Claims
- 1. A zinc finger protein that binds to a target site, said zinc finger protein comprising a first (F1), a second (F2), and a third (F3) zinc finger, ordered F1, F2, F3 from N-terminus to C-terminus, said target site comprising, in 3′ to 5′ direction, a first (S1), a second (S2), and a third (S3) target subsite, each target subsite having the nucleotide sequence GNN, wherein if S1 comprises GAA, F1 comprises the amino acid sequence QRSNLVR; if S2 comprises GAA, F2 comprises the amino acid sequence QSGNLAR; if S3 comprises GAA, F3 comprises the amino acid sequence QSGNLAR; if S1 comprises GAG, F1 comprises the amino acid sequence RSDNLAR; if S2 comprises GAG, F2 comprises the amino acid sequence RSDNLAR; if S3 comprises GAG, F3 comprises the amino acid sequence RSDNLTR; if S1 comprises GAC, F1 comprises the amino acid sequence DRSNLTR; if S2 comprises GAC, F2 comprises the amino acid sequence DRSNLTR; if S3 comprises GAC, F3 comprises the amino acid sequence DRSNLTR; if S1 comprises GAT, F1 comprises the amino acid sequence QSSNLAR; if S2 comprises GAT, F2 comprises the amino acid sequence TSGNLVR; if S3 comprises GAT, F3 comprises the amino acid sequence TSANLSR; if S1 comprises GGA, F1 comprises the amino acid sequence QSGHLAR; if S2 comprises GGA, F2 comprises the amino acid sequence QSGHLQR; if S3 comprises GGA, F3 comprises the amino acid sequence QSGHLQR; if S1 comprises GGG, F1 comprises the amino acid sequence RSDHLAR; if S2 comprises GGG, F2 comprises the amino acid sequence RSDHLSR; if S3 comprises GGG, F3 comprises the amino acid sequence RSDHLSR; if S1 comprises GGC, F1 comprises the amino acid sequence DRSHLRT; if S2 comprises GGC, F2 comprises the amino acid sequence DRSHLAR; if S1 comprises GGT, F1 comprises the amino acid sequence QSSHLTR; if S2 comprises GGT, F2 comprises the amino acid sequence TSGHLSR; if S3 comprises GGT, F3 comprises the amino acid sequence TSGHLVR; if S1 comprises GCA, F1 comprises the amino acid sequence QSGSLTR; if S2 comprises GCA, F2 comprises QSGDLTR; if S3 comprises GCA, F3 comprises QSGDLTR; if S1 comprises GCG, F1 comprises the amino acid sequence RSDDLTR; if S2 comprises GCG, F2 comprises the amino acid sequence RSDDLQR; if S3 comprises GCG, F3 comprises the amino acid sequence RSDDLTR; if S1 comprises GCC, F1 comprises the amino acid sequence ERGTLAR; if S2 comprises GCC, F2 comprises the amino acid sequence DRSDLTR; if S3 comprises GCC, F3 comprises the amino acid sequence DRSDLTR; if S1 comprises GCT, F1 comprises the amino acid sequence QSSDLTR; if S2 comprises GCT, F2 comprises the amino acid sequence QSSDLTR; if S3 comprises GCT, F3 comprises the amino acid sequence QSSDLQR; if S1 comprises GTA, F1 comprises the amino acid sequence QSGALTR; if S2 comprises GTA, F2 comprises the amino acid sequence QSGALAR; if S1 comprises GTG, F1 comprises the amino acid sequence RSDALTR; if S2 comprises GTG, F2 comprises the amino acid sequence RSDALSR; if S3 comprises GTG, F3 comprises the amino acid sequence RSDALTR; if S1 comprises GTC, F1 comprises the amino acid sequence DRSALAR; if S2 comprises GTC, F2 comprises the amino acid sequence DRSALAR; and if S3 comprises GTC, F3 comprises the amino acid sequence DRSALAR.
- 2. The zinc finger protein of claim 1, wherein S1 comprises GAA and F1 comprises the amino acid sequence QRSNLVR.
- 3. The zinc finger protein of claim 1, wherein S2 comprises GAA and F2 comprises the amino acid sequence QSGNLAR.
- 4. The zinc finger protein of claim 1, wherein S3 comprises GAA and F3 comprises the amino acid sequence QSGNLAR.
- 5. The zinc finger protein of claim 1, wherein S1 comprises GAG and F1 comprises the amino acid sequence RSDNLAR.
- 6. The zinc finger protein of claim 1, wherein S2 comprises GAG and F2 comprises the amino acid sequence RSDNLAR.
- 7. The zinc finger protein of claim 1, wherein S3 comprises GAG and F3 comprises the amino acid sequence RSDNLTR.
- 8. The zinc finger protein of claim 1, wherein S1 comprises GAC and F1 comprises the amino acid sequence DRSNLTR.
- 9. The zinc finger protein of claim 1, wherein S2 comprises GAC and F2 comprises the amino acid sequence DRSNLTR.
- 10. The zinc finger protein of claim 1, wherein S3 comprises GAC and F3 comprises the amino acid sequence DRSNLTR.
- 11. The zinc finger protein of claim 1, wherein S1 comprises GAT and F1 comprises the amino acid sequence QSSNLAR.
- 12. The zinc finger protein of claim 1, wherein S2 comprises GAT and F2 comprises the amino acid sequence TSGNLVR.
- 13. The zinc finger protein of claim 1, wherein S3 comprises GAT and F3 comprises the amino acid sequence TSANLSR.
- 14. The zinc finger protein of claim 1, wherein S1 comprises GGA and F1 comprises the amino acid sequence QSGHLAR.
- 15. The zinc finger protein of claim 1, wherein S2 comprises GGA and F2 comprises the amino acid sequence QSGHLQR.
- 16. The zinc finger protein of claim 1, wherein S3 comprises GGA and F3 comprises the amino acid sequence QSGHLQR.
- 17. The zinc finger protein of claim 1, wherein S1 comprises GGG and F1 comprises the amino acid sequence RSDHLAR.
- 18. The zinc finger protein of claim 1, wherein S2 comprises GGG and F2 comprises the amino acid sequence RSDHLSR.
- 19. The zinc finger protein of claim 1, wherein S3 comprises GGG and F3 comprises the amino acid sequence RSDHLSR.
- 20. The zinc finger protein of claim 1, wherein S1 comprises GGC and F1 comprises the amino acid sequence DRSHLTR.
- 21. The zinc finger protein of claim 1, wherein S2 comprises GGC and F2 comprises the amino acid sequence DRSHLAR.
- 22. The zinc finger protein of claim 1, wherein S11 comprises GGT and F1 comprises the amino acid sequence QSSHLTR.
- 23. The zinc finger protein of claim 1, wherein S2 comprises GGT and F2 comprises the amino acid sequence TSGHLSR.
- 24. The zinc finger protein of claim 1, wherein S3 comprises GGT and F3 comprises the amino acid sequence TSGHLVR.
- 25. The zinc finger protein of claim 1, wherein S1 comprises GCA and F1 comprises the amino acid sequence QSGSLTR.
- 26. The zinc finger protein of claim 1, wherein S2 comprises GCA and F2 comprises the amino acid sequence QSGDLTR.
- 27. The zinc finger protein of claim 1, wherein S3 comprises GCA and F3 comprises the amino acid sequence QSGDLTR.
- 28. The zinc finger protein of claim 1, wherein S1 comprises GCG and F1 comprises the amino acid sequence RSDDLTR.
- 29. The zinc finger protein of claim 1, wherein S2 comprises GCG and F2 comprises the amino acid sequence RSDDLQR.
- 30. The zinc finger protein of claim 1, wherein S3 comprises GCG and F3 comprises the amino acid sequence RSDDLTR.
- 31. The zinc finger protein of claim 1, wherein S1 comprises GCC and F1 comprises the amino acid sequence ERGTLAR.
- 32. The zinc finger protein of claim 1, wherein S2 comprises GCC and F2 comprises the amino acid sequence DRSDLTR.
- 33. The zinc finger protein of claim 1, wherein S3 comprises GCC and F3 comprises the amino acid sequence DRSDLTR.
- 34. The zinc finger protein of claim 1, wherein S1 comprises GCT and F1 comprises the amino acid sequence QSSDLTR.
- 35. The zinc finger protein of claim 1, wherein S2 comprises GCT and F2 comprises the amino acid sequence QSSDLTR.
- 36. The zinc finger protein of claim 1, wherein S3 comprises GCT and F3 comprises the amino acid sequence QSSDLQR.
- 37. The zinc finger protein of claim 1, wherein S1 comprises GTA and F1 comprises the amino acid sequence QSGALTR.
- 38. The zinc finger protein of claim 1, wherein S2 comprises GTA and F2 comprises the amino acid sequence QSGALAR.
- 39. The zinc finger protein of claim 1, wherein S1 comprises GTG and F1 comprises the amino acid sequence RSDALTR.
- 40. The zinc finger protein of claim 1, wherein S2 comprises GTG and F2 comprises the amino acid sequence RSDALSR.
- 41. The zinc finger protein of claim 1, wherein S3 comprises GTG and F3 comprises the amino acid sequence RSDALTR.
- 42. The zinc finger protein of claim 1, wherein S1 comprises GTC and F1 comprises the amino acid sequence DRSALAR.
- 43. The zinc finger protein of claim 1, wherein S2 comprises GTC and F2 comprises the amino acid sequence DRSALAR.
- 44. The zinc finger protein of claim 1, wherein S3 comprises GTC and F3 comprises the amino acid sequence DRSALAR.
- 45. A polypeptide comprising a zinc finger protein according to claim 1.
- 46. A polypeptide according to claim 45, further comprising at least one functional domain.
- 47. A polynucleotide encoding a zinc finger protein according to claim 1.
- 48. A polynucleotide encoding a polypeptide according to claim 45.
- 49. A polynucleotide encoding a polypeptide according to claim 46.
CROSS-REFERENCES TO RELATED APPLICATIONS
[0001] The present application is a continuation-in-part of copending U.S. patent application Ser. No. 09/535,008, filed Mar. 23, 2000, which application claims the benefit of U.S. provisional applications No. 60/126,238, filed Mar. 24, 1999, No. 60/126,239 filed Mar. 24, 1999, No. 60/146,595 filed Jul. 30, 1999 and No. 60/146,615 filed Jul. 30, 1999. The present application is also a continuation-in-part of copending U.S. patent application Ser. No. 09/716,637, filed Nov. 20, 2000. The disclosures of all of the aforementioned applications are hereby incorporated by reference in their entireties for all purposes.
Provisional Applications (4)
|
Number |
Date |
Country |
|
60126238 |
Mar 1999 |
US |
|
60126239 |
Mar 1999 |
US |
|
60146595 |
Jul 1999 |
US |
|
60146615 |
Jul 1999 |
US |
Continuation in Parts (2)
|
Number |
Date |
Country |
Parent |
09535008 |
Mar 2000 |
US |
Child |
09989994 |
Nov 2001 |
US |
Parent |
09716637 |
Nov 2000 |
US |
Child |
09989994 |
Nov 2001 |
US |