Claims
- 1. An isolated heterodimeric receptor complex comprising two soluble receptor subunits, wherein the first soluble receptor subunit comprises a soluble receptor polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:6, and wherein the second soluble receptor subunit comprises a soluble IL-2Rγ receptor polypeptide (SEQ ID NO:4).
- 2. The isolated heterodimeric soluble complex according to claim 1, wherein the soluble receptor complex further comprises an affinity tag, label, chemical moiety, toxin, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, toxin, cytotoxic molecule or an immunoglobulin Fc domain.
- 3. The isolated heterodimeric receptor complex according to claim 1, wherein the heterodimeric receptor complex binds a ligand comprising a polypeptide of SEQ ID NO:10.
- 4. The isolated heterodimeric receptor complex according to claim 1, wherein the heterodimeric receptor complex antagonizes the activity of a ligand comprising a polypeptide of SEQ ID NO:10.
- 5. An isolated heterodimeric receptor complex comprising two soluble receptor subunits, wherein the first soluble receptor subunit consists of a soluble receptor polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:6, and the second receptor subunit consists of a soluble receptor polypeptide comprising soluble IL-2Rγ receptor polypeptide (SEQ ID NO:4).
- 6. The isolated heterodimeric receptor complex according to claim 5, wherein the heterodimeric receptor complex binds a ligand comprising a polypeptide of SEQ ID NO:10.
- 7. The isolated heterodimeric receptor complex according to claim 5, wherein at least one of the soluble receptor subunits further comprises an affinity tag, label, chemical moiety, toxin, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, toxin, cytotoxic molecule or an immunoglobulin Fc domain.
- 8. The isolated heterodimeric receptor complex according to claim 5, wherein the heterodimeric receptor complex antagonizes the activity of a ligand comprising a polypeptide of SEQ ID NO:10.
- 9. An isolated heterodimeric receptor complex consisting of two soluble receptor subunits, wherein the first soluble receptor subunit consists of a soluble receptor polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:6, and the second receptor subunit consists of a soluble receptor polypeptide comprising soluble IL-2Rγ receptor polypeptide (SEQ ID NO:4).
- 10. The isolated heterodimeric receptor complex according to claim 9, wherein the heterodimeric receptor complex binds a ligand comprising a polypeptide of SEQ ID NO:10.
- 11. The isolated heterodimeric receptor complex according to claim 9, wherein ax least one of the soluble receptor subunits further comprises an affinity rag, label, chemical moiety, toxin, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, toxin, cytotoxic molecule or an immunoglobulin Fc domain.
- 12. The isolated heterodimeric receptor complex according to claim 9, wherein at least one of the soluble receptor subunits further comprises a transmembrane domain from a cytokine receptor.
- 13. The isolated heterodimeric receptor complex according to claim 12, wherein the transmembrane domain is from a Class I cytokine receptor.
- 14. The isolated heterodimeric receptor complex according to claim 9, wherein at least one of the soluble receptor subunits further comprises a transmembrane domain and an intracellular domain from a cytokine receptor.
- 15. The isolated heterodimeric receptor complex according to claim 14, wherein the intracellular domain is from a Class I cytokine receptor.
- 16. The isolated heterodimeric receptor complex according to claim 9, wherein both of the soluble receptor subunits further comprise a transmembrane domain from a cytokine receptor.
- 17. The isolated heterodimeric receptor complex according to claim 16, wherein the transmembrane domain is from a Class I cytokine receptor.
- 18. The isolated heterodimeric receptor complex according to claim 9, wherein both of the soluble receptor subunits further comprise a transmembrane domain and an intracellular domain from a cytokine receptor.
- 19. The isolated heterodimeric receptor complex according to claim 18, wherein the intracellular domain is from a Class I cytokine receptor.
- 20. The isolated heterodimeric receptor complex according to claim 9, wherein the heterodimeric receptor complex antagonizes the activity of a ligand comprising a polypeptide of SEQ ID NO:10.
- 21. An isolated multimeric soluble receptor complex comprising soluble receptor subunits, wherein at least one of soluble receptor subunits comprises a soluble receptor polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:6, and wherein at least one other of the soluble receptor subunits comprises a soluble receptor polypeptide comprising soluble IL-2Rγ receptor polypeptide (SEQ ID NO:4).
- 22. The isolated multimeric soluble receptor complex according to claim 21, wherein the multimeric soluble receptor complex binds a ligand comprising a polypeptide of SEQ ID NO:10.
- 23. The isolated multimeric soluble receptor complex according to claim 21, wherein at least one of the soluble receptor subunits further comprises an affinity tag, label, chemical moiety, toxin, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, toxin, cytotoxic molecule or an immunoglobulin Fc domain.
- 24. The isolated multimeric soluble receptor complex according to claim 21, wherein the multimeric soluble receptor complex antagonizes the activity of a ligand comprising a polypeptide of SEQ ID NO:10.
- 25. The isolated multimeric soluble receptor complex according to claim 21, wherein at least one of soluble receptor subunits consists of a soluble receptor polypeptide comprising a sequence of amino acid residues as shown in SEQ ID NO:6, and wherein at least one other of the soluble receptor subunits consists of a soluble receptor polypeptide comprising soluble IL-2Rγ receptor polypeptide (SEQ ID NO:4).
- 26. The isolated multimeric soluble receptor complex according to claim 25, wherein the multimeric soluble receptor complex binds a ligand comprising a polypeptide of SEQ ID NO:10.
- 27. The isolated multimeric soluble receptor complex according to claim 25, wherein at least one of the soluble receptor subunits further comprises an affinity tag, label, chemical moiety, toxin, biotin/avidin label, radionuclide, enzyme, substrate, cofactor, inhibitor, fluorescent marker, chemiluminescent marker, toxin, cytotoxic molecule or an immunoglobulin Fc domain.
- 28. The isolated multimeric soluble receptor complex according to claim 25, wherein the multimeric receptor complex antagonizes the activity of a ligand comprising a polypeptide of SEQ ID NO:10.
REFERENCE TO RELATED APPLICATIONS
This application is related to Provisional Application No. 60/194,731, filed on Apr. 5, 2000. This application is also related to Provisional Application No. 60/222,121, filed on Jul. 28, 2000. Under 35 U.S.C. §119(e)(1), this application claims benefit of said Provisional Applications.
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|
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|
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