin isoform c precursor

82
elastin isoform d precursor

83
elastin isoform e precursor

84
elastin isoform f precursor

85
elastin isoform g precursor

86
elastin isoform h precursor

87
elastin isoform i precursor

88
elastin isoform j precursor

89
elastin isoform k precursor

90
elastin isoform l precursor

91
elastin isoform m precursor

92
protein-lysine 6-oxidase isoform 1 preproprotein

93
protein-lysine 6-oxidase isoform 2

94
telomerase reverse transcriptase isoform 1

95
telomerase reverse transcriptase isoform 2

96
fibronectin isoform 1 preproprotein

97
fibronectin isoform 3 preproprotein

98
fibronectin isoform 4 preproprotein

99
fibronectin isoform 5 preproprotein

100
fibronectin isoform 6 preproprotein

101
fibronectin isoform 7 preproprotein

102
vitronectin precursor

103
nidogen-1 precursor

104
laminin subunit alpha-1 precursor

105
insulin-like growth factor I isoform 1 preproprotein

106
fibroblast growth factor 1 isoform 1 precursor

107
fibroblast growth factor 2

108
transforming growth factor beta-1 precursor

109
transforming growth factor beta-2 isoform 1 precursor

110
transforming growth factor beta-2 isoform 2 precursor

111
actin, alpha skeletal muscle

112
actin, aortic smooth muscle

113
actin, cytoplasmic 1

114
actin, alpha cardiac muscle 1 proprotein

115
actin, cytoplasmic 2

116
actin, gamma-enteric smooth muscle isoform 1 precursor

117
actin, gamma-enteric smooth muscle isoform 2 precursor

118
granulocyte colony-stimulating factor isoform a precursor

119
granulocyte colony-stimulating factor isoform b precursor

120
granulocyte colony-stimulating factor isoform c precursor

121
granulocyte colony-stimulating factor isoform d precursor

122
platelet-derived growth factor subunit A isoform 1

preproprotein

123
platelet-derived growth factor subunit A isoform 2

preproprotein

124
platelet-derived growth factor subunit B isoform 1

preproprotein

125
platelet-derived growth factor subunit B isoform 2

preproprotein

126
platelet-derived growth factor C precursor

127
platelet-derived growth factor D isoform 1 precursor

128
platelet-derived growth factor D isoform 2 precursor

129
interstitial collagenase isoform 1 preproprotein

130
interstitial collagenase isoform 2

131
neutrophil collagenase preproprotein

132
stromelysin-2 preproprotein

133
macrophage metalloelastase preproprotein

134
fibrillin-1 precursor

135
fibrillin-2 precursor

136
lysyl oxidase homolog 1 preproprotein

137
lysyl oxidase homolog 2 precursor

138
lysyl oxidase homolog 3 isoform 1 precursor

139
lysyl oxidase homolog 3 isoform 2 precursor

140
lysyl oxidase homolog 3 isoform 3

141
lysyl oxidase homolog 4 precursor

142
microfibrillar-associated protein 2 isoform a precursor

143
microfibrillar-associated protein 2 isoform b precursor

144
microfibrillar-associated protein 5 precursor

145
disintegrin and metalloproteinase domain-containing

protein 17 preprotein

146
desmoglein-2 preproprotein

147
DNA polymerase eta isoform 1

148
DNA polymerase eta isoform 2

149
DNA polymerase eta isoform 3

150
ferrochelatase, mitochondrial isoform a precursor

151
ferrochelatase, mitochondrial isoform b precursor

152
filaggrin

153
hyaluronan synthase 1 isoform 1

154
hyaluronan synthase 1 isoform 2

155
hyaluronan synthase 2

156
hyaluronan synthase 3 isoform a

157
hyaluronan synthase 3 isoform b

158
proopiomelanocortin

159
plakophilin-1 isoform 1a

160
plakophilin-1 isoform 1b

161
retinol dehydrogenase 10

162
mitochondrial brown fat uncoupling protein 1

163
tyrosinase precursor

164
erythropoietin

165
epoetin alfa

166
darbepoetin alfa

167
NOVEPOETIN

168
NOVECRIT

This invention is further illustrated by the following non-limiting examples.

EXAMPLES
Example 1 RNA Synthesis

RNA encoding green fluorescent protein (“GFP”), NOVEPOETIN (“EPO”), elastin (“ELN”), tyrosinase (“TYR”), melanocortin-1-receptor (“MC1R”), HAS1, HAS2, HAS3, COL3A1, COL7A1, COL1A1, COL1A2, hTERT, Holly GFP, Fresno RFP, Blitzen Blue, RIBOSLICE gene-editing proteins, TALENs, Cas9, Oct4, Sox2, Klf4, c-Myc-2 (T58A), Lin28, IL2, IL6, IL15, IL22, BMP2, BMP7, BDNF, LIF, BMP6, IL15RA, FGF21, LIF, PTH, KRT5, KRT5-GFP, KRT14, KRT14-GFP, GDF15 and ESM1, and comprising various combinations of canonical and non-canonical nucleotides, was synthesized from DNA templates using the T7 High Yield RNA Synthesis Kit and the Vaccinia Capping System kit with mRNA Cap 2′-O-Methyltransferase (all from New England Biolabs, Inc.), according to the manufacturer's instructions and the present inventors' previously disclosed inventions (U.S. application Ser. No. 13/465,490 (now U.S. Pat. No. 8,497,124), International Application No. PCT/US12/67966, U.S. application Ser. No. 13/931,251, International Application No. PCT/US13/68118, and International Application No. PCT/US2015/013949, the contents of all of which are hereby incorporated by reference in their entirety) (Table 4). The RNA was then diluted with nuclease-free water to between 100 ng/μL and 2000 ng/μL. For certain experiments, an RNase inhibitor (Superase⋅In, Life Technologies Corporation) was added at a concentration of 1 μL/100 μg of RNA. RNA solutions were stored at room temperature, 4° C., −20° C. or −80° C. For reprogramming experiments, RNA encoding Oct4, Sox2, Klf4, c-Myc-2 (T58A), and Lin28 was mixed at a molar ratio of 3:1:1:1:1.

TABLE 4

RNA Synthesis

Reaction
ivT

Template
Nucleotides
Volume/μL
Yield/μg

ELN
A, 0.5 7dG, 0.4 5mU, 5mC
20
34.1

ELN
A, 0.5 7dG, 0.4 5mU, 5mC
20
67.6

GFP
A, 0.5 7dG, 0.4 5mU, 5mC
10
60.5

GFP
A, 0.5 7dG, 0.4 5mU, 5hmC
10
25.5

GFP
A, G, U, 5hmC
10
58.3

GFP
A, 0.5 7dG, U, 5hmC
10
47.3

GFP
A, 0.5 7dG, 0.4 5mU, 5cC
10
33.8

GFP
A, G, U, 5hmC
15
30.3

GFP
A, G, U, 5hmC
15
44.6

GFP
A, G, U, 5hmC
15
24.7

TYR
A, G, U, 5hmC
15
45.4

MC1R
A, G, U, 5hmC
15
47.5

TYR
A, G, U, C
20
67.0

TYR
A, G, psU, C
20
93.7

TYR
A, G, 0.4 5mU, C
20
85.7

TYR
A, G, U, 5mC
20
73.4

TYR
A, G, U, 5hmC
20
72.7

TYR
A, 0.5 7dG, U, C
20
62.7

TYR
A, G, psU, 5mC
20
116.3

TYR
A, G, psU, 5hmC
20
102.4

TYR
A, 0.5 7dG, psU, C
20
87.3

TYR
A, G, 0.4 5mU, 5mC
20
106.5

TYR
A, G, 0.4 5mU, 5hmC
20
85.0

TYR
A, 0.5 7dG, 0.4 5mU, C
20
70.9

TYR
A, 0.5 7dG, U, 5mC
20
88.5

TYR
A, 0.5 7dG, U, 5hmC
20
59.1

TYR
A, 0.5 7dG, psU, 5mC
20
7.8

TYR
A, 0.5 7dG, psU, 5hmC
20
98.0

TYR
A, 0.5 7dG, 0.4 5mU, 5mC
20
106.5

TYR
A, 0.5 7dG, 0.4 5mU, 5hmC
20
82.3

HAS1
A, G, U, 5hmC
20
178.4

HAS2
A, G, U, 5hmC
20
59.3

HAS3
A, G, U, 5hmC
20
102.6

TYR
A, G, 0.4 5mU, 5hmC
100
377.3

COL3A1
A, G, 0.4 5mU, 5hmC
20
108.3

COL7A1
A, G, 0.4 5mU, 5hmC
20
94.6

COL1A1 (20 μL)
A, G, 0.4 5mU, 5hmC
20
114.0

COL1A2 (10 μL)
A, G, 0.4 5mU, 5hmC
10
31.3

TYR
A, G, 0.4 5mU, 5hmC
100
249.9

GFP
A, G, 0.4 5mU, 5hmC
100
264.0

hTERT
A, G, 0.4 5mU, 5hmC
100
349.2

GFP
A, G, U, 5hC
20
81.7

GFP
A, G, U, 0.5 5hC
20
65.4

GFP
A, sG, U, C
20
34.7

GFP
A, 0.5 sG, U,C
20
47.5

GFP
A, G, 5hmU, C
20
22.1

GFP
A, G, 0.5 5hmU, C
20
28.4

GFP
A, G, 5cU, C
20
24.4

GFP
A, G, 0.5 5cU, C
20
28.4

GFP
A, G, 5moU, C
20
39.2

GFP
A, G, 0.5 5moU, C
20
34.2

GFP
A, G, U, C
20
42.0

GFP
A, G, 5moU, C
20
53.8

GFP
A, G, 5moU, 5hmC
20
101.5

GFP
A, G, 0.4 5mU, 0.6 5moU, C
20
98.6

GFP
A, G, 0.4 5mU, C
20
99.6

GFP
A, G, U, 5mC
20
106.1

GFP
A, G, U, C
20
85.7

GFP
A, G, 5moU, C
100
398.4

hTERT
A, G, 5moU, C
20
82.6

COL7A1
A, G, 5moU, C
20
34.9

COL7A1
A, G, 5moU, C
100
342.0

Holly GFP
A, G, 5moU, C
20
36.7

Fresno RFP
A, G, 5moU, C
20
72.0

Blitzen Blue
A, G, 5moU, C
20
30.3

hTERT
A, G, 5moU, C
20
49.6

Cas9
A, G, 5moU, C
20
31.6

EPO
A, G, U, C
20
101.0

EPO
A, G, 5moU, C
20
52.9

EPO
A, G, psU, C
20
106.0

COL7A1
A, G, 5moU, C
20
80.2

Oct4 (SEQ ID NO: 8)
A, G, 5moU, C
300
1925.5

Sox2 (SEQ ID NO: 9)
A, G, 5moU, C
100
641.8

Klf4 (SEQ ID NO: 10)
A, G, 5moU, C
100
739.0

c-Myc-2 (T58A)
A, G, 5moU, C
100
574.0

Lin28
A, G, 5moU, C
100
556.0

IL2
A, G, 5moU, C
20
62.4

IL6
A, G, 5moU, C
20
22.2

IL15
A, G, 5moU, C
20
50.4

IL22
A, G, 5moU, C
20
63.6

BMP2
A, G, 5moU, C
20
83.2

BDNF
A, G, 5moU, C
20
45.0

LIF
A, G, 5moU, C
20
54.0

BMP6
A, G, 5moU, C
20
92.2

IL15RA
A, G, 5moU, C
20
91.4

FGF21
A, G, 5moU, C
20
79.2

GFP
A, G, 5moU, C
40
181.0

IL2
A, G, 5moU, C
30
99.4

IL6
A, G, 5moU, C
30
31.2

IL15
A, G, 5moU, C
30
89.8

IL22
A, G, 5moU, C
30
104.0

BDNF
A, G, 5moU, C
30
95.9

BMP2
A, G, 5moU, C
30
112.0

LIF
A, G, 5moU, C
30
116.0

PTH
A, G, 5moU, C
30
88.4

EPO
A, G, 5moU, C
30
83.3

KRT5
A, G, 5moU, C
15
66.6

KRT5-GFP
A, G, 5moU, C
15
81.1

KRT14
A, G, 5moU, C
15
75.1

KRT14-GFP
A, G, 5moU, C
15
90.4

GDF15
A, G, 5moU, C
15
71.1

A1AT TALEN L
A, G, 5moU, C
15
56.4

A1AT TALEN R
A, G, 5moU, C
15
57.3

A1AT RIBOSLICE L_A
A, G, 5moU, C
15
74.3

A1AT RIBOSLICE L_B
A, G, 5moU, C
15
56.4

A1AT RIBOSLICE R_A
A, G, 5moU, C
15
60.3

A1AT RIBOSLICE R_B
A, G, 5moU, C
15
35.7

COL7A1 exon 73 TALEN L
A, G, 5moU, C
15
86.48

COL7A1 exon 73 TALEN R
A, G, 5moU, C
15
83.66

COL7A1 exon 73 rs3L 50A
A, G, 5moU, C
15
103.4

COL7A1 exon 73 rs3L 50B
A, G, 5moU, C
15
112.8

COL7A1 exon 73 rs3R 50A
A, G, 5moU, C
15
81.404

COL7A1 exon 73 rs3R 50B
A, G, 5moU, C
15
78.02

COL7A1 exon 73 TALEN L
A, G, 5moU, C
15
88.924

EA

COL7A1 exon 73 TALEN L
A, G, 5moU, C
15
75.2

Het

COL7A1 exon 73 TALEN R
A, G, 5moU, C
15
86.48

EA

COL7A1 exon 73 TALEN R
A, G, 5moU, C
15
62.98

Het

COL7A1 exon 73 TALEN L
A, G, 5moU, C
15
82.7

EA/Het

COL7A1 exon 73 TALEN R
A, G, 5moU, C
15
69.7

EA/Het

HBB exon 1 TALEN L
A, G, 5moU, C
15
112.8

HBB exon 1 TALEN R
A, G, 5moU, C
15
108.1

PD-1 exon 1 TALEN L
A, G, 5moU, C
15
95.88

PD-1 exon 1 TALEN R
A, G, 5moU, C
15
101.52

ESM1 - transcript variant 1
A, G, 5moU, C
15
61

ESM1 - transcript variant 2
A, G, 5moU, C
15
66

“A” refers to adenosine-5′-triphosphate, “G” refers to guanosine-5′-triphosphate, “U” refers to uridine-5′-triphosphate, “C” refers to cytidine-5′-triphosphate, “7dG” refers to 7-deazaguanosine-5′-triphosphate, “sG” refers to thienoguanosine-5′-triphosphate, “5mC” refers to 5-methylcytidine-5′-triphosphate, “5hmC” refers to 5-hydroxymethylcytidine-5′-triphosphate, “5cC” refers to 5-carboxycytidine-5′-triphosphate, “5fC” refers to 5-formylcytidine-5′-triphosphate, “5hC” refers to 5-hydroxycytidine-5′-triphosphate, “psU” refers to 5-pseudouridine-5′-triphosphate, “5mU” refers to 5-methyluridine-5′-triphosphate, “5hmU” refers to 5-hydroxymethyluridine-5′-triphosphate, “5cU” refers to 5-carboxyuridine-5′-triphosphate, and “5moU” refers to 5-methoxyuridine-5′-triphosphate.

Example 2 Preparation of RNA-Transfection-Reagent Complexes

For each microgram of RNA, 1 μg RNA and 1 μL transfection reagent (LIPOFECTAMINE 3000, Life Technologies Corporation) were first diluted separately in complexation medium (Opti-MEM, Life Technologies Corporation or DMEM/F12+10 μg/mL insulin+5.5 μg/mL transferrin+6.7 ng/mL sodium selenite+2 μg/mL ethanolamine) to a total volume of between 5 μL and 100 μL each. Diluted RNA and transfection reagent were then mixed and incubated for 10 min at room temperature, according to the transfection reagent-manufacturer's instructions.

Example 3 Transfection of Cells with Synthetic RNA

Complexes were prepared according to Example 2, and were then added directly to cells in culture. For transfection in 6-well plates, between 10 μL and 250 μL of complexes were added to each well of the 6-well plate, which already contained 2 mL of transfection medium per well. Plates were shaken gently to distribute the complexes throughout the well. Cells were incubated with complexes for 4 hours to overnight, before replacing the medium with fresh transfection medium (2 mL/well). Alternatively, the medium was not replaced. Volumes were scaled for transfection in 24-well and 96-well plates.

Example 4 Toxicity of and Protein Translation from Synthetic RNA Containing Non-Canonical Nucleotides

Primary human fibroblasts were transfected according to Example 2, using RNA synthesized according to Example 1. Cells were fixed and stained 20-24h after transfection using an antibody against Oct4. The relative toxicity of the RNA was determined by assessing cell density at the time of fixation.

Example 5 Delivery of Synthetic RNA to the Skin

The complexation reaction shown in Table 5 was prepared using RNA encoding green fluorescent protein (GFP) or collagen, type VII, alpha1 (COL7), synthesized according to Example 1. The concentration of the RNA stock solution was 500 μg/mL.

TABLE 5

RNA Complexation Reaction

Volume

RNA solution tube

GFP or COL7 RNA
8 μL

FactorPlex ™ complexation buffer
42 μL

Transfection reagent solution tube

LIPOFECTAMINE 3000 (LIFE TECHNOLOGIES)
4 μL

FactorPlex ™ complexation buffer
46 μL

Each tube was mixed by pipetting, and the transfection reagent solution tube was incubated for 30s at room temperature. The transfection reagent solution was then transferred to the RNA solution, and the contents were mixed by rapidly pipetting up and down 10 times. Following a 10 min incubation, dilutions were prepared according to

TABLE 6

Injection Solutions

Complexation
FactorPlex ™
RNA

Site
RNA
Volume
Volume
amount

1
GFP
7.5 μL
22.5 μL
0.3 μg

2
GFP
15 μL
15 μL
0.6 μg

3
GFP
30 μL
0 μL
1.2 μg

4
COL7
30 μL
0 μL
1.2 μg

For each injection, the corresponding solution was drawn into a 3 cc insulin syringe with an 8 mm, 31 gauge needle (Becton, Dickinson and Company, Part Number: 328291) and air bubbles were removed. A clear field was selected on the left forearm of a healthy 33 year-old male human subject, and was disinfected with 70% isopropanol and allowed to dry. The needle was positioned at an angle of approximately 10° to the anterior (palmar) forearm with bevel facing up, and was inserted until the bevel was just covered. 30 μL of the RNA solution was injected intradermally over the course of approximately 10 sec. A distinct wheal appeared during the injection process. The needle was withdrawn, the wheal remained for approximately 1 minute, and no fluid escaped from the injection site. A total of 4 injections were performed according to Table 6, and all of the injections were performed between 11 and 28 minutes following the preparation of the RNA complexation reaction. No swelling, redness, or soreness occurred as a result of the injections. A small amount of bleeding occurred when the needle was removed from sites 2 and 4, resulting in the appearance of a small red spot at these sites.

The injection sites were imaged according to the schedule of Table 7, and every 24 hours thereafter for 6 days. Fluorescence images were acquired using an inverted microscope (Nikon Eclipse TS100) equipped with an EXFO X-Cite™ 120 fluorescence illumination system and the filter sets shown in Table 7. Fluorescence images were captured using a Sony NEX-7 digital camera (FIGS. 9-12).

TABLE 7

Measurement Parameters

Site
Time
Image Type
Exposure Time

All
0
h
Brightfield
Automatic

1
0
h
FITC
1/10
s

2
0
h
FITC
1/10
s

3
0
h
FITC
1/10
s

4
0
h
FITC
1/10
s

1
12
h
FITC
1/10
s

2
12
h
FITC
1/10
s

3
12
h
FITC
1/10
s

4
12
h
FITC
1/10
s

1
12
h
FITC
1/20
s

2
12
h
FITC
1/20
s

3
12
h
FITC
1/20
s

4
12
h
FITC
1/20
s

All
24
h
Brightfield
Automatic

1
24
h
FITC
1/20
s

2
24
h
FITC
1/20
s

3
24
h
FITC
1/20
s

4
24
h
FITC
1/20
s

1
24
h
Cy3.5
⅕
s

2
24
h
Cy3.5
⅕
s

3
24
h
Cy3.5
⅕
s

4
24
h
Cy3.5
⅕
s

1
24
h
Cy3
⅕
s

2
24
h
Cy3
⅕
s

3
24
h
Cy3
⅕
s

4
24
h
Cy3
⅕
s

1
36
h
FITC
1/20
s

2
36
h
FITC
1/20
s

3
36
h
FITC
1/20
s

4
36
h
FITC
1/20
s

1
48
h
FITC
1/20
s

2
48
h
FITC
1/20
s

3
48
h
FITC
1/20
s

4
48
h
FITC
1/20
s

An independent experiment was carried out using the 1.2 μg dose of GFP RNA, with similar results (FIG. 12).

TABLE 8

Filter Sets

Image Type
Filter Set

Cy3
Chroma SP102V2

Cy3.5
Chroma SP103V2

FITC
Chroma SP101

Example 6 Transfection of Human Keratinocytes with RNA Encoding NOVEPOETIN

RNA encoding NOVEPOETIN was synthesized according to Example 1 with three nucleotide combinations: 1) U, G, U, C, 2) U, G, 5moU, C, and 3) U, G, psU, C. Sub-confluent layers of primary human keratinocytes cultured in EpiLife medium were transfected in wells of a 6 well plate according to Example 3 with 1 μg of RNA per well. 12, 24, 36 and 48h following transfection, 0.5 mL of medium was removed and 0.5 mL of fresh EpiLife medium was added to the plate. After the final medium sampling, the cells were harvested by trypsinization, and total RNA was isolated using the RNeasy mini kit (Qiagen). Genomic DNA was digested using DNase I and RNA was purified. Expression of interferon-β and GAPDH was measured by RT-PCR (FIG. 5).

Example 7 Transfection of Human Cells with RNA Encoding hTERT

RNA encoding human telomerase reverse transcriptase (hTERT) was synthesized according to Example 1 with the following nucleotides: U, G, 5moU, C. A sub-confluent layer of primary human dermal fibroblasts cultured in DMEM+10% FBS were transfected in wells of a 24 well plate according to Example 3 with 0.25 μg of RNA per well. 12h after transfection, cells were fixed and stained using a 1:50 dilution of rabbit anti-hTERT antibody (Millipore, Part Number: MABE14) (FIG. 16).

Example 8 High-Efficiency Gene Editing by Repeated Transfection with RIBOSLICE

Primary human fibroblasts were plated in 6-well plates coated with recombinant human fibronectin and recombinant human vitronectin (each diluted in DMEM/F12 to a concentration of 1 μg/mL, 1 mL/well, and incubated at room temperature for 1h) at a density of 10,000 cells/well in transfection medium. The following day, the cells were transfected as in Example 2 with RNA synthesized according to Example 1. The following day cells in one of the wells were transfected a second time. Two days after the second transfection, the efficiency of gene editing was measured using a mutation-specific nuclease assay.

Example 9 Transfection of Cells with Synthetic RNA Containing Non-Canonical Nucleotides and DNA Encoding a Repair Template

For transfection in 6-well plates, 1 μg RNA encoding gene-editing proteins targeting exon 16 of the human APP gene, 1 μg single-stranded repair template DNA containing a PstI restriction site that was not present in the target cells, and 6 μL transfection reagent (LIPOFECTAMINE RNAiMAX, Life Technologies Corporation) were first diluted separately in complexation medium (Opti-MEM, Life Technologies Corporation) to a total volume of 120 μL. Diluted RNA, repair template, and transfection reagent were then mixed and incubated for 15 min at room temperature, according to the transfection reagent-manufacturer's instructions. Complexes were added to cells in culture. Approximately 120 μL of complexes were added to each well of a 6-well plate, which already contained 2 mL of transfection medium per well. Plates were shaken gently to distribute the complexes throughout the well. Cells were incubated with complexes for 4 hours to overnight, before replacing the medium with fresh transfection medium (2 mL/well). The next day, the medium was changed to DMEM+10% FBS. Two days after transfection, genomic DNA was isolated and purified. A region within the APP gene was amplified by PCR, and the amplified product was digested with PstI and analyzed by gel electrophoresis.

Example 10 In Vivo RIBOSLICE Safety Study

40 female NCr nu/nu mice were injected subcutaneously with 5×10⁶MDA-MB-231 tumor cells in 50% Matrigel (BD Biosciences). Cell injection volume was 0.2 mL/mouse. The age of the mice at the start of the study was 8 to 12 weeks. A pair match was conducted, and animals were divided into 4 groups of 10 animals each when the tumors reached an average size of 100-150 mm³, and treatment was begun. Body weight was measured every day for the first 5 days, and then biweekly to the end of the study. Treatment consisted of RIBOSLICE BIRC5-1.2 complexed with a vehicle (LIPOFECTAMINE 2000, Life Technologies Corporation). To prepare the dosing solution for each group, 308 μL of complexation buffer (Opti-MEM, Life Technologies Corporation) was pipetted into each of two sterile, RNase-free 1.5 mL tubes. 22 μL of RIBOSLICE BIRC5-1.2 (500 ng/μL) was added to one of the two tubes, and the contents of the tube were mixed by pipetting. 22 μL of vehicle was added to the second tube. The contents of the second tube were mixed, and then transferred to the first tube, and mixed with the contents of the first tube by pipetting to form complexes. Complexes were incubated at room temperature for 10 min. During the incubation, syringes were loaded. Animals were injected either intravenously or intratumorally with a total dose of 1 μg RNA/animal in 60 μL total volume/animal. A total of 5 treatments were given, with injections performed every other day. Doses were not adjusted for body weight. Animals were followed for 17 days. No significant reduction in mean body weight was observed, demonstrating the in vivo safety of RIBOSLICE gene-editing RNA.

Example 11 Screening of Reagents for Delivery of Nucleic Acids to Cells

Delivery reagents including polyethyleneimine (PEI), various commercial lipid-based transfection reagents, a peptide-based transfection reagent (N-TER, Sigma-Aldrich Co. LLC.), and several lipid-based and sterol-based delivery reagents were screened for transfection efficiency and toxicity in vitro. Delivery reagents were complexed with RIBOSLICE BIRC5-1.2, and complexes were delivered to HeLa cells in culture. Toxicity was assessed by analyzing cell density 24 h after transfection. Transfection efficiency was assessed by analyzing morphological changes. The tested reagents exhibited a wide range of toxicities and transfection efficiencies. Reagents containing a higher proportion of ester bonds exhibited lower toxicities than reagents containing a lower proportion of ester bonds or no ester bonds.

Example 12 High-Concentration Liposomal RIBOSLICE

High-Concentration Liposomal RIBOSLICE was prepared by mixing 1 μg RNA at 500 ng/μL with 3 μL of complexation medium (Opti-MEM, Life Technologies Corporation), and 2.5 μL of transfection reagent (LIPOFECTAMINE 2000, Life Technologies Corporation) per μg of RNA with 2.5 μL of complexation medium. Diluted RNA and transfection reagent were then mixed and incubated for 10 min at room temperature to form High-Concentration Liposomal RIBOSLICE. Alternatively, a transfection reagent containing DOSPA or DOSPER is used.

Example 13 In Vivo RIBOSLICE Efficacy Study—Subcutaneous Glioma Model

40 female NCr nu/nu mice were injected subcutaneously with 1×10⁷U-251 tumor cells. Cell injection volume was 0.2 mL/mouse. The age of the mice at the start of the study was 8 to 12 weeks. A pair match was conducted, and animals were divided into 4 groups of 10 animals each when the tumors reached an average size of 35-50 mm³, and treatment was begun. Body weight was measured every day for the first 5 days, and then biweekly to the end of the study. Caliper measurements were made biweekly, and tumor size was calculated. Treatment consisted of RIBOSLICE BIRC5-2.1 complexed with a vehicle (LIPOFECTAMINE 2000, Life Technologies Corporation). To prepare the dosing solution, 294 μL of complexation buffer (Opti-MEM, Life Technologies Corporation) was pipetted into a tube containing 196 μL of RIBOSLICE BIRC5-1.2 (500 ng/μL), and the contents of the tube were mixed by pipetting. 245 μL of complexation buffer was pipetted into a tube containing 245 μL of vehicle. The contents of the second tube were mixed, and then transferred to the first tube, and mixed with the contents of the first tube by pipetting to form complexes. Complexes were incubated at room temperature for 10 min. During the incubation, syringes were loaded. Animals were injected intratumorally with a total dose of either 2 μg or 5 μg RNA/animal in either 20 μL or 50 μL total volume/animal. A total of 5 treatments were given, with injections performed every other day. Doses were not adjusted for body weight. Animals were followed for 25 days.

Example 14 Liposome Formulation and Nucleic-Acid Encapsulation

Liposomes are prepared using the following formulation: 3.2 mg/mL N-(carbonyl-ethoxypolyethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (MPEG2000-DSPE), 9.6 mg/mL fully hydrogenated phosphatidylcholine, 3.2 mg/mL cholesterol, 2 mg/mL ammonium sulfate, and histidine as a buffer. pH is controlled using sodium hydroxide and isotonicity is maintained using sucrose. To form liposomes, lipids are mixed in an organic solvent, dried, hydrated with agitation, and sized by extrusion through a polycarbonate filter with a mean pore size of 800 nm. Nucleic acids are encapsulated by combining 10 μg of the liposome formulation per 1 μg of nucleic acid and incubating at room temperature for 5 minutes.

Example 15 Folate-Targeted Liposome Formulation

Liposomes are prepared using the following formulation: 3.2 mg/mL N-(carbonyl-ethoxypolyethylene glycol 2000)-1,2-distearoyl-sn-glycero-3-phosphoethanolamine (MPEG2000-DSPE), 9.6 mg/mL fully hydrogenated phosphatidylcholine, 3.2 mg/mL cholesterol, 2 mg/mL ammonium sulfate, and histidine as a buffer, with 0.27 mg/mL 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[folate(polyethylene glycol)-5000] (FA-MPEG5000-DSPE) added to the lipid mixture. pH is controlled using sodium hydroxide and isotonicity is maintained using sucrose. To form liposomes, lipids are mixed in an organic solvent, dried, hydrated with agitation, and sized by extrusion through a polycarbonate filter with a mean pore size of 800 nm. Nucleic acids are encapsulated by combining 10 μg of the liposome formulation per 1 μg of nucleic acid and incubating at room temperature for 5 minutes.

Example 16 Therapy Comprising Liposomal Protein-Encoding RNA

Liposomes encapsulating synthetic RNA encoding a therapeutic protein, synthesized according to Example 1, are prepared according to Example 14 or Example 15. The liposomes are administered by injection or intravenous infusion.

Example 17 Generation of Elastin ivT-RNA Template

Total RNA was extracted from neonatal human dermal fibroblasts using the RNeasy mini kit (QIAGEN GmbH), according to the manufacturer's instructions. cDNA encoding human elastin was prepared using MonsterScript™ Reverse Transcriptase (Epicentre Biotechnologies) and the primer: AAAAAAACCGGT TCATTTTCTCTTCCGGCCAC (SEQ ID NO: 483). An in vitro transcription (ivT) template was prepared from the cDNA by PCR amplification of the elastin coding sequence (CDS) using the primers: F: AAAAAAGCTAGCATGGCGGGTCTGACG (SEQ ID NO: 484), and R: AAAAAAACCGGTTCATTTTCTCTTCCGGCCAC (SEQ ID NO: 485). The PCR product was then purified using agarose gel electrophoresis and the QIAquick Gel Extraction Kit (QIAGEN GmbH) and was cloned into a vector containing the human beta globin (HBB) 5′ and 3′ untranslated regions and a strong Kozak sequence. The vector was amplified, purified, and linearized prior to RNA synthesis.

Example 18 Generation of Tyrosinase ivT-RNA Template

Total RNA was extracted from human epidermal melanocytes using the RNeasy mini kit (QIAGEN GmbH), according to the manufacturer's instructions. cDNA encoding human tyrosinase was prepared using MonsterScript™ Reverse Transcriptase (Epicentre Biotechnologies). An in vitro transcription (ivT) template was prepared from the cDNA by PCR amplification of the tyrosinase coding sequence (CDS). The PCR product was then purified using agarose gel electrophoresis and the QIAquick Gel Extraction Kit (QIAGEN GmbH) and was cloned into a vector containing the human beta globin (HBB) 5′ and 3′ untranslated regions and a strong Kozak sequence. The vector was amplified, purified, and linearized prior to RNA synthesis.

Example 19 Synthesis of Tyrosinase RNA

RNA encoding human tyrosinase was synthesized according to Example 1, using the DNA template of Example 18 and the T7 High Yield RNA Synthesis Kit (New England Biolabs, Inc.), according to the manufacturer's instructions (Table 4). Samples of the RNA were analyzed by agarose gel electrophoresis to assess the quality of the RNA. The RNA was then diluted to 1 μg/μL. The RNA solution was stored at 4C.

Example 20 Increasing Melanin Production in Skin by Transdermal Injection Via Syringe of RNA Encoding Tyrosinase

The RNA of Example 19 was loaded into a syringe and delivered by intradermal injection to the ventral forearm of a healthy 33 year-old male patient over the course of approximately 30 seconds.

Example 21 Increasing Melanin Production in Skin by Combined Delivery of RNA Encoding Tyrosinase and Electroporation

The area of skin treated in Example 20 was exposed to electrical pulses of between 10V and 155V and between approximately 10 milliseconds and approximately 1 second using a two-electrode array electrically connected to a capacitor. The patient reported a tingling sensation at all voltages and penetration depths. The treated area became darker after 24-48 hours. The experiment was repeated several times, with similar results.

Example 22 Increasing Melanin Production in Skin by Topical or Intradermal Application of RNA Encoding Tyrosinase

The RNA of Example 19 or the liposomes of Example 16 are applied directly to the skin, with or without disruption of the stratum corneum or injected intradermally or delivered by injection to the epidermis using a dose of one microgram or less per square centimeter. Optionally, an electric field is applied as in Example 21 or using a surface-contact patch to enhance delivery of the RNA.

Example 23 Increasing Elastin Production in Skin by Transdermal Delivery of RNA Encoding Elastin

RNA encoding elastin was prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 24 Increasing Collagen Production in Skin by Transdermal Delivery of RNA Encoding Collagen

RNA encoding collagen was prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 25 Anemia Therapy Comprising Delivery of RNA Encoding NOVEPOETIN

RNA encoding NOVEPOETIN was prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 26 Increasing Production of Actin in Skeletal Muscle by Intramuscular Delivery of RNA Encoding Actin

RNA encoding actin is prepared according to Example 1. The RNA is delivered to the patient via intramuscular injection with or without the use of an electric field as in Example 20, 21, or 22.

Example 27 Wound Healing Treatment

RNA encoding basic fibroblast growth factor or IL22 is prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 28 Anti-Scarring Treatment

RNA encoding collagenase is prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 29 Production of Botulinum Toxin

RNA encoding botulinum toxin is prepared according to Example 1. The RNA is delivered as in Example 20, 21, or 22.

Example 30 Increasing Collagen Production in Skin Cells by Transfection with RNA Encoding Collagen I

RNA comprising the coding sequence of the human COL1A1 gene was synthesized according to Example 1. Primary human dermal fibroblasts were plated in wells of a 24-well plate, and were transfected according to Example 2. Between 24 and 72 hours after transfection, the cells were fixed and stained using an antibody targeting collagen I. Many extracellular deposits of collagen were visible in the transfected wells.

Example 31 Increasing Collagen Production in Skin Cells by Transfection with RNA Encoding Collagen VII

RNA comprising the coding sequence of the human COL7 gene was synthesized according to Example 1. Primary human dermal fibroblasts were plated in wells of a 24-well plate, and were transfected according to Example 2. Between 24 and 72 hours after transfection, the cells were fixed and stained using an antibody targeting collagen VII. Transfected cells exhibited high levels of collagen VII, compared to an un-transfected control.

Example 32 Increasing Collagen Production in Skin by Transdermal Injection Via Syringe of RNA Encoding Collagen I or Collagen VII

RNA comprising the coding sequence of the human COL1A1 gene or the human COL7 gene was synthesized according to Example 1. The RNA is loaded into a syringe and delivered to the dermis of a patient over the course of approximately 30 seconds or as in Example 20, 21, or 22.

Example 33 Increasing Collagen Production in Skin by Combined Delivery of RNA Encoding Collagen I or Collagen VII and Electroporation

The area of skin treated in Example 32 is exposed to electrical pulses of between 10V and 155V and between approximately 50 microseconds and approximately 1 second using a multi-electrode array electrically connected to a power source.

Example 34 Storage and Stability of Synthetic RNA Complexes

A complexation reaction using RNA encoding GFP was prepared according to Example 5. Following the 10 min incubation, the complexation reaction was divided into three equal parts, one of which was diluted 1:10 in FactorPlex™ complexation medium, one of which was diluted 1:10 in sterile, nuclease-free water, and one of which was left undiluted. Each of the three parts was then further divided into four equal parts, one of which was applied to primary human dermal fibroblasts according to Example 3, one of which was left at room temperature for six hours before applying to primary human dermal fibroblasts according to Example 3, one of which was placed at 4° C. for six hours before applying to primary human dermal fibroblasts according to Example 3, and one of which was snap frozen in liquid nitrogen and placed at −80° C. for six hours before applying to primary human dermal fibroblasts according to Example 3. The cells were imaged using a fluorescence microscope approximately 24 hours after the first transfection (FIG. 17). All wells contained GFP-positive cells, demonstrating that the synthetic RNA complexes were stable and maintained activity in all of the storage conditions tested.

Example 35: In Vivo Analysis of NOVECRIT

RNA encoding NOVEPOETIN was synthesized according to Example 1 with the nucleotide combination A, G, 5moU, C. In this Example, NOVECRIT was formulated with a lipid delivery vehicle, specifically LIPOFECTAMINE 3000. In this Example, NOVECRIT encoded NOVEPOETIN, a novel, high-stability erythropoiesis-stimulating agent.

A 15-day maximum tolerated dose (MTD) study was performed to evaluate safety (rat, n=62). In vivo toxicology and biodistribution, as well as pharmacodynamics and dose response and therapeutic effect, specifically on erythropoiesis, were evaluated. Furthermore, the immune response was monitored by analysis of cytokines in treated animals.

Sixty-nine, naïve, 8 week old male Sprague-Dawley rats (Rattus norvegicus) weighing 253 to 274 grams at receipt were used (HARLAN LABORATORIES). Animals were acclimated to the study room for at least seven days prior to dosing. On Day −2 all animals were shaved in the dorsal lumbar area and four intradermal sites (upper left, upper right, lower right and lower left) were designated on the dorsal back using a permanent marker. Sixty-four animals were randomly assigned to four treatment groups, with the remaining five animals serving as spares. Two animals were dropped from the study because of incomplete dosing.

The following study design was used (total dose volumes (μL) were constant):

Dose

Dose

Level
Dose
Conc.
Vol.

Group
(μg)
Route
(μg/mL)
(μL)
Number of Males

1
0
Intra-
0
50
4^a+ 2^b+ 2^h

dermal

2
0.25
Intra-
5.0
50
4^a+ 2^b+ 2^c+ 2^d+

dermal

2^e+ 2^f+ 2^g+ 2^h

3
1.0
Intra-
20
50
4^a+ 2^b+ 2^c+ 2^d+

dermal

2^e+ 2^f+ 2^g+ 2^h

4
4 × 1.0 μg
Intra-
20
50
4^a+ 2^b+ 3^c+ 3^d+

(4.0 μg total)
dermal

2^e+ 2^f+ 2^g+ 2^h

ID: ^aToxicology animals necropsy on Day 15, TK animals with terminal tissue collections on Days 6^band 24^chours postdose, and on Days 3^d, 4^e, 6^f, 8^g, and 15^h

Blood was collected from the vena cava from anesthetized animals prior to necropsy or terminal tissue collection. Whenever possible, blood was collected via a single draw and then divided appropriately. Blood specimens for toxicokinetic analysis were collected from two animals per time point for Group 1 at 6 hours postdose and on Day 15. For Groups 2-4, blood specimens were collected at 6 and 24 hours postdose, and on Days 3, 4, 6, 8, and 15.

Blood specimens for hematology assessment were collected from all toxicology animals on Day 15 following an overnight fast, and all TK animals scheduled for terminal tissue collections at 6 and 24 hours postdose and on Days 8 and 15. Whole blood (1.3 mL) was deposited into K2EDTA tubes and analyzed using an Advia 120 automated analyzer.

Blood specimens for coagulation assessment were collected from all toxicology animals on Day 15 following an overnight fast. Coagulation specimens (1.8 mL) were collected into 3.2% sodium citrate tubes, processed according to standard procedures and analyzed using a STA Compact automated analyzer.

Blood specimens for serum chemistry assessments were collected from all toxicology animals on Day 15 following an overnight fast. Serum chemistry specimens (1 mL) were collected into serum separator tubes, processed according to standard procedures and analyzed using an AU680 analyzer.

Blood specimens for cytokine analysis were collected from animals scheduled for terminal tissue collection at 5 and 24 hours postdose and Day 8. Cytokine specimens (1 mL) were collected into K2EDTA tubes and processed to plasma according to standard procedures. TNFα, IL-6, and IFNα cytokine levels in plasma samples were studied. The study measured the production of TNFα, IL-6, and IFNα cytokines in plasma samples following dosing with the test article on Day 1 (6 hours post-dose), Day 2 (24 hours post-dose) and Day 8.

Study Outline for Cytokine Analysis Samples:

Dose

NOVECRIT
Concen-
Dose
Number

Dose Level
tration
Volume
of Rats

Group
(μg)
(μg/mL)
(μL)
(Males)
Endpoints

1
0
0
50
2^b
Plasma TNFα,

2
0.25
5.0
50
2^b+ 2^c+ 2^g
IL-6, and

3
1.0
20
50
2^b+ 2^c+ 2^g
IFNα cytokine

4
4 × 1.0 μg
20
50
2^b+ 2^c+ 2^g
analysis

(4.0 μg total)

^b= plasma samples collected 6 hours post-dose,

^c= plasma samples collected 24 hours post-dose,

^g= plasma samples collected 8 days post-dose.

For TNFα analysis, plasma samples were analyzed using a commercially-available assay kit from R&D SYSTEMS (cat. no. RTA00). The rat TNFα ELISA is a solid phase enzyme-linked immunosorbent assay. The ELISA kit employs a TNFα-specific anti-rat monoclonal antibody for solid phase immobilization (pre-coated on the microtiter plate), and HRP conjugated to an anti-rat TNFα polyclonal antibody for detection. The reference standard provided with the kit is a recombinant rat TNFα. For each assay, plasma samples and standards were diluted with the diluent provided in each respective kit. For the TNFα ELISA, plasma samples were diluted 1:2. The standard curve consisted of six (6) serial 2-fold concentrations ranging from 800 to 12.5 pg/mL. Controls were reconstituted with diluent, and blanks contained diluent only.

Following sample, standard, control, or diluent addition (50 μL per well, each in duplicate), plates were incubated for 2 hours at room temperature. Following a wash step to remove unbound substances, HRP conjugate was added to each well (100 μL/well), and plates were incubated for 2 hours at room temperature. Following a second wash step, 100 μL/well TMB substrate was added to the plate. The plate was incubated for 30 minutes at room temperature, protected from light, to allow the color reaction to develop. The reaction was stopped following the addition of HCl Stop Solution (100 μL/well). The optical density was read on a SpectraMax 340 (MOLECULAR DEVICES) plate reader at 450 nm, within 30 minutes following addition of Stop Solution. The intensity of the color measured was in proportion to the amount of rat TNFα bound in the initial step. A standard curve was generated for each assay plate, and test sample TNFα concentrations were determined by interpolation of absorbance A450 values from the standard curve and dilution factor. The assay range for the TNFα kit is 12.5-800 μg/mL, with a minimum detectable concentration of less than 10 pg/mL (with the 1:2 minimum required dilution for plasma samples).

For IL-6 analysis, plasma IL-6 samples were analyzed using a commercially-available assay kit from R&D SYSTEMS (cat. no. R6000B—IL-6). The rat IL-6 ELISA is a solid phase enzyme-linked immunosorbent assay. The ELISA kit employs anti-rat IL-6 monoclonal antibody for solid phase immobilization (pre-coated on the microtiter plate), and HRP conjugated to IL-6-specific anti-rat polyclonal antibody for detection. The reference standard provided with the kit is a recombinant rat IL-6. Plasma samples were used undiluted (as provided), and standards were diluted with the diluent provided in the kit. The standard curve consisted of six (6) serial 2-fold concentrations ranging from 4,000 to 62.5 pg/mL. Controls were reconstituted with diluent, and blanks contained diluent only. Following sample, standard, control, or diluent addition (50 μL per well, each in duplicate), plates were incubated for 2 hours at room temperature. Following a wash step to remove unbound substances, HRP conjugate was added to each well (100 μL/well), and plates were incubated for 2 hours at room temperature. Following a second wash step, 100 μL/well TMB substrate was added to the plate. The plate was incubated for 30 minutes at room temperature, protected from light, to allow the color reaction to develop. The reaction was stopped following the addition of HCl Stop Solution (100 μL/well). The optical density was read on a SpectraMax 340 (MOLECULAR DEVICES) plate reader at 450 nm, within 30 minutes following addition of Stop Solution. The intensity of the color measured was in proportion to the amount of rat IL-6 bound in the initial step. A standard curve was generated for each assay plate, and test sample IL-6 concentrations were determined by interpolation of absorbance A450 values from the standard curve and dilution factor. The assay range for the IL-6 kit is 62.5-4,000 pg/mL, with a minimum detectable concentration of less than 21 pg/mL.

For IFNα analysis, plasma IFNα samples were analyzed using a commercially-available assay kit from NOVATEINBIO (cat. no. BG-RAT11380). The ELISA is a solid phase enzyme-linked immunosorbent assay. The ELISA kit employs anti-rat IFNα monoclonal antibody for solid phase immobilization (pre-coated on the microtiter plate), and HRP conjugated antibody specific for IFNα for detection. Plasma samples were diluted 1:4, and the standards were used as provided in the kit. Standards consisted of six (6) serial 2-fold concentrations ranging from 100 to 3.1 pg/mL. Blanks contained diluent only. Following sample, standard, or diluent addition (50 μL per well, each in duplicate), HRP conjugate was added to each well (100 μL/well), and plates were incubated for 1 hours at 37° C. Following a wash step, 50 μL/well each of Chromogen Solution A and Chromogen Solution B were added to the plate. The plate was incubated for 15 minutes at 37° C., protected from light, to allow the color reaction to develop. The reaction was stopped following the addition of Stop Solution (50 μL/well). The optical density was read on a SpectraMax 340 (MOLECULAR DEVICES) plate reader at 450 nm. The intensity of the color measured was in proportion to the amount of rat IFNα bound in the initial step. A standard curve was generated for each assay plate, and test IFNα concentrations were determined by interpolation of absorbance A450 values from the standard curve and dilution factor. The assay range for the IFNα kit is 3.1-100 pg/mL, with a minimum detectable concentration of less than 1 μg/mL (4 μg/mL, with the 1:4 dilution required for plasma samples).

The results of this study were, inter alia, that NOVECRIT was not detected at the injection site 24 h after dosing (as assayed by RT-PCR). More specifically, NOVECRIT was not detected in any of the following samples (RT-PCR): serum (6h, 24 h, 48h and 72h after dosing), liver (6h and 24 h after dosing), and kidney (6h and 24 h after dosing). Further, positive controls (spiked with NOVECRIT) yielded a robust signal in all tissues (as assayed RT-PCR). FIG. 18 depicts single administration of NOVECRIT induced a rapid increase and sustained level of NOVEPOIETIN in serum. The Y axis shows concentration of NOVEPOIETIN protein (mU/mL). This suggests, without wishing to be bound by theory, that the studied RNA therapeutic is able to provide therapeutically important pharmacodynamic properties. In fact, this PD behavior is vastly improved relative to wild type EPO (which is known in the art to have a half-life of about 4-12 hours).

Furthermore, serum spiked with NOVECRIT showed near-instant degradation of the RNA (as assayed by RT-PCR). Without wishing to be bound by theory, this data indicates that the present RNA therapeutics are safe and have little chance of toxicity limitations, for example, liver or kidney toxicities.

With respect to cytokines, IL-6 and TNFα cytokine levels were below assay detection thresholds for all animals at each of the timepoints evaluated in this study. Low levels of IFN-α were only detectable in the Day 8 samples from animals in Groups 3 and 4. FIG. 20 depicts a table summarizing TNFα, IL-6, and IFNα cytokine levels in plasma samples collected from a maximum tolerated dose of NOVECRIT in male Sprague Dawley rats. Without wishing to be bound by theory, this data indicates that the present RNA therapeutics do not stimulate an unfavorable immunogenicity which has limited the therapeutic utility of certain RNA therapeutics.

Example 36: Gene Editing of the COL7A1 Gene

The present RNA-based gene editing approaches were applied to the COL7A1 gene. This gene is of interest because, inter alia, it is frequently involved in dystrophic epidermolysis bullosa. FIG. 21 depicts a SURVEYOR assay using the DNA of primary adult human dermal fibroblasts transfected with RNA TALENs targeting the sequence TGAGCAGAAGTGGCTCAGTG (SEQ ID NO: 467) and TGGCTGTACAGCTACACCCC (SEQ ID NO: 468), located within the COL7A1 gene. The bands present in the +RNA lane indicate editing of a region of the gene that is frequently involved in dystrophic epidermolysis bullosa. FIG. 22 depicts another SURVEYOR assay using the DNA of primary adult human dermal fibroblasts transfected with RNA TALENs, now targeting the sequence TTCCACTCCTGCAGGGCCCC (SEQ ID NO: 469) and TCGCCCTTCAGCCCGCGTTC (SEQ ID NO: 470), located within the COL7A1 gene. The bands present in the +RNA lane indicate editing of a region of the gene that is frequently involved in dystrophic epidermolysis bullosa. This data points to, among others, a gene editing approach to the treatment of certain genetic disorders such as dystrophic epidermolysis bullosa.

Example 37: Gene-Editing of the MYC Gene Using a Synthetic RNA with Non-Canonical Nucleotides

Experiments were conducted with in vitro transcribed synthetic RNA molecules containing non-canonical nucleotides and encoding gene-editing proteins. The immunogenicity and the gene-editing efficiency of in vitro transcribed synthetic RNA molecules having (1) only pseudouridine (psU) as a non-canonical nucleotide; (2) only 5-methylcytidine (5mC) as a non-canonical nucleotide; and (3) both of pseudouridine and 5-methylcytidine as non-canonical nucleotides was evaluated (as well as controls).

Specifically, RNA containing the following nucleotide combinations: (i) A,G,U,C, (ii) A,G,psU,C, (iii) A,G,U,5mC, and (iv) A,G,psU,5mC, and encoding TALEN pairs targeting the following DNA sequences, which can be found within the MYC gene: TCGGCCGCCGCCAAGCTCGT (SEQ ID NO: 474) and TGCGCGCAGCCTGGTAGGAG (SEQ ID NO: 475), were synthesized according to the methods described herein. Human dermal fibroblasts (MA001SK) were plated in 6-well and 24-well tissue culture plates in DMEM with 10% FBS at 100,000 and 10,000 cells per well, respectively. The next day, the cells were transfected in the 6-well plate with 2 μg of RNA (1 μg for each component of the TALEN pair) and the cells were transfected in the 24-well plate with 0.2 μg of RNA (0.1 μg for each component of the TALEN pair) according to the methods described herein. 24 hours after transfection, the total RNA from the cells in the 24-well culture plate was isolated using an RNeasy Mini Kit (74106; QIAGEN), including isolating the total RNA from a sample of cells that had not been transfected with RNA (negative control; “Neg.” in FIG. 23). The genomic DNA was removed by a 15 minute digestion with DNase I (RNase-Free) (M0303L; NEW ENGLAND BIOLABS) and the reaction purified using an RNeasy Mini Kit. 1 μL of total RNA was used to assess gene expression by real-time RT-PCR using TAQMAN gene-expression assays (APPLIED BIOSYSTEMS) designed to detect expression of the immunogenicity markers TLR3, IFIT1, and IFIT2 (FIG. 23). The data were normalized to both the positive experimental control sample (“A,G,U,C”) and to a loading control (GAPDH).

48 hours after transfection, the genomic DNA was isolated from the cells in the 6-well culture plate using a DNeasy Blood and Tissue Kit (69506; QIAGEN), including from a sample of cells that had not been transfected with RNA (negative control, “Neg.” in FIG. 24). A 970 bp region of the MYC gene surrounding the predicted TALEN cut location was amplified using a 35 cycle 2-step PCR reaction containing the following primers: TAACTCAAGACTGCCTCCCGCTTT (SEQ ID NO: 476) and AGCCCAAGGTTTCAGAGGTGATGA (SEQ ID NO: 477). 160 ng was hybridized in 5 μL of amplified sequence from RNA-treated cells to 160 ng in 5 μL of amplified sequences from untreated MA001SK cells by mixing the two sequences with 0.5 μL of 1M KCl and 0.5 μL of 25 mM MgCl₂and running the following program in a thermocycler: 95° C. for 10 minutes; 95° C. to 85° C. at 0.6250/s; 85° C. to 25° C. at 0.125 C/s. The SURVEYOR assay was performed by adding 0.5 μL of SURVEYOR nuclease and 0.5 μL of Enhancer from the SURVEYOR Mutation Detection Kit (7060201; INTEGRATED DNA TECHNOLOGIES) to the hybridized product, mixing, and incubating at 42° C. for 25 minutes. The protocol above was also used to process the positive control DNA sample provided with the SURVEYOR Mutation Detection Kit as a positive experimental control for the SURVEYOR Assay (“Assay Pos.” in FIG. 24). Samples were analyzed by agarose gel electrophoresis (FIG. 24). For each sample, gene-editing efficiency was calculated as a ratio of the intensity of the digested bands (indicated by “*” in FIG. 24) to that of the undigested band.

As shown in FIG. 23 below, the samples from cells transfected with the positive control RNA (A,G,U,C), and the samples from cells transfected with RNA containing either pseudouridine or 5-methylcytidine exhibited upregulation of all three of the immunogenicity markers TLR3, IFIT1, and IFIT2. The sample from cells transfected with RNA containing both pseudouridine and 5-methylcytidine exhibited negligible upregulation of the immunogenicity markers (less than 0.01-fold of the positive control), demonstrating that in vitro transcribed synthetic RNA with both pseudouridine and 5-methylcytidine and encoding a gene-editing protein can evade detection by the innate-immune system of mammalian cells.

Further, as shown in FIG. 24 below, the sample from cells transfected with RNA containing both pseudouridine and 5-methylcytidine exhibited highly efficient gene editing (41.7%), which was greater than the efficiency exhibited by samples from cells transfected with RNA containing pseudouridine alone (35.2%), demonstrating that in vitro transcribed synthetic RNA comprising both pseudouridine and 5-methylcytidine and encoding a gene-editing protein can both (i) gene-edit mammalian cells at high efficiency, and (ii) gene-edit mammalian cells at higher efficiency than in vitro transcribed synthetic RNA comprising pseudouridine and not comprising 5-methylcytidine.

Example 38: COL7A1 Gene Editing and Repair in Human Cells

RNA Synthesis

Reaction

Template
Nucleotides
Volume/μL
ivT Yield/μg

COL7A1 TALEN 1L
A, G, 5moU, C
20
120.528

COL7A1 TALEN 1R
A, G, 5moU, C
20
120.204

COL7A1 TALEN 1L
A, G, 5moU, C
15
81.94

COL7A1 TALEN 1R
A, G, 5moU, C
15
61.88

50,000 primary human epidermal keratinocytes (HEKn, Gibco) were plated in wells of 6-well plates in EpiLife+Supplement S7. The next day, cells were transfected according to Example 3 with 1 μg of RNA encoding each component of the gene editing pair and 2 μg of a single-stranded DNA repair template having a length of 60, 70, 80, 90 or 100 nucleotides (“nt”). 48 hours after transfection, genomic DNA was purified. A segment of the COL7A1 gene was amplified using the primers GCATCTGCCCTGCGGGAGATC (SEQ ID NO: 478) and CCACGTTCTCCTTTCTCTCCCCGTTC (SEQ ID NO: 479), which produce a 535 bp amplicon. The efficiency of gene editing was assessed using T7 Endonuclease I (“T7E1”, New England Biolabs) according to the manufacturer's instructions. Bands of approximately 385 bp and 150 bp indicate successful gene editing. FIG. 25 and FIG. 29 show the result of digestion with T7EI, analyzed by agarose gel electrophoresis. FIG. 27 and FIG. 29 show the result of digestion with MluI-HF, analyzed by agarose gel electrophoresis. Because the repair template contains the sequence ACGCGT (SEQ ID NO: 480), digestion of the amplified product with MluI-HF (New England Biolabs) produces bands of approximately 385 bp and 150 bp in the case of successful gene repair.

RNA encoding gene editing proteins targeting the following sequences in the COL7A1 gene was synthesized according to Example 1: TGAGCAGAAGTGGCTCAGTG (SEQ ID NO: 473) and TGGCTGTACAGCTACACCCC (SEQ ID NO: 468). 50,000 primary human epidermal keratinocytes (HEKn, Gibco) were plated in wells of 6-well plates in EpiLife+Supplement S7. The next day, cells were transfected according to Example 3 with 1 μg of RNA encoding each component of the gene editing pair and 1-4 μg of an 80 nucleotide single-stranded DNA repair template. 48 hours after transfection, genomic DNA was purified. A segment of the COL7A1 gene was amplified using the primers GCATCTGCCCTGCGGGAGATC (SEQ ID NO: 481) and CCACGTTCTCCTTTCTCTCCCCGTTC (SEQ ID NO: 482), which produce a 535 bp amplicon. The efficiency of gene editing was assessed using T7 Endonuclease I (“T7E1”, New England Biolabs) according to the manufacturer's instructions. Bands of approximately 385 bp and 150 bp indicate successful gene editing. FIG. 26 show the result of digestion with T7E1, analyzed by agarose gel electrophoresis. FIG. 28 show the result of digestion with MluI-HF, analyzed by agarose gel electrophoresis. Because the repair template contains the sequence ACGCGT (SEQ ID NO: 480), digestion of the amplified product with MluI-HF (New England Biolabs) produces bands of approximately 385 bp and 150 bp in the case of successful gene repair.

Example 39: Expression of BMP7 Variants in Human Cells

RNA encoding wild type BMP7 and RNA encoding variants of BMP7 was synthesized according to Example 1 (see also table below).

RNA Synthesis

Reaction

Template
Nucleotides
Volume/μL
ivT Yield/μg

BMP7 Wild Type
A, G, 5moU, C
15
125.8

BMP7 Variant A
A, G, 5moU, C
15
120.36

BMP7 Variant B
A, G, 5moU, C
15
143.14

BMP7 Variant C
A, G, 5moU, C
15
106.42

50,000 primary human dermal fibroblasts (MA001SK, Factor Bioscience) or 100,000 primary human epidermal keratinocytes (HEKn, Gibco) were plated in DMEM+10% FBS or EpiLife+Supplement S7, respectively. Cells were transfected according to Example 3 with 1 μg of RNA encoding wild type BMP7 or a variant thereof. 24 hours after transfection, the medium was sampled and secreted BMP7 levels were measured with a human BMP7 ELISA kit (ab99985, Abcam) using medium diluted 10-fold according to the manufacturer's instructions. Secreted BMP7 levels were determined by measuring 450 nm absorbance using a microplate reader (EMax Plus, Molecular Devices). Secreted BMP7 levels are shown in FIG. 30.

Example 40: Expression of Parathyroid Hormone (PTH) in Human Cells

RNA encoding PTH was synthesized according to Example 1 (see also table below).

RNA Synthesis

Reaction

Template
Nucleotides
Volume/μL
ivT Yield/μg

PTH
A, G, 5moU, C
15
51.68

100,000 human epidermal keratinocytes (HEKn, Gibco) were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 1 μg of RNA encoding PTH. 24 hours after transfection, the medium was sampled and secreted PTH levels were measured using a human PTH ELISA kit (EIA-PTH-1, Ray Biotech) according the manufacturer's instructions. Secreted PTH levels were determined by measuring 450 nm absorbance using a microplate reader (EMax Plus, Molecular Devices). Secreted PTH levels are shown in FIG. 31.

Example 41: Intradermal, Subcutaneous, Rectal and Nasal Administration of NOVECRIT for the Treatment of Anemia

A repeat dose toxicity study of Novecrit was conducted for the treatment of anemia. Specifically, 8-10 weeks old male Sprague Dawley rats were administered with Novecrit via intradermal, subcutaneous, rectal, or nasal routes once per day on days 1, 8, and 15. The animals were assigned to groups and treated as indicated in the table below:

Dose
Dose
Dose
Number of

Group
Test
Dose
Level
Concentration
Volume
Animals

Group
Color
Article
Route
(μg)
(μg/mL)
(μL)
Males

1
White
Control
Intradermal
0.0
0.0
50
3^a+ 8^b

2
Yellow
Novecrit
Intradermal
4X (0.25 μg/
5.0
50
3^a+ 8^b

injection;

1.0 μg total)

3
Green
Novecrit
Subcutaneous
4.0
20.0
200
3^a+ 8^b

4
Blue
Novecrit
Rectal
4.0
20.0
200
3^a+ 8^b

5
Red
Novecrit
Nasal
4.0
20.0
200
3^a+ 8^b

Note:

Total dose volume (μL/per animal) are constant

^aToxicity animals (also called main study animals), necropsy on Day 44

^bTK animals, euthanized as n = 2/time point on Days 2, 16, 23 and 44

More particularly, Group 1 was dosed via intradermal injection. Each dose was administered in four intradermal injections of 50 μL/injection for a total of 200 uL per animal. Injections were carried out on previously marked sites near the midline of the dorsal lumbar area (upper left, upper right, lower left and lower right quadrants). Group 2 was dosed four intradermal injections at 0.25 μg each (1.0 μg total) into previously marked sites near the midline of the dorsal lumbar area (upper left, upper right, lower left and lower right quadrants). Group 3 was dosed by subcutaneous injections into an area of the back located in the lower dorsal thoracic/lumbar region. Group 4 was dosed by rectal administration. The animal was manually restrained and the abdomen of each rat was manually palpated to remove any fecal matter. If deemed necessary, the rectum was lavaged with up to 2 ml of saline for enema followed by manual palpation of the abdomen to remove the fecal matter, if any. Novecrit was drawn into a syringe and an appropriately sized gavage needle with a rounded tip (ball) was attached to the syringe. A lubricant jelly was applied to the insertion device to aid with insertion; it was advanced approximately 1 cm into the lumen of the colon and the Novecrit instilled. The rat was then maintained in a head-down position for approximately 20-30 seconds to limit the expulsion of solution. Group 5 was dosed via nasal route. The animal was anesthetized (per SNBL USA SOP). The animal was laid on its back with the head elevated. The dose was dispensed slowly into the nares. Approximately half the dose volume was administered to one nare. The remaining dose volume was administered to another nare. The first dose was administered on Day 1, and the last dose on Day 15.

The rats were clinically monitored including their food consumption and body weight. In addition, blood was collected for hematology, coagulation, and serum chemistry analysis as indicated below:

Toxicity Group Specimen Collection Frequency

Time Point
Hematology
Coagulation
Serum Chemistry

Day 44
1X
1X
1X

(n = 3/Group)
(n = 3/Group
(n = 3/Group)

X= Number of times procedure performed

Analysis was done on the toxicokinetic group as follows:

In-Text Table 3: Toxicokinetic Group Specimen Collection Frequency

Time Point
Hematology^a
TK
Cytokines

Acclimation
1X
—
—

(n = 8/Group)

Day 2
—
1X
1X

(n = 2/Group,
(n = 2/Group,

24 hours postdose)
24 hours postdose)

Day 8
Pre-dose
—
—

(n = 6/Group)

Day 15
Pre-dose
—
—

(n = 6/Group)

Day 16
—
1X
1X

(n = 2/Group,
(n = 2/Group,

24 hours postdose)
24 hours postdose)

Day 22
1X
—
—

(n = 4/Group)

Day 23
—
1X
1X

(n = 2/Group)
(n = 2/Group)

Day 29
1X
—
—

(n = 2/Group)

Day 36
1X
—
—

(n = 2/Group)

Day 43
1X
—
—

(n = 2/Group)

Day 44
—
1X
1X

(n = 2/Group)
(n = 2/Group)

X= Number of times procedure performed

TK = toxicokinetics

Terminal necropsy for toxicity animals occurred on Day 44. TK animals were euthanized on Days 2, 16, 23, and 44. Pathological analysis was conducted on the animals.

As shown in FIG. 32, administration of NOVECRIT stimulated erythropoiesis resulting in elevated hematocrit for at least 14 days in all four study groups compared to control.

Example 42: Intradermal Administration of RNA Encoding BMP7 Variants for the Treatment of Diabetic Nephropathy

A ZDSD rat model was utilized to study the effects of RNAs encoding BMP7 variants for the prevention and treatment of diabetic nephropathy. Specifically, ZDSD rats were treated with RNAs encoding BMP7 variants administered intradermally. A schematic of the study design is provided in FIG. 33. The animals were assigned to groups and treated as indicated in the table below:

Dose
Dose
Dose

Group

No. of
Level
Conc.
Volume

Route of

Number
Treatment
Animals
(μg)
(μg/ml)
(ml/kg)
Frequency
Admin.

1
Vehicle
24
0
0
0.1
2x/wk
I.D.

2
FTB-F1 (wt
6
1
5
0.1
2x/wk
I.D.

BMP7)

3
FTB-F2 (BMP7
6
1
5
0.1
2x/wk
I.D.

variant A)

4
FTB-F3 (BMP7
6
1
5
0.1
2x/wk
I.D.

variant B)

5
Lisinopril
6
Standard
Standard
5
Continuous
Diet

6^a
Vehicle
20
0
0
0.2/rat
3x/wk
I.D.

7^a
FTB-F2 (BMP7
10
2
10
0.2/rat
3x/wk
I.D.

variant A)

^aGroup 1 animals at the end of the “Prevention” arm were randomized into Groups 6 and 7 (n = 20 or 10 each, respectively) to define the “Treatment” arm. The remaining 6 animals from Group 1 were euthanized for kidney collection and blood draw at the end of the “Prevention” arm.

To study the effect of the RNAs on prevention of diabetic nephropathy, animals were delivered from the barrier (PCO-5638) to the vivarium (PCO-Rm C) and allowed 3 days for acclimation. All animals were placed on 5SCA diabetogenic diet for 3 weeks (weekly body weight measurements were taken). Animals were then returned to regular 5008 diet for the duration of the study (weekly body weight measurements were taken). After 2 weeks on 5008 diet, body weight measurement, blood draw (500 ul) and a 24 hour baseline urine collection (urinary albumin and creatinine measurements) were performed on all animals. 36 rats were randomized to groups 1, 2 and 3 based on body weight, glucose and urinary albumin. Groups 1, 2, 3 and 4 received either vehicle or test article (i.d.) via intradermal administration (2×/week, Mon and Thur). Group 5 was administered Lisinopril admixed in the 5008 diet (food consumption will begin on this group of animals). Blood draws (500 ul, tail vein) were taken from Groups 2, 3 and 4, 24 hours after each dose for the first 2 weeks and then 1×/week thereafter. After 4 weeks of dosing, blood sample via tail vein (500 ul) was taken from all animals (Groups 1-5). At the end of 8 weeks of dosing, a 24 hour urine collection was performed (urinary albumin and creatinine measurements) together with a tail vein (500 ul) blood sample from Group 1 (n=18 going into the Treatment arm). Results for urine volume, creatinine, and albumin is shown in FIG. 35. As shown in FIG. 35, treatment with RNA encoding BMP7 Variant A resulted in reduced levels of urine albumin in rats afflicted with diabetic nephropathy compared to the control, indicating superior kidney function in the treated animals compared to the control animals (placebo group). To terminate the study, all animals were euthanized with CO₂asphyxiation and a blood draw was performed via cardiac puncture to collect serum for BUN, creatinine and glucose, and plasma for compound exposure and biomarker analysis. Both kidneys were dissected and fixed for histology. FIG. 34 shows the serum level of BMP7 protein in Groups 1, 2, 3, and 4. Results indicate that the serum levels of BMP7 protein in these groups were as follows: Group 4, Group 3>Group 2, Group 1.

A study was also conducted to analyze the effect of the RNAs encoding BMP7 variants on treating diabetic nephropathy. Specifically, the vehicle animals from Group 1 (Prevention arm, n=18) were randomized based on body weight, glucose and urine albumin (Groups 6, n=20; 7, n=10;). Animals received either Vehicle or BMP7 variant-encoding RNAs (intradermal, 2×/week, Mon and Thur), groups 6 and 7, respectively. After 4 weeks of treatment, animals were euthanized with CO₂asphyxiation and a blood draw will be performed via cardiac puncture to collect serum for BUN, creatinine and glucose, and plasma for compound exposure and biomarker analysis. Both kidneys were dissected and fixed for histology. As shown in FIG. 36, treatment with RNA encoding BMP7 Variant A significantly decreased urine albumin level in rats afflicted with diabetic nephropathy (p=0.0015), indicating superior kidney function in the treated animals. In addition, the treatment group showed significantly lower kidney weight at termination (p=0.018), and significantly lower serum creatinine at termination (p=0.016). These results suggested that BMP7 variant-encoding RNA effectively treated diabetic nephropathy and promoted superior kidney function compared to the control animals (placebo group).

An illustrative study protocol is provided below:

Week
Day
Procedure

15 weeks
−7
Animals arrive in Room C from the barrier

old

0
BW*, Stat Strip, and Place animals on 5SCA for 3

wks

1
7
BW*, Stat Strip

2
14
BW*, Stat Strip

3
21
BW*, Stat Strip, put animals on 5008

4
28
BW*, Stat Strip

34
BW*, Tail vein blood draw and randomize for

Prevention arm (Grps 1, 2, 3, 4, 5)

5
35
Begin 24 hr urine collection

36
Collect 24 hr urine

39
BW*

Intradermal dosing Grps 1, 2, 3 and 4, and put Grp

5 on admixed 5008 diet

Begin food consumption on group 5

40
Collect 24 blood sample for exposure (gps 2, 3, & 4)

42
Dosing

43
Collect 24 blood sample for exposure (gps 2, 3, & 4)

6 (1 P)
46
BW*, Dosing

Food consumption group 5

47
Collect 24 blood sample for exposure (gps 2, 3, & 4)

49
Dosing

50
Collect 24 blood sample for exposure (gps 2, 3, & 4)

7 (2 P)
53
BW*, Dosing

Food consumption group 5

54
Collect 24 blood sample for exposure (gps 2, 3, & 4)

56
Dosing

8 (3 P)
60
BW*, Dosing

Food consumption group 5

61
Collect 24 blood sample for exposure (gps 2, 3, & 4)

63
Dosing

9 (4 P)
67
BW*, Dosing

Food consumption group 5

Blood draw (tail vein)

68
Blood draw (all groups)

Collect 24 blood sample for exposure (gps 2, 3, & 4)

70
Dosing

10 (5 P)
74
BW*, Dosing

Food consumption group 5

75
Collect 24 blood sample for exposure (gps 2, 3, & 4)

77
Dosing

11 (6 P)
81
BW*, Dosing

Food consumption group 5

Collect 24 blood sample for exposure (gps 2, 3, & 4)

84
Dosing

12 (7 P)
88
BW*, Dosing

Food consumption group 5

89
Collect 24 blood sample for exposure (gps 2, 3, & 4)

91
Dosing

13 (8 P)
95
BW*, dosing

Food consumption group 5

Blood draw (tail vein) (all groups)

96
Begin 24 hr urine collection

97
Collect 24 hr urine

Terminate Groups 1 (n = 6), 2, 3, 4 and 5 cardiac

puncture blood draw, and collect kidneys, weigh and

fix.

98
BW* and randomize (BW*, glucose, albumin) Group

1 (n = 18) rats into Groups 6, 7 and 8

Dose

Food Consumption group 8

103
Dose

14 (1 T)
106
BW*, Dosing

Food Consumption group 8

110
Dosing

15 (2 T)
113
BW*, Dosing

Food Consumption group 8

117
Dosing

16 (3 T)
120
BW*, Dosing

Food Consumption group 8

124
Dosing

17 (4 T)
127
BW*, Terminate, cardiac puncture blood draw, and

collect kidneys, weigh and fix.

*BW: body weight measurement

In an illustrative study, the protocol was amended from above towards the end of the study as follows:

Week

BW

[Treatment Arm]
Day
Begin 24 hr urine collection

81
Collect 24 hr urine

BW, Blood draw

Randomize (BW, glucose, albumin)

rats into Groups 6 and 7

1 T

BW, Dose

12 hr PK sample

Dose

Dose and Begin 24 hr Urine collection

89
Collect 24 hr Urine and Dose

2 T

BW, Dose

12 hr PK sample

Dose

Begin 24 hr Urine collection

96
Collect 24 hr Urine and Dose

3 T

BW, Dose

12 hr PK sample

Dose

Begin 24 hr Urine collection

Collect 24 hr Urine and Dose (last dose)

4 T

BW

No activity

Begin 24 hr urine collection

Collect 24 hr urine

BW, Terminate, cardiac puncture

blood draw, and collect kidneys,

weigh and fix.

During both studies, pathological analysis is conducted as follows:

Specimen to
Method of
Anti-
Specimen to
Clinical
Method of

be Collected
Collection
Coagulant
be Analyzed
Chemistry
Analysis

Urine
24 hr
NA
Urine
Creatinine/
AU480/

Metabolism

Albumin
ICL Elisa

Cages

Whole Blood
Tail Vein
None
Serum
BUN
AU480/

Creatinine
Sponsor

Glucose/

Whole Blood
Cardiac
None
Serum
BUN
AU480/

Puncture

Creatinine
Sponsor

(Grps 1, 2, 3,

Glucose/

4 and 5; and

Cmpd Exp &

6, 7, 8)

Biomarker

Altogether, these results suggested that RNAs encoding BMP-7 variants effectively prevented the development of diabetic nephropathy in rats. In addition, these RNAs also effectively treated and restored kidney functions in rats already afflicted with diabetic nephropathy. RNA encoding BMP7 Variant A was particularly effective at preventing and treating diabetic nephropathy in rats.

Example 43: Transfection of Human Keratinocytes with RNA Encoding BDNF, BMP-2, BMP-6, IL-2, IL-6, IL-15, IL-22, LIF or FGF-21

100,000 human epidermal keratinocytes (HEK, Gibco) were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg of RNA encoding BDNF, BMP-2, BMP-6, IL-2, IL-6, IL-15, IL-22, LIF or FGF-21. 24 hours after transfection, the medium was sampled and secreted protein levels were measured using a human ELISA kit (see Table below) according the manufacturer's instructions. Secreted protein levels were determined by measuring 450 nm absorbance using a microplate reader (EMax Plus, Molecular Devices). Secreted protein levels are shown in FIG. 34, panels A-I.

Protein
Part Number
Vendor

BDNF
DBNT00
R&D Systems

BMP-2
DBP200
R&D Systems

BMP-6
ab99984
Abcam

IL-2
D2050
R&D Systems

IL-6
D6050
R&D Systems

IL-15
D1500
R&D Systems

IL-22
D2200
R&D Systems

LIF
DLF00
R&D Systems

FGF-21
DF2100
R&D Systems

Example 44: Transfection of Human Keratinocytes with RNA Encoding IL-15 and/or IL-15RA

100,000 human epidermal keratinocytes (HEK, Gibco) were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg of RNA encoding IL-15 or 1 μg each of RNA encoding IL-15 and RNA encoding IL-15RA. 24 hours after transfection, the medium was sampled and secreted IL-15 levels were measured using a human IL-15 ELISA kit (D1500, R&D Systems) according the manufacturer's instructions. Secreted IL-15 levels were determined by measuring 450 nm absorbance using a microplate reader (EMax Plus, Molecular Devices). Secreted IL-15 levels are shown in FIG. 35. As demonstrated in FIG. 35, co-transfection of IL-15 and IL-15RA significantly increased secreted IL-15 levels compared to transfection with IL-15 alone.

Example 45: Pharmacokinetic Study Via Intradermal Injection in Rats

Studies were conducted to evaluate the responses of Sprague Dawley rats to intradermal administration of various RNAs. Specifically, 8-10 weeks old, female Sprague Dawley rats weighing about 200 g to about 350 g were used for this study. A total of 33 rats were tested, and the animals were assigned to study groups and treated as indicated in the Table below:

Dose
Dose
Dose
Number of

Group
Test
Dose
Level
Concentration
Volume
Animals

Group
Color
Article
Route
(μg)
(μg/mL)
(μL/per injection)^a
Females

1
White
Control
ID
4.0
20.0
200
3^b

(NOVEPOEITIN)

(4 × 50)

2
Yellow
TA1
ID
4.0
20.0
200
3^b

(IL2)

(4 × 50)

3
Green
TA2
ID
4.0
20.0
200
3^b

(IL6)

(4 × 50)

4
Blue
TA3
ID
4.0
10.0
200
3^b

(IL15)

(4 × 50)

5
Red
TA4
ID
4.0
20.0
200
3^b

(IL15 + IL15RA)

(10.0 each)
(4 × 50)

6
Dark Grey
TA5
ID
4.0
20.0
200
3^b

(IL22)

(4 × 50)

7
Purple
TA6
ID
4.0
20.0
200
3^b

(BMP2)

(4 × 50)

8
Black
TA7
ID
4.0
20.0
200
3^b

(BDNF)

(4 × 50)

9
White/Yellow
TA8
ID
4.0
20.0
200
3^b

(LIF)

(4 × 50)

10
Green/Blue
TA9
ID
4.0
20.0
200
3^b

(PTH)

(4 × 50)

11
Red/Dark Grey
TA10
ID
4.0
20.0
200
3^b

(FGF21)

(4 × 50)

^aTotal dose volume (μL/per animal) was constant. Each animal received four intradermal injections of 50 μL/per injection for a total of 200 μL per animal.

^bAnimals, euthanized on Day 3 without examination or necropsy

Intradermal = ID

For the study, the animals were treated with 4 ug of RNA. All groups were dosed via intradermal injection. Each dose was administered in four intradermal injections of 50 μL/injection for a total of 200 uL per animal. Injections occurred into previously marked sites near the midline of the dorsal lumbar area (upper left, upper right, lower left and lower right quadrants). Dose time (after the last injection) was recorded. Additional markings were made as needed to allow for identification of the dose site. Animals were administered with the RNAs on day 1 and euthanized on day 3. Clinical observations were made on the rats twice daily. Food consumption and body weight were also monitored.

During the study, approximately 1 ml of blood samples was collected from the jugular vein for pharmacokinetic analysis as follows:

Time Point
PK^a

Acclimation
—

Day 1
12 hours postdose

Day 2
24 hours postdose

Day 3
48 hours postdose

^aTime points for blood collection (n = 3 animals/Group/Time point)

PK = Pharmacokinetics

Results indicate that following administration of RNAs encoding FGF21, IL15, IL15 and IL15R, IL6, IL22, and NOVEPOEITIN, these proteins were readily detected in the blood with protein levels peaking at approximately 12 hours post injection (FIG. 39). Of note, the proteins tested in this study can be taken up by cells and tissues and/or can exert an effect near the site of expression without appreciable accumulation in systemic circulation.

Example 46: Wound-Healing Therapy Comprising RNA Encoding IL22

Primary human epidermal keratinocytes (HEKn-APF) were plated in 6-well plates coated with recombinant human Type-1 collagen at a density of 200,000 cells/well in keratinocytes medium (EpiLife+Supplement S7). After 24h, cells were transfected with 2 μg/well RNA encoding IL22 synthesized according to Example 1 and 50 mM D-Glucose was added to the wells. The following day, a line was scratched in each well using a plastic 10 μL pipette tip. Wells were imaged at 0h and 24 h after scratching. The size of the scratch was measured using at both times, and healing was determined by calculating the ration of the size of the scratch at 24h to the size of the scratch at 0h (FIG. 40).

Example 47: A1AT Gene Editing and Repair in Human Cells

RNA encoding gene-editing proteins targeting the following sequences in the A1AT gene was synthesized according to Example 1: L1: TAAGGCTGTGCTGACCATCG (SEQ ID NO: 611), R1: TAAAAACATGGCCCCAGCAG (SEQ ID NO: 612), and R2: TCTAAAAACATGGCCCCAGC (SEQ ID NO: 613). Cells were gene edited and gene-editing efficiency was measured according to Example 38 (FIG. 41). Additionally, cells were co-transfected with gene editing proteins targeting sequences L1 and R1 and a repair template comprising the sequence: cccctccaggccgtgcataaggctgtgctgaccatcgacgtcaaagggactgaagctgctggggccatgtttttagaggcc (SEQ ID NO: 614), and gene-repair efficiency was measured according to Example 38, using the AatII enzyme (FIG. 42). Another sample of cells was transfected with gene-editing proteins targeting the following sequences in the A1AT gene: L2: TGCCTGGTCCCTGTCTCCCT (SEQ ID NO: 615) and R3: TGTCTTCTGGGCAGCATCTC (SEQ ID NO: 616). Gene-editing efficiency was measured according to Example 38 (FIG. 43).

Example 48: Alpha-1-Antitrypsin Deficiency Therapy Comprising RNA Encoding Gene-Editing Proteins and a Repair Template

RNA encoding gene-editing proteins targeting the following sequences: L: TAAGGCTGTGCTGACCATCG (SEQ ID NO: 611) and R: TAAAAACATGGCCCCAGCAG (SEQ ID NO: 612) and a repair template comprising the sequence: RT: cccctccaggccgtgcataaggctgtgctgaccatcgacgagaaagggactgaagctgctggggccatgtttttagaggcc (SEQ ID NO: 617) are formulated with a vehicle according to the methods of the present invention and delivered to a subject afflicted with A1AT deficiency by intraportal administration, resulting in correction of the Z mutation in the subject's liver cells, reduction of polymerized Z protein accumulation in the subject's liver cells, increased secretion and serum levels of functional A1AT, and amelioration of one or more of the subject's symptoms.

Example 49: Friedrich's Ataxia Therapy Comprising RNA Encoding Gene-Editing Proteins

RNA encoding one or more gene-editing proteins capable of creating one or more double strand breaks in FXNA (SEQ ID NO: 582) and RNA encoding one or more gene-editing proteins capable of creating one or more double strand breaks in FXNB (SEQ ID NO: 583) are synthesized according to Example 1. RNA is formulated according to the methods of the present invention, and delivered to the heart of a patient with Friedrich's ataxia using a catheter. Target-sequence pairs are selected from: Pair 1: TCCCACACGTGTTATTTGGC (SEQ ID NO: 618) and TGGCAACCAATCCCAAAGTT (SEQ ID NO: 619); Pair 2: TAATAAATAAAAATAAAAAA (SEQ ID NO: 620) and TTGCCTATTTTTCCAGAGAT (SEQ ID NO: 621).

Example 50: Alpha-1-Antitrypsin Deficiency Therapy Comprising RNA Encoding Gene-Editing Proteins

RNA encoding one or more gene-editing proteins capable of creating one or more double strand breaks in A1AT_A (SEQ ID NO: 584) is synthesized according to Example 1. RNA is formulated according to the methods of the present invention, and is delivered to the liver of a patient with A1AT deficiency by intraportal injection.

Example 51: Alpha-1-Antitrypsin Deficiency Therapy Comprising RNA Encoding Gene-Editing Proteins and a Short Repair Template

RNA encoding one or more gene-editing proteins capable of creating one or more double strand breaks in A1AT_B (SEQ ID NO: 585) is synthesized according to Example 1. RNA and a single stranded DNA sequence containing the sequence: A1AT_RT (SEQ ID NO: 586) are formulated according to the methods of the present invention, and are delivered to the liver of a patient with A1AT deficiency by intraportal injection.

Example 52: Gene-Editing Proteins Comprising DNA-Binding and Deaminase Activity

RNA encoding a gene-editing protein comprising two or more repeat sequences, followed by: BASE_EDIT_FRONT (SEQ ID NO: 587), followed by any of: BASE_EDIT_ADA1 (SEQ ID NO: 588), BASE_EDIT_ADA2 (SEQ ID NO: 589), BASE_EDIT_ADA3 (SEQ ID NO: 590), BASE_EDIT_ADA4 (SEQ ID NO: 591), BASE_EDIT_CDA1 (SEQ ID NO: 592) and BASE_EDIT_CDA2 (SEQ ID NO: 593) is synthesized according to Example 1. The gene-editing protein is capable of correcting one or more mutations within 1 to 50 bases downstream of the target sequence.

Example 53: Gene Editing of A1AT, COL7A1, HBB, and PD-1

With reference to Example 1, Table 4, the following templates and targets were used:

SEQ

Target
ID

Template
Sequence
NO:

A1AT TALEN L
TGCCTGGTCC
615

CTGTCTCCCT

A1AT TALEN R
TGTCTTCTGG
616

GCAGCATCTC

A1AT RIBOSLICE LA
TGCCTGGTCC
615

CTGTCTCCCT

A1AT RIBOSLICE LB
TGCCTGGTCC
615

CTGTCTCCCT

A1AT RIBOSLICE R_A
TGTCTTCTGG
616

GCAGCATCTC

A1AT RIBOSLICE R_B
TGTCTTCTGG
616

GCAGCATCTC

COL7A1 exon 73 TALEN L
TATTCCCGGG
622

CTCCCAGGCA

COL7A1 exon 73 TALEN R
TCTCCTGGCC
612

TTCCTGCCTC

COL7A1 exon 73 rs3L 50A
TATTCCCGGG
622

CTCCCAGGCA

COL7A1 exon 73 rs3L 50B
TATTCCCGGG
622

CTCCCAGGCA

COL7A1 exon 73 rs3R 50A
TCTCCTGGCC
612

TTCCTGCCTC

COL7A1 exon 73 rs3R 50B
TCTCCTGGCC
612

TTCCTGCCTC

COL7A1 exon 73 TALEN L EA
TATTCCCGGG
622

CTCCCAGGCA

COL7A1 exon 73 TALEN L Het
TATTCCCGGG
622

CTCCCAGGCA

COL7A1 exon 73 TALEN R EA
TCTCCTGGCC
612

TTCCTGCCTC

COL7A1 exon 73 TALEN R Het
TCTCCTGGCC
612

TTCCTGCCTC

COL7A1 exon 73 TALEN L
TATTCCCGGG
622

EA/Het
CTCCCAGGCA

COL7A1 exon 73 TALEN R
TCTCCTGGCC
612

EA/Het
TTCCTGCCTC

HBB exon 1 TALEN L
TGGTGCATCT
623

GACTCCTGAG

HBB exon 1 TALEN R
TCACCTTGCC
624

CCACAGGGCA

PD-1 exon 1 TALEN L
TCCAGGCATG
625

CAGATCCCAC

PD-1 exon 1 TALEN R
TTGTAGCACC
626

GCCCAGACGA

TTR (forward (top) and
TGCTGTCCGA
639; 640

reverse (bottom)
GGCAGTCCTG

TCAGCAGCCT

TTCTGAACAC

TTR (forward (top) and
TGTCCGAGGC
641; 642

reverse (bottom)
AGTCCTGCCA

TCAGCAGCCT

TTCTGAACAC

TTR (forward (top) and
TCCGAGGCAG
643; 644

reverse (bottom)
TCCTGCCATC

TCAGCAGCCT

TTCTGAACAC

TTR (forward (top) and
TGTCCGAGGC
645; 646

reverse (bottom)
AGTCCTGCCA

TCATCAGCAG

CCTTTCTGAA

TTR (forward (top) and
TCCGAGGCAG
647; 648

reverse (bottom)
TCCTGCCATC

TCATCAGCAG

CCTTTCTGAA

TTR (forward (top) and
TCCGAGGCAG
649; 650

reverse (bottom)
TCCTGCCATC

TGTCATCAGC

AGCCTTTCTG

About 100,000 primary human neonatal epidermal keratinocytes were plated in EpiLife+Supplement S7 and transfected according to Example 3 with RNA encoding gene-editing proteins that target the sequences L: TGCCTGGTCCCTGTCTCCCT (SEQ ID NO: 615) and R: TGTCTTCTGGGCAGCATCTC (SEQ ID NO: 616), which were located approximately 75 bp from the Alpha-1-Antitrypsin (A1AT) start codon. Cells were gene edited, and gene-editing efficiency was measured as previously described. Results as shown in FIG. 46 demonstrate efficient gene-editing by the gene editing proteins.

About 100,000 primary human neonatal epidermal keratinocytes were plated in EpiLife+Supplement S7 and transfected according to Example 3 with RNA encoding gene-editing proteins that target the sequences L: TATTCCCGGGCTCCCAGGCA (SEQ ID NO: 622) and R: TCTCCTGGCCTTCCTGCCTC (SEQ ID NO: 612), which were located near the end of exon 73 of COL7A1. Cells were gene edited, and gene-editing efficiency was measured as previously described. Results as shown in FIG. 47 demonstrate efficient gene-editing by the gene editing proteins.

About 50,000 primary human neonatal epidermal keratinocytes were plated in EpiLife+Supplement S7 and transfected according to Example 3 with RNA encoding various gene-editing proteins that target the sequences L: TATTCCCGGGCTCCCAGGCA (SEQ ID NO: 622) and R: TCTCCTGGCCTTCCTGCCTC (SEQ ID NO: 612), which were located near the end of exon 73 of COL7A1. In particular, the gene-editing proteins comprised the endonuclease cleavage domain of FokI and variants thereof. The variants included a FokI variant with enhanced activity (S35P, K58E), a FokI heterodimer (i.e., L: Q103E, N113D, I116L; and R: E107K, H154R, I155K), and a combination thereof (i.e., L: S35P, K58E, Q103E, N113D, I116L; and R: S35P, K58E, E107K, H154R, I155K). Cells were gene edited, and gene-editing efficiency was measured as previously described. Results as shown in FIG. 48 demonstrate efficient gene-editing by the gene editing proteins.

About 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg RNA encoding the HBB exon 1 TALEN L and HBB exon 1 TALEN R gene-editing proteins (1 μg each). 48 hours after transfection, DNA was harvested and analyzed for gene editing (T7E1 assay; forward primer: gccaaggacaggtacggctgtcatc (SEQ ID NO: 627); reverse primer: cttgccatgagccttcaccttagggttg (SEQ ID NO: 628); product size: 518nt; predicted band sizes: 300nt, 218nt). Results as shown in FIG. 61 demonstrate efficient gene-editing by the gene editing proteins.

About 100,000 primary human neonatal epidermal keratinocytes (animal-protein free) were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg RNA encoding the PD1 exon 1 TALEN L and PD1 exon 1 TALEN R gene-editing proteins (1 μg each). After 48 hours, DNA was harvested and analyzed for gene editing (T7E1 assay; forward primer: tcctctgtctccctgtctctgtctctctctc (SEQ ID NO: 594); reverse primer: ggacttgggccaggggaggag (SEQ ID NO: 595); product size: 612nt; predicted band sizes: 349nt, 263nt). Results as shown in FIG. 62 demonstrate efficient gene-editing by the gene editing proteins.

Example 54: Transfection of Primary Human Keratinocytes with RNA Encoding GDF15, IFKB, KRT5, KRT14, SOD3, or hESM1

As shown in FIG. 49, 100,000 primary human neonatal epidermal keratinocytes were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg of RNA encoding hGDF15. Following transfection, the culture media was sampled at various time points and analyzed for hGDF15 levels using ELISA (R&D DGD150) according to manufacturer's instructions. FIG. 49 demonstrates that hGDF15 levels were upregulated in a time-dependent manner following transfection.

As shown in FIG. 50, 100,000 primary human neonatal epidermal keratinocytes were plated in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg of RNA encoding IFKB or an IFKB variant (S32A and S36A). Following transfection, the culture media was sampled at various time points and analyzed for IFKB levels using ELISA (Abcam, ab176644) according to manufacturer's instructions. FIG. 50 demonstrates increased levels IFKB or the IFKB variant following transfection.

As shown in FIG. 51, 100,000 primary human neonatal epidermal keratinocytes were plated in 6-well plates in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg of RNA encoding KRT5 or KRT14 (or GFP tagged versions of KRT5 or KRT14). Following transfection, GFP imaging was carried out to determine KRT5 or KRT14 expression. FIG. 51 demonstrates efficient transfection and increased levels KRT5-GFP or KRT14-GFP following transfection.

As shown in FIG. 52, 20,000 primary human neonatal epidermal keratinocytes were plated in 24-well plates in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 0.2 μg of RNA encoding S0D3. Following transfection, cells were fixed and stained for 24 hours with a 1:100 dilution of NBP2-38493 (Novus) rabbit anti human S0D3 primary antibody and a 1:1000 dilution of 488 donkey anti-rabbit secondary antibody. Results demonstrate efficient transfection and robust levels of SOD3 in transfected cells.

As shown in FIG. 60, 200,000 primary human neonatal epidermal keratinocytes (animal-protein free) were plated in 6-well plates in EpiLife+Supplement S7. Cells were transfected according to Example 3 with 2 μg RNA encoding hESM1. 52 hours after transfection, the culture medium was analyzed for ESM1 by ELISA (Abcam ab213776). Results demonstrate efficient transfection and robust levels of hESM1 in transfected cells.

Example 55: In Vivo Targeting of GDF15 (MIC1)

Studies were conducted to evaluate the responses of Zucker-obese (ZDF) rats to administration of RNA encoding hGDF15. The Zucker-obese rats (ZDF) are a well-studied obesity model that mimics many aspects of the human disease. Specifically, 8-10 weeks old male rats were used for this study. A total of 15 ZDF and 15 wildtype control Sprague-Dawley rats were tested, and the animals were assigned to study groups and treated as indicated in the Table below:

Dose

Group
Rat
Test

Level
Concentration
Volume^a
Number of

Group
Color
Species
Article
Route
(μg)
(μg/mL)
(μL)
Males

1
White
Sprague-
Control
Intradermal
0
0
50 per site;
5

Dawley

500 total

2
Yellow
Sprague-
FG-02 (i.e.,

10X (0.5 μg/
10
50 per site;
10

Dawley
RNA encoding

injection;

500 total

GDF15)

5 μg total)

3
Green
ZDF
Control

0
0
50 per site;
5

500 total

4
Blue
ZDF
FG-02 (i.e.,

10X (0.5 μg/
10
50 per site;
10

RNA encoding

injection;

500 total

GDF15)

5 μg total)

^aNote:

Total dose volume (μL/per animal) was constant

Groups 1 to 4 were dosed by intradermal (ID) injection. Each dose was administered on the back in ten ID injections of 50 μL/injection for a total of 500 uL per animal.

Each dose site was as follows:

embedded image

RNA or control was administered once weekly (Days 1, 8, and 15), with the first dose being on Day 1, and the last dose on Day 15. Animals were euthanized, without examination on Day 44.

Blood was collected from the rats according to the schedule below and used for serum chemistry and pharmacokinetics analysis:

Time Point (Study Week)
Serum Chemistry
PK

Acclimation (Week −2)
—
—

Dosing (Week 1)
2x
Day 1 (1x), 2 (1x), 3 (1x),

4 (1x), and 5 (1x)

Dosing (Week 2)
2x
Day 9^c

Dosing (Week 3)
2x
Day 16^c

Dosing (Week 4)
2x
Day 23

Dosing (Week 5)
2x
—

Dosing (Week 6)
2x
—

Dosing (Week 7)
1x
—

— = Not applicable

PK = Pharmacokinetics

x= Number of times procedure performed

^cSample collected 24 hours postdose

Serum chemistry analysis was carried out using an AU680 analyzer for the following: Albumin, Alkaline Phosphatase, Alanine Aminotransferase, Aspartate Aminotransferase, Total Bilirubin, Calcium, Total Cholesterol, Creatine Kinase, Creatinine, Glucose, Inorganic Phosphorus, Total Protein, Triglyceride, Sodium, Potassium, Chloride, Globulin, Albumin/Globulin Ratio, and Blood Urea Nitrogen. Results for changes in the serum levels of ALT, AST, total cholesterol, triglycerides, and glucose are provided in FIGS. 53-58. As shown in the FIG. 58, administration of RNA encoding GDF15 into ZDF rats resulted in reduced ALT, AST, and Glucose levels but elevated total cholesterol and triglyceride levels in the serum. These results suggest that diabetic obese animals treated with RNA encoding GDF15 exhibited improved liver health, as indicated by decreased serum levels of ALT and AST, improved (lower) blood glucose levels, and lipid mobilization, as indicated by increased serum cholesterol and triglyceride levels. These results therefore suggest that RNA encoding GDF15 may be used to treat, inter alia, diabetes, obesity, fatty liver, and other diseases that include liver inflammation, elevated blood glucose, and/or abnormal lipid metabolism.

The serum levels of GDF15 in the treated rats were confirmed as shown in FIG. 59. As shown, robust GDF15 levels were observed even at day 16 (i.e., day 15+24h) in both ZDF and control Sprague-dawley rats injected with RNA encoding GDF15.

EQUIVALENTS

Those skilled in the art will recognize, or be able to ascertain, using no more than routine experimentation, numerous equivalents to the specific embodiments described specifically herein. Such equivalents are intended to be encompassed in the scope of the following claims.

INCORPORATION BY REFERENCE

All patents and publications referenced herein are hereby incorporated by reference in their entireties.

	Number	Date	Country
	62509350	May 2017	US
	62376209	Aug 2016	US

	Number	Date	Country
Parent	16710813	Dec 2019	US
Child	17820473		US
Parent	15748132	Jan 2018	US
Child	16710813		US

NUCLEIC ACID PRODUCTS AND METHODS OF ADMINISTRATION THEREOF

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