Claims
- 1. An apparatus for fabrication by multi-photon excitation, comprising a photon source in connection with 4 pi optics, wherein the 4 pi optics comprises a first high NA lens located above a movable stage and a second high NA lens located beneath the movable stage.
- 2. The apparatus of claim 1, further comprising a second movable stage located between the photon source and the first high NA lens.
- 3. An apparatus for fabrication by multi-photon excitation, comprising a photon source in connection with near field optics, wherein the near field optics comprises fiber optic couplers in conjunction with a near field fiber optic element for fabrication.
- 4. The apparatus of claim 3, further comprising an optical microscope as an imaging element.
- 5. The apparatus of claim 3, wherein the optical element is coupled with a multiple-barrel pipette.
- 6. The apparatus of claim 5, wherein the optical element is disposed within one of the pipette barrels.
- 7. A device for fabrication by multi-photon excitation at remote locations, comprising
a photon source connected to a first end of single mode optical fiber via a fiber optic coupler and a group velocity delay self-phase modulation compensator; wherein the second end of the optical fiber comprises a lens for multi-photon fabrication.
- 8. The device of claim 7, wherein the optical fiber is housed in a catheter.
- 9. The device of claim 8, wherein the catheter further houses at least one reagent tube for dispensing reagent at the site of fabrication.
- 10. The device of claim 9, wherein the second end of the optical fiber further comprises a cowling.
- 11. The device of claim 7, further comprising a scanning galvometer.
- 12. The device of claim 7, further comprising a post laser prism or a grating pair.
- 13. A method for protein or peptide crosslinking, comprising:
providing a photoactivatable precursor composition comprising at least two protein molecules, at least tow peptide molecules, or a protein molecule and a peptide molecules; activating the precursor composition using multi-photon excitation in at least one first location in the precursor composition to form at least one first portion of a construct, wherein the at least one first portion has dimensions in the X-Y directions of less than about 300 nanometers, and wherein the at least one first portion is covalently linked to the surface.
- 14. The method of claim 13, wherein the at least one first portion has a dimension in the Z direction of less than about 500 nanometers.
- 15. The method of claim 13, wherein the at least one portion has dimensions in the X-Y direction of less than about 250 nanometers.
- 16. The method of claim 15, wherein the at least one first portion has a dimension in the Z direction in the range of less than about 300 nanometers.
- 17. The method of claim 15, wherein the at least one portion has a dimension in the Z direction of less than about 100 nanometers.
- 18. The method of claim 7, wherein the at least one first portion has dimensions in the X, Y, and Z directions of less than about 50 nanometers.
- 19. The method of claim 7, wherein multi-photon excitation occurs by three-photon excitation to effect the pi to pi* transition of proteins or peptides containing aromatic residues, followed by one- or two-photon excitation to excite the chromophore above the ionization limit.
- 20. A method for in vitro tissue modification, comprising:
introducing a precursor composition to a tissue to be modified; and activating the precursor composition using multi-photon excitation in at least one first location in the precursor composition to form at least one first portion of a construct, wherein the at least one first portion has dimensions in the X-Y directions of less than about 300 nanometers to modify the tissue.
- 21. The method of claim 20, wherein the at least one first portion has a dimension in the Z direction of less than about 500 nanometers.
- 22. The method of claim 20, wherein the at least one portion has dimensions in the X-Y direction of less than about 250 nanometers.
- 23. The method of claim 22, wherein the at least one first portion has a dimension in the Z direction in the range of less than about 300 nanometers.
- 24. The method of claim 22, wherein the at least one portion has a dimension in the Z direction of less than about 100 nanometers.
- 25. The method of claim 20, wherein the at least one first portion has dimensions in the X, Y, and Z directions of less than about 50 nanometers
- 26. The method of claim 20, wherein tissue modification is effected at up to about 350 microns deep into soft tissue.
- 27. The method of claim 20, wherein modification is effected at a remote location, wherein activation is via an optical fiber housed within a catheter, and wherein reagent introduction is via a lumen of or within a catheter.
CROSS-REFERENCE TO RELATED APPLICATIONS
[0001] This application is a continuation of U.S. application Ser. No. 09/295,992, filed on Apr. 21, 1999, which is incorporated herein in its entirety.
Provisional Applications (2)
|
Number |
Date |
Country |
|
60082575 |
Apr 1998 |
US |
|
60112797 |
Dec 1998 |
US |
Continuations (1)
|
Number |
Date |
Country |
Parent |
09295992 |
Apr 1999 |
US |
Child |
09947022 |
Sep 2001 |
US |