Claims
- 1. A mammalian cell transformed in vitro with a ribozyme as depicted by FIG. 1, FIG. 2, FIG. 3, FIG. 4, or FIG 5.
- 2. A mammalian cell transformed in vitro with a ribozyme as depicted by FIG. 4.
- 3. A ribozyme containing expression product of a cell as defined by claim 1.
- 4. A recombinant expression vector containing a promoter, a gene encoding a ribozyme as depicted by FIGS. 1-5, a transcriptional terminator and a poly A addition site.
- 5. A mammalian cell transformed in vitro with a vector as defined by claim 4.
- 6. A HIV-I infected HeLa cell transformed with a ribozyme as depicted by FIG. 4.
- 7. An HIV-I infected HeLa cell transformed with a ribozyme as depicted by FIG. 1, FIG. 2, or FIG. 3.
- 8. An expression product of a HeLa cell infected with HIV-I and transformed with a ribozyme as depicted by FIG. 4, said expression product containing said ribozyme.
- 9. An HIV-I infected HeLa cell transformed with a ribozyme as depicted by FIG. 4, said ribozyme cleaving HIV-I gag transcripts in vitro.
- 10. A recombinant expression vector as defined by claim 4 containing a gene encoding a ribozyme as depicted by FIG. 4.
Parent Case Info
This is a division of application Ser. No. 401,613 filed Aug. 31, 1989, now abandoned, which is a continuation-in-part of pending application Ser. No. 369,489 filed Jun. 21, 1989 now U.S. Pat. No. 5,144,019.
Government Interests
This invention was made with government support under Grant No. J.R. NIAID R01 AI29329 awarded by the National Institutes of Health. The government has certain rights in the invention.
Non-Patent Literature Citations (4)
Entry |
Barinaga Science 262: 1512-1514 (1993). |
Johnston et al. Science 260: 1286-1293 (1993). |
Gerlach et al. J. Cell. Biochem. Suppl. 12C, 239, 1988. |
Rossi et al. J. Cell. Biochem. Suppl. 13B, G329, 1989. |
Divisions (1)
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401613 |
Aug 1989 |
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Continuation in Parts (1)
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369489 |
Jun 1989 |
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