Claims
- 1. A compound 8 to 50 nucleobases in length targeted to a nucleic acid molecule encoding Transforming growth factor beta receptor II, wherein said compound specifically hybridizes with said nucleic acid molecule encoding Transforming growth factor beta receptor II and inhibits the expression of Transforming growth factor beta receptor II.
- 2. The compound of claim 1 which is an antisense oligonucleotide.
- 3. The compound of claim 2 wherein the antisense oligonucleotide has a sequence comprising SEQ ID NO: 21, 22, 27, 28, 33, 34, 41, 42, 44, 47, 49, 50, 53, 56, 57, 61, 63, 65, 66, 67, 68, 72, 73, 74, 76, 78, 80, 87, 91, 93, 94, 20, 23, 24, 25, 29, 30, 31, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 121, 122, 123, 124, 125, 127, 128, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 143, 144, 145, 146, 147, 149, 150, 152, 153, 154, 155, 156, 158, 160 or 161.
- 4. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified internucleoside linkage.
- 5. The compound of claim 4 wherein the modified internucleoside linkage is a phosphorothioate linkage.
- 6. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified sugar moiety.
- 7. The compound of claim 6 wherein the modified sugar moiety is a 2′-O-methoxyethyl sugar moiety.
- 8. The compound of claim 2 wherein the antisense oligonucleotide comprises at least one modified nucleobase.
- 9. The compound of claim 8 wherein the modified nucleobase is a 5-methylcytosine.
- 10. The compound of claim 2 wherein the antisense oligonucleotide is a chimeric oligonucleotide.
- 11. A compound 8 to 50 nucleobases in length which specifically hybridizes with at least an 8-nucleobase portion of an active site on a nucleic acid molecule encoding Transforming growth factor beta receptor II.
- 12. A composition comprising the compound of claim 1 and a pharmaceutically acceptable carrier or diluent.
- 13. The composition of claim 12 further comprising a colloidal dispersion system.
- 14. The composition of claim 12 wherein the compound is an antisense oligonucleotide.
- 15. A method of inhibiting the expression of Transforming growth factor beta receptor II in cells or tissues comprising contacting said cells or tissues with the compound of claim 1 so that expression of Transforming growth factor beta receptor II is inhibited.
- 16. A method of treating an animal having a disease or condition associated with Transforming growth factor beta receptor II comprising administering to said animal a therapeutically or prophylactically effective amount of the compound of claim 1 so that expression of Transforming growth factor beta receptor II is inhibited.
- 17. The method of claim 16 wherein the disease or condition is a hyperproliferative disorder.
- 18. The method of claim 17 wherein the hyperproliferative disorder is cancer.
- 19. The method of claim 18 wherein the cancer is lung, liver, bone, breast, cervical, colon, gastric, pancreatic, esophageal, or hematopoetic cancer.
- 20. The method of claim 16 wherein the disease or condition involves activation of the immune system.
INTRODUCTION
[0001] This application is a continuation of U.S. Ser. No. 09/888,361 filed Jun. 21, 2001, which is herein incorporated by reference in its entirety.
Continuations (1)
|
Number |
Date |
Country |
Parent |
09888361 |
Jun 2001 |
US |
Child |
10705715 |
Nov 2003 |
US |