Claims
- 1. A short interfering RNA (siRNA) molecule that down-regulates expression of hepatitis B virus (HBV) RNA, wherein said siRNA comprises nucleotide sequence complementary to said HBV RNA or a portion thereof.
- 2. The siNA molecule of claim 1, wherein said siRNA molecule is double stranded.
- 3. The siRNA molecule of claim 2, wherein each strand of said siRNA comprises about 21 nucleotides.
- 4. The siRNA molecule of claim 2, wherein each strand of said siRNA comprises a 3′-nucleotide overhang.
- 5. The siRNA molecule of claim 4, wherein said 3′-nucleotide overhang comprises two nucleotides.
- 6. The siRNA molecule of claim 1, wherein said siRNA molecule is single stranded.
- 7. The siRNA molecule of claim 1, wherein said siRNA molecule comprises one or more chemically modified nucleotides.
- 8. The siRNA molecule of claim 7, wherein said chemically modified nucleotide is a 2′-O-alkyl nucleotide.
- 9. The siRNA molecule of claim 8, wherein said 2′-O-alkyl nucleotides is a 2′-O-methyl nucleotide.
- 10. The siRNA molecule of claim 8, wherein said 2′-O-alkyl nucleotides is a 2′-O-allyl nucleotide.
- 11. The siRNA molecule of claim 7, wherein said chemically modified nucleotide is a 2′-deoxy-2′-fluoro nucleotide.
- 12. The siRNA molecule of claim 7, wherein said chemically modified nucleotide is a 2′-deoxy nucleotide.
- 13. The siRNA molecule of claim 7, wherein said chemically modified nucleotide comprises one or more phosphorothioate internucleotide linkages.
- 14. The siRNA molecule of claim 7, wherein said chemically modified nucleotide is a 2′-O-alkyl nucleotide, 2′-deoxy-2′-fluoro nucleotide, 2′-deoxy nucleotide, phosphorothioate containing nucleotide, or any combination thereof.
- 15. The siRNA molecule of claim 7, wherein said siRNA comprises one or more 2′-O-alkyl and one or more 2′-deoxy-2′-fluoro nucleotides.
- 16. The siRNA molecule of claim 7, wherein said siRNA comprises one or more 2′-deoxy and one or more 2′-deoxy-2′-fluoro nucleotides.
- 17. The siRNA molecule of claim 1, wherein said siRNA comprises a terminal cap modification.
- 18. The siRNA molecule of claim 17, wherein said terminal cap modification comprises an inverted abasic moiety.
- 19. The siRNA molecule of claim 17, wherein said terminal cap modification is at the 3′-end of said siRNA.
- 20. The siRNA molecule of claim 1, wherein said siRNA is conjugated to a biologically active molecule.
- 21. The siRNA molecule of claim 20, wherein said siRNA molecule is conjugated to said biologically active molecule via a biodegradable linker.
- 22. The siRNA molecule of claim 20, wherein said biologically active molecule comprises a vitamin.
- 23. The siRNA molecule of claim 20, wherein said biologically active molecule comprises an antibody.
- 24. The siRNA molecule of claim 20, wherein said biologically active molecule comprises a hormone.
Parent Case Info
[0001] This patent application is a continuation of International Application No. PCT/US02/09187, with international filing date of Mar. 26, 2002, published in English under PCT Article 21(2), which claims the benefit of Macejak et al., USSN (60/296,876), filed Jun. 8, 2001, Macejak et al., USSN (60/335,059), filed Oct. 24, 2001, Morrissey et al., USSN (60/337,055), filed Dec. 5, 2001, Beigelman et al., USSN (60/358,580), filed Feb. 20, 2002, Beigelman et al., USSN (60/363,124), filed Mar. 11, 2002, and which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/817,879), filed Mar. 26, 2001, which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/740,332), filed Dec. 18, 2000, which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/611,931), filed Jul. 7, 2000, which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/504,321), filed Feb. 15, 2000, which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/274,553), filed Mar. 23, 1999, which is a continuation-in-part of Blatt et al., USSN (Ser. No. 09/257,608), filed Feb. 24, 1999 (abandoned), which claims the benefit of Blatt et al., USSN (60/100,842), filed Sep. 18, 1998, and McSwiggen et al., USSN (60/083,217) filed Apr. 27, 1998. This patent application is also a continuation-in-part of Draper et al., USSN (Ser. No. 09/877,478) filed Jun. 8, 2001, which is a continuation-in-part of Draper et al., USSN (Ser. No. 09/696,347), filed Oct. 24, 2000, which is a continuation-in-part of Draper et al., USSN (Ser. No. 09/636,385), filed Aug. 9, 2000, which is a continuation-in-part of Draper et al., USSN (Ser. No. 09/531,025), filed Mar. 20, 2000, which is a continuation-in-part of Draper et al., USSN (Ser. No. 09/436,430), filed Nov. 8, 1999, which is a continuation of Draper et al., USSN (Ser. No. 08/193,627), filed Feb. 7, 1994, now U.S. Pat. No. 6,017,756, which is a continuation of Draper et al., USSN (Ser. No. 07/882,712), filed May 14, 1992, now abandoned. All of these listed applications are hereby incorporated by reference herein in their entireties, including the drawings.
Provisional Applications (7)
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60296876 |
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60363124 |
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60100842 |
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60083217 |
Apr 1998 |
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PCT/US02/09187 |
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Continuation in Parts (11)
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