Claims
- 1. An ex vivo method of promoting proliferation of a hematopoietic stem cell cell comprising contacting said cell with an amount of a polypeptide, wherein said polypeptide comprises an amino acid sequence at least 95% identical to the amino acid of SEQ ID NO: 13, 32 or 34 or the mature protein coding portion thereof and exhibits stem cell growth factor activity, and wherein said amount is effective to promote proliferation of said cell.
- 2. An ex vivo method of promoting proliferation of a hematopoietic stem cell comprising contacting said cell with an amount of a polypeptide, wherein the polypeptide is encoded by a polynucleotide that hybridizes to the complement of the nucleotide sequence of SEQ ID NO: 12, or the mature protein coding portion thereof, under the following stringent conditions: a final wash of 0.1×SSC/0.1% SDS at 68° C.,wherein the amount is effective to promote proliferation of said cell.
- 3. An ex vivo method of maintaining survival of a hemoatropoietic stem cell comprising contacting said cell with an amount of a polypeptide, wherein said polypeptide comprises an amino acid sequence at least 95% identical to the amino acid of SEQ ID NO: 13, 32 or 34 or the mature protein coding portion thereof and exhibits stem cell growth factor activity, and wherein said amount is effective to maintain survival of said cell.
- 4. An ex vivo method of maintaining survival of a hematopoietic stem cell comprising contacting said cell with an amount of a polypeptide, wherein the polypeptide is encoded by a polynucleotide that hybridizes to the complement of the nucleotide sequence of SEQ ID NO: 12, or the mature protein coding portion thereof, under the following stringent conditions: a final wash of 0.1×SSC/0.1% SDS at 68° C.,wherein the amount is effective to maintain survival of said cell.
- 5. The method of claim 1 or 3, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 13, or the mature protein coding portion thereof.
- 6. The method of claim 1 or 3, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 32, or the mature protein coding portion thereof.
- 7. The method of claim 1 or 3, wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 34, or the mature protein coding portion thereof.
1. CROSS REFERENCE TO RELATED APPLICATIONS
This application claims priority of U.S. Provisional Application Ser. No. 60/282,397 filed Apr. 5, 2001, and U.S. Provisional Application Ser. No. 60/215,733, filed Jun. 28, 2000, and U.S. Provisional Application Ser. No. 60/266,614 filed Feb. 5, 2001, now abandoned, is a continuation-in-part application of U.S. application Ser. No. 09/757,562 filed Jan. 9, 2001, now abandoned, entitled “Methods and Materials Relating to Novel Stem Cell Growth Factor-Like Polypeptides and Polynucleotides”, which in turn is a continuation application of U.S. application Ser. No. 09/543,774 filed Apr. 5, 2000, now abandoned, entitled “Methods and Materials Relating to Novel Stem Cell Growth Factor-Like Polypeptides and Polynucleotides”, which in turn is a continuation-in-part application of U.S. application Ser. No. 09/496,914 filed Feb. 03, 2000, now abandoned, entitled “Novel Contigs Obtained from Various Libraries”, all of which are incorporated herein by reference in their entirety.
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Number |
Date |
Country |
WO 9849302 |
Nov 1998 |
WO |
WO 0056755 |
Sep 2000 |
WO |
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Provisional Applications (3)
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Number |
Date |
Country |
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60/282397 |
Apr 2001 |
US |
|
60/266614 |
Feb 2001 |
US |
|
60/215733 |
Jun 2000 |
US |
Continuations (1)
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Number |
Date |
Country |
Parent |
09/543774 |
Apr 2000 |
US |
Child |
09/757562 |
|
US |
Continuation in Parts (2)
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Number |
Date |
Country |
Parent |
09/757562 |
Jan 2001 |
US |
Child |
09/894912 |
|
US |
Parent |
09/496914 |
Feb 2000 |
US |
Child |
09/543774 |
|
US |