Patent Grant
11040057
The present application claims priority to Korean Patent Application No. KR 10-2016-0081914, filed Jun. 29, 2016, the contents of which are herein incorporated by reference in their entireties.
The present invention provides compounds or agents that potentiate siRNA cellular entry or activity. The invention further provides methods for identifying such compounds or agents.
The contents of the text file submitted electronically herewith are incorporated herein by reference in their entirety: A computer readable format copy of the Sequence Listing (filename: OLX-004PC-Sequence Listing; date recorded: Jun. 27, 2017; file size: 33 KB).
RNA interference (RNAi) provides the ability to inhibit gene expression in a highly specific and efficient manner. RNAi leads to degradation of target mRNA by introducing into cells a double-stranded RNA, which comprises a sense strand having a sequence homologous to the mRNA of the target gene and an antisense strand having a sequence complementary to the mRNA of the target gene.
For the development of effective therapeutic agents based on siRNA, technical hurdles associated with, for example, stability, cell entry, and silencing efficiency, must be overcome. For example, effective in vivo delivery is challenging because siRNA cannot pass through the cell membrane, due to the negative charge of the phosphate backbone structure. While in the case of in vitro delivery there are many reagents employing cationic lipids and cationic polymers to enhance cell penetration, these reagents are not suitable for use in a therapeutic context.
Pharmaceutical compositions and methods for improving or potentiating siRNA activity, including compositions that enhance siRNA cell entry, are needed to advance this promising technology.
In various aspects and embodiments, the invention provides compounds or agents that potentiate siRNA cellular entry or activity. The present invention further provides methods for identifying such agents. Exemplary agents that act as L-type calcium channel blockers are described herein, and which are demonstrated to potentiate cellular entry of siRNAs.
In one aspect, the disclosure provides pharmaceutical compositions comprising an siRNA, such as a cell-penetrating asymmetric small interfering RNA (cp-asiRNA); and an L-type calcium channel blocker. The L-type calcium channel blocker enhances cellular penetration of the siRNA, leading to more efficient gene silencing. In some embodiments, the L-type calcium channel blocker is dihydropyridine or non-dihydropyridine L-type calcium channel blocker. The pharmaceutical compositions can be formulated for various delivery routes, including topical, pulmonary, and parenteral, for use in methods of treatment.
In other aspects, the invention provides a method of gene silencing in a subject, where the method comprises administering to the subject an effective amount of a small interfering RNA (siRNA); and an L-type calcium channel blocker. The siRNA and the L-type calcium channel blocker can be administered as a single pharmaceutical composition, or in some embodiments the siRNA and the L-type calcium channel blocker are administered as separate pharmaceutical compositions. In some embodiments, the siRNA is an asymmetric siRNA (asiRNA), such as a cell penetrating asiRNA (cp-asiRNA) or a long-antisense asiRNA (lasiRNA). The L-type calcium channel blocker may be a dihydropyridine L-type calcium channel blocker, or a non-dihydropyridine L-type calcium channel blocker. In various embodiments, one or both of the siRNA and L-type calcium channel blocker are formulated for topical, pulmonary, or parenteral delivery.
In another aspect, the disclosure provides a method of screening for a compound to improve the cellular entry or gene silencing activity of an siRNA, such as a cell-penetrating asymmetric small interfering RNA (cp-asiRNA) or a long-antisense asiRNA (lasiRNA). The method may comprise contacting the siRNA with a cell; and contacting a candidate compound with the cell; and detecting or quantifying siRNA in the cell, or in some embodiments, quantifying reduction in expression of a target RNA. By comparing siRNA penetration or activity with a control, compounds or agents that enhance or potentiate siRNA activity or cellular entry can be identified, optionally derivatized, and formulated as pharmaceutical compositions. In some embodiments, compounds can be screened for potentiating activity in high-throughput.
In additional embodiments, the method further comprises selecting a candidate compound that increases cellular penetration or activity of an siRNA (e.g., an asiRNA, e.g. a cp-asiRNA or a lasiRNA). These compounds can be formulated together with the siRNA, or formulated separately, and delivered to patients to potentiate the gene-silencing activity of the siRNA.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as is commonly understood by one of skill in the art to which this invention belongs. All patents and publications referred to herein are incorporated by reference in their entireties.
The present disclosure provides compositions and methods for potentiating gene silencing with siRNAs, as well as methods for preparing such compositions through compound screens. As disclosed herein, L-type calcium channel blockers potentiate the uptake of siRNAs, including cp-asiRNAs or lasiRNAs.
In one aspect, the disclosure provides pharmaceutical compositions comprising an siRNA, such as a cell-penetrating asymmetric small interfering RNA (cp-asiRNA) or a long-antisense asiRNA (lasiRNA); and an L-type calcium channel blocker.
As used herein, the term “RNAi” (RNA interference) refers to a mechanism by which a double-stranded RNA (dsRNA) comprising a first strand having a sequence complementary to the mRNA of a target gene, and a second strand having a sequence complementary to the first strand, is introduced into cells to induce the degradation of the target mRNA. The first strand may be an antisense strand, which refers to a polynucleotide which is substantially, that is about 80%, about 85%, about 90%, about 95%, about 96%, about 97%, about 98%, about 99%, or 100% complementary to a target mRNA. For example, an antisense strand may be complementary, in whole or in part, to a molecule of mRNA (messenger RNA), an RNA sequence that is not mRNA (e.g., microRNA, piwiRNA, tRNA, rRNA and hnRNA) or a sequence of DNA that is either coding or non-coding. The terms “antisense strand” and “guide strand” are used interchangeably herein. In various embodiments, the first strand generally has a length of from about 16 to about 50 nucleotides, such as about 16, 17, 18, 19, 20, 21, 22, 23, 24, and 25 nucleotides. In the first strand, the region complementary to the target nucleic acid may have a length of about 16 to about 31 nucleotides, about 19 to about 25 nucleotides, or about 19 to about 21 nucleotides. In addition, the second strand may be a sense strand, which refers to a polynucleotide that has the same nucleotide sequence, in whole or in part, as the target nucleic acid. The second strand may have a length of about 13 to about 25 nucleotides, about 13 to about 21 nucleotides, or about 16 to about 21 nucleotides. In various embodiments, the second strand may have about 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 or 25 nucleotides.
Asymmetric siRNAs (asiRNAs) are described in US 2012/0238017, which is hereby incorporated by reference in its entirety. In some embodiments, asiRNAs include configurations of “17+2A”, “16+3A” and “15+4A”. A 17+2A siRNA structure refers to a double-stranded siRNA molecule comprising a 19 nucleotide antisense strand and a 17 nucleotide sense strand having a sequence complementary thereto, wherein the 5′ end of the antisense strand is a blunt end and the 3′-end of the antisense strand has a 2 nucleotide overhang. Likewise, the term 16+3A siRNA structure is a double-stranded siRNA molecule comprising a 19 nucleotide antisense strand and a 16 nucleotide sense strand having a sequence complementary thereto, wherein the 5′ end of the antisense strand is a blunt end and the 3′ end of the antisense strand has a 3 nucleotide overhang. A 15+4A siRNA structure is a double-stranded siRNA molecule comprising a 19 nucleotide antisense strand and a 15 nucleotide strand having a sequence complementary thereto, wherein the 5′ end of the antisense strand is a blunt end and the 3′ end of the antisense strand has a 4 nucleotide overhang. asiRNAs provide advantages in gene silencing efficiency, with a reduction in off-target effects by the sense strand.
In some embodiments, one or both ends of the asiRNA comprise an overhang on a 3′ end. In some embodiments, the overhang is a dinucleotide overhang (e.g., dTdT).
In various embodiments, the asiRNA is a cell penetrating asymmetric siRNA or cp-asiRNA. Cp-asiRNAs are described, for example, in U.S. Patent Application Publication No. 2015/0111948, which is hereby incorporated by reference in its entirety. In some embodiments, the cp-asiRNA may internalize and silence a target gene in a cell without any transfection reagents.
In various embodiments, the cp-asiRNA comprises an asiRNA, wherein the phosphate backbone of at least one nucleotide in the nucleic acid molecule is substituted with phosphorothioate or phosphorodithioate, and further comprises a lipophilic compound conjugated thereto to facilitate cellular entry. In various embodiments, the phosphate backbone(s) of nucleotides in a region of the nucleic acid molecule, other than a region complementary to a target nucleic acid, may be substituted with phosphorothioate or phosphorodithioate. In some embodiments, the phosphate backbone of at least one nucleotide in the nucleic acid molecule may be substituted with phosphorothioate. In some embodiments, the lipophilic compound is selected from a lipid, a lipophilic peptide, and a lipophilic protein. The lipid may be at least one selected from cholesterol, tocopherol, and a long-chain fatty acid having 10 or more carbon atoms. In some embodiments, the lipophilic compound is cholesterol, cholestene, cholestane, cholestadiene, bile acid, cholic acid, deoxycholic acid, or dehydrocholic acid. In an embodiment, the lipophilic compound is cholesterol. The lipophilic compound may be conjugated to the end of the first or second strand of the nucleic acid molecule.
In various embodiments, the asiRNA is a long-antisense asiRNA or lasiRNA. LasiRNAs are described, for example, in U.S. Pat. No. 9,637,742, which is hereby incorporated by reference in its entirety.
In various embodiments, the lasiRNA comprises an asiRNA with a first strand of about 21 to about 121 nt length (e.g. about 20 to about 125 nt, or about 20 to about 115 nt, or about 20 to about 105 nt, or about 20 to about 100 nt, or about 20 to about 90 nt, or about 20 to about 80 nt, or about 20 to about 70 nt, or about 20 to about 60 nt, or about 20 to about 50 nt, or about 20 to about 40 nt, or about 20 to about 30 nt, or about 30 to about 125 nt, or about 40 to about 125 nt, or about 50 to about 125 nt, or about 60 to about 125 nt, or about 70 to about 125 nt, or about 80 to about 125 nt, or about 90 to about 125 nt, or about 100 to about 125 nt, or about 24 to about 119 nt length, or about 26-31 nt length, or about 26, or about 27, or about 28, or about 29, or about 30, or about 31 nt length) comprising a region 100% complementary to a target nucleic acid, wherein the region 100% complementary to the target nucleic acid comprises the 19 most 5′ nucleic acids of the first strand; and a second strand of 16 nt length that binds complementarily to the region of the first strand 100% complementary to the target nucleic acid, wherein the second strand binds to the first strand such that the first strand has a double-stranded region to which the second strand binds and a single-stranded region to which the second strand does not bind, and wherein the 5′ end of the first strand and the 3′ end of the second strand form a blunt end.
In various embodiments, the siRNA described herein may be composed of ribonucleotides, deoxyribonucleotides, or both, and may include a variety of modifications providing protection from nucleases or strengthening base pairing interactions. For example, the nucleic acid molecule may comprise one or more nucleotides linked by phorpohorothioate bonds, nucleotides with modifications at the 2′ position, or multicyclic or locked nucleotides.
In some embodiments, the siRNA described herein can employ a variety of oligonucleotide chemistries. Examples of oligonucleotide chemistries include, without limitation, 2′O-Me-modified oligonucleotides, peptide nucleic acid (PNA), locked nucleic acid (LNA), phosphorothioate, and morpholino chemistries, and combinations of any of the foregoing.
In some embodiments, a hydroxyl group at position 2′ of ribose of at least one nucleotide included in the RNAi-inducing double-stranded nucleic acid molecule (e.g., asiRNA or cp-asiRNA) is substituted with at least one selected from a hydrogen atom, a fluorine atom, an —O-alkyl group, an —O-acyl group and an amino group. In some embodiments, the phosphate backbone of at least one nucleotide, included in the nucleic acid molecule, may be substituted with at least one selected from alkylphosphonate form, phosphoroamidate form and boranophosphate form.
In some embodiments, at least one nucleotide included in the nucleic acid molecule may be substituted with at least one selected from LNA (locked nucleic acid), UNA (unlocked nucleic acid), morpholino, and PNA (peptide nucleic acid). In some embodiments, at least one of the nucleotides of the single-stranded region in the first strand may comprise a bulky base analog.
For example, in an embodiment, the nucleic acid molecule comprises one or more multicyclic or locked nucleic acids (LNA). A locked nucleic acid is a modified RNA nucleotide. The ribose moiety of a locked nucleic acid nucleotide is modified with an extra bridge connecting the 2′ oxygen and 4′ carbon. The bridge “locks” the ribose in the 3′-endo (North) conformation, which is often found in the A-form duplexes. Locked nucleic acid nucleotides can be mixed with DNA or RNA residues in the polynucleotide whenever desired and hybridize with DNA or RNA according to Watson-Crick base-pairing rules. The locked ribose conformation enhances base stacking and backbone pre-organization. This significantly increases the hybridization properties (melting temperature) of polynucleotides. Polynucleotides comprising locked nucleic acid nucleotides are nuclease resistant, increasing their chemical stability.
In some embodiments, the nucleic acid molecule may include one or more modified nucleotides. Exemplary modified nucleotides are described in U.S. Pat. No. 8,278,036, which is hereby incorporated by reference in its entirety. For example, the modified nucleotides may be selected from one or more of pseudouridine, N1-methylpseudouridine, 5-methylcytidine, or N6-methyladenosine, 5-hydroxycytidine, 5-hydroxymethylcytidine, 5-carboxycytidine, 5-formylcytidine, 5-hydroxyuridine, 5-hydroxymethyluridine, 5-carboxyuridine, 5-formyluridine, pseudouridine, 2-thiouridine, 4-thiouridine, 5-azauridine, 5-aminouridine, 5-methyluridine, 2-thiopseudouridine, 4-thiopseudouridine, 5-hydroxypseudouridine, 5-methylpseudouridine, 5-aminopseudouridine, pseudoisocytidine, N4-methylcytidine, 2-thiocytidine, 5-azacytidine, 5-aminocytidine, N4-methylpseudoisocytidine, 2-thiopseudoisocytidine, 5-hydroxypseudoisocytidine, 5-aminopseudoisocytidine, 5-methylpseudoisocytidine, 7-deazaadenosine, 6-thioguanosine, 7-deazaguanosine, 8-azaguanosine, 6-thio-7-deazaguanosine, 6-thio-8-azaguanosine, 7-deaza-8-azaguanosine, and 6-thio-7-deaza-8-azaguanosine. In some embodiments, the modified oligonucleotides can be selected and placed such that they do not interfere with base pairing between the two strands of the nucleic acid molecule.
In various embodiments, the present invention provides pharmaceutical compositions comprising an siRNA, such as a cell-penetrating asymmetric small interfering RNA (cp-asiRNA) or a long-antisense asiRNA (lasiRNA); and an L-type calcium channel blocker which can enhance the cellular penetration of the siRNA, leading to more efficient gene silencing.
In various embodiments, the L-type calcium channel blocker is a dihydropyridine L-type calcium channel blocker. Exemplary dihydropyridine L-type calcium channel blocker includes, but is not limited to, amlodipine, aranidipine, azelnidipine, barnidipine, benidipine, cilnidipine, clevidipine, isradipine, efonidipine, felodipine, lacidipine, lercanidipine, manidipine, nicardipine, nifedipine, nilvadipine, nimodipine, nisoldipine, nitrendipine, and pranidipine. In some embodiments, the L-type calcium channel blocker may be selected from:
In other embodiments, the L-type calcium channel blocker is a non-dihydropyridine L-type calcium channel blocker. Exemplary non-dihydropyridine L-type calcium channel blocker includes, but is not limited to, (i) phenylalkylamine and benzothiazepin calcium channel blockers including verapamil, diltiazem, gallopamil, and fendiline; (ii) gabapentinoids including gabapentin and pregabalin; (iii) zixonotide; and (iv) mibefradil, bepridil, flunarizine, and fluspirilene.
In other aspects, the invention provides a method of gene silencing in a subject, where the method comprises administering to the subject an effective amount of a small interfering RNA (siRNA); and an L-type calcium channel blocker. The siRNA and the L-type calcium channel blocker can be administered as a single pharmaceutical composition, or in some embodiments the siRNA and the L-type calcium channel blocker are administered as separate pharmaceutical compositions. In some embodiments, the siRNA is an asymmetric siRNA (asiRNA), such as a cell penetrating asiRNA (cp-asiRNA) or a long-antisense asiRNA (lasiRNA). The L-type calcium channel blocker may be a dihydropyridine L-type calcium channel blocker, or a non-dihydropyridine L-type calcium channel blocker.
Exemplary asiRNA that may be used in connection with the invention are listed in Tables 1-3 below.
In various embodiments, the present invention provides methods for gene silencing of a target gene. In various embodiments, the target gene may be selected from mRNA (messenger RNA), microRNA, piRNA (piwi-interacting RNA), a coding DNA sequence, and a non-coding DNA sequence. In some embodiments, the target gene is mRNA. In additional embodiments, the target gene is mRNA encoding a connective tissue growth factor (CTGF).
In another aspect, the present disclosure further provides methods of screening for a compound to improve, increase or enhance an activity of an siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) comprising contacting the siRNA with a cell; contacting a candidate compound with the cell and/or the siRNA; and detecting the siRNA penetrated into the cell. In some embodiments, the method further comprises comparing an amount of siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) penetrated into the cell with a control to determine whether the penetration of the siRNA into the cell with the candidate compound is increased compared to that without the candidate compound. In some embodiments, the control may be an experimented, known or estimated amount of the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) penetrated without any secondary compound to improve, increase or enhance the cell penetrating activity of the siRNA. The control may be a known value or a predetermined value from a previous experiment. The method may further comprise selecting the candidate compound that increases penetrating of an siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) into the cell as the compound to improve the activity of the siRNA.
In additional embodiments, the method described herein further comprises detecting a gene-silencing activity of an siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) in the cell. The candidate compound that increases the gene-silencing activity of the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) in the cell may be selected as the compound to improve the activity of the siRNA. In further embodiments, the method further comprises comparing a gene-silencing activity of the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) with a control to determine whether the gene-silencing activity of the siRNA with the candidate compound is increased compared to that without the candidate compound. The control may be an experimented, known or estimated gene-silencing activity of the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) without any secondary compound to improve, increase or enhance the gene-silencing activity of the siRNA. The control may be a known value or a predetermined value from a previous experiment.
In various embodiments, the method further comprises preparing a cell culture comprising the cell on a substrate. In some embodiments, the method comprises preparing a cell culture comprising the cell on a plurality of discrete substrates. In some embodiments, the cell on the substrate is contacted with an siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) or a combination of siRNAs. For example, the cell on the substrate may contact a first siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) or a first combination of siRNAs (e.g., asi-RNAs or cp-asiRNAs or lasiRNA) and a second siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) or a second combination of siRNAs (e.g., asi-RNAs or cp-asiRNAs or lasiRNA). In some embodiments, the first siRNA or the first combination of siRNAs contacting the cell on one of the plurality of discrete substrates may be different from the second siRNA or the second combination of siRNAs contacting the cell on another one of the plurality of discrete substrates. A cell on each of the plurality of discrete substrates may contact a different siRNA or combination of siRNAs. In some embodiments, the method comprises comprising contacting a plurality of candidate compounds with the cell on a plurality of substrates, and simultaneously incubating the plurality of candidate compounds with the cell. In additional embodiments, the method comprises simultaneously contacting a plurality of candidate compounds with the cell on a plurality of substrates. In some embodiments, the substrate is a well. In some embodiments, the cell is a human cell.
In some embodiments, the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) is labeled, for example, with a fluorescent dye. The siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) may be detected, for example, by detecting the label. The amount of siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) may be determined, for example, by measuring the amount of the label.
In various embodiments, the present invention provides pharmaceutical compositions which are formulated for various delivery routes, including rectal, buccal, intranasal and transdermal routes, by intra-arterial injection, intravenously, intraperitoneally, parenterally, intramuscularly, subcutaneously, orally, topically, or as an inhalant. In some embodiments, the delivery routes are topical, pulmonary, or parenteral, for use in various methods of treatment as described elsewhere herein. In some embodiments, one or both of the siRNA and L-type calcium channel blocker are formulated for topical, pulmonary, or parenteral delivery. In some embodiments, the pharmaceutical composition is formulated for application to the skin, eyes, lungs, or systemic delivery. In various embodiments, the pharmaceutical compositions are used for in vitro and/or in vivo delivery together with various delivery vehicles, such as, without limitation, liposomes, cationic polymers, antibodies, aptamers or nanoparticles.
In some embodiments, the pharmaceutical compositions described herein may be a pharmaceutical composition for oral administration containing the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) and the L-type calcium channel blocker, and optionally a pharmaceutical excipient suitable for oral administration. In some embodiments, the pharmaceutical composition may exclude a separate delivery vehicle for the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA). In various embodiments, the pharmaceutical composition is formulated in a form suitable for oral administration, i.e. as a solid or a liquid preparation. Suitable solid oral formulations include tablets, capsules, pills, granules, pellets and the like. Suitable liquid oral formulations include solutions, suspensions, dispersions, emulsions, oils and the like.
In some embodiments, the pharmaceutical compositions described herein may be a pharmaceutical composition for injection containing the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) and the L-type calcium channel blocker described herein, and optionally a pharmaceutical excipient suitable for injection. In some embodiments, the pharmaceutical composition may exclude a separate delivery vehicle for the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA).
In some embodiments, the forms in which the pharmaceutical compositions of the present invention may be incorporated for administration by injection include aqueous or oil suspensions, or emulsions, with sesame oil, corn oil, cottonseed oil, or peanut oil, as well as elixirs, mannitol, dextrose, or a sterile aqueous solution, and similar pharmaceutical vehicles. In some embodiments, aqueous solutions in saline are used for injection. Ethanol, glycerol, propylene glycol and liquid polyethylene glycol (and suitable mixtures thereof), cyclodextrin derivatives, and vegetable oils may also be employed. The proper fluidity can be maintained, for example, by the use of a coating, such as lecithin, and in the case of dispersion, by the use of surfactants. The pharmaceutical composition can further comprise various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid and thimerosal.
Sterile injectable solutions are prepared by incorporating an active pharmaceutical ingredient or combination of active pharmaceutical ingredients in the required amounts in the appropriate solvent with various other ingredients as enumerated above, followed by filtered sterilization. Generally, dispersions are prepared by incorporating the various sterilized active ingredients into a sterile vehicle which contains the basic dispersion medium and the required other ingredients from those enumerated above. In the case of sterile powders for the preparation of sterile injectable solutions, certain desirable methods of preparation are vacuum-drying and freeze-drying techniques which yield a powder of the active ingredient plus any additional desired ingredient from a previously sterile-filtered solution thereof.
In some embodiments, the pharmaceutical compositions are administered topically to body surfaces and are thus formulated in a form suitable for topical administration. Suitable topical formulations include, without limitation, gels, ointments, creams, lotions, drops and the like.
In various embodiments, the pharmaceutical composition of the invention further includes a pharmaceutically acceptable carrier or diluent. Pharmaceutically acceptable carriers or diluents are well known to those skilled in the art. The carrier or diluent may be, in various embodiments, a solid carrier or diluent for solid formulations, a liquid carrier or diluent for liquid formulations, or mixtures thereof. In another embodiment, solid carriers/diluents include, but are not limited to, a gum, a starch (e.g. corn starch, pregeletanized starch), a sugar (e.g., lactose, mannitol, sucrose, dextrose), a cellulosic material (e.g. microcrystalline cellulose), an acrylate (e.g. polymethylacrylate), calcium carbonate, magnesium oxide, talc, or mixtures thereof.
In some embodiment, the pharmaceutical compositions are controlled-release compositions, i.e. compositions in which the active agents are released over a period of time after administration. Controlled- or sustained-release compositions include formulation in lipophilic depots (e.g. fatty acids, waxes, oils). In another embodiment, the composition is an immediate-release composition, i.e. a composition in which the active agents are released immediately after administration.
In various embodiments, the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) and the L-type calcium channel blocker described herein are co-administered as a pharmaceutical composition. The terms “co-administration,” “co-administering,” “administered in combination with,” “administering in combination with,” “simultaneous,” and “concurrent,” as used herein, encompass administration of two or more active pharmaceutical ingredients to a human subject so that both active pharmaceutical ingredients and/or their metabolites are present in the human subject at the same time. Co-administration may include simultaneous or time-separated administration in different compositions, administration at different times in separate compositions, or administration in a pharmaceutical composition in which two or more active pharmaceutical ingredients are present. Simultaneous administration in separate compositions and administration in a pharmaceutical composition in which both agents are present is also encompassed in the methods of the invention.
In some embodiments, a pharmaceutical composition or active pharmaceutical ingredient is administered in a single dose. Such administration may be by injection, e.g., intravenous injection, in order to introduce the active pharmaceutical ingredient quickly. However, other routes, including the oral route, may be used as appropriate. A single dose of a pharmaceutical composition may also be used for treatment of an acute condition. In some embodiments, a pharmaceutical composition or active pharmaceutical ingredient is administered in multiple doses. In an embodiment, a pharmaceutical composition is administered in multiple doses. Dosing may be once, twice, three times, four times, five times, six times, or more than six times per day. Dosing may be once a month, once every two weeks, once a week, or once every other day. In other embodiments, a pharmaceutical composition is administered about once per day to about 6 times per day. In some embodiments, a pharmaceutical composition is administered once daily, while in other embodiments, a pharmaceutical composition is administered twice daily, and in other embodiments a pharmaceutical composition is administered three times daily.
In another aspect, the disclosure is related to a method of gene silencing in a subject, comprising administering to the subject an effective amount of the pharmaceutical composition described herein. The methods of gene silencing using siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) are described at least in U.S. Patent Application Publication Nos. 2017/0137828 and 2017/0027837, all of which are incorporated herein by reference in their entirety.
In some embodiments, the present disclosure is related to a method of treating or preventing a connective tissue growth factor (CTGF)-associated disease or disorder in a subject, comprising administering to the subject an effective amount of the pharmaceutical composition comprising an siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) and/or a L-type calcium channel blocker as described herein. In some embodiments, the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) comprises an antisense strand of at least 19 nucleotides in length having a sequence complementarity to CTGF-encoding mRNA and a sense strand of 15 to 17 nucleotides in length having a sequence complementarity to the antisense strand. In various embodiments, the CTGF-associated disease or disorder may be selected from keloid, kidney fibrosis, pachydermatosis, pulmonary fibrosis, hepatic fibrosis, arthritis, hypertension, renal failure, vasculogenesis-related disorder, dermatofibrosis, and cardiovascular system disorder. In some embodiments, the siRNA (e.g., asi-RNA or cp-asiRNA or lasiRNA) may be conjugated to a lipophilic compound and has a pair of nucleic sequences selected from a pair of nucleotide sequences of SEQ ID NOs: 1 and 2, a pair of nucleotide sequences of SEQ ID NOs: 3 and 4, and a pair of nucleotide sequences of SEQ ID NOs: 5 and 6. Additional siRNAs (e.g., asi-RNAs or cp-asiRNAs or lasiRNA) that may be utilized for treating or preventing a CTGF-associated disease or disorder are disclosed in U.S. Patent Publication No. 20150111948, the entire disclosure of which is hereby incorporated by reference.
In some embodiments, the present disclosure is related to a method of treating an ocular condition such as age-related macular degeneration (AMD; e.g., dry or wet AMD) in a subject in need thereof, comprising administering to the subject an effective amount of the pharmaceutical composition described herein, wherein the siRNA (e.g., asi-RNA or cp-asiRNAs or lasiRNA) comprises an antisense strand of at least 19 nucleotides in length having sequence complementarity to a MyD88 mRNA sequence or a TLR3 mRNA sequence and a sense strand of 15 to 17 nucleotides in length having sequence complementarity to the antisense strand, wherein the antisense strand and the sense strand form a complex in which the 5′ end of the antisense strand and the 3′ end of the sense strand form a blunt end. Exemplary siRNAs (e.g., asi-RNAs or cp-asiRNAs or lasiRNA) that may be utilized for treating or preventing AMD are disclosed in U.S. Patent Publication No. 20170137828, the entire disclosure of which is hereby incorporated by reference.
In some embodiments, the present disclosure is related to a method of inhibiting and/or reducing melanin production in a subject, comprising administering to the subject an effective amount of the pharmaceutical composition described herein, wherein the siRNA (e.g., asi-RNA or cp-asiRNA) comprises an antisense strand of at least 19 nucleotides in length having sequence complementarity to a tyrosinase mRNA sequence and a sense strand of 15 to 17 nucleotides in length having sequence complementarity to the antisense strand, wherein the antisense strand and the sense strand form a complex in which the 5′ end of the antisense strand and the 3′ end of the sense strand form a blunt end. Exemplary siRNAs (e.g., asi-RNAs or cp-asiRNAs or lasiRNA) that may be utilized for inhibiting and/or reducing melanin production are disclosed in U.S. Patent Publication No. 20170027837, the entire disclosure of which is hereby incorporated by reference. In some embodiments, the present disclosure further provides methods of treating skin diseases or conditions including, but are not limited to, skin whitening, darkening, or scarring, atopic dermatitis, psoriasis, scleroderma, hair loss, or wrinkled skin.
In various embodiments, the subject being treated by methods of the invention is human.
The embodiments encompassed herein are now described with reference to the following examples. These examples are provided for the purpose of illustration only and the disclosure should in no way be construed as being limited to these examples, but rather should be construed to encompass any and all variations which become evident as a result of the teachings provided herein.
After DMSO toxicity test, 2354 clinical active candidate compounds from a chemical library in Korea Chemical Bank were diluted in distilled water and stored in 96 well sealed plates. Before incubation of cp-asiGFP and chemicals, 4,000 cells of HeLa/GFP stable cell line were seeded in a 96 well plate. After 24 hours of seeding, cp-asiRNA was transfected. After 24 hours of transfection without any transfection reagent, such as lipofectamine or lipofectamine 2000, medium was changed from Opti-MEM (Gibco) to DMEM (Gibco) with 10% FBS (Gibco). Relative fluorescence intensity of cp-asiRNA with chemicals was measured using a multi-well plate reader (
Three hit compounds confirmed by screening were retested to verify the RNA interference effect on cp-asiRNAs. To set the optimal range which is not influenced by cell toxicity, MTT assay was performed. Based on this range (Lethal Dose 50), concentrations of the 3 hit compounds were selected, and the compounds retested within the selected ranges. HeLa/GFP cells were treated with Cp-asiGFPs (100 nM) and the 3 candidate chemicals. After 24 hours of incubation, medium was changed from Opti-MEM (Gibco) to DMEM (Gibco) with 10% FBS (Gibco). After 24 hours of medium change, relative GFP mRNA levels were measured using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) (Panel A in
Cilnidipine, which showed the most gene silencing effect on cp-asiRNAs, was selected. cp-asiRNA targeting GFP (50 nM and 250 nM) and cp-asiRNA targeting Survivin (0.5 μM and 1 μM) with varying concentrations of cilnidipine were added to HeLa/GFP and HeLa cells. After 48 hours, activities of cp-asiRNAs with cilnidipine were analyzed using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The relative GFP and Survivin mRNA levels were measured by dividing the Tubulin levels (internal control) (Panel B in
Amlodipine which was not contained in the chemical library was also tested. This compound has a common structure with DHP and functions as L-type calcium channel blocker. Cp-asiGFP and cp-asisurvivin were treated with Amlodipine into HeLa/GFP and HeLa cells. Their gene silencing effect was measured by qRT-PCR after 48 hours incubation (Panel A in
Another type of calcium channel blocker, like non-DHP L-type calcium channel blocker, is selected for testing. Cp-asiGFP and cp-asisurvivin with Diltiazem and Verapamil were treated into HeLa/GFP and HeLa cells. After 48 hours treated with cp-asiGFP (50 nM, 100 nM) and cp-asisurvivin (0.3 μM, 0.5 μM, 1 μM) with Diltiazem and Verapamil by concentration, reduced GFP and Survivin mRNA levels were observed using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The GFP and Survivin mRNA levels were calculated divided by the Tubulin levels as an internal control. As seen
The effect of another type of calcium channel blocker such as T-type calcium channel blocker was tested. cp-asiGFP (50 nM, 100 nM, 250 nM) and cp-asisurvivin (0.3 μM, 0.5 μM, 1 μM) with Penfluridol and Ethosuximide by concentration were added to HeLa/GFP and HeLa cells. After 48 hours, the relative mRNA levels were analyzed using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). The GFP and Survivin mRNA levels were measured by dividing the Tubulin levels (internal control) (panel A in
To identify the effect of L-type calcium channel blocker on various form of cp-asiRNA, structural change and several chemical modification was applied to cp-asiRNA. The resulting RNA molecule, termed cell penetrating long asymmetric siRNA (cp-lasiRNA) showed efficient target gene silencing. cp-lasiRNA targeting CTGF (cp-lasiCTGF) with Amlodipine and Cilnidipine was treated into HeLa cells. Their gene silencing activity was measured by qRT-PCR (
cp-lasiRNA sequence information (SEQ ID NOs: 145 and 146, “m”: OMe modification, “*”: phosphorothioate (PS) modification, and “chol”: cholesterol modification).
CTGF and GAPDH primer sequence information (SEQ ID NOs: 147-150)
| Number | Date | Country | Kind |
|---|---|---|---|
| 10-2016-0081914 | Jun 2016 | KR | national |
| Filing Document | Filing Date | Country | Kind |
|---|---|---|---|
| PCT/KR2017/006923 | 6/29/2017 | WO | 00 |
| Publishing Document | Publishing Date | Country | Kind |
|---|---|---|---|
| WO2018/004284 | 1/4/2018 | WO | A |
| Number | Name | Date | Kind |
|---|---|---|---|
| 5837258 | Grotendorst | Nov 1998 | A |
| 7078196 | Tuschl et al. | Jul 2006 | B2 |
| 7691997 | Khvorova et al. | Apr 2010 | B2 |
| 7700541 | Tanaka et al. | Apr 2010 | B2 |
| 8410260 | Collin-Djangone et al. | Apr 2013 | B2 |
| 8614309 | Feinstein et al. | Dec 2013 | B2 |
| 8802733 | Ganesan et al. | Aug 2014 | B2 |
| 8822428 | Collin-Djangone et al. | Sep 2014 | B2 |
| 8980273 | Clube | Mar 2015 | B1 |
| 9453226 | Ambati et al. | Sep 2016 | B2 |
| 9637742 | Lee | May 2017 | B2 |
| 9707235 | Ambati | Jul 2017 | B1 |
| 10059949 | Lee et al. | Aug 2018 | B2 |
| 10064801 | Hong et al. | Sep 2018 | B2 |
| 10125362 | Hong | Nov 2018 | B2 |
| 10214744 | Lee | Feb 2019 | B2 |
| 10238749 | Foster | Mar 2019 | B2 |
| 10358648 | Lee et al. | Jul 2019 | B2 |
| 10485885 | Besin | Nov 2019 | B2 |
| 20040138163 | McSwiggen et al. | Jul 2004 | A1 |
| 20040180351 | Giese et al. | Sep 2004 | A1 |
| 20040266707 | Leake et al. | Dec 2004 | A1 |
| 20050119202 | Kreutzer et al. | Jun 2005 | A1 |
| 20050171040 | Polisky | Aug 2005 | A1 |
| 20050282188 | Haeberli et al. | Dec 2005 | A1 |
| 20060069050 | Rana | Mar 2006 | A1 |
| 20060094032 | Fougerolles et al. | May 2006 | A1 |
| 20060105976 | Soutschek et al. | May 2006 | A1 |
| 20060134787 | Zamore et al. | Jun 2006 | A1 |
| 20060142228 | Ford et al. | Jun 2006 | A1 |
| 20060160123 | Quay | Jul 2006 | A1 |
| 20070161596 | McSwiggen | Jul 2007 | A1 |
| 20070218495 | Birmingham et al. | Sep 2007 | A1 |
| 20070275914 | Manoharan et al. | Nov 2007 | A1 |
| 20080125386 | Rana et al. | May 2008 | A1 |
| 20080188430 | Usman et al. | Aug 2008 | A1 |
| 20090004668 | Chen et al. | Jan 2009 | A1 |
| 20090012022 | Milner et al. | Jan 2009 | A1 |
| 20090130751 | Davidson et al. | May 2009 | A1 |
| 20090191625 | Khvorova et al. | Jul 2009 | A1 |
| 20090208564 | Li et al. | Aug 2009 | A1 |
| 20100197023 | Leake et al. | Aug 2010 | A1 |
| 20100254945 | Ge et al. | Oct 2010 | A1 |
| 20100291681 | Khvorova et al. | Nov 2010 | A1 |
| 20110028534 | Shepard et al. | Feb 2011 | A1 |
| 20110054160 | Manoharan | Mar 2011 | A1 |
| 20110237647 | Shirasawa et al. | Sep 2011 | A1 |
| 20110237648 | Khvorova et al. | Sep 2011 | A1 |
| 20110251258 | Samarskv et al. | Oct 2011 | A1 |
| 20110263680 | Khvorova et al. | Oct 2011 | A1 |
| 20110269816 | Kaspar et al. | Nov 2011 | A1 |
| 20120016011 | Pickering et al. | Jan 2012 | A1 |
| 20120238017 | Lee et al. | Sep 2012 | A1 |
| 20130011922 | Quay et al. | Jan 2013 | A1 |
| 20130035368 | Avkin-Nachum et al. | Feb 2013 | A1 |
| 20130115613 | Madiraiu et al. | May 2013 | A1 |
| 20130123342 | Brown | May 2013 | A1 |
| 20130131142 | Libertine et al. | May 2013 | A1 |
| 20130190387 | Feinstein | Jul 2013 | A1 |
| 20130273657 | Lee | Oct 2013 | A1 |
| 20130317080 | Rajeev et al. | Nov 2013 | A1 |
| 20140094501 | Puri et al. | Apr 2014 | A1 |
| 20140227266 | Lee et al. | Aug 2014 | A1 |
| 20140249304 | Lee et al. | Sep 2014 | A1 |
| 20140328903 | Santel et al. | Nov 2014 | A1 |
| 20140350068 | Feinstein et al. | Nov 2014 | A1 |
| 20150111948 | Hong | Apr 2015 | A1 |
| 20150184163 | Wilson et al. | Jul 2015 | A1 |
| 20160017056 | Clube | Jan 2016 | A1 |
| 20160122764 | Chae et al. | May 2016 | A1 |
| 20160235856 | Montefeltro | Aug 2016 | A1 |
| 20160319278 | Khvorova et al. | Nov 2016 | A1 |
| 20160340674 | Jin | Nov 2016 | A1 |
| 20170189541 | Foster | Jul 2017 | A1 |
| 20170298358 | Lee et al. | Oct 2017 | A1 |
| 20170369882 | Khvorova et al. | Dec 2017 | A1 |
| 20190117659 | Friedman | Apr 2019 | A1 |
| 20190185852 | Hussain | Jun 2019 | A1 |
| 20190298842 | Foster | Oct 2019 | A1 |
| Number | Date | Country |
|---|---|---|
| 102719432 | Oct 2012 | CN |
| 2631291 | Aug 2013 | EP |
| 2012502991 | Feb 2012 | JP |
| 101207561 | Dec 2012 | KR |
| 10-1595152 | Feb 2016 | KR |
| WO0244321 | Jun 2002 | WO |
| WO02055693 | Jul 2002 | WO |
| WO2005062937 | Jul 2005 | WO |
| WO 2005063213 | Jul 2005 | WO |
| WO2005079533 | Sep 2005 | WO |
| WO 2006084005 | Aug 2006 | WO |
| WO2007002470 | Feb 2007 | WO |
| WO2007128477 | Nov 2007 | WO |
| WO2008109377 | Sep 2008 | WO |
| WO2009020344 | Feb 2009 | WO |
| WO2009029688 | Mar 2009 | WO |
| WO2009029690 | Mar 2009 | WO |
| WO2009078685 | Jun 2009 | WO |
| WO2009105260 | Aug 2009 | WO |
| WO2010033247 | Mar 2010 | WO |
| WO2010090762 | Aug 2010 | WO |
| WO201119887 | Sep 2011 | WO |
| WO2012078536 | Jun 2012 | WO |
| WO2012118911 | Sep 2012 | WO |
| WO2014043291 | Mar 2014 | WO |
| WO2015002513 | Jan 2015 | WO |
| WO2015015498 | Feb 2015 | WO |
| WO 2015171641 | Nov 2015 | WO |
| WO2017017523 | Feb 2017 | WO |
| WO2017085550 | May 2017 | WO |
| WO2017134525 | Aug 2017 | WO |
| WO2017134526 | Aug 2017 | WO |
| WO2017178883 | Aug 2017 | WO |
| WO2018004284 | Jan 2018 | WO |
| WO2018146557 | Aug 2018 | WO |
| Entry |
|---|
| Ambati et al., “Mechanisms of Age-Related Macular Degeneration,” Neuron, 75: 26-39 (2012). |
| Bolcato-Bellemin et al., “Sticky overhangs enhance siRNA-mediated gene silencing,” PNAS, vol. 104, No. 41, pp. 16050-16055 (2007). |
| Bramsen et al., “Improved silencing properties using small internally segmented interfering RNAs,” Nucleic Acids Research, (2007), pp. 5886-5897, vol. 35. |
| Bushati et al., “MicroRNAs in Neurodegeneration,” Current Opin Neurobiol, 18: 292-296 (2008). |
| Caplen et al., “Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems,” Proc. Natl. Acad. Sci. USA, (2001), pp. 9742-9747, vol. 98, No. 17. |
| Chang et al., “Structural diversity repertoire of gene silencing small interfering RNAs,” Nucleic acid therapeutics, (2011), 21(3), 125-131. |
| Chang et al., “The design, preparation, and evaluation of asymmetric small interfering RNA for specific gene silencing in mammalian cells,” Methods Mol Biol. 2013; 942:135-52. |
| Chang et al., “Asymmetric shorter-duplex siRNA structures trigger efficient gene silencing with reduced nonsoecific effects,” Mol Ther, (2009), 17(4):725-732. |
| Chiu et al., “siRNA Function in RNAi: A Chemical Modification Analysis,” RNA, 9: 1034-1048 (2003). |
| Doench et al., “siRNAs Can Function as miRNAs,” Gene Dev, 17(4): 438-442 (2003). |
| Doench et al., “Specificity of MicroRNA Target Selection in Translation Repression,” Gene Dev, 18: 504-511 (2004). |
| Elbashir et al., “Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells,” Nature, (2001), 411:494-498. |
| Elbashir et al., “Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate,” The EMBO Journal, (2001), pp. 6877-6888, vol. 20, No. 23. |
| Fire, “RNA-triggered gene silencing,” Trends in Genetics, (1999), vol. 15, No. 9, pp. 358-363. |
| Fire et al., “Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans,” Nature, (1998), pp. 806-811, vol. 391. |
| Grimm, “Small silencing RNAs: State of the art,” Advanced Drug Delivery Reviews, 61: 672-703 (2009). |
| Hammond et al., “Post-Transcriptional Gene Silencing by Double-Stranded RNA,” Nature Reviews Genetics, (2001), vol. 2: 110-119. |
| Hong et al., “Effect of the guide strand 3′-end structure on the gene-silencing potency of asymmetric siRNA,” Biochemical Journal, (2014), 461(3): 427-434. |
| Huang et al., “Targeting the ANGPT-TIE2 Pathway in Malignancy,” Nat Rev Cancer, 10: 575-585 (2010). |
| Hwang, “Development of Cell-Penetrating Asymmetric Interfering RNA Targeting Connective Tissue Growth Factor,” Journal of Investigative Dermatology, (2016), 136(11), 2305-2313. |
| Jackson et al., “Expression Profiling Reveals Off-Target Gene Regulation by RNAi,” Nat Biotechnol, 21: 635-637 (2003). |
| Jang et al., “Gene Delivery From Polymer Scaffolds for Tissue Engineering,” Expert Rev Med Devic, 1(1): 127-138 (2004). |
| Jeong et al., “siRNA conjugate delivery systems,” Bioconjugate Chem, 20:5-14 (2009). |
| Jo et al., “Selection and optimization of asymmetric siRNA targeting the human c-MET gene,” Mol Cell, 32:(6) 543-548 (2011). |
| Joshi, et al., “siRNA: novel therapeutics from functional genomics,” Biotechnology and Genetic Enginnering Reviews (2014) vol. 30, No. 1, pp. 1-30. |
| Kelly et al., “Cell Type-Specific Regulation of Angiogenic Growth Factor Gene Expression and Induction of Angiogenesis in Nonischemic Tissue by a Constitutively Active Form of Hypoxia-inducible Factor 1,” Circ Res, 93: 1074-1081 (2003). |
| Kim et al., “Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy,” Nature Biotechnology, 23: 222-226 (2005). |
| Kore et al., “Chemical modification of synthetic RNAi agents and in vivo delivery techniques,” Curr Bioactive Compounds, 4:6-14 (2008). |
| Kubo et al., “Modified 27nt dsRNAs with Dramatically Enhanced Stability in Serum and Long-Term RNAi Activity,” Oligonucleotides, 17:445-464 (2007). |
| Kulkarni et al., “Evidence of Off-Target Effects Associated with Long dsRNAs in Drosophila melanogaster Cell-Based Assays,” Nat Methods, 3: 833-838 (2006). |
| Lee et al., “Asymmetric RNA Duplexes as Next Generation RNAi Inducers,” Gene Silencing: Theory, Techniques and Applications, (2010), pp. 343-348. |
| Li et al., “Inhibition of connective tissue growth factor by siRNA prevents liver fibrosis in rats,” The Journal of Gene Medicine, (2006), 8:889-900. |
| Lima et al., “Human Dicer Binds Short Single-strand and Double-strand RNA with High Affinity and Interacts with Different Regions of the Nucleic Acids,” The Journal of Biological Chemistry (2009), 284:2535-2548. |
| Luo et al., “Inhibition of Connective Tissue Growth Factor by Small Interfering RNA Prevents Renal Fibrosis in Rats Undergoing Chronic Allograft Nephropathy,” Transplantation Proceedings, (2008), 40:2365-2369. |
| Marques et al., “A structural basis for discriminating between self and nonself double-stranded RNAs in mammalian cells,” Nature Biotechnology, 24: 559-565 (2006). |
| Martinez et al., “Singe-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi,” Cell, 110: 563-574 (2002). |
| Opalinska et al., “Nucleic-acid Therapeutics: Basic Principles and Recent Applications,” Nature Rev, 1 (7): 503-514 (2002). |
| Paroo et al., “Challenges for RNAi in vivo,” Trends in Biotech, 22(8): 390-394 (2004). |
| Patel et al., “A Novel Protective Role for the Innate Immunity Toll-Like Receptor 3 (TLR3) in the Retina via Stat3,” Mol Cell Neurosci, 63: 38-48 (2014). |
| Raouane et al., “Lipid conjugated oligonucleotides: a useful strategy for delivery,” Bioconjugate Chem, 23:1091-104 (2012). |
| Rose et al., “Functional Polarity is Introduced by Dicer Processing of Short Substrate RNAs,” Nucleic Acids Res, 33: 4140-4156 (2005). |
| Sano et al., “Effect of asymmetric terminal structures of short RNA duplexes on the RNA interference activity and strand selection,” Nucleic Acids Research (2008), 36: 5812-5821. |
| Sharp et al., “RNA-interference-2001,” Genes & Development, (2001), 15:485-490. |
| Sioud et al., “Cationic liposome-mediated delivery of siRNAs in adult mice,” Biochem Biophys Res Commun. Dec. 26, 2003; 312(4):1220-5. |
| Sisco et al., “Antisense Inhibition of Connective Tissue Growth Factor (CTGF/CCN2) mRNA Limits Hypertrophic Scarring Without Affecting Wound Healing in Vivo,” Wound Repair Regen, 16: 661-673 (2008). |
| Song et al., “The Crystal Structure of the Argonaute2 PAZ Domain Reveals an RNA Binding Motif in RNAi Effector Complexes,” Nat Struct Biol, 10(12): 1026-1032 (2003). |
| Soutschek et al., “Therapeutic Silencing of an Endogenous Gene by Systemic Adminstration of Modified siRNAs,” Nature, 432: 173-178 (2004). |
| Sun et al., “Asymmetic RNA duplexes mediate RNA interference in mammalian cells,” Nature Biotechnology, 26: 1379-1382 (2008). |
| Ui-Tei et al., “Essential Notes Regarding the Design of Functional siRNAs for Efficient Mammalian RNAi,” J Biomed Biotechnol, 2006; 2006(4):65052. doi: 10.1155/JBB/2006/65052. |
| Ui-Tei et al., “Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference,” (2004), Nucleic Acids Research, vol. 32, pp. 936-948. |
| Vasdudevan et al., “Switching from Repression to Activation: MicroRNAs Can Up-Regulate Translation,” Science, 318: 1931-1934 (2007). |
| Wang et al., “Nucleation, Propagation and Cleavage of Target RNAs in Ago Silencing Complexes,” Nature, 461: 754-762 (2009). |
| Yang et al., “HENI recognizes 21-24 nt small RNA duplexes and deposits a methyl group onto the 2′ OH of the 3′ terminal nucleotide,” Nucleic Acids Research, 34: 667-675 (2006). |
| Yuan et al., “Asymmetric siRNA: New Strategy to Improve Specificity and Reduce Off-Target Gene Expression”, Human Gene Therapy 23:521-532 (2013). |
| Zamore, “RNA interference: listening to the sound of silence,” Nature Structural Biology, (2001), 8(9):746-750. |
| Juliano, “The delivery of therapeutic oligonucleotides,” Nucleic Acids Research, vol. 14, No. 14, pp. 6518-6548, Apr. 15, 2016. |
| International Search Report & Written Opinion, PCT Appl. No. PCT/KR2017/006923, dated Oct. 11, 2017, 12 pages. |
| Number | Date | Country | |
|---|---|---|---|
| 20190321388 A1 | Oct 2019 | US |
