Claims
- 1. An isolated protein selected from the group consisting ofa. protein comprising amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:8, SEQ ID NO:89 and SEQ ID NO:95; and, b. a flea protein comprising a homologue of a protein comprising an amino acid seuence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:8, SEQ ID NO:89 and SEQ ID NO:95; wherein said homologe has at least a 6 contiguous amino acid portion of a sequence selected from the group consisfing ofSEQ ID NO:2, SEQ ID NO:8, SEQ ID NO:89 and SEQ ID NO:95.
- 2. The protein of claim 1, wherein said protein comprises an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:8, SEQ ID NO:89 and SEQ ID NO:95.
- 3. A method to identify a compound capable of inhibiting flea protease activity, said method comprising:(a) contacting an isolated flea protease protein comprising an amino acid sequence selected from the group consisting of SEQ ID NO:2 SEQ ID NO:8, SEQ ID NO:89 and SEQ ID NO:95 with a putative inhibitory compound under conditions in which, in the absence of said compound, said protein has proteolytic activity; and (b) determining if said putative inhibitory compound inhibits said activity.
CROSS-REFERENCE TO RELATED APPLICATIONS
The present invention is a divisional of U.S. patent application Ser. No. 08/749,699, filed Nov. 15, 1996, which is a continuation-in-part of U.S. patent application Ser. Nos. 08/484,211 (issued as U.S. Pat. No. 5,972,645 on Oct. 26, 1999), 08/482,130 (issued as U.S. Pat. No. 5,962,257 on Oct. 5, 1999), 08/485,443, and 08/485,455 (issued as U.S. Pat. No. 5,712,143 on Jan. 27, 1998), each of which was filed on Jun. 7, 1995 and each of which is a continuation-in-part of U.S. Patent Application Serial No. 08/326,773, filed Oct. 18, 1994 which issued as U.S. Pat. No. 5,766,609 on Jun. 16, 1998, and which is a continuation-in-part of U.S. patent application Ser. No. 07/806,482, filed Dec. 13, 1991, which issued as U.S. Pat. No. 5,356,622 on Oct. 18, 1994. The present application is also a continuation-in-part of U.S. patent application Ser. No. 08/326,773, ibid., as well as of WO 96/11706, the international publication date of which is Apr. 25, 1996. WO 96/11706 claims priority to U.S. patent application Ser. Nos. 08/326,773, 08/484,211, 08/482,130, 08/485,443 and 08/485,455, ibid. Each of the applications referred to in this section is incorporated herein by reference in its entirety.
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Kind |
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Jan 1998 |
|
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|
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Number |
Date |
Country |
WO9611706 |
Apr 1996 |
WO |
Non-Patent Literature Citations (1)
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Continuation in Parts (6)
|
Number |
Date |
Country |
Parent |
08/484211 |
Jun 1995 |
US |
Child |
08/749699 |
|
US |
Parent |
08/482130 |
Jun 1995 |
US |
Child |
08/484211 |
|
US |
Parent |
08/485443 |
Jun 1995 |
US |
Child |
08/482130 |
|
US |
Parent |
08/485455 |
Jun 1995 |
US |
Child |
08/485443 |
|
US |
Parent |
08/326773 |
Oct 1994 |
US |
Child |
08/484211 |
Jun 1995 |
US |
Parent |
07/806482 |
Dec 1991 |
US |
Child |
08/482130 |
Jun 1995 |
US |