Claims
- 1. A filamentous bacteriophage particle displaying on its surface a binding molecule which has a binding domain able to bind target epitope or antigen, wherein the binding domain of the binding molecule consists of a dAb fragment, the particle containing nucleic acid with a nucleotide sequence encoding the binding molecule.
- 2. A filamentous bacteriophage particle according to claim 1 wherein the binding molecule is synthetic.
- 3. A filamentous bacteriophage particle according to claim 2 wherein the nucleotide sequence encoding the binding molecule is provided by combining unrearranged V segments with D and J segments.
- 4. A filamentous bacteriophage particle according to claim 1 wherein the nucleotide sequence encoding the binding molecule is derived by in vitro mutagenesis of an existing antibody coding sequence or pre-existing phage antibodies.
- 5. A filamentous bacteriophage particle according to claim 1 wherein the nucleotide sequence encoding the binding molecule is derived from a peripheral blood lymphocyte.
- 6. A filamentous bacteriophage particle according to claim 1 wherein said nucleic acid is comprised in a phagemid genome within the filamentous bacteriophage particle.
- 7. A filamentous bacteriophage particle according to any one of claims 1 to 6, which is in a population of filamentous bacteriophage particles displaying a population of said binding molecules having a range of binding specificities.
- 8. A population of filamentous bacteriophage particles according to claim 7 displaying a population of said binding molecules having a range of binding specificities.
- 9. A method for producing a binding molecule specific for a particular target epitope or antigen, which method comprises the steps of:
producing a population of filamentous bacteriophage particles displaying at their surface a population of binding molecules, wherein each binding molecule in the population of binding molecules has a binding domain and the population of binding molecules has a range of binding specificities, wherein the binding domain of the binding molecules consists of a dAb fragment, and wherein each filamentous bacteriophage particle contains nucleic acid with a nucleotide sequence encoding the binding molecule expressed from the nucleic acid and displayed by the particle at its surface; selecting for a filamentous bacteriophage particle displaying a binding molecule with a desired specificity by contacting the population of filamentous bacteriophage particles with a target epitope or antigen so that individual binding molecules displayed on filamentous bacteriophage particles with the desired specificity bind to said target epitope or antigen.
- 10. A method according to claim 9 wherein the binding molecules are synthetic.
- 11. A method according to claim 10 wherein nucleotide sequences encoding the binding molecules are provided by combining unrearranged V segments with D and J segments.
- 12. A method according to claim 9 wherein the nucleotide sequences encoding the binding molecules are derived by in vitro mutagenesis of an existing antibody coding sequence or pre-existing phage antibodies.
- 13. A method according to claim 9 wherein the nucleotide sequences encoding the binding molecules are derived from peripheral blood lymphocytes.
- 14. A method according to claim 9 wherein said nucleic acid is comprised in a phagemid genome within each filamentous bacteriophage particle.
- 15. A method according to any one of claims 9 to 14 additionally comprising
separating bound filamentous bacteriophage particles from the target epitope or antigen.
- 16. A method according to claim 15 additionally comprising
recovering separated filamentous bacteriophage particles displaying a binding molecule with the desired specificity.
- 17. A method according to claim 16 additionally comprising
producing in a recombinant system by expression from nucleic acid derived from said separated particles the binding molecule, or a fragment or derivative thereof with binding specificity for the target epitope or antigen, separate from filamentous bacteriophage particles.
Priority Claims (5)
Number |
Date |
Country |
Kind |
9015198.6 |
Jul 1990 |
GB |
|
9022845.3 |
Oct 1990 |
GB |
|
9024503.6 |
Nov 1990 |
GB |
|
9104744.9 |
Mar 1991 |
GB |
|
9110549.4 |
May 1991 |
GB |
|
Parent Case Info
[0001] This is a divisional of U.S. application Ser. No. 09/726,219 filed Nov. 28, 2000, which in turn is a continuation of U.S. application Ser. No. 08/484,893, filed Jun. 7, 1995 which issued as U.S. Pat. No. 6,172,197 on Jan. 9, 2001, which in turn is a continuation of U.S. application Ser. No. 07/971,857, filed Jan. 8, 1993 (now U.S. Pat. No. 5,969,108), which in turn is the U.S. national phase of PCT/GB91/01134, filed 10 Jul. 1991.
Divisions (1)
|
Number |
Date |
Country |
Parent |
09726219 |
Nov 2000 |
US |
Child |
10803622 |
Mar 2004 |
US |
Continuations (2)
|
Number |
Date |
Country |
Parent |
08484893 |
Jun 1995 |
US |
Child |
09726219 |
Nov 2000 |
US |
Parent |
07971857 |
Jan 1993 |
US |
Child |
08484893 |
Jun 1995 |
US |